This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGAATC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 92.12 s (30 us/read; 2.02 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 87,507 (2.8%) Reads that were too short: 14,761 (0.5%) Reads written (passing filters): 72,746 (2.4%) Total basepairs processed: 931,490,553 bp Total written (filtered): 18,351,705 bp (2.0%) === Adapter 1 === Sequence: CGAATC; Type: regular 5'; Length: 6; Trimmed: 87507 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1348 755.5 0 1348 7 39726 755.5 0 39726 8 38 755.5 0 38 9 43 755.5 0 43 10 4 755.5 0 4 11 15 755.5 0 15 12 4 755.5 0 4 13 4 755.5 0 4 14 12 755.5 0 12 15 10 755.5 0 10 16 7 755.5 0 7 17 9 755.5 0 9 18 8 755.5 0 8 19 8 755.5 0 8 20 13 755.5 0 13 21 7 755.5 0 7 22 6 755.5 0 6 23 8 755.5 0 8 24 14 755.5 0 14 25 6 755.5 0 6 26 4 755.5 0 4 27 25 755.5 0 25 28 54 755.5 0 54 29 38 755.5 0 38 30 66 755.5 0 66 31 7 755.5 0 7 32 10 755.5 0 10 33 22 755.5 0 22 34 85 755.5 0 85 35 161 755.5 0 161 36 13 755.5 0 13 37 22 755.5 0 22 38 7 755.5 0 7 39 8 755.5 0 8 40 9 755.5 0 9 41 6 755.5 0 6 42 16 755.5 0 16 43 14 755.5 0 14 44 12 755.5 0 12 45 21 755.5 0 21 46 29 755.5 0 29 47 18 755.5 0 18 48 16 755.5 0 16 49 31 755.5 0 31 50 41 755.5 0 41 51 39 755.5 0 39 52 122 755.5 0 122 53 1753 755.5 0 1753 54 5104 755.5 0 5104 55 1631 755.5 0 1631 56 998 755.5 0 998 57 12 755.5 0 12 58 5 755.5 0 5 59 1 755.5 0 1 60 7 755.5 0 7 61 7 755.5 0 7 62 9 755.5 0 9 63 7 755.5 0 7 64 5 755.5 0 5 65 3 755.5 0 3 66 2 755.5 0 2 67 4 755.5 0 4 68 8 755.5 0 8 69 11 755.5 0 11 70 9 755.5 0 9 71 5 755.5 0 5 72 7 755.5 0 7 73 10 755.5 0 10 74 16 755.5 0 16 75 78 755.5 0 78 76 17 755.5 0 17 77 21 755.5 0 21 78 33 755.5 0 33 79 40 755.5 0 40 80 36 755.5 0 36 81 122 755.5 0 122 82 62 755.5 0 62 83 92 755.5 0 92 84 86 755.5 0 86 85 60 755.5 0 60 86 55 755.5 0 55 87 131 755.5 0 131 88 238 755.5 0 238 89 38 755.5 0 38 90 73 755.5 0 73 91 165 755.5 0 165 92 132 755.5 0 132 93 148 755.5 0 148 94 1346 755.5 0 1346 95 1070 755.5 0 1070 96 5013 755.5 0 5013 97 108 755.5 0 108 98 104 755.5 0 104 99 41 755.5 0 41 100 10 755.5 0 10 101 11 755.5 0 11 102 27 755.5 0 27 103 26 755.5 0 26 104 32 755.5 0 32 105 18 755.5 0 18 106 4 755.5 0 4 107 18 755.5 0 18 108 112 755.5 0 112 109 64 755.5 0 64 110 21 755.5 0 21 111 79 755.5 0 79 112 26 755.5 0 26 113 45 755.5 0 45 114 40 755.5 0 40 115 28 755.5 0 28 116 95 755.5 0 95 117 315 755.5 0 315 118 74 755.5 0 74 119 217 755.5 0 217 120 88 755.5 0 88 121 316 755.5 0 316 122 169 755.5 0 169 123 386 755.5 0 386 124 68 755.5 0 68 125 41 755.5 0 41 126 68 755.5 0 68 127 36 755.5 0 36 128 79 755.5 0 79 129 122 755.5 0 122 130 81 755.5 0 81 131 46 755.5 0 46 132 45 755.5 0 45 133 54 755.5 0 54 134 67 755.5 0 67 135 85 755.5 0 85 136 75 755.5 0 75 137 78 755.5 0 78 138 63 755.5 0 63 139 203 755.5 0 203 140 102 755.5 0 102 141 322 755.5 0 322 142 87 755.5 0 87 143 88 755.5 0 88 144 44 755.5 0 44 145 62 755.5 0 62 146 84 755.5 0 84 147 161 755.5 0 161 148 105 755.5 0 105 149 76 755.5 0 76 150 118 755.5 0 118 151 113 755.5 0 113 152 86 755.5 0 86 153 52 755.5 0 52 154 42 755.5 0 42 155 110 755.5 0 110 156 119 755.5 0 119 157 87 755.5 0 87 158 78 755.5 0 78 159 85 755.5 0 85 160 140 755.5 0 140 161 109 755.5 0 109 162 170 755.5 0 170 163 182 755.5 0 182 164 80 755.5 0 80 165 107 755.5 0 107 166 91 755.5 0 91 167 199 755.5 0 199 168 133 755.5 0 133 169 71 755.5 0 71 170 55 755.5 0 55 171 104 755.5 0 104 172 178 755.5 0 178 173 69 755.5 0 69 174 36 755.5 0 36 175 150 755.5 0 150 176 1331 755.5 0 1331 177 396 755.5 0 396 178 468 755.5 0 468 179 51 755.5 0 51 180 69 755.5 0 69 181 120 755.5 0 120 182 126 755.5 0 126 183 92 