This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCAGAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 91.89 s (30 us/read; 2.02 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 77,449 (2.5%) Reads that were too short: 12,987 (0.4%) Reads written (passing filters): 64,462 (2.1%) Total basepairs processed: 931,490,553 bp Total written (filtered): 17,327,388 bp (1.9%) === Adapter 1 === Sequence: TCAGAG; Type: regular 5'; Length: 6; Trimmed: 77449 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 478 755.5 0 478 7 621 755.5 0 621 8 14253 755.5 0 14253 9 37139 755.5 0 37139 10 61 755.5 0 61 11 4 755.5 0 4 12 2 755.5 0 2 13 3 755.5 0 3 15 2 755.5 0 2 16 1 755.5 0 1 17 2 755.5 0 2 18 1 755.5 0 1 19 2 755.5 0 2 20 3 755.5 0 3 21 1 755.5 0 1 22 3 755.5 0 3 23 7 755.5 0 7 24 3 755.5 0 3 25 1 755.5 0 1 26 2 755.5 0 2 27 1 755.5 0 1 28 5 755.5 0 5 30 4 755.5 0 4 31 4 755.5 0 4 32 4 755.5 0 4 33 6 755.5 0 6 34 9 755.5 0 9 35 1 755.5 0 1 36 1 755.5 0 1 37 4 755.5 0 4 38 17 755.5 0 17 39 10 755.5 0 10 40 5 755.5 0 5 41 3 755.5 0 3 42 6 755.5 0 6 43 6 755.5 0 6 44 10 755.5 0 10 45 4 755.5 0 4 46 2 755.5 0 2 47 4 755.5 0 4 48 6 755.5 0 6 49 20 755.5 0 20 50 11 755.5 0 11 51 6 755.5 0 6 52 6 755.5 0 6 53 4 755.5 0 4 54 7 755.5 0 7 55 10 755.5 0 10 56 9 755.5 0 9 57 8 755.5 0 8 58 6 755.5 0 6 59 32 755.5 0 32 60 450 755.5 0 450 61 53 755.5 0 53 62 243 755.5 0 243 63 8 755.5 0 8 64 112 755.5 0 112 65 83 755.5 0 83 66 792 755.5 0 792 67 280 755.5 0 280 68 26 755.5 0 26 69 4 755.5 0 4 70 5 755.5 0 5 71 6 755.5 0 6 72 8 755.5 0 8 73 3 755.5 0 3 74 4 755.5 0 4 75 4 755.5 0 4 76 8 755.5 0 8 77 57 755.5 0 57 78 12 755.5 0 12 79 49 755.5 0 49 80 11 755.5 0 11 81 77 755.5 0 77 82 20 755.5 0 20 83 8 755.5 0 8 84 2 755.5 0 2 85 5 755.5 0 5 86 10 755.5 0 10 87 6 755.5 0 6 88 6 755.5 0 6 89 5 755.5 0 5 90 14 755.5 0 14 91 6 755.5 0 6 92 26 755.5 0 26 93 9 755.5 0 9 94 13 755.5 0 13 95 20 755.5 0 20 96 18 755.5 0 18 97 298 755.5 0 298 98 35 755.5 0 35 99 61 755.5 0 61 100 3 755.5 0 3 101 13 755.5 0 13 102 16 755.5 0 16 103 25 755.5 0 25 104 9 755.5 0 9 105 10 755.5 0 10 106 15 755.5 0 15 107 14 755.5 0 14 108 28 755.5 0 28 109 24 755.5 0 24 110 9 755.5 0 9 111 22 755.5 0 22 112 82 755.5 0 82 113 97 755.5 0 97 114 78 755.5 0 78 115 1162 755.5 0 1162 116 536 755.5 0 536 117 81 755.5 0 81 118 14 755.5 0 14 119 9 755.5 0 9 120 14 755.5 0 14 121 24 755.5 0 24 122 34 755.5 0 34 123 24 755.5 0 24 124 31 755.5 0 31 125 21 755.5 0 21 126 15 755.5 0 15 127 41 755.5 0 41 128 30 755.5 0 30 129 17 755.5 0 17 130 25 755.5 0 25 131 14 755.5 0 14 132 25 755.5 0 25 133 25 755.5 0 25 134 24 755.5 0 24 135 35 755.5 0 35 136 44 755.5 0 44 137 50 755.5 0 50 138 36 755.5 0 36 139 45 755.5 0 45 140 22 755.5 0 22 141 24 755.5 0 24 142 28 755.5 0 28 143 36 755.5 0 36 144 27 755.5 0 27 145 18 755.5 0 18 146 51 755.5 0 51 147 20 755.5 0 20 148 34 755.5 0 34 149 19 755.5 0 19 150 19 755.5 0 19 151 44 755.5 0 44 152 19 755.5 0 19 153 18 755.5 0 18 154 23 755.5 0 23 155 18 755.5 0 18 156 54 755.5 0 54 157 46 755.5 0 46 158 58 755.5 0 58 159 43 755.5 0 43 160 37 755.5 0 37 161 66 755.5 0 66 162 47 755.5 0 47 163 52 755.5 0 52 164 98 755.5 0 98 165 70 755.5 0 70 166 353 755.5 0 353 167 290 755.5 0 290 168 41 755.5 0 41 169 63 755.5 0 63 170 62 755.5 0 62 171 50 755.5 0 50 172 77 755.5 0 77 173 35 755.5 0 35 174 18 755.5 0 18 175 24 755.5 0 24 176 54 755.5 0 54 177 51 755.5 0 51 178 54 755.5 0 54 179 60 755.5 0 60 180 52 755.5 0 52 181 143 755.5 0 143 182 57 755.5 0 57 183 54 755.5 0 54 184 76 755.5 0 76 