This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGTGGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 90.44 s (29 us/read; 2.05 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 25,435 (0.8%) Reads that were too short: 16,603 (0.5%) Reads written (passing filters): 8,832 (0.3%) Total basepairs processed: 931,490,553 bp Total written (filtered): 2,296,963 bp (0.2%) === Adapter 1 === Sequence: TGTGGC; Type: regular 5'; Length: 6; Trimmed: 25435 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 78 755.5 0 78 7 482 755.5 0 482 8 136 755.5 0 136 9 5144 755.5 0 5144 10 20 755.5 0 20 11 6 755.5 0 6 12 21 755.5 0 21 13 8 755.5 0 8 14 13 755.5 0 13 15 6 755.5 0 6 16 13 755.5 0 13 17 9 755.5 0 9 18 9 755.5 0 9 19 21 755.5 0 21 20 6 755.5 0 6 21 13 755.5 0 13 22 7 755.5 0 7 23 11 755.5 0 11 24 8 755.5 0 8 25 14 755.5 0 14 26 8 755.5 0 8 27 9 755.5 0 9 28 13 755.5 0 13 29 8 755.5 0 8 30 22 755.5 0 22 31 8 755.5 0 8 32 20 755.5 0 20 33 13 755.5 0 13 34 10 755.5 0 10 35 10 755.5 0 10 36 12 755.5 0 12 37 8 755.5 0 8 38 10 755.5 0 10 39 7 755.5 0 7 40 8 755.5 0 8 41 12 755.5 0 12 42 9 755.5 0 9 43 6 755.5 0 6 44 8 755.5 0 8 45 17 755.5 0 17 46 4 755.5 0 4 47 5 755.5 0 5 48 11 755.5 0 11 49 18 755.5 0 18 50 26 755.5 0 26 51 10 755.5 0 10 52 179 755.5 0 179 53 11 755.5 0 11 54 15 755.5 0 15 55 19 755.5 0 19 56 11 755.5 0 11 57 76 755.5 0 76 58 6 755.5 0 6 59 10 755.5 0 10 60 14 755.5 0 14 61 8 755.5 0 8 62 23 755.5 0 23 63 7 755.5 0 7 64 13 755.5 0 13 65 5 755.5 0 5 66 14 755.5 0 14 67 6 755.5 0 6 68 9 755.5 0 9 69 10 755.5 0 10 70 6 755.5 0 6 71 50 755.5 0 50 72 42 755.5 0 42 73 34 755.5 0 34 74 14 755.5 0 14 75 16 755.5 0 16 76 8 755.5 0 8 77 8 755.5 0 8 78 8 755.5 0 8 79 5 755.5 0 5 80 8 755.5 0 8 81 5 755.5 0 5 82 4 755.5 0 4 83 7 755.5 0 7 84 6 755.5 0 6 85 4 755.5 0 4 86 2 755.5 0 2 87 6 755.5 0 6 88 2 755.5 0 2 89 3 755.5 0 3 90 3 755.5 0 3 91 4 755.5 0 4 92 13 755.5 0 13 93 14 755.5 0 14 94 5 755.5 0 5 95 3 755.5 0 3 96 10 755.5 0 10 97 6 755.5 0 6 98 2 755.5 0 2 99 4 755.5 0 4 100 8 755.5 0 8 101 6 755.5 0 6 102 5 755.5 0 5 103 8 755.5 0 8 104 5 755.5 0 5 105 16 755.5 0 16 106 8 755.5 0 8 107 4 755.5 0 4 108 5 755.5 0 5 109 5 755.5 0 5 110 5 755.5 0 5 111 8 755.5 0 8 112 8 755.5 0 8 113 7 755.5 0 7 114 4 755.5 0 4 115 7 755.5 0 7 116 4 755.5 0 4 117 18 755.5 0 18 118 13 755.5 0 13 119 8 755.5 0 8 120 11 755.5 0 11 121 10 755.5 0 10 122 9 755.5 0 9 123 1 755.5 0 1 124 13 755.5 0 13 125 20 755.5 0 20 126 7 755.5 0 7 127 12 755.5 0 12 128 22 755.5 0 22 129 9 755.5 0 9 130 33 755.5 0 33 131 328 755.5 0 328 132 186 755.5 0 186 133 123 755.5 0 123 134 24 755.5 0 24 135 6 755.5 0 6 136 4 755.5 0 4 137 12 755.5 0 12 138 204 755.5 0 204 139 244 755.5 0 244 140 5 755.5 0 5 141 4 755.5 0 4 142 11 755.5 0 11 143 4 755.5 0 4 144 3 755.5 0 3 145 4 755.5 0 4 146 7 755.5 0 7 147 3 755.5 0 3 148 6 755.5 0 6 149 5 755.5 0 5 150 2 755.5 0 2 151 10 755.5 0 10 152 4 755.5 0 4 153 11 755.5 0 11 154 7 755.5 0 7 155 2 755.5 0 2 156 4 755.5 0 4 157 1 755.5 0 1 159 9 755.5 0 9 160 1 755.5 0 1 161 3 755.5 0 3 162 7 755.5 0 7 163 6 755.5 0 6 164 5 755.5 0 5 165 4 755.5 0 4 166 2 755.5 0 2 167 4 755.5 0 4 168 6 755.5 0 6 169 3 755.5 0 3 170 3 755.5 0 3 171 5 755.5 0 5 172 4 755.5 0 4 173 4 755.5 0 4 174 8 755.5 0 