This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AGCCTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 94.82 s (31 us/read; 1.96 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 73,005 (2.4%) Reads that were too short: 4,454 (0.1%) Reads written (passing filters): 68,551 (2.2%) Total basepairs processed: 931,490,553 bp Total written (filtered): 15,756,970 bp (1.7%) === Adapter 1 === Sequence: AGCCTC; Type: regular 5'; Length: 6; Trimmed: 73005 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 294 755.5 0 294 7 3081 755.5 0 3081 8 10679 755.5 0 10679 9 25 755.5 0 25 10 19 755.5 0 19 11 29 755.5 0 29 12 38 755.5 0 38 13 19 755.5 0 19 14 12 755.5 0 12 15 17 755.5 0 17 16 26 755.5 0 26 17 16 755.5 0 16 18 20 755.5 0 20 19 24 755.5 0 24 20 30 755.5 0 30 21 18 755.5 0 18 22 23 755.5 0 23 23 21 755.5 0 21 24 23 755.5 0 23 25 15 755.5 0 15 26 24 755.5 0 24 27 18 755.5 0 18 28 23 755.5 0 23 29 70 755.5 0 70 30 21 755.5 0 21 31 12 755.5 0 12 32 24 755.5 0 24 33 15 755.5 0 15 34 17 755.5 0 17 35 18 755.5 0 18 36 24 755.5 0 24 37 25 755.5 0 25 38 47 755.5 0 47 39 164 755.5 0 164 40 37 755.5 0 37 41 48 755.5 0 48 42 32 755.5 0 32 43 32 755.5 0 32 44 87 755.5 0 87 45 65 755.5 0 65 46 73 755.5 0 73 47 38 755.5 0 38 48 41 755.5 0 41 49 87 755.5 0 87 50 90 755.5 0 90 51 172 755.5 0 172 52 216 755.5 0 216 53 262 755.5 0 262 54 574 755.5 0 574 55 1358 755.5 0 1358 56 19523 755.5 0 19523 57 5186 755.5 0 5186 58 10730 755.5 0 10730 59 64 755.5 0 64 60 18 755.5 0 18 61 35 755.5 0 35 62 47 755.5 0 47 63 19 755.5 0 19 64 18 755.5 0 18 65 12 755.5 0 12 66 15 755.5 0 15 67 11 755.5 0 11 68 15 755.5 0 15 69 9 755.5 0 9 70 21 755.5 0 21 71 12 755.5 0 12 72 8 755.5 0 8 73 13 755.5 0 13 74 22 755.5 0 22 75 10 755.5 0 10 76 16 755.5 0 16 77 13 755.5 0 13 78 9 755.5 0 9 79 25 755.5 0 25 80 18 755.5 0 18 81 19 755.5 0 19 82 21 755.5 0 21 83 76 755.5 0 76 84 35 755.5 0 35 85 40 755.5 0 40 86 19 755.5 0 19 87 21 755.5 0 21 88 28 755.5 0 28 89 239 755.5 0 239 90 22 755.5 0 22 91 6 755.5 0 6 92 8 755.5 0 8 93 16 755.5 0 16 94 26 755.5 0 26 95 18 755.5 0 18 96 10 755.5 0 10 97 17 755.5 0 17 98 8 755.5 0 8 99 13 755.5 0 13 100 14 755.5 0 14 101 26 755.5 0 26 102 14 755.5 0 14 103 32 755.5 0 32 104 35 755.5 0 35 105 116 755.5 0 116 106 16 755.5 0 16 107 15 755.5 0 15 108 11 755.5 0 11 109 10 755.5 0 10 110 21 755.5 0 21 111 17 755.5 0 17 112 12 755.5 0 12 113 17 755.5 0 17 114 22 755.5 0 22 115 14 755.5 0 14 116 17 755.5 0 17 117 20 755.5 0 20 118 10 755.5 0 10 119 53 755.5 0 53 120 46 755.5 0 46 121 24 755.5 0 24 122 12 755.5 0 12 123 23 755.5 0 23 124 25 755.5 0 25 125 25 755.5 0 25 126 86 755.5 0 86 127 22 755.5 0 22 128 94 755.5 0 94 129 14 755.5 0 14 130 8 755.5 0 8 131 20 755.5 0 20 132 28 755.5 0 28 133 19 755.5 0 19 134 24 755.5 0 24 135 25 755.5 0 25 136 18 755.5 0 18 137 22 755.5 0 22 138 30 755.5 0 30 139 36 755.5 0 36 140 22 755.5 0 22 141 120 755.5 0 120 142 192 755.5 0 192 143 17 755.5 0 17 144 23 755.5 0 23 145 40 755.5 0 40 146 55 755.5 0 55 147 529 755.5 0 529 148 208 755.5 0 208 149 75 755.5 0 75 150 158 755.5 0 158 151 44 755.5 0 44 152 174 755.5 0 174 153 22 755.5 0 22 154 15 755.5 0 15 155 16 755.5 0 16 156 36 755.5 0 36 157 37 755.5 0 37 158 36 755.5 0 36 159 34 755.5 0 34 160 65 755.5 0 65 161 33 755.5 0 33 162 48 755.5 0 48 163 32 755.5 0 32 164 42 755.5 0 42 165 75 755.5 0 75 166 126 755.5 0 126 167 1047 755.5 0 1047 168 489 755.5 0 489 169 420 755.5 0 420 170 59 755.5 0 59 171 112 755.5 0 112 172 149 755.5 0 149 173 141 755.5 0 141 174 86 755.5 0 86 175 119 755.5 0 119 176 61 755.5 