This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGTTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 89.91 s (29 us/read; 2.07 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 29,929 (1.0%) Reads that were too short: 5,718 (0.2%) Reads written (passing filters): 24,211 (0.8%) Total basepairs processed: 931,490,553 bp Total written (filtered): 6,396,224 bp (0.7%) === Adapter 1 === Sequence: TCGTTA; Type: regular 5'; Length: 6; Trimmed: 29929 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 155 755.5 0 155 7 275 755.5 0 275 8 18054 755.5 0 18054 9 209 755.5 0 209 10 5 755.5 0 5 11 3 755.5 0 3 12 3 755.5 0 3 13 3 755.5 0 3 14 4 755.5 0 4 15 4 755.5 0 4 16 5 755.5 0 5 17 2 755.5 0 2 18 1 755.5 0 1 19 6 755.5 0 6 20 9 755.5 0 9 21 6 755.5 0 6 22 5 755.5 0 5 23 7 755.5 0 7 24 5 755.5 0 5 25 4 755.5 0 4 26 10 755.5 0 10 27 3 755.5 0 3 28 5 755.5 0 5 29 9 755.5 0 9 30 8 755.5 0 8 31 4 755.5 0 4 32 5 755.5 0 5 33 7 755.5 0 7 34 5 755.5 0 5 35 2 755.5 0 2 36 6 755.5 0 6 37 8 755.5 0 8 38 49 755.5 0 49 39 9 755.5 0 9 40 12 755.5 0 12 41 4 755.5 0 4 42 6 755.5 0 6 43 10 755.5 0 10 44 10 755.5 0 10 45 5 755.5 0 5 46 17 755.5 0 17 47 10 755.5 0 10 48 5 755.5 0 5 49 5 755.5 0 5 50 9 755.5 0 9 51 1 755.5 0 1 52 10 755.5 0 10 53 14 755.5 0 14 54 70 755.5 0 70 55 7 755.5 0 7 56 10 755.5 0 10 57 3 755.5 0 3 58 7 755.5 0 7 59 10 755.5 0 10 60 14 755.5 0 14 61 8 755.5 0 8 62 16 755.5 0 16 63 16 755.5 0 16 64 6 755.5 0 6 65 16 755.5 0 16 66 16 755.5 0 16 67 25 755.5 0 25 68 14 755.5 0 14 69 5 755.5 0 5 70 8 755.5 0 8 71 9 755.5 0 9 72 10 755.5 0 10 73 5 755.5 0 5 74 14 755.5 0 14 75 12 755.5 0 12 76 10 755.5 0 10 77 8 755.5 0 8 78 9 755.5 0 9 79 7 755.5 0 7 80 21 755.5 0 21 81 8 755.5 0 8 82 5 755.5 0 5 83 13 755.5 0 13 84 12 755.5 0 12 85 9 755.5 0 9 86 4 755.5 0 4 87 9 755.5 0 9 88 11 755.5 0 11 89 10 755.5 0 10 90 9 755.5 0 9 91 31 755.5 0 31 92 11 755.5 0 11 93 13 755.5 0 13 94 22 755.5 0 22 95 185 755.5 0 185 96 41 755.5 0 41 97 107 755.5 0 107 98 97 755.5 0 97 99 133 755.5 0 133 100 117 755.5 0 117 101 19 755.5 0 19 102 31 755.5 0 31 103 10 755.5 0 10 104 10 755.5 0 10 105 9 755.5 0 9 106 14 755.5 0 14 107 12 755.5 0 12 108 28 755.5 0 28 109 22 755.5 0 22 110 11 755.5 0 11 111 55 755.5 0 55 112 64 755.5 0 64 113 373 755.5 0 373 114 6 755.5 0 6 115 10 755.5 0 10 116 18 755.5 0 18 117 18 755.5 0 18 118 12 755.5 0 12 119 8 755.5 0 8 120 27 755.5 0 27 121 24 755.5 0 24 122 17 755.5 0 17 123 13 755.5 0 13 124 12 755.5 0 12 125 14 755.5 0 14 126 20 755.5 0 20 127 22 755.5 0 22 128 13 755.5 0 13 129 59 755.5 0 59 130 380 755.5 0 380 131 23 755.5 0 23 132 43 755.5 0 43 133 38 755.5 0 38 134 83 755.5 0 83 135 33 755.5 0 33 136 46 755.5 0 46 137 21 755.5 0 21 138 25 755.5 0 25 139 11 755.5 0 11 140 46 755.5 0 46 141 9 755.5 0 9 142 11 755.5 0 11 143 7 755.5 0 7 144 9 755.5 0 9 145 15 755.5 0 15 146 27 755.5 0 27 147 25 755.5 0 25 148 17 755.5 0 17 149 7 755.5 0 7 150 10 755.5 0 10 151 9 755.5 0 9 152 7 755.5 0 7 153 12 755.5 0 12 154 5 755.5 0 5 155 13 755.5 0 13 156 55 755.5 0 55 157 25 755.5 0 25 158 18 755.5 0 18 159 21 755.5 0 21 160 15 755.5 0 15 161 19 755.5 0 19 162 13 755.5 0 13 163 4 755.5 0 4 164 24 755.5 0 24 165 16 755.5 0 16 166 56 755.5 0 56 167 36 755.5 0 36 168 34 755.5 0 34 169 31 755.5 0 31 170 6 755.5 0 6 171 21 755.5 0 21 172 22 755.5 0 22 173 40 755.5 0 40 174 27 755.5 0 27 175 38 755.5 0 38 176 41 755.5 0 41 177 7 755.5 0 