This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 92.86 s (30 us/read; 2.00 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 523,480 (16.9%) Reads that were too short: 7,751 (0.3%) Reads written (passing filters): 515,729 (16.7%) Total basepairs processed: 931,490,553 bp Total written (filtered): 150,209,175 bp (16.1%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 523480 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 8786 755.5 0 8786 7 492596 755.5 0 492596 8 1038 755.5 0 1038 9 91 755.5 0 91 10 68 755.5 0 68 11 23 755.5 0 23 12 9 755.5 0 9 13 11 755.5 0 11 14 15 755.5 0 15 15 15 755.5 0 15 16 12 755.5 0 12 17 12 755.5 0 12 18 7 755.5 0 7 19 16 755.5 0 16 20 33 755.5 0 33 21 29 755.5 0 29 22 52 755.5 0 52 23 185 755.5 0 185 24 171 755.5 0 171 25 244 755.5 0 244 26 9 755.5 0 9 27 7 755.5 0 7 28 7 755.5 0 7 29 8 755.5 0 8 30 2 755.5 0 2 31 13 755.5 0 13 32 11 755.5 0 11 33 13 755.5 0 13 34 21 755.5 0 21 35 17 755.5 0 17 36 15 755.5 0 15 37 15 755.5 0 15 38 9 755.5 0 9 39 18 755.5 0 18 40 17 755.5 0 17 41 7 755.5 0 7 42 8 755.5 0 8 43 9 755.5 0 9 44 13 755.5 0 13 45 6 755.5 0 6 46 5 755.5 0 5 47 7 755.5 0 7 48 9 755.5 0 9 49 10 755.5 0 10 50 18 755.5 0 18 51 8 755.5 0 8 52 8 755.5 0 8 53 8 755.5 0 8 54 21 755.5 0 21 55 15 755.5 0 15 56 12 755.5 0 12 57 11 755.5 0 11 58 8 755.5 0 8 59 9 755.5 0 9 60 13 755.5 0 13 61 7 755.5 0 7 62 4 755.5 0 4 63 9 755.5 0 9 64 10 755.5 0 10 65 12 755.5 0 12 66 8 755.5 0 8 67 15 755.5 0 15 68 10 755.5 0 10 69 6 755.5 0 6 70 10 755.5 0 10 71 9 755.5 0 9 72 9 755.5 0 9 73 16 755.5 0 16 74 13 755.5 0 13 75 26 755.5 0 26 76 9 755.5 0 9 77 12 755.5 0 12 78 5 755.5 0 5 79 6 755.5 0 6 80 13 755.5 0 13 81 22 755.5 0 22 82 14 755.5 0 14 83 20 755.5 0 20 84 19 755.5 0 19 85 15 755.5 0 15 86 13 755.5 0 13 87 26 755.5 0 26 88 85 755.5 0 85 89 122 755.5 0 122 90 200 755.5 0 200 91 7 755.5 0 7 92 14 755.5 0 14 93 10 755.5 0 10 94 21 755.5 0 21 95 26 755.5 0 26 96 72 755.5 0 72 97 99 755.5 0 99 98 87 755.5 0 87 99 38 755.5 0 38 100 23 755.5 0 23 101 239 755.5 0 239 102 44 755.5 0 44 103 276 755.5 0 276 104 36 755.5 0 36 105 28 755.5 0 28 106 48 755.5 0 48 107 20 755.5 0 20 108 9 755.5 0 9 109 16 755.5 0 16 110 27 755.5 0 27 111 47 755.5 0 47 112 48 755.5 0 48 113 78 755.5 0 78 114 84 755.5 0 84 115 27 755.5 0 27 116 22 755.5 0 22 117 48 755.5 0 48 118 84 755.5 0 84 119 46 755.5 0 46 120 42 755.5 0 42 121 39 755.5 0 39 122 44 755.5 0 44 123 48 755.5 0 48 124 69 755.5 0 69 125 75 755.5 