This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 90.99 s (29 us/read; 2.04 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 58,784 (1.9%) Reads that were too short: 20,322 (0.7%) Reads written (passing filters): 38,462 (1.2%) Total basepairs processed: 931,490,553 bp Total written (filtered): 8,683,267 bp (0.9%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 58784 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 124 755.5 0 124 7 123 755.5 0 123 8 371 755.5 0 371 9 12001 755.5 0 12001 10 26 755.5 0 26 11 17 755.5 0 17 12 3 755.5 0 3 13 4 755.5 0 4 14 6 755.5 0 6 15 16 755.5 0 16 16 2 755.5 0 2 17 17 755.5 0 17 18 14 755.5 0 14 19 12 755.5 0 12 20 6 755.5 0 6 21 4 755.5 0 4 22 8 755.5 0 8 23 6 755.5 0 6 24 7 755.5 0 7 25 19 755.5 0 19 26 51 755.5 0 51 27 30 755.5 0 30 28 88 755.5 0 88 29 120 755.5 0 120 30 108 755.5 0 108 31 245 755.5 0 245 32 4058 755.5 0 4058 33 26 755.5 0 26 34 11 755.5 0 11 35 3 755.5 0 3 36 11 755.5 0 11 37 11 755.5 0 11 38 11 755.5 0 11 39 5 755.5 0 5 40 11 755.5 0 11 41 5 755.5 0 5 42 5 755.5 0 5 43 17 755.5 0 17 44 10 755.5 0 10 45 200 755.5 0 200 46 88 755.5 0 88 47 358 755.5 0 358 48 7 755.5 0 7 49 3 755.5 0 3 50 5 755.5 0 5 51 8 755.5 0 8 52 12 755.5 0 12 53 7 755.5 0 7 54 3 755.5 0 3 55 3 755.5 0 3 56 6 755.5 0 6 57 10 755.5 0 10 58 9 755.5 0 9 59 8 755.5 0 8 60 15 755.5 0 15 61 9 755.5 0 9 62 4 755.5 0 4 63 1 755.5 0 1 64 4 755.5 0 4 65 1 755.5 0 1 66 6 755.5 0 6 68 4 755.5 0 4 69 4 755.5 0 4 70 10 755.5 0 10 71 109 755.5 0 109 72 104 755.5 0 104 73 14 755.5 0 14 74 41 755.5 0 41 75 28 755.5 0 28 76 79 755.5 0 79 77 326 755.5 0 326 78 152 755.5 0 152 79 138 755.5 0 138 80 518 755.5 0 518 81 186 755.5 0 186 82 260 755.5 0 260 83 628 755.5 0 628 84 564 755.5 0 564 85 70 755.5 0 70 86 47 755.5 0 47 87 109 755.5 0 109 88 28 755.5 0 28 89 28 755.5 0 28 90 325 755.5 0 325 91 134 755.5 0 134 92 275 755.5 0 275 93 795 755.5 0 795 94 129 755.5 0 129 95 195 755.5 0 195 96 81 755.5 0 81 97 170 755.5 0 170 98 102 755.5 0 102 99 97 755.5 0 97 100 125 755.5 0 125 101 48 755.5 0 48 102 12 755.5 0 12 103 23 755.5 0 23 104 20 755.5 0 20 105 17 755.5 0 17 106 22 755.5 0 22 107 46 755.5 0 46 108 27 755.5 0 27 109 48 755.5 0 48 110 128 755.5 0 128 111 87 755.5 0 87 112 53 755.5 0 53 113 305 755.5 0 305 114 379 755.5 0 379 115 1725 755.5 0 1725 116 236 755.5 0 236 117 94 755.5 0 94 118 200 755.5 0 200 119 111 755.5 0 111 120 86 755.5 0 86 121 84 755.5 0 84 122 81 755.5 0 81 123 111 755.5 0 111 124 110 755.5 0 110 125 99 755.5 0 99 126 80 755.5 0 80 127 53 755.5 0 53 128 117 755.5 0 117 129 258 755.5 0 258 130 205 755.5 0 205 131 289 755.5 0 289 132 135 755.5 0 135 133 162 755.5 0 162 134 134 755.5 0 134 135 520 755.5 0 520 136 325 755.5 0 325 137 298 755.5 0 298 138 249 755.5 0 249 139 196 755.5 0 196 140 152 755.5 0 152 141 124 755.5 0 124 142 117 755.5 0 117 143 267 755.5 0 267 144 143 755.5 0 143 145 135 755.5 0 135 146 83 755.5 0 83 147 65 755.5 0 65 148 51 755.5 0 51 149 53 755.5 0 53 150 86 755.5 0 86 151 80 755.5 0 80 152 100 755.5 0 100 153 97 755.5 0 97 154 141 755.5 0 141 155 123 755.5 0 123 156 112 755.5 0 112 157 143 755.5 0 143 158 95 755.5 0 95 159 71 755.5 0 71 160 72 755.5 0 72 161 56 755.5 0 56 162 111 755.5 0 111 163 124 755.5 0 124 164 150 755.5 0 150 165 132 755.5 0 132 166 105 755.5 0 105 167 151 755.5 0 151 168 152 755.5 0 152 169 113 755.5 0 113 170 87 755.5 0 87 171 122 755.5 0 122 172 69 755.5 0 69 173 76 755.5 0 76 174 74 755.5 0 74 175 61 755.5 0 61 176 35 755.5 0 35 177 107 755.5 0 107 178 211 755.5 0 211 179 185 755.5 0 185 180 186 755.5 0 186 181 108 755.5 0 108 182 106 755.5 0 106 183 115 755.5 0 115 184 138 755.5 0 138 185 103 755.5 0 103 186 66 755.5 0 66 187 82 755.5 0 82 188 78 755.5 0 78 189 146 755.5 0 146 190 285 755.5 0 