This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACACAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 91.28 s (29 us/read; 2.03 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 599,108 (19.4%) Reads that were too short: 228,820 (7.4%) Reads written (passing filters): 370,288 (12.0%) Total basepairs processed: 931,490,553 bp Total written (filtered): 62,737,399 bp (6.7%) === Adapter 1 === Sequence: ACACAA; Type: regular 5'; Length: 6; Trimmed: 599108 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3530 755.5 0 3530 7 3455 755.5 0 3455 8 146 755.5 0 146 9 6070 755.5 0 6070 10 14 755.5 0 14 11 6 755.5 0 6 12 3 755.5 0 3 13 4 755.5 0 4 14 6 755.5 0 6 15 10 755.5 0 10 16 4 755.5 0 4 17 10 755.5 0 10 18 4 755.5 0 4 19 6 755.5 0 6 20 4 755.5 0 4 21 5 755.5 0 5 22 10 755.5 0 10 23 3 755.5 0 3 24 3 755.5 0 3 25 6 755.5 0 6 26 16 755.5 0 16 27 8 755.5 0 8 28 15 755.5 0 15 29 11 755.5 0 11 30 22 755.5 0 22 31 18 755.5 0 18 32 44 755.5 0 44 33 26 755.5 0 26 34 17 755.5 0 17 35 7 755.5 0 7 36 129 755.5 0 129 37 9 755.5 0 9 38 43 755.5 0 43 39 10 755.5 0 10 40 17 755.5 0 17 41 64 755.5 0 64 42 50 755.5 0 50 43 176 755.5 0 176 44 10 755.5 0 10 45 3 755.5 0 3 46 8 755.5 0 8 47 6 755.5 0 6 48 9 755.5 0 9 49 11 755.5 0 11 50 6 755.5 0 6 51 10 755.5 0 10 52 16 755.5 0 16 53 8 755.5 0 8 54 3 755.5 0 3 55 11 755.5 0 11 56 9 755.5 0 9 57 15 755.5 0 15 58 15 755.5 0 15 59 38 755.5 0 38 60 12 755.5 0 12 61 9 755.5 0 9 62 6 755.5 0 6 63 9 755.5 0 9 64 12 755.5 0 12 65 3 755.5 0 3 66 6 755.5 0 6 67 12 755.5 0 12 68 43 755.5 0 43 69 313 755.5 0 313 70 29 755.5 0 29 71 51 755.5 0 51 72 38 755.5 0 38 73 53 755.5 0 53 74 52 755.5 0 52 75 108 755.5 0 108 76 101 755.5 0 101 77 557 755.5 0 557 78 1364 755.5 0 1364 79 2097 755.5 0 2097 80 2638 755.5 0 2638 81 3703 755.5 0 3703 82 3776 755.5 0 3776 83 3599 755.5 0 3599 84 3673 755.5 0 3673 85 4685 755.5 0 4685 86 3658 755.5 0 3658 87 1726 755.5 0 1726 88 1617 755.5 0 1617 89 3471 755.5 0 3471 90 1492 755.5 0 1492 91 7572 755.5 0 7572 92 891 755.5 0 891 93 3394 755.5 0 3394 94 2221 755.5 0 2221 95 6781 755.5 0 6781 96 3116 755.5 0 3116 97 1473 755.5 0 1473 98 542 755.5 0 542 99 1160 755.5 0 1160 100 936 755.5 0 936 101 2400 755.5 0 2400 102 2293 755.5 0 2293 103 2891 755.5 0 2891 104 1902 755.5 0 1902 105 6423 755.5 0 6423 106 4549 755.5 0 4549 107 4166 755.5 0 4166 108 2081 755.5 0 2081 109 2006 755.5 0 2006 110 1850 755.5 0 1850 111 4518 755.5 0 4518 112 3366 755.5 0 3366 113 4819 755.5 0 4819 114 3292 755.5 0 3292 115 4173 755.5 0 4173 116 1402 755.5 0 1402 117 2253 755.5 0 2253 118 2363 755.5 0 2363 119 2936 755.5 0 2936 120 3218 755.5 0 3218 121 3579 755.5 0 3579 122 2544 755.5 0 2544 123 3231 755.5 0 3231 124 3271 755.5 0 3271 125 4125 755.5 0 4125 126 5061 