0 75 126 208 755.5 0 208 127 6573 755.5 0 6573 128 738 755.5 0 738 129 781 755.5 0 781 130 17 755.5 0 17 131 8 755.5 0 8 132 61 755.5 0 61 133 17 755.5 0 17 134 5 755.5 0 5 135 14 755.5 0 14 136 9 755.5 0 9 137 9 755.5 0 9 138 10 755.5 0 10 139 8 755.5 0 8 140 11 755.5 0 11 141 7 755.5 0 7 142 15 755.5 0 15 143 14 755.5 0 14 144 12 755.5 0 12 145 127 755.5 0 127 146 22 755.5 0 22 147 6 755.5 0 6 148 22 755.5 0 22 149 69 755.5 0 69 150 9 755.5 0 9 151 10 755.5 0 10 152 2 755.5 0 2 153 4 755.5 0 4 154 4 755.5 0 4 155 3 755.5 0 3 156 10 755.5 0 10 157 11 755.5 0 11 158 12 755.5 0 12 159 9 755.5 0 9 160 4 755.5 0 4 161 13 755.5 0 13 162 7 755.5 0 7 163 7 755.5 0 7 164 11 755.5 0 11 165 5 755.5 0 5 166 3 755.5 0 3 167 18 755.5 0 18 168 10 755.5 0 10 169 5 755.5 0 5 170 7 755.5 0 7 171 7 755.5 0 7 172 5 755.5 0 5 173 5 755.5 0 5 174 5 755.5 0 5 175 9 755.5 0 9 176 10 755.5 0 10 177 8 755.5 0 8 178 9 755.5 0 9 179 5 755.5 0 5 180 7 755.5 0 7 181 11 755.5 0 11 182 12 755.5 0 12 183 23 755.5 0 23 184 7 755.5 0 7 185 6 755.5 0 6 186 4 755.5 0 4 187 6 755.5 0 6 188 6 755.5 0 6 189 10 755.5 0 10 190 9 755.5 0 9 191 4 755.5 0 4 192 7 755.5 0 7 193 17 755.5 0 17 194 17 755.5 0 17 195 43 755.5 0 43 196 18 755.5 0 18 197 7 755.5 0 7 198 6 755.5 0 6 199 4 755.5 0 4 200 17 755.5 0 17 201 12 755.5 0 12 202 6 755.5 0 6 203 16 755.5 0 16 204 14 755.5 0 14 205 8 755.5 0 8 206 13 755.5 0 13 207 12 755.5 0 12 208 24 755.5 0 24 209 14 755.5 0 14 210 11 755.5 0 11 211 6 755.5 0 6 212 5 755.5 0 5 213 17 755.5 0 17 214 19 755.5 0 19 215 21 755.5 0 21 216 10 755.5 0 10 217 5 755.5 0 5 218 7 755.5 0 7 219 8 755.5 0 8 220 14 755.5 0 14 221 8 755.5 0 8 222 10 755.5 0 10 223 31 755.5 0 31 224 15 755.5 0 15 225 57 755.5 0 57 226 8 755.5 0 8 227 5 755.5 0 5 228 7 755.5 0 7 229 8 755.5 0 8 230 7 755.5 0 7 231 10 755.5 0 10 232 4 755.5 0 4 233 20 755.5 0 20 234 9 755.5 0 9 235 23 755.5 0 23 236 26 755.5 0 26 237 18 755.5 0 18 238 9 755.5 0 9 239 13 755.5 0 13 240 5 755.5 0 5 241 37 755.5 0 37 242 26 755.5 0 26 243 26 755.5 0 26 244 9 755.5 0 9 245 30 755.5 0 30 246 23 755.5 0 23 247 33 755.5 0 33 248 13 755.5 0 13 249 27 755.5 0 27 250 18 755.5 0 18 251 15 755.5 0 15 252 65 755.5 0 65 253 114 755.5 0 114 254 119 755.5 0 119 255 46 755.5 0 46 256 103 755.5 0 103 257 51 755.5 0 51 258 44 755.5 0 44 259 185 755.5 0 185 260 318 755.5 0 318 261 1063 755.5 0 1063 262 3593 755.5 0 3593 263 36 755.5 0 36 264 6 755.5 0 6 265 9 755.5 0 9 266 7 755.5 0 7 267 24 755.5 