0 61 177 131 755.5 0 131 178 98 755.5 0 98 179 35 755.5 0 35 180 54 755.5 0 54 181 53 755.5 0 53 182 47 755.5 0 47 183 62 755.5 0 62 184 39 755.5 0 39 185 36 755.5 0 36 186 35 755.5 0 35 187 20 755.5 0 20 188 29 755.5 0 29 189 33 755.5 0 33 190 39 755.5 0 39 191 51 755.5 0 51 192 86 755.5 0 86 193 74 755.5 0 74 194 56 755.5 0 56 195 91 755.5 0 91 196 240 755.5 0 240 197 370 755.5 0 370 198 175 755.5 0 175 199 444 755.5 0 444 200 4980 755.5 0 4980 201 569 755.5 0 569 202 1145 755.5 0 1145 203 25 755.5 0 25 204 16 755.5 0 16 205 30 755.5 0 30 206 23 755.5 0 23 207 33 755.5 0 33 208 16 755.5 0 16 209 7 755.5 0 7 210 16 755.5 0 16 211 10 755.5 0 10 212 21 755.5 0 21 213 20 755.5 0 20 214 24 755.5 0 24 215 25 755.5 0 25 216 27 755.5 0 27 217 38 755.5 0 38 218 23 755.5 0 23 219 81 755.5 0 81 220 23 755.5 0 23 221 26 755.5 0 26 222 18 755.5 0 18 223 24 755.5 0 24 224 66 755.5 0 66 225 42 755.5 0 42 226 27 755.5 0 27 227 30 755.5 0 30 228 50 755.5 0 50 229 101 755.5 0 101 230 54 755.5 0 54 231 74 755.5 0 74 232 109 755.5 0 109 233 48 755.5 0 48 234 55 755.5 0 55 235 20 755.5 0 20 236 13 755.5 0 13 237 12 755.5 0 12 238 15 755.5 0 15 239 12 755.5 0 12 240 7 755.5 0 7 241 14 755.5 0 14 242 14 755.5 0 14 243 30 755.5 0 30 244 36 755.5 0 36 245 27 755.5 0 27 246 21 755.5 0 21 247 12 755.5 0 12 248 18 755.5 0 18 249 11 755.5 0 11 250 13 755.5 0 13 251 31 755.5 0 31 252 41 755.5 0 41 253 49 755.5 0 49 254 36 755.5 0 36 255 36 755.5 0 36 256 23 755.5 0 23 257 29 755.5 0 29 258 20 755.5 0 20 259 7 755.5 0 7 260 11 755.5 0 11 261 27 755.5 0 27 262 29 755.5 0 29 263 30 755.5 0 30 264 51 755.5 0 51 265 34 755.5 0 34 266 33 755.5 0 33 267 16 755.5 0 16 268 14 755.5 0 14 269 21 755.5 0 21 270 12 755.5 0 12 271 27 755.5 0 27 272 49 755.5 0 49 273 101 755.5 0 101 274 35 755.5 0 35 275 30 755.5 0 30 276 26 755.5 0 26 277 38 755.5 0 38 278 33 755.5 0 33 279 81 755.5 0 81 280 229 755.5 0 229 281 31 755.5 0 31 282 34 755.5 0 34 283 45 755.5 0 45 284 27 755.5 0 27 285 14 755.5 0 14 286 13 755.5 0 13 287 14 755.5 0 14 288 21 755.5 0 21 289 19 755.5 0 19 290 27 755.5 0 27 291 39 755.5 0 39 292 155 755.5 0 155 293 70 755.5 0 70 294 24 755.5 0 24 295 15 755.5 0 15 296 9 755.5 0 9 297 5 755.5 0 5 298 11 755.5 0 11 299 26 755.5 0 26 300 31 755.5 0 31 301 23 755.5 0 23 Finished in 3.02 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 68,551 Reads with adapters: 2,704 (3.9%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 2,692 (3.9%) Total basepairs processed: 15,756,970 bp Total written (filtered): 743,515 bp (4.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 2704 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 17 0.0 3 0 17 17 2644 0.0 3 2600 44 18 3 0.0 3 0 2 1 19 3 0.0 3 0 0 0 3 20 11 0.0 3 0 0 0 11 21 1 0.0 3 1 22 1 0.0 3 0 0 0 1 24 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 51 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 97 1 0.0 3 0 0 0 1 143 2 0.0 3 0 0 0 2 163 1 0.0 3 0 0 0 1 192 4 0.0 3 0 0 0 4 202 1 0.0 3 0 0 0 1 221 2 0.0 3 0 0 0 2 232 5 0.0 3 0 0 0 5 238 1 0.0 3 0 0 0 1 281 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.12 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 2,692 Reads with adapters: 203 (7.5%) Reads written (passing filters): 2,692 (100.0%) Total basepairs processed: 743,515 bp Total written (filtered): 742,887 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 203 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 52.7% C: 23.6% G: 12.3% T: 11.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 188 42.1 0 188 4 13 10.5 0 13 6 2 0.7 0 2