This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTCATG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 92.73 s (29 us/read; 2.07 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 20,592 (0.6%) Reads that were too short: 4,180 (0.1%) Reads written (passing filters): 16,412 (0.5%) Total basepairs processed: 961,105,341 bp Total written (filtered): 2,824,980 bp (0.3%) === Adapter 1 === Sequence: CTCATG; Type: regular 5'; Length: 6; Trimmed: 20592 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 35 779.6 0 35 7 16 779.6 0 16 8 12 779.6 0 12 9 10 779.6 0 10 10 7 779.6 0 7 11 7 779.6 0 7 12 4 779.6 0 4 13 15 779.6 0 15 14 3 779.6 0 3 15 5 779.6 0 5 16 16 779.6 0 16 17 17 779.6 0 17 18 24 779.6 0 24 19 5 779.6 0 5 20 7 779.6 0 7 21 6 779.6 0 6 22 1 779.6 0 1 23 4 779.6 0 4 24 9 779.6 0 9 25 10 779.6 0 10 26 5 779.6 0 5 27 6 779.6 0 6 28 19 779.6 0 19 29 5 779.6 0 5 30 7 779.6 0 7 31 10 779.6 0 10 32 7 779.6 0 7 33 12 779.6 0 12 34 7 779.6 0 7 35 9 779.6 0 9 36 11 779.6 0 11 37 12 779.6 0 12 38 8 779.6 0 8 39 11 779.6 0 11 40 7 779.6 0 7 41 8 779.6 0 8 42 8 779.6 0 8 43 14 779.6 0 14 44 425 779.6 0 425 45 26 779.6 0 26 46 76 779.6 0 76 47 9 779.6 0 9 48 118 779.6 0 118 49 7 779.6 0 7 50 2 779.6 0 2 51 8 779.6 0 8 52 11 779.6 0 11 53 39 779.6 0 39 54 7 779.6 0 7 55 6 779.6 0 6 56 28 779.6 0 28 57 7 779.6 0 7 58 139 779.6 0 139 59 72 779.6 0 72 60 13 779.6 0 13 61 6 779.6 0 6 62 2 779.6 0 2 63 6 779.6 0 6 64 5 779.6 0 5 65 7 779.6 0 7 66 7 779.6 0 7 67 13 779.6 0 13 68 7 779.6 0 7 69 7 779.6 0 7 70 11 779.6 0 11 71 6 779.6 0 6 72 6 779.6 0 6 73 4 779.6 0 4 74 6 779.6 0 6 75 14 779.6 0 14 76 6 779.6 0 6 77 3 779.6 0 3 78 7 779.6 0 7 79 3 779.6 0 3 80 11 779.6 0 11 81 11 779.6 0 11 82 5 779.6 0 5 83 11 779.6 0 11 84 13 779.6 0 13 85 11 779.6 0 11 86 5 779.6 0 5 87 10 779.6 0 10 88 12 779.6 0 12 89 7 779.6 0 7 90 19 779.6 0 19 91 37 779.6 0 37 92 24 779.6 0 24 93 10 779.6 0 10 94 17 779.6 0 17 95 8 779.6 0 8 96 13 779.6 0 13 97 59 779.6 0 59 98 53 779.6 0 53 99 11 779.6 0 11 100 14 779.6 0 14 101 38 779.6 0 38 102 12 779.6 0 12 103 25 779.6 0 25 104 113 779.6 0 113 105 23 779.6 0 23 106 255 779.6 0 255 107 877 779.6 0 877 108 893 779.6 0 893 109 16 779.6 0 16 110 24 779.6 0 24 111 19 779.6 0 19 112 18 779.6 0 18 113 39 779.6 0 39 114 314 779.6 0 314 115 196 779.6 0 196 116 1243 779.6 0 1243 117 9 779.6 0 9 118 9 779.6 0 9 119 40 779.6 0 40 120 29 779.6 0 29 121 20 779.6 0 20 122 20 779.6 0 20 123 20 779.6 0 20 124 36 779.6 0 36 125 40 779.6 0 40 126 20 779.6 0 20 127 27 779.6 0 27 128 35 779.6 0 35 129 34 779.6 0 34 130 34 779.6 0 34 131 20 779.6 0 20 132 27 779.6 0 27 133 67 779.6 0 67 134 149 779.6 0 149 135 223 779.6 0 223 136 106 779.6 0 106 137 83 779.6 0 83 138 118 779.6 0 118 139 67 779.6 0 67 140 337 779.6 0 337 141 387 779.6 0 387 142 833 779.6 0 833 143 130 779.6 0 130 144 42 779.6 0 42 145 61 779.6 0 61 146 64 779.6 0 64 147 113 779.6 0 113 148 1695 779.6 0 1695 149 2347 779.6 0 2347 150 1317 779.6 0 1317 151 18 779.6 0 18 152 10 779.6 0 10 153 14 779.6 0 14 154 31 779.6 0 31 155 228 779.6 0 228 156 47 779.6 0 47 157 98 779.6 0 98 158 76 779.6 0 76 159 26 779.6 0 26 160 13 779.6 0 13 161 26 779.6 0 26 162 48 779.6 0 48 163 165 779.6 0 165 164 112 779.6 0 112 165 181 779.6 0 181 166 25 779.6 0 25 167 32 779.6 0 32 168 28 779.6 0 28 169 12 779.6 0 12 170 13 779.6 0 13 171 19 779.6 0 19 172 11 779.6 0 11 173 9 779.6 0 9 174 10 779.6 0 10 175 