This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 92.52 s (30 us/read; 2.01 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 145,328 (4.7%) Reads that were too short: 81,465 (2.6%) Reads written (passing filters): 63,863 (2.1%) Total basepairs processed: 931,490,553 bp Total written (filtered): 10,373,407 bp (1.1%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 145328 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 23 755.5 0 23 7 33 755.5 0 33 8 1109 755.5 0 1109 9 9 755.5 0 9 10 13 755.5 0 13 11 8 755.5 0 8 12 6 755.5 0 6 13 7 755.5 0 7 14 4 755.5 0 4 15 7 755.5 0 7 16 27 755.5 0 27 17 11 755.5 0 11 18 9 755.5 0 9 19 4 755.5 0 4 20 5 755.5 0 5 21 4 755.5 0 4 22 3 755.5 0 3 23 10 755.5 0 10 24 4 755.5 0 4 25 13 755.5 0 13 26 148 755.5 0 148 27 113 755.5 0 113 28 105 755.5 0 105 29 10 755.5 0 10 30 7 755.5 0 7 31 3 755.5 0 3 32 2 755.5 0 2 33 4 755.5 0 4 34 7 755.5 0 7 35 3 755.5 0 3 36 14 755.5 0 14 37 15 755.5 0 15 38 18 755.5 0 18 39 5 755.5 0 5 40 10 755.5 0 10 41 10 755.5 0 10 42 15 755.5 0 15 43 9 755.5 0 9 44 6 755.5 0 6 45 4 755.5 0 4 46 9 755.5 0 9 47 11 755.5 0 11 48 9 755.5 0 9 49 6 755.5 0 6 50 8 755.5 0 8 51 3 755.5 0 3 52 11 755.5 0 11 53 6 755.5 0 6 54 12 755.5 0 12 55 2 755.5 0 2 56 7 755.5 0 7 57 7 755.5 0 7 58 17 755.5 0 17 59 10 755.5 0 10 60 11 755.5 0 11 61 12 755.5 0 12 62 8 755.5 0 8 63 67 755.5 0 67 64 6 755.5 0 6 65 6 755.5 0 6 66 4 755.5 0 4 67 8 755.5 0 8 68 7 755.5 0 7 69 11 755.5 0 11 70 8 755.5 0 8 71 42 755.5 0 42 72 8 755.5 0 8 73 71 755.5 0 71 74 29 755.5 0 29 75 165 755.5 0 165 76 45 755.5 0 45 77 88 755.5 0 88 78 102 755.5 0 102 79 260 755.5 0 260 80 128 755.5 0 128 81 352 755.5 0 352 82 214 755.5 0 214 83 245 755.5 0 245 84 150 755.5 0 150 85 231 755.5 0 231 86 419 755.5 0 419 87 60 755.5 0 60 88 274 755.5 0 274 89 258 755.5 0 258 90 93 755.5 0 93 91 222 755.5 0 222 92 270 755.5 0 270 93 128 755.5 0 128 94 409 755.5 0 409 95 541 755.5 0 541 96 917 755.5 0 917 97 682 755.5 0 682 98 620 755.5 0 620 99 575 755.5 0 575 100 334 755.5 0 334 101 268 755.5 0 268 102 501 755.5 0 501 103 261 755.5 0 261 104 113 755.5 0 113 105 557 755.5 0 557 106 634 755.5 0 634 107 461 755.5 0 461 108 1864 755.5 0 1864 109 1202 755.5 0 1202 110 649 755.5 0 649 111 290 755.5 0 290 112 789 755.5 0 789 113 519 755.5 0 519 114 1864 755.5 0 1864 115 1198 755.5 0 1198 116 463 755.5 0 463 117 358 755.5 0 358 118 205 755.5 0 205 119 135 755.5 0 135 120 137 755.5 0 137 121 286 755.5 0 286 122 647 755.5 0 647 123 414 755.5 0 414 124 638 755.5 0 638 125 194 755.5 0 194 126 352 755.5 0 352 127 457 755.5 0 457 128 381 755.5 0 381 129 726 755.5 0 726 130 647 755.5 0 647 131 622 755.5 0 622 132 545 755.5 0 545 133 327 755.5 0 327 134 350 755.5 0 350 135 449 755.5 0 449 136 708 755.5 0 708 137 895 755.5 0 895 138 517 755.5 0 517 139 404 755.5 0 404 140 402 755.5 0 402 141 822 755.5 0 822 142 685 755.5 0 685 143 410 755.5 0 410 144 273 755.5 0 273 145 268 755.5 0 268 146 389 755.5 0 389 147 485 755.5 0 485 148 1181 755.5 0 1181 149 584 755.5 0 584 150 702 755.5 0 702 151 321 755.5 0 321 152 343 755.5 0 343 153 528 755.5 0 528 154 328 755.5 0 328 155 619 755.5 0 619 156 563 755.5 0 563 157 670 755.5 0 670 158 376 755.5 0 376 159 351 755.5 0 351 160 358 755.5 0 358 161 407 755.5 0 407 162 358 755.5 0 358 163 654 755.5 0 654 164 507 755.5 0 507 165 589 755.5 0 589 166 403 755.5 0 403 167 488 755.5 0 488 168 402 755.5 0 402 169 573 755.5 0 573 170 415 755.5 0 415 171 215 755.5 0 215 172 380 755.5 0 380 173 251 755.5 0 251 174 468 755.5 0 468 175 645 755.5 0 645 176 910 755.5 0 910 177 384 755.5 0 384 178 289 755.5 0 289 179 242 755.5 0 242 180 233 755.5 0 233 181 405 755.5 0 405 182 454 755.5 0 454 183 462 755.5 0 462 184 406 755.5 0 406 185 537 755.5 0 537 186 856 755.5 0 856 187 829 755.5 