8 175 4 755.5 0 4 176 6 755.5 0 6 177 5 755.5 0 5 178 17 755.5 0 17 179 17 755.5 0 17 180 5 755.5 0 5 181 5 755.5 0 5 182 1 755.5 0 1 183 7 755.5 0 7 184 1 755.5 0 1 185 6 755.5 0 6 186 5 755.5 0 5 187 6 755.5 0 6 188 13 755.5 0 13 189 15 755.5 0 15 190 10 755.5 0 10 191 2 755.5 0 2 192 6 755.5 0 6 193 4 755.5 0 4 194 2 755.5 0 2 195 5 755.5 0 5 196 3 755.5 0 3 197 5 755.5 0 5 198 8 755.5 0 8 199 6 755.5 0 6 200 10 755.5 0 10 201 6 755.5 0 6 202 10 755.5 0 10 203 25 755.5 0 25 204 26 755.5 0 26 205 106 755.5 0 106 206 10 755.5 0 10 207 5 755.5 0 5 208 7 755.5 0 7 209 7 755.5 0 7 210 10 755.5 0 10 211 5 755.5 0 5 212 40 755.5 0 40 213 90 755.5 0 90 214 261 755.5 0 261 215 12 755.5 0 12 216 3 755.5 0 3 217 5 755.5 0 5 218 12 755.5 0 12 219 5 755.5 0 5 220 5 755.5 0 5 221 1 755.5 0 1 222 6 755.5 0 6 223 6 755.5 0 6 224 1 755.5 0 1 225 11 755.5 0 11 226 5 755.5 0 5 227 8 755.5 0 8 228 5 755.5 0 5 229 3 755.5 0 3 230 6 755.5 0 6 231 9 755.5 0 9 232 3 755.5 0 3 233 10 755.5 0 10 234 6 755.5 0 6 235 17 755.5 0 17 236 23 755.5 0 23 237 97 755.5 0 97 238 75 755.5 0 75 239 7 755.5 0 7 240 2 755.5 0 2 241 3 755.5 0 3 242 7 755.5 0 7 243 10 755.5 0 10 244 20 755.5 0 20 245 35 755.5 0 35 246 44 755.5 0 44 247 20 755.5 0 20 248 5 755.5 0 5 249 8 755.5 0 8 250 31 755.5 0 31 251 9 755.5 0 9 252 11 755.5 0 11 253 31 755.5 0 31 254 28 755.5 0 28 255 22 755.5 0 22 256 48 755.5 0 48 257 32 755.5 0 32 258 31 755.5 0 31 259 69 755.5 0 69 260 414 755.5 0 414 261 1141 755.5 0 1141 262 2401 755.5 0 2401 263 153 755.5 0 153 264 7 755.5 0 7 265 59 755.5 0 59 266 10 755.5 0 10 267 12 755.5 0 12 268 18 755.5 0 18 269 26 755.5 0 26 270 22 755.5 0 22 271 23 755.5 0 23 272 49 755.5 0 49 273 100 755.5 0 100 274 22 755.5 0 22 275 28 755.5 0 28 276 75 755.5 0 75 277 76 755.5 0 76 278 49 755.5 0 49 279 62 755.5 0 62 280 145 755.5 0 145 281 68 755.5 0 68 282 80 755.5 0 80 283 70 755.5 0 70 284 88 755.5 0 88 285 172 755.5 0 172 286 2298 755.5 0 2298 287 3617 755.5 0 3617 288 3644 755.5 0 3644 289 18 755.5 0 18 290 3 755.5 0 3 291 4 755.5 0 4 292 4 755.5 0 4 293 6 755.5 0 6 294 7 755.5 0 7 295 5 755.5 0 5 296 36 755.5 0 36 297 8 755.5 0 8 298 17 755.5 0 17 299 38 755.5 0 38 300 116 755.5 0 116 301 3 755.5 0 3 Finished in 0.42 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 8,832 Reads with adapters: 5,344 (60.5%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 5,341 (60.5%) Total basepairs processed: 2,296,963 bp Total written (filtered): 1,468,496 bp (63.9%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 5344 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 1 0.0 3 0 0 1 16 36 0.0 3 0 36 17 5274 0.0 3 5144 126 4 18 13 0.0 3 1 10 2 19 2 0.0 3 1 0 0 1 20 10 0.0 3 0 0 0 10 41 1 0.0 3 0 0 0 1 61 1 0.0 3 0 0 0 1 64 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.23 s (43 us/read; 1.39 M reads/minute). === Summary === Total reads processed: 5,341 Reads with adapters: 440 (8.2%) Reads written (passing filters): 5,341 (100.0%) Total basepairs processed: 1,468,496 bp Total written (filtered): 1,467,175 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 440 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 75.9% C: 3.2% G: 20.7% T: 0.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 439 83.5 0 439 4 1 20.9 0 1