This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCTCCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 90.45 s (29 us/read; 2.05 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 97,622 (3.2%) Reads that were too short: 2,760 (0.1%) Reads written (passing filters): 94,862 (3.1%) Total basepairs processed: 931,490,553 bp Total written (filtered): 16,881,578 bp (1.8%) === Adapter 1 === Sequence: GCTCCG; Type: regular 5'; Length: 6; Trimmed: 97622 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2525 755.5 0 2525 7 10062 755.5 0 10062 8 157 755.5 0 157 9 3648 755.5 0 3648 10 19 755.5 0 19 11 17 755.5 0 17 12 13 755.5 0 13 13 21 755.5 0 21 14 9 755.5 0 9 15 16 755.5 0 16 16 12 755.5 0 12 17 22 755.5 0 22 18 27 755.5 0 27 19 53 755.5 0 53 20 124 755.5 0 124 21 36 755.5 0 36 22 15 755.5 0 15 23 12 755.5 0 12 24 15 755.5 0 15 25 14 755.5 0 14 26 16 755.5 0 16 27 35 755.5 0 35 28 39 755.5 0 39 29 18 755.5 0 18 30 47 755.5 0 47 31 13 755.5 0 13 32 16 755.5 0 16 33 9 755.5 0 9 34 6 755.5 0 6 35 21 755.5 0 21 36 19 755.5 0 19 37 10 755.5 0 10 38 13 755.5 0 13 39 11 755.5 0 11 40 22 755.5 0 22 41 14 755.5 0 14 42 10 755.5 0 10 43 9 755.5 0 9 44 18 755.5 0 18 45 15 755.5 0 15 46 9 755.5 0 9 47 9 755.5 0 9 48 11 755.5 0 11 49 7 755.5 0 7 50 8 755.5 0 8 51 15 755.5 0 15 52 10 755.5 0 10 53 5 755.5 0 5 54 8 755.5 0 8 55 41 755.5 0 41 56 10 755.5 0 10 57 20 755.5 0 20 58 25 755.5 0 25 59 15 755.5 0 15 60 14 755.5 0 14 61 13 755.5 0 13 62 13 755.5 0 13 63 17 755.5 0 17 64 3 755.5 0 3 65 10 755.5 0 10 66 7 755.5 0 7 67 14 755.5 0 14 68 10 755.5 0 10 69 16 755.5 0 16 70 9 755.5 0 9 71 12 755.5 0 12 72 8 755.5 0 8 73 16 755.5 0 16 74 9 755.5 0 9 75 16 755.5 0 16 76 11 755.5 0 11 77 15 755.5 0 15 78 12 755.5 0 12 79 11 755.5 0 11 80 7 755.5 0 7 81 13 755.5 0 13 82 18 755.5 0 18 83 15 755.5 0 15 84 10 755.5 0 10 85 19 755.5 0 19 86 14 755.5 0 14 87 23 755.5 0 23 88 14 755.5 0 14 89 8 755.5 0 8 90 11 755.5 0 11 91 6 755.5 0 6 92 14 755.5 0 14 93 11 755.5 0 11 94 13 755.5 0 13 95 15 755.5 0 15 96 16 755.5 0 16 97 15 755.5 0 15 98 10 755.5 0 10 99 5 755.5 0 5 100 14 755.5 0 14 101 19 755.5 0 19 102 13 755.5 0 13 103 11 755.5 0 11 104 16 755.5 0 16 105 26 755.5 0 26 106 17 755.5 0 17 107 38 755.5 0 38 108 12 755.5 0 12 109 27 755.5 0 27 110 13 755.5 0 13 111 10 755.5 0 10 112 16 755.5 0 16 113 16 755.5 0 16 114 19 755.5 0 19 115 41 755.5 0 41 116 545 755.5 0 545 117 18 755.5 0 18 118 19 755.5 0 19 119 17 755.5 0 17 120 14 755.5 0 14 121 25 755.5 0 25 122 23 755.5 0 23 123 33 755.5 0 33 124 21 755.5 0 21 125 19 755.5 0 19 126 53 755.5 0 53 127 31 755.5 0 31 128 69 755.5 0 69 129 88 755.5 0 88 130 55 755.5 0 55 131 58 755.5 0 58 132 100 755.5 0 100 133 171 755.5 0 171 134 446 755.5 0 446 135 104 755.5 0 104 136 132 755.5 0 132 137 345 755.5 0 345 138 188 755.5 0 188 139 168 755.5 0 168 140 420 755.5 0 420 141 290 755.5 0 290 142 248 755.5 0 248 143 395 755.5 0 395 144 480 755.5 0 480 145 654 755.5 0 654 146 774 755.5 0 774 147 14861 755.5 0 14861 148 11467 755.5 0 11467 149 40758 755.5 0 40758 150 318 755.5 0 318 151 13 755.5 0 13 152 15 755.5 0 15 153 15 755.5 0 15 154 40 755.5 0 40 155 32 755.5 0 32 156 48 755.5 0 48 157 8 755.5 0 8 158 10 755.5 0 10 159 14 755.5 0 14 160 19 755.5 0 19 161 9 755.5 0 9 162 13 755.5 0 13 163 8 755.5 0 8 164 12 755.5 0 12 165 18 755.5 0 18 166 9 755.5 0 9 167 9 755.5 0 9 168 25 755.5 0 25 169 20 755.5 0 20 170 38 755.5 0 38 171 15 755.5 0 15 172 25 755.5 0 25 173 165 755.5 0 165 174 19 755.5 0 19 175 17 755.5 0 