755.5 0 5061 127 3819 755.5 0 3819 128 3233 755.5 0 3233 129 1689 755.5 0 1689 130 2776 755.5 0 2776 131 1700 755.5 0 1700 132 3532 755.5 0 3532 133 5652 755.5 0 5652 134 3846 755.5 0 3846 135 3426 755.5 0 3426 136 2291 755.5 0 2291 137 2159 755.5 0 2159 138 1929 755.5 0 1929 139 2222 755.5 0 2222 140 2741 755.5 0 2741 141 3423 755.5 0 3423 142 2113 755.5 0 2113 143 2332 755.5 0 2332 144 2138 755.5 0 2138 145 3371 755.5 0 3371 146 4142 755.5 0 4142 147 2766 755.5 0 2766 148 4039 755.5 0 4039 149 3320 755.5 0 3320 150 4024 755.5 0 4024 151 2482 755.5 0 2482 152 3278 755.5 0 3278 153 4005 755.5 0 4005 154 3790 755.5 0 3790 155 2812 755.5 0 2812 156 2665 755.5 0 2665 157 2011 755.5 0 2011 158 2592 755.5 0 2592 159 2366 755.5 0 2366 160 2835 755.5 0 2835 161 3256 755.5 0 3256 162 4143 755.5 0 4143 163 4342 755.5 0 4342 164 3701 755.5 0 3701 165 3112 755.5 0 3112 166 2253 755.5 0 2253 167 1839 755.5 0 1839 168 1824 755.5 0 1824 169 2419 755.5 0 2419 170 2404 755.5 0 2404 171 1889 755.5 0 1889 172 3257 755.5 0 3257 173 2311 755.5 0 2311 174 4917 755.5 0 4917 175 2113 755.5 0 2113 176 1755 755.5 0 1755 177 1263 755.5 0 1263 178 2176 755.5 0 2176 179 2331 755.5 0 2331 180 2897 755.5 0 2897 181 2549 755.5 0 2549 182 1904 755.5 0 1904 183 2123 755.5 0 2123 184 1957 755.5 0 1957 185 2455 755.5 0 2455 186 3025 755.5 0 3025 187 2604 755.5 0 2604 188 2351 755.5 0 2351 189 1871 755.5 0 1871 190 2714 755.5 0 2714 191 3144 755.5 0 3144 192 3272 755.5 0 3272 193 2742 755.5 0 2742 194 2023 755.5 0 2023 195 1015 755.5 0 1015 196 1322 755.5 0 1322 197 1375 755.5 0 1375 198 2572 755.5 0 2572 199 4140 755.5 0 4140 200 1992 755.5 0 1992 201 1547 755.5 0 1547 202 1491 755.5 0 1491 203 1588 755.5 0 1588 204 1689 755.5 0 1689 205 2056 755.5 0 2056 206 2019 755.5 0 2019 207 1773 755.5 0 1773 208 1672 755.5 0 1672 209 1657 755.5 0 1657 210 1678 755.5 0 1678 211 2239 755.5 0 2239 212 3051 755.5 0 3051 213 19454 755.5 0 19454 214 7654 755.5 0 7654 215 6920 755.5 0 6920 216 1888 755.5 0 1888 217 1886 755.5 0 1886 218 2434 755.5 0 2434 219 2720 755.5 0 2720 220 2012 755.5 0 2012 221 1422 755.5 0 1422 222 1735 755.5 0 1735 223 1458 755.5 0 1458 224 2293 755.5 0 2293 225 2435 755.5 0 2435 226 2283 755.5 0 2283 227 2901 755.5 0 2901 228 2001 755.5 0 2001 229 2379 755.5 0 2379 230 2596 755.5 0 2596 231 2263 755.5 0 2263 232 1303 755.5 0 1303 233 1364 755.5 0 1364 234 1900 755.5 0 1900 235 1955 755.5 0 1955 236 2319 755.5 0 2319 237 2283 755.5 0 2283 238 2325 755.5 0 2325 239 2700 755.5 0 2700 240 2110 755.5 0 2110 241 1482 755.5 0 1482 242 1486 755.5 0 1486 243 1405 755.5 0 1405 244 1397 755.5 0 1397 245 1955 755.5 0 1955 246 2417 755.5 0 2417 247 3529 755.5 0 3529 248 2931 755.5 0 2931 249 2540 755.5 0 2540 250 2730 755.5 0 2730 251 2432 