This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 88.89 s (29 us/read; 2.09 M reads/minute). === Summary === Total reads processed: 3,094,653 Reads with adapters: 29,552 (1.0%) Reads that were too short: 5,390 (0.2%) Reads written (passing filters): 24,162 (0.8%) Total basepairs processed: 931,490,553 bp Total written (filtered): 4,880,081 bp (0.5%) === Adapter 1 === Sequence: CCTGTC; Type: regular 5'; Length: 6; Trimmed: 29552 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 595 755.5 0 595 7 7228 755.5 0 7228 8 21 755.5 0 21 9 23 755.5 0 23 10 7 755.5 0 7 11 16 755.5 0 16 12 8 755.5 0 8 13 5 755.5 0 5 14 6 755.5 0 6 15 10 755.5 0 10 16 13 755.5 0 13 17 9 755.5 0 9 18 3 755.5 0 3 19 13 755.5 0 13 20 11 755.5 0 11 21 14 755.5 0 14 22 8 755.5 0 8 23 7 755.5 0 7 24 8 755.5 0 8 25 8 755.5 0 8 26 8 755.5 0 8 27 9 755.5 0 9 28 92 755.5 0 92 29 11 755.5 0 11 30 12 755.5 0 12 31 14 755.5 0 14 32 36 755.5 0 36 33 637 755.5 0 637 34 104 755.5 0 104 35 125 755.5 0 125 36 9 755.5 0 9 37 10 755.5 0 10 38 12 755.5 0 12 39 5 755.5 0 5 40 5 755.5 0 5 41 12 755.5 0 12 42 10 755.5 0 10 43 12 755.5 0 12 44 17 755.5 0 17 45 23 755.5 0 23 46 9 755.5 0 9 47 15 755.5 0 15 48 10 755.5 0 10 49 33 755.5 0 33 50 8 755.5 0 8 51 18 755.5 0 18 52 5 755.5 0 5 53 27 755.5 0 27 54 9 755.5 0 9 55 11 755.5 0 11 56 17 755.5 0 17 57 94 755.5 0 94 58 39 755.5 0 39 59 120 755.5 0 120 60 14 755.5 0 14 61 4 755.5 0 4 62 12 755.5 0 12 63 2 755.5 0 2 64 10 755.5 0 10 65 12 755.5 0 12 66 13 755.5 0 13 67 11 755.5 0 11 68 4 755.5 0 4 69 12 755.5 0 12 70 8 755.5 0 8 71 15 755.5 0 15 72 58 755.5 0 58 73 56 755.5 0 56 74 95 755.5 0 95 75 12 755.5 0 12 76 1 755.5 0 1 77 14 755.5 0 14 78 9 755.5 0 9 79 17 755.5 0 17 80 12 755.5 0 12 81 21 755.5 0 21 82 10 755.5 0 10 83 20 755.5 0 20 84 108 755.5 0 108 85 23 755.5 0 23 86 28 755.5 0 28 87 21 755.5 0 21 88 49 755.5 0 49 89 12 755.5 0 12 90 13 755.5 0 13 91 18 755.5 0 18 92 11 755.5 0 11 93 19 755.5 0 19 94 25 755.5 0 25 95 19 755.5 0 19 96 30 755.5 0 30 97 50 755.5 0 50 98 35 755.5 0 35 99 29 755.5 0 29 100 12 755.5 0 12 101 13 755.5 0 13 102 14 755.5 0 14 103 10 755.5 0 10 104 22 755.5 0 22 105 23 755.5 0 23 106 47 755.5 0 47 107 417 755.5 0 417 108 480 755.5 0 480 109 1120 755.5 0 1120 110 99 755.5 0 99 111 15 755.5 0 15 112 12 755.5 0 12 113 13 755.5 0 13 114 9 755.5 0 9 115 12 755.5 0 12 116 8 755.5 0 8 117 18 755.5 0 18 118 6 755.5 0 6 119 11 755.5 0 11 120 6 755.5 0 6 121 79 755.5 0 79 122 45 755.5 0 45 123 48 755.5 0 48 124 19 755.5 0 19 125 9 755.5 0 9 126 25 755.5 0 25 127 30 755.5 0 30 128 88 755.5 0 88 129 239 755.5 0 239 130 80 755.5 0 80 131 32 755.5 0 32 132 23 755.5 0 23 133 30 755.5 0 30 134 14 755.5 0 14 135 45 755.5 0 45 136 232 755.5 0 232 137 112 755.5 0 112 138 67 755.5 0 67 139 31 755.5 0 31 140 41 755.5 0 41 141 78 755.5 0 78 142 74 755.5 0 74 143 49 755.5 0 49 144 531 755.5 0 531 145 126 755.5 0 126 146 43 755.5 0 43 147 113 755.5 0 113 148 23 755.5 0 23 149 38 755.5 0 38 150 14 755.5 0 14 151 21 755.5 0 21 152 66 755.5 0 66 153 49 755.5 0 49 154 95 755.5 0 95 155 107 755.5 0 107 156 135 755.5 0 135 157 52 755.5 0 52 158 46 755.5 0 46 159 403 755.5 0 403 160 27 755.5 0 27 161 43 755.5 0 43 162 50 755.5 0 50 163 22 755.5 0 22 164 24 755.5 0 24 165 28 755.5 0 28 166 41 755.5 0 41 167 87 755.5 0 87 168 53 755.5 0 53 169 171 755.5 0 171 170 39 755.5 0 39 171 46 755.5 0 46 172 17 755.5 0 17 173 42 755.5 0 42 174 51 755.5 0 51 175 50 755.5 0 50 176 57 755.5 0 57 177 21 755.5 0 21 178 20 755.5 0 20 179 42 755.5 0 42 180 34 755.5 0 34 181 67 755.5 0 67 182 78 755.5 