This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTCAAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 91.04 s (29 us/read; 2.04 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 109,071 (3.5%) Reads that were too short: 12,490 (0.4%) Reads written (passing filters): 96,581 (3.1%) Total basepairs processed: 931,490,553 bp Total written (filtered): 24,789,987 bp (2.7%) === Adapter 1 === Sequence: GTCAAT; Type: regular 5'; Length: 6; Trimmed: 109071 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1128 755.5 0 1128 7 356 755.5 0 356 8 768 755.5 0 768 9 38789 755.5 0 38789 10 60 755.5 0 60 11 8 755.5 0 8 12 13 755.5 0 13 13 18 755.5 0 18 14 16 755.5 0 16 15 17 755.5 0 17 16 30 755.5 0 30 17 24 755.5 0 24 18 18 755.5 0 18 19 13 755.5 0 13 20 18 755.5 0 18 21 16 755.5 0 16 22 16 755.5 0 16 23 24 755.5 0 24 24 17 755.5 0 17 25 23 755.5 0 23 26 26 755.5 0 26 27 39 755.5 0 39 28 54 755.5 0 54 29 115 755.5 0 115 30 36 755.5 0 36 31 52 755.5 0 52 32 133 755.5 0 133 33 1134 755.5 0 1134 34 175 755.5 0 175 35 51 755.5 0 51 36 71 755.5 0 71 37 90 755.5 0 90 38 97 755.5 0 97 39 80 755.5 0 80 40 82 755.5 0 82 41 127 755.5 0 127 42 1672 755.5 0 1672 43 3745 755.5 0 3745 44 6338 755.5 0 6338 45 41 755.5 0 41 46 18 755.5 0 18 47 33 755.5 0 33 48 127 755.5 0 127 49 38 755.5 0 38 50 29 755.5 0 29 51 36 755.5 0 36 52 37 755.5 0 37 53 76 755.5 0 76 54 65 755.5 0 65 55 51 755.5 0 51 56 31 755.5 0 31 57 49 755.5 0 49 58 100 755.5 0 100 59 76 755.5 0 76 60 128 755.5 0 128 61 187 755.5 0 187 62 224 755.5 0 224 63 383 755.5 0 383 64 957 755.5 0 957 65 13763 755.5 0 13763 66 5653 755.5 0 5653 67 10609 755.5 0 10609 68 55 755.5 0 55 69 23 755.5 0 23 70 14 755.5 0 14 71 42 755.5 0 42 72 17 755.5 0 17 73 15 755.5 0 15 74 13 755.5 0 13 75 11 755.5 0 11 76 8 755.5 0 8 77 9 755.5 0 9 78 15 755.5 0 15 79 13 755.5 0 13 80 18 755.5 0 18 81 34 755.5 0 34 82 50 755.5 0 50 83 20 755.5 0 20 84 23 755.5 0 23 85 55 755.5 0 55 86 23 755.5 0 23 87 90 755.5 0 90 88 24 755.5 0 24 89 15 755.5 0 15 90 35 755.5 0 35 91 41 755.5 0 41 92 25 755.5 0 25 93 35 755.5 0 35 94 22 755.5 0 22 95 26 755.5 0 26 96 92 755.5 0 92 97 596 755.5 0 596 98 39 755.5 0 39 99 18 755.5 0 18 100 30 755.5 0 30 101 54 755.5 0 54 102 151 755.5 0 151 103 137 755.5 0 137 104 75 755.5 0 75 105 23 755.5 0 23 106 30 755.5 0 30 107 41 755.5 0 41 108 54 755.5 0 54 109 16 755.5 0 16 110 17 755.5 0 17 111 27 755.5 0 27 112 67 755.5 0 67 113 41 755.5 0 41 114 22 755.5 0 22 115 33 755.5 0 33 116 18 755.5 0 18 117 27 755.5 0 27 118 35 755.5 0 35 119 71 755.5 0 71 120 64 755.5 0 64 121 73 755.5 0 73 122 68 755.5 0 68 123 67 755.5 0 67 124 54 755.5 0 54 125 50 755.5 0 50 126 46 755.5 0 46 127 73 755.5 0 73 128 98 755.5 0 98 129 42 755.5 0 42 130 29 755.5 0 29 131 34 755.5 0 34 132 58 755.5 0 58 133 156 755.5 0 156 134 154 755.5 0 154 135 76 755.5 0 76 136 95 755.5 0 95 137 101 755.5 0 101 138 240 755.5 0 240 139 160 755.5 0 160 140 102 755.5 0 102 141 131 755.5 0 131 142 93 755.5 0 93 143 54 755.5 0 54 144 36 755.5 0 36 145 33 755.5 0 33 146 55 755.5 0 55 147 73 755.5 0 73 148 52 755.5 0 52 149 62 755.5 0 62 150 69 755.5 0 69 151 34 755.5 0 34 152 24 755.5 0 24 153 39 755.5 0 39 154 33 755.5 0 33 155 54 755.5 0 54 156 47 755.5 0 47 157 39 755.5 0 39 158 54 755.5 0 54 159 158 755.5 0 158 160 83 755.5 0 83 161 83 755.5 0 83 162 65 755.5 0 65 163 52 755.5 0 52 164 57 755.5 0 57 165 55 755.5 0 55 166 73 755.5 0 73 167 71 755.5 0 71 168 82 755.5 0 82 169 39 755.5 0 39 170 92 755.5 0 92 171 108 755.5 0 108 172 88 755.5 0 88 173 43 755.5 0 43 174 75 755.5 0 75 175 60 755.5 0 60 176 64 755.5 0 64 177 49 755.5 0 49 178 29 