This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 93.72 s (30 us/read; 1.98 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 269,445 (8.7%) Reads that were too short: 90,631 (2.9%) Reads written (passing filters): 178,814 (5.8%) Total basepairs processed: 931,490,553 bp Total written (filtered): 36,586,329 bp (3.9%) === Adapter 1 === Sequence: AATGAA; Type: regular 5'; Length: 6; Trimmed: 269445 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 351 755.5 0 351 7 464 755.5 0 464 8 1352 755.5 0 1352 9 49586 755.5 0 49586 10 86 755.5 0 86 11 13 755.5 0 13 12 8 755.5 0 8 13 8 755.5 0 8 14 9 755.5 0 9 15 12 755.5 0 12 16 5 755.5 0 5 17 5 755.5 0 5 18 10 755.5 0 10 19 8 755.5 0 8 20 5 755.5 0 5 21 10 755.5 0 10 22 8 755.5 0 8 23 14 755.5 0 14 24 12 755.5 0 12 25 11 755.5 0 11 26 9 755.5 0 9 27 8 755.5 0 8 28 7 755.5 0 7 29 11 755.5 0 11 30 28 755.5 0 28 31 8 755.5 0 8 32 21 755.5 0 21 33 7 755.5 0 7 34 6 755.5 0 6 35 6 755.5 0 6 36 6 755.5 0 6 37 23 755.5 0 23 38 15 755.5 0 15 39 14 755.5 0 14 40 9 755.5 0 9 41 7 755.5 0 7 42 12 755.5 0 12 43 44 755.5 0 44 44 11 755.5 0 11 45 10 755.5 0 10 46 23 755.5 0 23 47 63 755.5 0 63 48 8 755.5 0 8 49 11 755.5 0 11 50 11 755.5 0 11 51 11 755.5 0 11 52 6 755.5 0 6 53 9 755.5 0 9 54 6 755.5 0 6 55 8 755.5 0 8 56 5 755.5 0 5 57 8 755.5 0 8 58 38 755.5 0 38 59 15 755.5 0 15 60 7 755.5 0 7 61 28 755.5 0 28 62 55 755.5 0 55 63 18 755.5 0 18 64 16 755.5 0 16 65 20 755.5 0 20 66 16 755.5 0 16 67 11 755.5 0 11 68 19 755.5 0 19 69 31 755.5 0 31 70 72 755.5 0 72 71 82 755.5 0 82 72 703 755.5 0 703 73 476 755.5 0 476 74 171 755.5 0 171 75 197 755.5 0 197 76 557 755.5 0 557 77 1999 755.5 0 1999 78 900 755.5 0 900 79 1775 755.5 0 1775 80 2002 755.5 0 2002 81 3006 755.5 0 3006 82 1470 755.5 0 1470 83 1556 755.5 0 1556 84 3140 755.5 0 3140 85 2260 755.5 0 2260 86 1816 755.5 0 1816 87 368 755.5 0 368 88 274 755.5 0 274 89 818 755.5 0 818 90 796 755.5 0 796 91 284 755.5 0 284 92 348 755.5 0 348 93 1038 755.5 0 1038 94 1631 755.5 0 1631 95 2554 755.5 0 2554 96 1250 755.5 0 1250 97 300 755.5 0 300 98 291 755.5 0 291 99 1163 755.5 0 1163 100 1960 755.5 0 1960 101 1213 755.5 0 1213 102 487 755.5 0 487 103 417 755.5 0 417 104 167 755.5 0 167 105 450 755.5 0 450 106 1491 755.5 0 1491 107 350 755.5 0 350 108 130 755.5 0 130 109 373 755.5 0 373 110 853 755.5 0 853 111 852 755.5 0 852 112 800 755.5 0 800 113 741 755.5 0 741 114 740 755.5 0 740 115 1337 755.5 0 1337 116 798 755.5 0 798 117 771 755.5 0 771 118 1782 755.5 0 1782 119 1353 755.5 0 1353 120 1008 755.5 0 1008 121 697 755.5 0 697 122 674 755.5 0 674 123 654 755.5 0 654 124 958 755.5 0 958 125 1219 755.5 0 1219 126 2012 755.5 0 2012 127 2025 755.5 0 2025 128 694 755.5 0 694 129 571 755.5 0 571 130 988 755.5 0 988 131 1562 755.5 0 1562 132 1118 755.5 0 1118 133 754 755.5 0 754 134 773 755.5 0 773 135 836 755.5 0 836 136 1239 755.5 0 1239 137 1340 755.5 0 1340 138 1154 755.5 0 1154 