0 24 268 16 755.5 0 16 269 8 755.5 0 8 270 4 755.5 0 4 271 3 755.5 0 3 272 7 755.5 0 7 273 36 755.5 0 36 274 9 755.5 0 9 275 13 755.5 0 13 276 10 755.5 0 10 277 7 755.5 0 7 278 26 755.5 0 26 279 34 755.5 0 34 280 322 755.5 0 322 281 157 755.5 0 157 282 7 755.5 0 7 283 20 755.5 0 20 284 7 755.5 0 7 285 12 755.5 0 12 286 11 755.5 0 11 287 15 755.5 0 15 288 47 755.5 0 47 289 27 755.5 0 27 290 11 755.5 0 11 291 3 755.5 0 3 292 9 755.5 0 9 293 47 755.5 0 47 294 14 755.5 0 14 295 16 755.5 0 16 296 17 755.5 0 17 297 16 755.5 0 16 298 46 755.5 0 46 299 108 755.5 0 108 300 53 755.5 0 53 301 50 755.5 0 50 Finished in 23.00 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 515,729 Reads with adapters: 501,719 (97.3%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 501,715 (97.3%) Total basepairs processed: 150,209,175 bp Total written (filtered): 137,479,415 bp (91.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 501719 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 17 4 0.0 3 0 0 0 1 3 18 182 0.0 3 0 0 128 41 13 19 5072 0.0 3 0 4703 232 94 43 20 494605 0.0 4 481001 11767 1346 377 114 21 1275 0.0 4 166 1076 21 9 3 22 111 0.0 4 12 0 89 3 7 23 442 0.0 4 3 0 4 361 74 24 9 0.0 4 0 0 0 0 9 25 5 0.0 4 1 1 2 0 1 27 2 0.0 4 2 28 3 0.0 4 2 1 29 2 0.0 4 2 30 1 0.0 4 1 33 1 0.0 4 1 34 1 0.0 4 1 121 1 0.0 4 0 0 0 0 1 162 1 0.0 4 0 0 0 0 1 186 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 19.67 s (39 us/read; 1.53 M reads/minute). === Summary === Total reads processed: 501,715 Reads with adapters: 20,751 (4.1%) Reads written (passing filters): 501,715 (100.0%) Total basepairs processed: 137,479,415 bp Total written (filtered): 137,164,557 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 20751 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.6% C: 3.3% G: 90.4% T: 1.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 114 7839.3 0 114 4 28 1959.8 0 28 5 7 490.0 0 7 8 2 7.7 0 2 9 22 1.9 0 7 15 10 33 0.5 1 0 33 11 19131 0.1 1 72 19059 12 833 0.0 1 23 810 13 21 0.0 1 0 21 14 3 0.0 1 0 3 15 45 0.0 1 0 45 16 6 0.0 1 0 6 58 49 0.0 1 40 9 59 2 0.0 1 2 61 4 0.0 1 4 93 1 0.0 1 1 124 1 0.0 1 1 138 1 0.0 1 1 152 88 0.0 1 74 14 153 3 0.0 1 3 159 1 0.0 1 1 162 1 0.0 1 1 163 1 0.0 1 1 164 1 0.0 1 1 179 1 0.0 1 1 191 10 0.0 1 2 8 208 1 0.0 1 1 210 320 0.0 1 270 50 211 13 0.0 1 10 3 229 1 0.0 1 1 230 2 0.0 1 1 1 260 1 0.0 1 1 261 1 0.0 1 1 268 1 0.0 1 0 1 269 1 0.0 1 1 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.