7 178 11 755.5 0 11 179 25 755.5 0 25 180 40 755.5 0 40 181 102 755.5 0 102 182 47 755.5 0 47 183 24 755.5 0 24 184 28 755.5 0 28 185 47 755.5 0 47 186 61 755.5 0 61 187 27 755.5 0 27 188 9 755.5 0 9 189 13 755.5 0 13 190 11 755.5 0 11 191 16 755.5 0 16 192 46 755.5 0 46 193 293 755.5 0 293 194 162 755.5 0 162 195 78 755.5 0 78 196 137 755.5 0 137 197 55 755.5 0 55 198 24 755.5 0 24 199 92 755.5 0 92 200 82 755.5 0 82 201 113 755.5 0 113 202 85 755.5 0 85 203 17 755.5 0 17 204 29 755.5 0 29 205 34 755.5 0 34 206 36 755.5 0 36 207 32 755.5 0 32 208 35 755.5 0 35 209 20 755.5 0 20 210 21 755.5 0 21 211 27 755.5 0 27 212 42 755.5 0 42 213 37 755.5 0 37 214 28 755.5 0 28 215 64 755.5 0 64 216 71 755.5 0 71 217 100 755.5 0 100 218 35 755.5 0 35 219 45 755.5 0 45 220 104 755.5 0 104 221 166 755.5 0 166 222 358 755.5 0 358 223 86 755.5 0 86 224 22 755.5 0 22 225 27 755.5 0 27 226 34 755.5 0 34 227 42 755.5 0 42 228 53 755.5 0 53 229 28 755.5 0 28 230 28 755.5 0 28 231 17 755.5 0 17 232 19 755.5 0 19 233 13 755.5 0 13 234 20 755.5 0 20 235 23 755.5 0 23 236 35 755.5 0 35 237 63 755.5 0 63 238 132 755.5 0 132 239 73 755.5 0 73 240 12 755.5 0 12 241 17 755.5 0 17 242 41 755.5 0 41 243 57 755.5 0 57 244 39 755.5 0 39 245 39 755.5 0 39 246 32 755.5 0 32 247 30 755.5 0 30 248 36 755.5 0 36 249 24 755.5 0 24 250 9 755.5 0 9 251 21 755.5 0 21 252 33 755.5 0 33 253 52 755.5 0 52 254 83 755.5 0 83 255 145 755.5 0 145 256 694 755.5 0 694 257 83 755.5 0 83 258 51 755.5 0 51 259 27 755.5 0 27 260 11 755.5 0 11 261 25 755.5 0 25 262 124 755.5 0 124 263 580 755.5 0 580 264 481 755.5 0 481 265 44 755.5 0 44 266 44 755.5 0 44 267 47 755.5 0 47 268 28 755.5 0 28 269 52 755.5 0 52 270 27 755.5 0 27 271 11 755.5 0 11 272 18 755.5 0 18 273 21 755.5 0 21 274 20 755.5 0 20 275 32 755.5 0 32 276 20 755.5 0 20 277 11 755.5 0 11 278 16 755.5 0 16 279 32 755.5 0 32 280 46 755.5 0 46 281 41 755.5 0 41 282 16 755.5 0 16 283 16 755.5 0 16 284 12 755.5 0 12 285 32 755.5 0 32 286 33 755.5 0 33 287 53 755.5 0 53 288 21 755.5 0 21 289 16 755.5 0 16 290 6 755.5 0 6 291 17 755.5 0 17 292 21 755.5 0 21 293 19 755.5 0 19 294 16 755.5 0 16 295 17 755.5 0 17 296 4 755.5 0 4 297 10 755.5 0 10 298 4 755.5 0 4 299 8 755.5 0 8 300 10 755.5 0 10 301 30 755.5 0 30 Finished in 1.07 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 24,211 Reads with adapters: 18,445 (76.2%) Reads that were too short: 2 (0.0%) Reads written (passing filters): 18,443 (76.2%) Total basepairs processed: 6,396,224 bp Total written (filtered): 5,089,978 bp (79.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 18445 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 5 0.0 3 0 0 4 1 16 140 0.0 3 0 132 8 17 18106 0.0 3 17867 226 9 4 18 52 0.0 3 5 42 5 19 39 0.0 3 1 0 35 3 20 96 0.0 3 3 0 0 93 82 1 0.0 3 1 93 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 110 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.80 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 18,443 Reads with adapters: 1,164 (6.3%) Reads written (passing filters): 18,443 (100.0%) Total basepairs processed: 5,089,978 bp Total written (filtered): 5,086,470 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1164 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 11.8% C: 1.7% G: 85.7% T: 0.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1148 288.2 0 1148 4 16 72.0 0 16 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.