139 1162 755.5 0 1162 140 1020 755.5 0 1020 141 1914 755.5 0 1914 142 789 755.5 0 789 143 1736 755.5 0 1736 144 836 755.5 0 836 145 1396 755.5 0 1396 146 932 755.5 0 932 147 709 755.5 0 709 148 821 755.5 0 821 149 843 755.5 0 843 150 635 755.5 0 635 151 532 755.5 0 532 152 823 755.5 0 823 153 1084 755.5 0 1084 154 1122 755.5 0 1122 155 568 755.5 0 568 156 591 755.5 0 591 157 963 755.5 0 963 158 926 755.5 0 926 159 1124 755.5 0 1124 160 788 755.5 0 788 161 536 755.5 0 536 162 730 755.5 0 730 163 650 755.5 0 650 164 699 755.5 0 699 165 711 755.5 0 711 166 735 755.5 0 735 167 589 755.5 0 589 168 723 755.5 0 723 169 922 755.5 0 922 170 916 755.5 0 916 171 484 755.5 0 484 172 411 755.5 0 411 173 928 755.5 0 928 174 1290 755.5 0 1290 175 618 755.5 0 618 176 386 755.5 0 386 177 411 755.5 0 411 178 580 755.5 0 580 179 945 755.5 0 945 180 1073 755.5 0 1073 181 850 755.5 0 850 182 2067 755.5 0 2067 183 959 755.5 0 959 184 1242 755.5 0 1242 185 996 755.5 0 996 186 963 755.5 0 963 187 780 755.5 0 780 188 420 755.5 0 420 189 377 755.5 0 377 190 673 755.5 0 673 191 1176 755.5 0 1176 192 1084 755.5 0 1084 193 1119 755.5 0 1119 194 836 755.5 0 836 195 445 755.5 0 445 196 323 755.5 0 323 197 825 755.5 0 825 198 2421 755.5 0 2421 199 1607 755.5 0 1607 200 477 755.5 0 477 201 203 755.5 0 203 202 474 755.5 0 474 203 1178 755.5 0 1178 204 1095 755.5 0 1095 205 930 755.5 0 930 206 1089 755.5 0 1089 207 634 755.5 0 634 208 412 755.5 0 412 209 529 755.5 0 529 210 884 755.5 0 884 211 1047 755.5 0 1047 212 730 755.5 0 730 213 720 755.5 0 720 214 739 755.5 0 739 215 573 755.5 0 573 216 534 755.5 0 534 217 723 755.5 0 723 218 879 755.5 0 879 219 914 755.5 0 914 220 596 755.5 0 596 221 322 755.5 0 322 222 393 755.5 0 393 223 774 755.5 0 774 224 698 755.5 0 698 225 558 755.5 0 558 226 612 755.5 0 612 227 799 755.5 0 799 228 655 755.5 0 655 229 574 755.5 0 574 230 729 755.5 0 729 231 802 755.5 0 802 232 433 755.5 0 433 233 517 755.5 0 517 234 1151 755.5 0 1151 235 1479 755.5 0 1479 236 959 755.5 0 959 237 1422 755.5 0 1422 238 1242 755.5 0 1242 239 2403 755.5 0 2403 240 686 755.5 0 686 241 600 755.5 0 600 242 618 755.5 0 618 243 697 755.5 0 697 244 528 755.5 0 528 245 460 755.5 0 460 246 751 755.5 0 751 247 843 755.5 0 843 248 959 755.5 0 959 249 952 755.5 0 952 250 828 755.5 0 828 251 2723 755.5 0 2723 252 868 755.5 0 868 253 796 755.5 0 796 254 846 755.5 0 846 255 773 755.5 0 773 256 445 755.5 0 445 257 633 755.5 0 633 258 515 755.5 0 515 259 494 755.5 0 494 260 667 755.5 0 667 261 774 755.5 0 774 262 609 755.5 0 609 263 406 755.5 0 406 264 529 755.5 0 529 265 709 755.5 0 709 266 675 755.5 0 675 267 939 755.5 0 939 268 635 755.5 0 635 269 456 755.5 0 456 270 499 755.5 0 499 271 804 755.5 0 804 272 951 755.5 0 951 273 1158 755.5 0 1158 274 1233 755.5 0 1233 275 769 755.5 0 769 276 600 755.5 0 600 277 1275 755.5 0 1275 278 1168 755.5 0 1168 279 936 755.5 0 936 280 683 755.5 0 683 281 604 755.5 