15 779.6 0 15 176 15 779.6 0 15 177 14 779.6 0 14 178 7 779.6 0 7 179 12 779.6 0 12 180 37 779.6 0 37 181 58 779.6 0 58 182 83 779.6 0 83 183 45 779.6 0 45 184 30 779.6 0 30 185 34 779.6 0 34 186 21 779.6 0 21 187 9 779.6 0 9 188 20 779.6 0 20 189 19 779.6 0 19 190 16 779.6 0 16 191 26 779.6 0 26 192 18 779.6 0 18 193 27 779.6 0 27 194 19 779.6 0 19 195 17 779.6 0 17 196 14 779.6 0 14 197 6 779.6 0 6 198 7 779.6 0 7 199 5 779.6 0 5 200 29 779.6 0 29 201 34 779.6 0 34 202 36 779.6 0 36 203 50 779.6 0 50 204 70 779.6 0 70 205 24 779.6 0 24 206 13 779.6 0 13 207 26 779.6 0 26 208 25 779.6 0 25 209 16 779.6 0 16 210 16 779.6 0 16 211 18 779.6 0 18 212 23 779.6 0 23 213 25 779.6 0 25 214 26 779.6 0 26 215 38 779.6 0 38 216 42 779.6 0 42 217 39 779.6 0 39 218 14 779.6 0 14 219 19 779.6 0 19 220 25 779.6 0 25 221 32 779.6 0 32 222 38 779.6 0 38 223 14 779.6 0 14 224 25 779.6 0 25 225 32 779.6 0 32 226 75 779.6 0 75 227 69 779.6 0 69 228 30 779.6 0 30 229 36 779.6 0 36 230 12 779.6 0 12 231 35 779.6 0 35 232 67 779.6 0 67 233 63 779.6 0 63 234 27 779.6 0 27 235 24 779.6 0 24 236 26 779.6 0 26 237 27 779.6 0 27 238 10 779.6 0 10 239 12 779.6 0 12 240 19 779.6 0 19 241 13 779.6 0 13 242 23 779.6 0 23 243 13 779.6 0 13 244 31 779.6 0 31 245 19 779.6 0 19 246 23 779.6 0 23 247 19 779.6 0 19 248 23 779.6 0 23 249 51 779.6 0 51 250 24 779.6 0 24 251 7 779.6 0 7 252 11 779.6 0 11 253 42 779.6 0 42 254 82 779.6 0 82 255 85 779.6 0 85 256 51 779.6 0 51 257 36 779.6 0 36 258 41 779.6 0 41 259 49 779.6 0 49 260 40 779.6 0 40 261 40 779.6 0 40 262 15 779.6 0 15 263 32 779.6 0 32 264 34 779.6 0 34 265 54 779.6 0 54 266 41 779.6 0 41 267 33 779.6 0 33 268 29 779.6 0 29 269 152 779.6 0 152 270 110 779.6 0 110 271 53 779.6 0 53 272 42 779.6 0 42 273 117 779.6 0 117 274 65 779.6 0 65 275 69 779.6 0 69 276 42 779.6 0 42 277 48 779.6 0 48 278 45 779.6 0 45 279 29 779.6 0 29 280 52 779.6 0 52 281 61 779.6 0 61 282 33 779.6 0 33 283 22 779.6 0 22 284 38 779.6 0 38 285 57 779.6 0 57 286 29 779.6 0 29 287 15 779.6 0 15 288 35 779.6 0 35 289 40 779.6 0 40 290 38 779.6 0 38 291 22 779.6 0 22 292 37 779.6 0 37 293 49 779.6 0 49 294 121 779.6 0 121 295 209 779.6 0 209 296 178 779.6 0 178 297 123 779.6 0 123 298 44 779.6 0 44 299 10 779.6 0 10 300 13 779.6 0 13 301 3 779.6 0 3 Finished in 0.62 s (38 us/read; 1.59 M reads/minute). === Summary === Total reads processed: 16,412 Reads with adapters: 60 (0.4%) Reads that were too short: 7 (0.0%) Reads written (passing filters): 53 (0.3%) Total basepairs processed: 2,824,980 bp Total written (filtered): 10,989 bp (0.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 60 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 4 0.0 3 4 18 5 0.0 3 5 19 22 0.0 3 22 43 7 0.0 3 0 0 0 7 46 8 0.0 3 0 0 0 8 68 1 0.0 3 0 0 0 1 69 3 0.0 3 0 0 0 3 70 3 0.0 3 0 0 0 3 132 1 0.0 3 0 0 0 1 145 1 0.0 3 0 0 0 1 165 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 184 2 0.0 3 0 0 0 2 229 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (189 us/read; 0.32 M reads/minute). === Summary === Total reads processed: 53 Reads with adapters: 10 (18.9%) Reads written (passing filters): 53 (100.0%) Total basepairs processed: 10,989 bp Total written (filtered): 10,959 bp (99.7%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 10 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 0.0% C: 20.0% G: 80.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 10 0.8 0 10