This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 94.32 s (30 us/read; 1.97 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 156,723 (5.1%) Reads that were too short: 74,247 (2.4%) Reads written (passing filters): 82,476 (2.7%) Total basepairs processed: 931,490,553 bp Total written (filtered): 14,406,273 bp (1.5%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 156723 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 180 755.5 0 180 7 63 755.5 0 63 8 163 755.5 0 163 9 5806 755.5 0 5806 10 17 755.5 0 17 11 6 755.5 0 6 12 8 755.5 0 8 13 4 755.5 0 4 14 4 755.5 0 4 15 10 755.5 0 10 16 5 755.5 0 5 17 3 755.5 0 3 18 1 755.5 0 1 19 8 755.5 0 8 20 3 755.5 0 3 21 3 755.5 0 3 22 4 755.5 0 4 23 4 755.5 0 4 24 15 755.5 0 15 25 12 755.5 0 12 26 8 755.5 0 8 27 5 755.5 0 5 28 6 755.5 0 6 29 5 755.5 0 5 30 6 755.5 0 6 31 10 755.5 0 10 32 8 755.5 0 8 33 5 755.5 0 5 34 8 755.5 0 8 35 12 755.5 0 12 36 7 755.5 0 7 37 10 755.5 0 10 38 9 755.5 0 9 39 12 755.5 0 12 40 9 755.5 0 9 41 9 755.5 0 9 42 10 755.5 0 10 43 6 755.5 0 6 44 3 755.5 0 3 45 4 755.5 0 4 46 4 755.5 0 4 47 4 755.5 0 4 48 9 755.5 0 9 49 10 755.5 0 10 50 5 755.5 0 5 51 21 755.5 0 21 52 18 755.5 0 18 53 118 755.5 0 118 54 2819 755.5 0 2819 55 54 755.5 0 54 56 15 755.5 0 15 57 13 755.5 0 13 58 9 755.5 0 9 59 10 755.5 0 10 60 19 755.5 0 19 61 11 755.5 0 11 62 25 755.5 0 25 63 55 755.5 0 55 64 23 755.5 0 23 65 67 755.5 0 67 66 37 755.5 0 37 67 94 755.5 0 94 68 221 755.5 0 221 69 3379 755.5 0 3379 70 904 755.5 0 904 71 1176 755.5 0 1176 72 14 755.5 0 14 73 16 755.5 0 16 74 26 755.5 0 26 75 66 755.5 0 66 76 60 755.5 0 60 77 15 755.5 0 15 78 117 755.5 0 117 79 105 755.5 0 105 80 320 755.5 0 320 81 335 755.5 0 335 82 324 755.5 0 324 83 95 755.5 0 95 84 316 755.5 0 316 85 538 755.5 0 538 86 258 755.5 0 258 87 278 755.5 0 278 88 2563 755.5 0 2563 89 152 755.5 0 152 90 102 755.5 0 102 91 373 755.5 0 373 92 204 755.5 0 204 93 97 755.5 0 97 94 350 755.5 0 350 95 281 755.5 0 281 96 391 755.5 0 391 97 580 755.5 0 580 98 569 755.5 0 569 99 287 755.5 0 287 100 289 755.5 0 289 101 839 755.5 0 839 102 3522 755.5 0 3522 103 285 755.5 0 285 104 350 755.5 0 350 105 187 755.5 0 187 106 77 755.5 0 77 107 114 755.5 0 114 108 403 755.5 0 403 109 181 755.5 0 181 110 42 755.5 0 42 111 132 755.5 0 132 112 248 755.5 0 248 113 231 755.5 0 231 114 497 755.5 0 497 115 184 755.5 0 184 116 243 755.5 0 243 117 370 755.5 0 370 118 414 755.5 0 414 119 280 755.5 0 280 120 612 755.5 0 612 121 357 755.5 0 357 122 440 755.5 0 440 123 390 755.5 0 390 124 361 755.5 0 361 125 327 755.5 0 327 126 389 755.5 0 389 127 325 755.5 0 325 128 343 755.5 0 343 129 475 755.5 0 475 130 344 755.5 0 344 131 527 755.5 0 527 132 703 755.5 0 703 133 1212 755.5 0 1212 134 1077 755.5 0 1077 135 356 755.5 0 356 136 526 755.5 0 526 137 686 755.5 0 686 138 1145 755.5 0 1145 139 577 