185 45 755.5 0 45 186 112 755.5 0 112 187 66 755.5 0 66 188 67 755.5 0 67 189 1502 755.5 0 1502 190 438 755.5 0 438 191 563 755.5 0 563 192 61 755.5 0 61 193 59 755.5 0 59 194 117 755.5 0 117 195 40 755.5 0 40 196 72 755.5 0 72 197 48 755.5 0 48 198 29 755.5 0 29 199 30 755.5 0 30 200 41 755.5 0 41 201 69 755.5 0 69 202 104 755.5 0 104 203 102 755.5 0 102 204 59 755.5 0 59 205 56 755.5 0 56 206 47 755.5 0 47 207 50 755.5 0 50 208 51 755.5 0 51 209 49 755.5 0 49 210 35 755.5 0 35 211 51 755.5 0 51 212 70 755.5 0 70 213 72 755.5 0 72 214 64 755.5 0 64 215 51 755.5 0 51 216 68 755.5 0 68 217 108 755.5 0 108 218 60 755.5 0 60 219 71 755.5 0 71 220 91 755.5 0 91 221 56 755.5 0 56 222 53 755.5 0 53 223 17 755.5 0 17 224 28 755.5 0 28 225 69 755.5 0 69 226 81 755.5 0 81 227 73 755.5 0 73 228 81 755.5 0 81 229 59 755.5 0 59 230 62 755.5 0 62 231 79 755.5 0 79 232 118 755.5 0 118 233 85 755.5 0 85 234 67 755.5 0 67 235 75 755.5 0 75 236 230 755.5 0 230 237 196 755.5 0 196 238 121 755.5 0 121 239 78 755.5 0 78 240 76 755.5 0 76 241 53 755.5 0 53 242 47 755.5 0 47 243 57 755.5 0 57 244 73 755.5 0 73 245 943 755.5 0 943 246 2224 755.5 0 2224 247 1992 755.5 0 1992 248 77 755.5 0 77 249 75 755.5 0 75 250 28 755.5 0 28 251 17 755.5 0 17 252 26 755.5 0 26 253 30 755.5 0 30 254 68 755.5 0 68 255 82 755.5 0 82 256 147 755.5 0 147 257 69 755.5 0 69 258 62 755.5 0 62 259 164 755.5 0 164 260 438 755.5 0 438 261 80 755.5 0 80 262 96 755.5 0 96 263 53 755.5 0 53 264 66 755.5 0 66 265 107 755.5 0 107 266 87 755.5 0 87 267 418 755.5 0 418 268 245 755.5 0 245 269 93 755.5 0 93 270 97 755.5 0 97 271 73 755.5 0 73 272 60 755.5 0 60 273 33 755.5 0 33 274 29 755.5 0 29 275 49 755.5 0 49 276 60 755.5 0 60 277 87 755.5 0 87 278 75 755.5 0 75 279 65 755.5 0 65 280 61 755.5 0 61 281 55 755.5 0 55 282 94 755.5 0 94 283 77 755.5 0 77 284 68 755.5 0 68 285 69 755.5 0 69 286 79 755.5 0 79 287 92 755.5 0 92 288 105 755.5 0 105 289 69 755.5 0 69 290 50 755.5 0 50 291 29 755.5 0 29 292 27 755.5 0 27 293 64 755.5 0 64 294 83 755.5 0 83 295 193 755.5 0 193 296 106 755.5 0 106 297 40 755.5 0 40 298 30 755.5 0 30 299 35 755.5 0 35 300 38 755.5 0 38 301 15 755.5 0 15 Finished in 3.08 s (48 us/read; 1.26 M reads/minute). === Summary === Total reads processed: 64,462 Reads with adapters: 13,472 (20.9%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 13,455 (20.9%) Total basepairs processed: 17,327,388 bp Total written (filtered): 3,711,228 bp (21.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 13472 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 2 2 16 122 0.0 3 0 119 1 2 17 13198 0.0 3 13026 168 4 18 44 0.0 3 9 25 7 3 19 36 0.0 3 4 0 29 3 20 32 0.0 3 0 0 0 32 25 1 0.0 3 1 27 1 0.0 3 0 0 0 1 37 6 0.0 3 0 0 0 6 38 1 0.0 3 0 0 1 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 84 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 164 12 0.0 3 0 0 0 12 174 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 192 1 0.0 3 0 0 0 1 193 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 287 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.54 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 13,455 Reads with adapters: 141 (1.0%) Reads written (passing filters): 13,455 (100.0%) Total basepairs processed: 3,711,228 bp Total written (filtered): 3,710,800 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 141 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 7.1% C: 17.7% G: 70.9% T: 4.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 138 210.2 0 138 4 1 52.6 0 1 5 2 13.1 0 2