755.5 0 92 184 98 755.5 0 98 185 62 755.5 0 62 186 62 755.5 0 62 187 77 755.5 0 77 188 199 755.5 0 199 189 108 755.5 0 108 190 60 755.5 0 60 191 57 755.5 0 57 192 108 755.5 0 108 193 97 755.5 0 97 194 82 755.5 0 82 195 109 755.5 0 109 196 193 755.5 0 193 197 57 755.5 0 57 198 25 755.5 0 25 199 26 755.5 0 26 200 81 755.5 0 81 201 156 755.5 0 156 202 98 755.5 0 98 203 34 755.5 0 34 204 60 755.5 0 60 205 107 755.5 0 107 206 61 755.5 0 61 207 151 755.5 0 151 208 109 755.5 0 109 209 91 755.5 0 91 210 39 755.5 0 39 211 53 755.5 0 53 212 124 755.5 0 124 213 130 755.5 0 130 214 84 755.5 0 84 215 115 755.5 0 115 216 132 755.5 0 132 217 68 755.5 0 68 218 63 755.5 0 63 219 75 755.5 0 75 220 190 755.5 0 190 221 329 755.5 0 329 222 168 755.5 0 168 223 77 755.5 0 77 224 64 755.5 0 64 225 86 755.5 0 86 226 115 755.5 0 115 227 95 755.5 0 95 228 117 755.5 0 117 229 103 755.5 0 103 230 83 755.5 0 83 231 87 755.5 0 87 232 210 755.5 0 210 233 162 755.5 0 162 234 77 755.5 0 77 235 42 755.5 0 42 236 75 755.5 0 75 237 137 755.5 0 137 238 102 755.5 0 102 239 85 755.5 0 85 240 93 755.5 0 93 241 99 755.5 0 99 242 135 755.5 0 135 243 66 755.5 0 66 244 87 755.5 0 87 245 72 755.5 0 72 246 59 755.5 0 59 247 102 755.5 0 102 248 227 755.5 0 227 249 231 755.5 0 231 250 179 755.5 0 179 251 165 755.5 0 165 252 105 755.5 0 105 253 75 755.5 0 75 254 87 755.5 0 87 255 84 755.5 0 84 256 123 755.5 0 123 257 133 755.5 0 133 258 153 755.5 0 153 259 152 755.5 0 152 260 114 755.5 0 114 261 124 755.5 0 124 262 590 755.5 0 590 263 301 755.5 0 301 264 206 755.5 0 206 265 94 755.5 0 94 266 89 755.5 0 89 267 70 755.5 0 70 268 90 755.5 0 90 269 226 755.5 0 226 270 146 755.5 0 146 271 142 755.5 0 142 272 130 755.5 0 130 273 122 755.5 0 122 274 81 755.5 0 81 275 112 755.5 0 112 276 217 755.5 0 217 277 124 755.5 0 124 278 115 755.5 0 115 279 88 755.5 0 88 280 655 755.5 0 655 281 215 755.5 0 215 282 643 755.5 0 643 283 352 755.5 0 352 284 183 755.5 0 183 285 46 755.5 0 46 286 117 755.5 0 117 287 175 755.5 0 175 288 240 755.5 0 240 289 846 755.5 0 846 290 158 755.5 0 158 291 216 755.5 0 216 292 194 755.5 0 194 293 132 755.5 0 132 294 109 755.5 0 109 295 169 755.5 0 169 296 153 755.5 0 153 297 81 755.5 0 81 298 223 755.5 0 223 299 136 755.5 0 136 300 76 755.5 0 76 301 36 755.5 0 36 Finished in 3.42 s (47 us/read; 1.28 M reads/minute). === Summary === Total reads processed: 72,746 Reads with adapters: 41,048 (56.4%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 41,039 (56.4%) Total basepairs processed: 18,351,705 bp Total written (filtered): 11,245,384 bp (61.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 41048 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 2 18 11 0.0 3 0 0 8 2 1 19 394 0.0 3 0 374 10 7 3 20 40514 0.0 4 39592 793 88 31 10 21 81 0.0 4 7 72 0 2 22 7 0.0 4 3 0 3 0 1 23 21 0.0 4 0 0 0 17 4 24 1 0.0 4 0 0 0 1 25 1 0.0 4 1 36 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 46 2 0.0 4 0 0 0 0 2 51 1 0.0 4 0 0 0 1 54 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.70 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 41,039 Reads with adapters: 1,569 (3.8%) Reads written (passing filters): 41,039 (100.0%) Total basepairs processed: 11,245,384 bp Total written (filtered): 11,225,908 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1569 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.7% C: 2.8% G: 91.6% T: 1.9% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 12 641.2 0 12 4 4 160.3 0 4 5 2 40.1 0 2 9 1 0.2 0 1 10 7 0.0 1 0 7 11 1430 0.0 1 4 1426 12 102 0.0 1 2 100 14 1 0.0 1 0 1 210 5 0.0 1 5 260 1 0.0 1 1 261 1 0.0 1 1 262 1 0.0 1 1 265 1 0.0 1 1 269 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.