755.5 0 577 140 598 755.5 0 598 141 303 755.5 0 303 142 418 755.5 0 418 143 569 755.5 0 569 144 315 755.5 0 315 145 269 755.5 0 269 146 290 755.5 0 290 147 608 755.5 0 608 148 821 755.5 0 821 149 529 755.5 0 529 150 628 755.5 0 628 151 459 755.5 0 459 152 447 755.5 0 447 153 301 755.5 0 301 154 297 755.5 0 297 155 407 755.5 0 407 156 486 755.5 0 486 157 429 755.5 0 429 158 633 755.5 0 633 159 723 755.5 0 723 160 752 755.5 0 752 161 482 755.5 0 482 162 489 755.5 0 489 163 438 755.5 0 438 164 504 755.5 0 504 165 401 755.5 0 401 166 387 755.5 0 387 167 463 755.5 0 463 168 659 755.5 0 659 169 770 755.5 0 770 170 460 755.5 0 460 171 721 755.5 0 721 172 678 755.5 0 678 173 327 755.5 0 327 174 304 755.5 0 304 175 713 755.5 0 713 176 2143 755.5 0 2143 177 1702 755.5 0 1702 178 962 755.5 0 962 179 628 755.5 0 628 180 332 755.5 0 332 181 462 755.5 0 462 182 539 755.5 0 539 183 761 755.5 0 761 184 917 755.5 0 917 185 740 755.5 0 740 186 719 755.5 0 719 187 871 755.5 0 871 188 1179 755.5 0 1179 189 1575 755.5 0 1575 190 364 755.5 0 364 191 233 755.5 0 233 192 252 755.5 0 252 193 615 755.5 0 615 194 579 755.5 0 579 195 661 755.5 0 661 196 991 755.5 0 991 197 449 755.5 0 449 198 259 755.5 0 259 199 399 755.5 0 399 200 762 755.5 0 762 201 821 755.5 0 821 202 491 755.5 0 491 203 384 755.5 0 384 204 906 755.5 0 906 205 655 755.5 0 655 206 528 755.5 0 528 207 529 755.5 0 529 208 904 755.5 0 904 209 504 755.5 0 504 210 323 755.5 0 323 211 421 755.5 0 421 212 469 755.5 0 469 213 741 755.5 0 741 214 649 755.5 0 649 215 670 755.5 0 670 216 687 755.5 0 687 217 665 755.5 0 665 218 948 755.5 0 948 219 1010 755.5 0 1010 220 594 755.5 0 594 221 793 755.5 0 793 222 765 755.5 0 765 223 582 755.5 0 582 224 717 755.5 0 717 225 742 755.5 0 742 226 538 755.5 0 538 227 500 755.5 0 500 228 507 755.5 0 507 229 768 755.5 0 768 230 763 755.5 0 763 231 583 755.5 0 583 232 513 755.5 0 513 233 667 755.5 0 667 234 350 755.5 0 350 235 326 755.5 0 326 236 664 755.5 0 664 237 876 755.5 0 876 238 1220 755.5 0 1220 239 943 755.5 0 943 240 632 755.5 0 632 241 525 755.5 0 525 242 540 755.5 0 540 243 668 755.5 0 668 244 1838 755.5 0 1838 245 894 755.5 0 894 246 692 755.5 0 692 247 492 755.5 0 492 248 644 755.5 0 644 249 780 755.5 0 780 250 811 755.5 0 811 251 835 755.5 0 835 252 1274 755.5 0 1274 253 3385 755.5 0 3385 254 523 755.5 0 523 255 694 755.5 0 694 256 586 755.5 0 586 257 514 755.5 0 514 258 666 755.5 0 666 259 877 755.5 0 877 260 649 755.5 0 649 261 652 755.5 0 652 262 454 755.5 0 454 263 595 755.5 0 595 264 329 755.5 0 329 265 392 755.5 0 392 266 409 755.5 0 409 267 610 755.5 0 610 268 987 755.5 0 987 269 1210 755.5 0 1210 270 986 755.5 0 986 271 1074 755.5 0 1074 272 723 755.5 0 723 273 866 755.5 0 866 274 847 755.5 0 847 275 784 755.5 0 784 276 1091 755.5 0 1091 277 1004 755.5 0 1004 278 1135 755.5 0 1135 279 589 