0 604 282 581 755.5 0 581 283 969 755.5 0 969 284 712 755.5 0 712 285 839 755.5 0 839 286 842 755.5 0 842 287 639 755.5 0 639 288 1987 755.5 0 1987 289 2191 755.5 0 2191 290 3043 755.5 0 3043 291 932 755.5 0 932 292 852 755.5 0 852 293 1057 755.5 0 1057 294 910 755.5 0 910 295 700 755.5 0 700 296 1030 755.5 0 1030 297 1233 755.5 0 1233 298 907 755.5 0 907 299 1066 755.5 0 1066 300 2507 755.5 0 2507 301 3338 755.5 0 3338 Finished in 7.64 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 178,814 Reads with adapters: 51,734 (28.9%) Reads that were too short: 49 (0.0%) Reads written (passing filters): 51,685 (28.9%) Total basepairs processed: 36,586,329 bp Total written (filtered): 14,058,509 bp (38.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 51734 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 20 0.0 3 0 0 16 3 1 19 545 0.0 3 0 524 14 4 3 20 50950 0.0 4 49431 1263 175 54 27 21 99 0.0 4 22 72 3 0 2 22 9 0.0 4 0 0 6 0 3 23 34 0.0 4 0 0 0 26 8 24 3 0.0 4 2 0 0 1 26 2 0.0 4 0 0 0 0 2 27 1 0.0 4 1 28 1 0.0 4 1 31 1 0.0 4 1 34 1 0.0 4 0 0 0 0 1 37 1 0.0 4 0 0 0 0 1 38 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 42 2 0.0 4 1 0 0 0 1 44 1 0.0 4 0 0 0 0 1 46 1 0.0 4 0 0 0 0 1 48 2 0.0 4 1 0 0 0 1 50 1 0.0 4 0 0 0 0 1 54 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 65 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 74 1 0.0 4 0 0 0 0 1 77 2 0.0 4 0 0 0 0 2 78 2 0.0 4 0 0 0 0 2 79 1 0.0 4 0 0 0 0 1 88 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 104 2 0.0 4 0 0 0 0 2 107 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 109 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 123 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 0 0 0 1 129 1 0.0 4 0 0 0 0 1 133 2 0.0 4 0 0 0 0 2 134 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 144 2 0.0 4 0 0 0 0 2 152 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 159 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 171 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 182 3 0.0 4 0 0 0 1 2 186 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 203 1 0.0 4 0 0 0 0 1 210 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.98 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 51,685 Reads with adapters: 2,756 (5.3%) Reads written (passing filters): 51,685 (100.0%) Total basepairs processed: 14,058,509 bp Total written (filtered): 14,026,263 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2756 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.7% C: 2.6% G: 89.5% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 3 807.6 0 3 4 5 201.9 0 5 5 2 50.5 0 2 8 2 0.8 0 2 9 796 0.2 0 49 747 10 875 0.0 1 21 854 11 1036 0.0 1 6 1030 12 9 0.0 1 0 9 13 5 0.0 1 0 5 150 6 0.0 1 3 3 151 1 0.0 1 1 153 1 0.0 1 0 1 207 1 0.0 1 0 1 208 7 0.0 1 5 2 258 1 0.0 1 1 259 1 0.0 1 1 263 3 0.0 1 3 267 1 0.0 1 1 268 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.