755.5 0 2432 252 1820 755.5 0 1820 253 2027 755.5 0 2027 254 1701 755.5 0 1701 255 2295 755.5 0 2295 256 2434 755.5 0 2434 257 3068 755.5 0 3068 258 2103 755.5 0 2103 259 2139 755.5 0 2139 260 1268 755.5 0 1268 261 1249 755.5 0 1249 262 1320 755.5 0 1320 263 1237 755.5 0 1237 264 1545 755.5 0 1545 265 1672 755.5 0 1672 266 1996 755.5 0 1996 267 2731 755.5 0 2731 268 2240 755.5 0 2240 269 2365 755.5 0 2365 270 1701 755.5 0 1701 271 1522 755.5 0 1522 272 1676 755.5 0 1676 273 2008 755.5 0 2008 274 2136 755.5 0 2136 275 1887 755.5 0 1887 276 1651 755.5 0 1651 277 1369 755.5 0 1369 278 1546 755.5 0 1546 279 2352 755.5 0 2352 280 2334 755.5 0 2334 281 1420 755.5 0 1420 282 1850 755.5 0 1850 283 1193 755.5 0 1193 284 2173 755.5 0 2173 285 1928 755.5 0 1928 286 2432 755.5 0 2432 287 2564 755.5 0 2564 288 1831 755.5 0 1831 289 1830 755.5 0 1830 290 1377 755.5 0 1377 291 4031 755.5 0 4031 292 1808 755.5 0 1808 293 2372 755.5 0 2372 294 2070 755.5 0 2070 295 2127 755.5 0 2127 296 1374 755.5 0 1374 297 1127 755.5 0 1127 298 706 755.5 0 706 299 768 755.5 0 768 300 1184 755.5 0 1184 301 5053 755.5 0 5053 Finished in 12.42 s (34 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 370,288 Reads with adapters: 6,334 (1.7%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 6,315 (1.7%) Total basepairs processed: 62,737,399 bp Total written (filtered): 1,731,704 bp (2.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 6334 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 2 1 16 35 0.0 3 0 35 17 6195 0.0 3 6070 122 2 1 18 24 0.0 3 1 18 5 19 9 0.0 3 0 0 5 4 20 16 0.0 3 0 0 1 15 21 3 0.0 3 0 0 0 3 22 1 0.0 3 0 0 0 1 23 1 0.0 3 0 1 25 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 0 2 41 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 50 1 0.0 3 0 0 0 1 55 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 69 1 0.0 3 0 0 0 1 70 2 0.0 3 0 0 0 2 87 2 0.0 3 0 0 0 2 90 2 0.0 3 0 0 0 2 94 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 133 1 0.0 3 0 0 0 1 137 1 0.0 3 0 0 0 1 142 1 0.0 3 0 0 0 1 149 1 0.0 3 0 0 0 1 152 1 0.0 3 0 0 0 1 154 2 0.0 3 0 0 0 2 156 2 0.0 3 0 0 0 2 161 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 166 5 0.0 3 0 0 0 5 175 1 0.0 3 0 0 0 1 185 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.27 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 6,315 Reads with adapters: 562 (8.9%) Reads written (passing filters): 6,315 (100.0%) Total basepairs processed: 1,731,704 bp Total written (filtered): 1,729,997 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 562 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 63.9% C: 11.0% G: 21.9% T: 3.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 550 98.7 0 550 4 8 24.7 0 8 5 2 6.2 0 2 7 1 0.4 0 1 8 1 0.1 0 1