0 829 188 492 755.5 0 492 189 270 755.5 0 270 190 328 755.5 0 328 191 441 755.5 0 441 192 615 755.5 0 615 193 805 755.5 0 805 194 1286 755.5 0 1286 195 444 755.5 0 444 196 440 755.5 0 440 197 308 755.5 0 308 198 322 755.5 0 322 199 776 755.5 0 776 200 1001 755.5 0 1001 201 873 755.5 0 873 202 865 755.5 0 865 203 295 755.5 0 295 204 326 755.5 0 326 205 553 755.5 0 553 206 678 755.5 0 678 207 450 755.5 0 450 208 1056 755.5 0 1056 209 675 755.5 0 675 210 582 755.5 0 582 211 442 755.5 0 442 212 526 755.5 0 526 213 454 755.5 0 454 214 362 755.5 0 362 215 334 755.5 0 334 216 354 755.5 0 354 217 309 755.5 0 309 218 417 755.5 0 417 219 619 755.5 0 619 220 559 755.5 0 559 221 456 755.5 0 456 222 520 755.5 0 520 223 305 755.5 0 305 224 242 755.5 0 242 225 323 755.5 0 323 226 443 755.5 0 443 227 584 755.5 0 584 228 670 755.5 0 670 229 757 755.5 0 757 230 925 755.5 0 925 231 577 755.5 0 577 232 372 755.5 0 372 233 384 755.5 0 384 234 361 755.5 0 361 235 473 755.5 0 473 236 779 755.5 0 779 237 956 755.5 0 956 238 828 755.5 0 828 239 543 755.5 0 543 240 560 755.5 0 560 241 274 755.5 0 274 242 368 755.5 0 368 243 360 755.5 0 360 244 492 755.5 0 492 245 661 755.5 0 661 246 893 755.5 0 893 247 1313 755.5 0 1313 248 1253 755.5 0 1253 249 819 755.5 0 819 250 348 755.5 0 348 251 192 755.5 0 192 252 131 755.5 0 131 253 232 755.5 0 232 254 286 755.5 0 286 255 349 755.5 0 349 256 879 755.5 0 879 257 1292 755.5 0 1292 258 4144 755.5 0 4144 259 2507 755.5 0 2507 260 3870 755.5 0 3870 261 533 755.5 0 533 262 343 755.5 0 343 263 258 755.5 0 258 264 356 755.5 0 356 265 265 755.5 0 265 266 588 755.5 0 588 267 908 755.5 0 908 268 1279 755.5 0 1279 269 3948 755.5 0 3948 270 1567 755.5 0 1567 271 2436 755.5 0 2436 272 595 755.5 0 595 273 1061 755.5 0 1061 274 799 755.5 0 799 275 1552 755.5 0 1552 276 588 755.5 0 588 277 296 755.5 0 296 278 244 755.5 0 244 279 404 755.5 0 404 280 724 755.5 0 724 281 1629 755.5 0 1629 282 1297 755.5 0 1297 283 776 755.5 0 776 284 505 755.5 0 505 285 522 755.5 0 522 286 460 755.5 0 460 287 903 755.5 0 903 288 864 755.5 0 864 289 1153 755.5 0 1153 290 637 755.5 0 637 291 552 755.5 0 552 292 721 755.5 0 721 293 916 755.5 0 916 294 794 755.5 0 794 295 1142 755.5 0 1142 296 1119 755.5 0 1119 297 747 755.5 0 747 298 677 755.5 0 677 299 1999 755.5 0 1999 300 1166 755.5 0 1166 301 2565 755.5 0 2565 Finished in 2.51 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 63,863 Reads with adapters: 1,165 (1.8%) Reads that were too short: 13 (0.0%) Reads written (passing filters): 1,152 (1.8%) Total basepairs processed: 10,373,407 bp Total written (filtered): 310,068 bp (3.0%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 1165 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 13 0.0 3 0 12 0 1 20 1097 0.0 4 1059 31 4 0 3 21 5 0.0 4 0 5 22 1 0.0 4 1 23 1 0.0 4 0 0 0 1 26 28 0.0 4 0 0 0 0 28 35 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 51 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 90 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 0 1 104 1 0.0 4 0 0 0 0 1 106 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 145 1 0.0 4 0 0 0 1 149 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 193 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.05 s (43 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 1,152 Reads with adapters: 158 (13.7%) Reads written (passing filters): 1,152 (100.0%) Total basepairs processed: 310,068 bp Total written (filtered): 307,633 bp (99.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 158 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.2% C: 3.8% G: 92.4% T: 0.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 10 3 0.0 1 0 3 11 152 0.0 1 0 152 209 1 0.0 1 1 260 1 0.0 1 1 264 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.