0 78 183 90 755.5 0 90 184 128 755.5 0 128 185 236 755.5 0 236 186 4241 755.5 0 4241 187 765 755.5 0 765 188 1113 755.5 0 1113 189 25 755.5 0 25 190 11 755.5 0 11 191 19 755.5 0 19 192 24 755.5 0 24 193 21 755.5 0 21 194 17 755.5 0 17 195 14 755.5 0 14 196 13 755.5 0 13 197 10 755.5 0 10 198 13 755.5 0 13 199 28 755.5 0 28 200 21 755.5 0 21 201 21 755.5 0 21 202 12 755.5 0 12 203 35 755.5 0 35 204 80 755.5 0 80 205 72 755.5 0 72 206 35 755.5 0 35 207 27 755.5 0 27 208 34 755.5 0 34 209 47 755.5 0 47 210 28 755.5 0 28 211 31 755.5 0 31 212 49 755.5 0 49 213 35 755.5 0 35 214 41 755.5 0 41 215 39 755.5 0 39 216 64 755.5 0 64 217 223 755.5 0 223 218 155 755.5 0 155 219 508 755.5 0 508 220 22 755.5 0 22 221 31 755.5 0 31 222 64 755.5 0 64 223 20 755.5 0 20 224 22 755.5 0 22 225 34 755.5 0 34 226 63 755.5 0 63 227 63 755.5 0 63 228 40 755.5 0 40 229 45 755.5 0 45 230 18 755.5 0 18 231 10 755.5 0 10 232 10 755.5 0 10 233 13 755.5 0 13 234 18 755.5 0 18 235 12 755.5 0 12 236 18 755.5 0 18 237 14 755.5 0 14 238 7 755.5 0 7 239 7 755.5 0 7 240 3 755.5 0 3 241 11 755.5 0 11 242 32 755.5 0 32 243 53 755.5 0 53 244 32 755.5 0 32 245 45 755.5 0 45 246 40 755.5 0 40 247 34 755.5 0 34 248 55 755.5 0 55 249 16 755.5 0 16 250 19 755.5 0 19 251 22 755.5 0 22 252 353 755.5 0 353 253 93 755.5 0 93 254 46 755.5 0 46 255 50 755.5 0 50 256 58 755.5 0 58 257 66 755.5 0 66 258 20 755.5 0 20 259 13 755.5 0 13 260 11 755.5 0 11 261 12 755.5 0 12 262 34 755.5 0 34 263 21 755.5 0 21 264 49 755.5 0 49 265 50 755.5 0 50 266 35 755.5 0 35 267 106 755.5 0 106 268 36 755.5 0 36 269 8 755.5 0 8 270 25 755.5 0 25 271 17 755.5 0 17 272 20 755.5 0 20 273 17 755.5 0 17 274 16 755.5 0 16 275 16 755.5 0 16 276 17 755.5 0 17 277 17 755.5 0 17 278 51 755.5 0 51 279 37 755.5 0 37 280 92 755.5 0 92 281 39 755.5 0 39 282 24 755.5 0 24 283 16 755.5 0 16 284 13 755.5 0 13 285 15 755.5 0 15 286 36 755.5 0 36 287 34 755.5 0 34 288 48 755.5 0 48 289 33 755.5 0 33 290 49 755.5 0 49 291 27 755.5 0 27 292 16 755.5 0 16 293 18 755.5 0 18 294 23 755.5 0 23 295 47 755.5 0 47 296 86 755.5 0 86 297 207 755.5 0 207 298 438 755.5 0 438 299 267 755.5 0 267 300 143 755.5 0 143 301 17 755.5 0 17 Finished in 1.09 s (45 us/read; 1.33 M reads/minute). === Summary === Total reads processed: 24,162 Reads with adapters: 7,341 (30.4%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 7,332 (30.3%) Total basepairs processed: 4,880,081 bp Total written (filtered): 2,008,337 bp (41.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 7341 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 5 0.0 3 0 0 4 0 1 19 74 0.0 3 0 68 4 1 1 20 7227 0.0 4 7044 150 23 6 4 21 11 0.0 4 1 10 22 3 0.0 4 0 0 3 23 5 0.0 4 1 0 0 4 28 1 0.0 4 0 0 0 0 1 48 1 0.0 4 0 0 0 1 97 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 130 3 0.0 4 0 0 0 0 3 136 1 0.0 4 0 0 0 0 1 138 2 0.0 4 0 0 0 0 2 146 1 0.0 4 0 0 0 0 1 167 1 0.0 4 0 0 0 0 1 182 1 0.0 4 0 0 0 0 1 192 1 0.0 4 0 0 0 0 1 238 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.31 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 7,332 Reads with adapters: 590 (8.0%) Reads written (passing filters): 7,332 (100.0%) Total basepairs processed: 2,008,337 bp Total written (filtered): 1,996,561 bp (99.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 590 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.7% C: 2.2% G: 91.7% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 10 1 0.0 1 0 1 11 556 0.0 1 1 555 12 12 0.0 1 0 12 261 17 0.0 1 16 1 262 1 0.0 1 1 269 3 0.0 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.