17 176 4 755.5 0 4 177 10 755.5 0 10 178 12 755.5 0 12 179 11 755.5 0 11 180 14 755.5 0 14 181 5 755.5 0 5 182 15 755.5 0 15 183 15 755.5 0 15 184 29 755.5 0 29 185 18 755.5 0 18 186 27 755.5 0 27 187 48 755.5 0 48 188 696 755.5 0 696 189 45 755.5 0 45 190 39 755.5 0 39 191 7 755.5 0 7 192 62 755.5 0 62 193 8 755.5 0 8 194 18 755.5 0 18 195 17 755.5 0 17 196 15 755.5 0 15 197 35 755.5 0 35 198 32 755.5 0 32 199 63 755.5 0 63 200 232 755.5 0 232 201 1190 755.5 0 1190 202 765 755.5 0 765 203 489 755.5 0 489 204 78 755.5 0 78 205 10 755.5 0 10 206 13 755.5 0 13 207 8 755.5 0 8 208 16 755.5 0 16 209 9 755.5 0 9 210 14 755.5 0 14 211 10 755.5 0 10 212 14 755.5 0 14 213 13 755.5 0 13 214 10 755.5 0 10 215 7 755.5 0 7 216 10 755.5 0 10 217 7 755.5 0 7 218 7 755.5 0 7 219 5 755.5 0 5 220 2 755.5 0 2 221 7 755.5 0 7 222 8 755.5 0 8 223 14 755.5 0 14 224 8 755.5 0 8 225 14 755.5 0 14 226 18 755.5 0 18 227 8 755.5 0 8 228 27 755.5 0 27 229 11 755.5 0 11 230 23 755.5 0 23 231 6 755.5 0 6 232 10 755.5 0 10 233 7 755.5 0 7 234 6 755.5 0 6 235 10 755.5 0 10 236 9 755.5 0 9 237 9 755.5 0 9 238 18 755.5 0 18 239 8 755.5 0 8 240 5 755.5 0 5 241 18 755.5 0 18 242 38 755.5 0 38 243 14 755.5 0 14 244 9 755.5 0 9 245 11 755.5 0 11 246 5 755.5 0 5 247 7 755.5 0 7 248 5 755.5 0 5 249 6 755.5 0 6 250 16 755.5 0 16 251 7 755.5 0 7 252 14 755.5 0 14 253 19 755.5 0 19 254 28 755.5 0 28 255 9 755.5 0 9 256 8 755.5 0 8 257 4 755.5 0 4 258 18 755.5 0 18 259 50 755.5 0 50 260 13 755.5 0 13 261 6 755.5 0 6 262 4 755.5 0 4 263 3 755.5 0 3 264 6 755.5 0 6 265 15 755.5 0 15 266 7 755.5 0 7 267 12 755.5 0 12 268 6 755.5 0 6 269 32 755.5 0 32 270 22 755.5 0 22 271 3 755.5 0 3 272 10 755.5 0 10 273 35 755.5 0 35 274 17 755.5 0 17 275 35 755.5 0 35 276 21 755.5 0 21 277 59 755.5 0 59 278 10 755.5 0 10 279 10 755.5 0 10 280 11 755.5 0 11 281 9 755.5 0 9 282 12 755.5 0 12 283 9 755.5 0 9 284 14 755.5 0 14 285 206 755.5 0 206 286 12 755.5 0 12 287 9 755.5 0 9 288 4 755.5 0 4 289 6 755.5 0 6 290 11 755.5 0 11 291 17 755.5 0 17 292 12 755.5 0 12 293 10 755.5 0 10 294 32 755.5 0 32 295 4 755.5 0 4 296 6 755.5 0 6 297 7 755.5 0 7 298 6 755.5 0 6 299 7 755.5 0 7 300 19 755.5 0 19 301 12 755.5 0 12 Finished in 3.54 s (37 us/read; 1.61 M reads/minute). === Summary === Total reads processed: 94,862 Reads with adapters: 12,517 (13.2%) Reads that were too short: 18 (0.0%) Reads written (passing filters): 12,499 (13.2%) Total basepairs processed: 16,881,578 bp Total written (filtered): 3,450,830 bp (20.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 12517 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 3 0.0 3 0 0 3 16 66 0.0 3 0 65 0 1 17 10120 0.0 3 9968 147 5 18 61 0.0 3 5 50 6 19 2174 0.0 3 2129 32 5 8 20 12 0.0 3 0 6 0 6 21 8 0.0 3 0 0 7 1 22 2 0.0 3 0 0 0 2 37 1 0.0 3 0 0 0 1 45 39 0.0 3 0 0 0 39 46 7 0.0 3 0 0 0 7 66 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 79 10 0.0 3 0 0 0 10 87 1 0.0 3 0 0 0 1 172 7 0.0 3 0 0 0 7 192 1 0.0 3 0 0 0 1 217 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.55 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 12,499 Reads with adapters: 1,331 (10.6%) Reads written (passing filters): 12,499 (100.0%) Total basepairs processed: 3,450,830 bp Total written (filtered): 3,446,779 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1331 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 18.0% C: 23.4% G: 55.0% T: 3.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1277 195.3 0 1277 4 52 48.8 0 52 5 1 12.2 0 1 7 1 0.8 0 1