285 191 240 755.5 0 240 192 125 755.5 0 125 193 77 755.5 0 77 194 39 755.5 0 39 195 91 755.5 0 91 196 115 755.5 0 115 197 102 755.5 0 102 198 166 755.5 0 166 199 80 755.5 0 80 200 62 755.5 0 62 201 94 755.5 0 94 202 699 755.5 0 699 203 2220 755.5 0 2220 204 848 755.5 0 848 205 103 755.5 0 103 206 99 755.5 0 99 207 84 755.5 0 84 208 121 755.5 0 121 209 253 755.5 0 253 210 511 755.5 0 511 211 235 755.5 0 235 212 191 755.5 0 191 213 88 755.5 0 88 214 111 755.5 0 111 215 197 755.5 0 197 216 172 755.5 0 172 217 193 755.5 0 193 218 111 755.5 0 111 219 63 755.5 0 63 220 91 755.5 0 91 221 125 755.5 0 125 222 94 755.5 0 94 223 149 755.5 0 149 224 264 755.5 0 264 225 423 755.5 0 423 226 332 755.5 0 332 227 779 755.5 0 779 228 518 755.5 0 518 229 63 755.5 0 63 230 47 755.5 0 47 231 115 755.5 0 115 232 155 755.5 0 155 233 171 755.5 0 171 234 339 755.5 0 339 235 438 755.5 0 438 236 709 755.5 0 709 237 937 755.5 0 937 238 265 755.5 0 265 239 66 755.5 0 66 240 99 755.5 0 99 241 159 755.5 0 159 242 175 755.5 0 175 243 101 755.5 0 101 244 72 755.5 0 72 245 47 755.5 0 47 246 60 755.5 0 60 247 47 755.5 0 47 248 46 755.5 0 46 249 64 755.5 0 64 250 104 755.5 0 104 251 109 755.5 0 109 252 131 755.5 0 131 253 114 755.5 0 114 254 82 755.5 0 82 255 50 755.5 0 50 256 24 755.5 0 24 257 39 755.5 0 39 258 36 755.5 0 36 259 136 755.5 0 136 260 98 755.5 0 98 261 99 755.5 0 99 262 121 755.5 0 121 263 185 755.5 0 185 264 158 755.5 0 158 265 165 755.5 0 165 266 51 755.5 0 51 267 35 755.5 0 35 268 38 755.5 0 38 269 51 755.5 0 51 270 318 755.5 0 318 271 237 755.5 0 237 272 102 755.5 0 102 273 69 755.5 0 69 274 87 755.5 0 87 275 145 755.5 0 145 276 121 755.5 0 121 277 132 755.5 0 132 278 122 755.5 0 122 279 77 755.5 0 77 280 93 755.5 0 93 281 63 755.5 0 63 282 263 755.5 0 263 283 83 755.5 0 83 284 71 755.5 0 71 285 55 755.5 0 55 286 61 755.5 0 61 287 49 755.5 0 49 288 111 755.5 0 111 289 307 755.5 0 307 290 268 755.5 0 268 291 188 755.5 0 188 292 168 755.5 0 168 293 92 755.5 0 92 294 130 755.5 0 130 295 227 755.5 0 227 296 265 755.5 0 265 297 507 755.5 0 507 298 148 755.5 0 148 299 187 755.5 0 187 300 248 755.5 0 248 301 253 755.5 0 253 Finished in 1.79 s (47 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 38,462 Reads with adapters: 12,427 (32.3%) Reads that were too short: 11 (0.0%) Reads written (passing filters): 12,416 (32.3%) Total basepairs processed: 8,683,267 bp Total written (filtered): 3,375,955 bp (38.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 12427 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 4 0.0 3 0 0 3 1 19 102 0.0 3 0 99 1 0 2 20 12230 0.0 4 11918 266 34 10 2 21 41 0.0 4 16 23 2 22 2 0.0 4 0 0 2 23 13 0.0 4 2 0 0 9 2 24 1 0.0 4 0 0 0 0 1 25 5 0.0 4 0 0 0 0 5 26 1 0.0 4 1 28 3 0.0 4 0 0 0 0 3 33 1 0.0 4 1 37 1 0.0 4 0 0 0 0 1 42 1 0.0 4 0 0 0 0 1 47 3 0.0 4 0 0 0 0 3 49 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 89 2 0.0 4 0 0 0 0 2 90 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 102 1 0.0 4 0 0 0 0 1 127 2 0.0 4 0 0 0 0 2 129 1 0.0 4 0 0 0 0 1 132 1 0.0 4 0 0 0 0 1 141 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.48 s (39 us/read; 1.55 M reads/minute). === Summary === Total reads processed: 12,416 Reads with adapters: 1,294 (10.4%) Reads written (passing filters): 12,416 (100.0%) Total basepairs processed: 3,375,955 bp Total written (filtered): 3,360,778 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1294 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.2% C: 2.0% G: 94.0% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 194.0 0 1 6 1 3.0 0 1 9 85 0.0 0 11 74 10 162 0.0 1 8 154 11 1035 0.0 1 1 1034 12 1 0.0 1 0 1 13 3 0.0 1 0 3 146 1 0.0 1 1 150 1 0.0 1 1 258 1 0.0 1 1 263 2 0.0 1 2 267 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.