755.5 0 589 280 542 755.5 0 542 281 977 755.5 0 977 282 992 755.5 0 992 283 1176 755.5 0 1176 284 1030 755.5 0 1030 285 495 755.5 0 495 286 352 755.5 0 352 287 447 755.5 0 447 288 563 755.5 0 563 289 831 755.5 0 831 290 685 755.5 0 685 291 724 755.5 0 724 292 606 755.5 0 606 293 599 755.5 0 599 294 869 755.5 0 869 295 1013 755.5 0 1013 296 724 755.5 0 724 297 528 755.5 0 528 298 351 755.5 0 351 299 561 755.5 0 561 300 568 755.5 0 568 301 1463 755.5 0 1463 Finished in 3.46 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 82,476 Reads with adapters: 6,063 (7.4%) Reads that were too short: 30 (0.0%) Reads written (passing filters): 6,033 (7.3%) Total basepairs processed: 14,406,273 bp Total written (filtered): 1,638,977 bp (11.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 6063 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 1 0.0 3 0 0 1 19 112 0.0 3 0 104 5 3 20 5871 0.0 4 5724 129 11 5 2 21 13 0.0 4 2 10 0 1 22 7 0.0 4 3 0 3 0 1 23 10 0.0 4 3 0 0 4 3 24 3 0.0 4 0 0 0 0 3 28 1 0.0 4 0 0 0 0 1 30 1 0.0 4 0 0 0 0 1 33 1 0.0 4 0 0 0 0 1 34 2 0.0 4 0 0 0 0 2 36 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 43 1 0.0 4 0 0 0 0 1 45 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 0 1 52 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 60 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 1 74 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 84 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 0 1 94 1 0.0 4 0 0 0 0 1 96 1 0.0 4 0 0 0 1 97 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 1 103 1 0.0 4 0 0 0 0 1 104 2 0.0 4 0 0 0 0 2 105 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 120 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 143 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 164 1 0.0 4 0 0 0 0 1 172 1 0.0 4 0 0 0 0 1 173 1 0.0 4 0 0 0 0 1 206 1 0.0 4 0 0 0 0 1 218 1 0.0 4 0 0 0 0 1 219 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.23 s (38 us/read; 1.57 M reads/minute). === Summary === Total reads processed: 6,033 Reads with adapters: 1,237 (20.5%) Reads written (passing filters): 6,033 (100.0%) Total basepairs processed: 1,638,977 bp Total written (filtered): 1,392,083 bp (84.9%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1237 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 76.8% C: 0.9% G: 21.6% T: 0.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 94.3 0 1 9 52 0.0 0 9 43 10 83 0.0 1 1 82 11 153 0.0 1 0 153 13 1 0.0 1 0 1 71 1 0.0 1 1 72 21 0.0 1 17 4 73 3 0.0 1 1 2 75 1 0.0 1 1 180 1 0.0 1 1 181 1 0.0 1 1 206 2 0.0 1 2 208 6 0.0 1 5 1 209 1 0.0 1 1 210 1 0.0 1 1 223 1 0.0 1 1 228 6 0.0 1 6 230 1 0.0 1 1 257 324 0.0 1 304 20 258 10 0.0 1 10 259 8 0.0 1 8 263 1 0.0 1 1 265 1 0.0 1 1 266 1 0.0 1 1 267 5 0.0 1 5 268 519 0.0 1 501 18 269 25 0.0 1 23 2 270 4 0.0 1 3 1 271 3 0.0 1 3