755.5 0 29 179 35 755.5 0 35 180 40 755.5 0 40 181 70 755.5 0 70 182 91 755.5 0 91 183 66 755.5 0 66 184 99 755.5 0 99 185 92 755.5 0 92 186 141 755.5 0 141 187 105 755.5 0 105 188 75 755.5 0 75 189 45 755.5 0 45 190 35 755.5 0 35 191 37 755.5 0 37 192 63 755.5 0 63 193 46 755.5 0 46 194 72 755.5 0 72 195 92 755.5 0 92 196 42 755.5 0 42 197 31 755.5 0 31 198 28 755.5 0 28 199 63 755.5 0 63 200 177 755.5 0 177 201 103 755.5 0 103 202 33 755.5 0 33 203 37 755.5 0 37 204 41 755.5 0 41 205 63 755.5 0 63 206 164 755.5 0 164 207 246 755.5 0 246 208 142 755.5 0 142 209 75 755.5 0 75 210 55 755.5 0 55 211 79 755.5 0 79 212 82 755.5 0 82 213 51 755.5 0 51 214 103 755.5 0 103 215 139 755.5 0 139 216 146 755.5 0 146 217 253 755.5 0 253 218 123 755.5 0 123 219 144 755.5 0 144 220 194 755.5 0 194 221 113 755.5 0 113 222 55 755.5 0 55 223 79 755.5 0 79 224 158 755.5 0 158 225 158 755.5 0 158 226 110 755.5 0 110 227 117 755.5 0 117 228 100 755.5 0 100 229 98 755.5 0 98 230 112 755.5 0 112 231 191 755.5 0 191 232 97 755.5 0 97 233 49 755.5 0 49 234 30 755.5 0 30 235 59 755.5 0 59 236 127 755.5 0 127 237 152 755.5 0 152 238 108 755.5 0 108 239 114 755.5 0 114 240 128 755.5 0 128 241 88 755.5 0 88 242 54 755.5 0 54 243 63 755.5 0 63 244 125 755.5 0 125 245 141 755.5 0 141 246 124 755.5 0 124 247 127 755.5 0 127 248 111 755.5 0 111 249 177 755.5 0 177 250 59 755.5 0 59 251 34 755.5 0 34 252 24 755.5 0 24 253 55 755.5 0 55 254 132 755.5 0 132 255 778 755.5 0 778 256 540 755.5 0 540 257 430 755.5 0 430 258 283 755.5 0 283 259 207 755.5 0 207 260 118 755.5 0 118 261 56 755.5 0 56 262 47 755.5 0 47 263 53 755.5 0 53 264 104 755.5 0 104 265 133 755.5 0 133 266 139 755.5 0 139 267 162 755.5 0 162 268 193 755.5 0 193 269 171 755.5 0 171 270 93 755.5 0 93 271 73 755.5 0 73 272 46 755.5 0 46 273 39 755.5 0 39 274 262 755.5 0 262 275 246 755.5 0 246 276 186 755.5 0 186 277 63 755.5 0 63 278 58 755.5 0 58 279 64 755.5 0 64 280 135 755.5 0 135 281 150 755.5 0 150 282 91 755.5 0 91 283 52 755.5 0 52 284 46 755.5 0 46 285 78 755.5 0 78 286 82 755.5 0 82 287 105 755.5 0 105 288 163 755.5 0 163 289 145 755.5 0 145 290 92 755.5 0 92 291 42 755.5 0 42 292 25 755.5 0 25 293 174 755.5 0 174 294 261 755.5 0 261 295 189 755.5 0 189 296 106 755.5 0 106 297 77 755.5 0 77 298 59 755.5 0 59 299 48 755.5 0 48 300 57 755.5 0 57 301 130 755.5 0 130 Finished in 4.29 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 96,581 Reads with adapters: 39,964 (41.4%) Reads that were too short: 5 (0.0%) Reads written (passing filters): 39,959 (41.4%) Total basepairs processed: 24,789,987 bp Total written (filtered): 10,989,808 bp (44.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 39964 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 10 0.0 3 0 0 10 16 320 0.0 3 0 313 6 1 17 39471 0.0 3 38596 847 26 2 18 92 0.0 3 8 77 7 19 26 0.0 3 0 0 22 4 20 30 0.0 3 0 0 0 30 21 3 0.0 3 3 23 1 0.0 3 0 0 1 37 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 160 1 0.0 3 0 0 0 1 200 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 286 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.69 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 39,959 Reads with adapters: 3,528 (8.8%) Reads written (passing filters): 39,959 (100.0%) Total basepairs processed: 10,989,808 bp Total written (filtered): 10,979,199 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 3528 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 89.9% C: 2.2% G: 7.5% T: 0.4% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3504 624.4 0 3504 4 23 156.1 0 23 5 1 39.0 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.