This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGACGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 95.55 s (30 us/read; 2.01 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 26,691 (0.8%) Reads that were too short: 21,100 (0.7%) Reads written (passing filters): 5,591 (0.2%) Total basepairs processed: 961,105,341 bp Total written (filtered): 1,099,472 bp (0.1%) === Adapter 1 === Sequence: CGACGT; Type: regular 5'; Length: 6; Trimmed: 26691 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 11 779.6 0 11 7 19 779.6 0 19 8 11 779.6 0 11 9 110 779.6 0 110 10 7 779.6 0 7 11 10 779.6 0 10 12 8 779.6 0 8 13 11 779.6 0 11 14 12 779.6 0 12 15 9 779.6 0 9 16 14 779.6 0 14 17 11 779.6 0 11 18 15 779.6 0 15 19 11 779.6 0 11 20 12 779.6 0 12 21 7 779.6 0 7 22 12 779.6 0 12 23 18 779.6 0 18 24 8 779.6 0 8 25 13 779.6 0 13 26 15 779.6 0 15 27 10 779.6 0 10 28 10 779.6 0 10 29 7 779.6 0 7 30 12 779.6 0 12 31 16 779.6 0 16 32 7 779.6 0 7 33 10 779.6 0 10 34 18 779.6 0 18 35 15 779.6 0 15 36 11 779.6 0 11 37 9 779.6 0 9 38 13 779.6 0 13 39 12 779.6 0 12 40 9 779.6 0 9 41 6 779.6 0 6 42 16 779.6 0 16 43 12 779.6 0 12 44 10 779.6 0 10 45 8 779.6 0 8 46 8 779.6 0 8 47 5 779.6 0 5 48 11 779.6 0 11 49 8 779.6 0 8 50 9 779.6 0 9 51 14 779.6 0 14 52 15 779.6 0 15 53 18 779.6 0 18 54 17 779.6 0 17 55 16 779.6 0 16 56 9 779.6 0 9 57 12 779.6 0 12 58 15 779.6 0 15 59 10 779.6 0 10 60 18 779.6 0 18 61 20 779.6 0 20 62 26 779.6 0 26 63 33 779.6 0 33 64 8 779.6 0 8 65 23 779.6 0 23 66 32 779.6 0 32 67 439 779.6 0 439 68 540 779.6 0 540 69 633 779.6 0 633 70 12 779.6 0 12 71 11 779.6 0 11 72 7 779.6 0 7 73 7 779.6 0 7 74 7 779.6 0 7 75 10 779.6 0 10 76 8 779.6 0 8 77 6 779.6 0 6 78 9 779.6 0 9 79 10 779.6 0 10 80 8 779.6 0 8 81 12 779.6 0 12 82 11 779.6 0 11 83 8 779.6 0 8 84 5 779.6 0 5 85 11 779.6 0 11 86 16 779.6 0 16 87 15 779.6 0 15 88 9 779.6 0 9 89 7 779.6 0 7 90 10 779.6 0 10 91 14 779.6 0 14 92 78 779.6 0 78 93 30 779.6 0 30 94 18 779.6 0 18 95 29 779.6 0 29 96 2 779.6 0 2 97 13 779.6 0 13 98 67 779.6 0 67 99 102 779.6 0 102 100 13 779.6 0 13 101 4 779.6 0 4 102 6 779.6 0 6 103 7 779.6 0 7 104 4 779.6 0 4 105 11 779.6 0 11 106 9 779.6 0 9 107 8 779.6 0 8 108 6 779.6 0 6 109 9 779.6 0 9 110 8 779.6 0 8 111 4 779.6 0 4 112 10 779.6 0 10 113 9 779.6 0 9 114 7 779.6 0 7 115 10 779.6 0 10 116 12 779.6 0 12 117 6 779.6 0 6 118 11 779.6 0 11 119 20 779.6 0 20 120 24 779.6 0 24 121 11 779.6 0 11 122 14 779.6 0 14 123 11 779.6 0 11 124 8 779.6 0 8 125 14 779.6 0 14 126 11 779.6 0 11 127 13 779.6 0 13 128 11 779.6 0 11 129 16 779.6 0 16 130 58 779.6 0 58 131 91 779.6 0 91 132 293 779.6 0 293 133 104 779.6 0 104 134 228 779.6 0 228 135 12 779.6 0 12 136 8 779.6 0 8 137 4 779.6 0 4 138 4 779.6 0 4 139 10 779.6 0 10 140 7 779.6 0 7 141 6 779.6 0 6 142 6 779.6 0 6 143 20 779.6 0 20 144 16 779.6 0 16 145 5 779.6 0 5 146 13 779.6 0 13 147 14 779.6 0 14 148 16 779.6 0 16 149 4 779.6 0 4 150 9 779.6 0 9 151 7 779.6 0 7 152 17 779.6 0 17 153 27 779.6 0 27 154 48 779.6 0 48 155 24 779.6 0 24 156 20 779.6 0 20 157 15 779.6 0 15 158 124 779.6 0 124 159 38 779.6 0 38 160 27 779.6 0 27 161 7 779.6 0 7 162 9 779.6 0 9 163 20 779.6 0 20 164 12 779.6 0 12 165 26 779.6 0 26 166 14 779.6 0 14 167 9 779.6 0 9 168 10 779.6 0 10 169 8 779.6 0 8 170 5 779.6 0 5 171 10 779.6 0 10 172 25 779.6 0 25 173 12 779.6 0 12 174 19 779.6 0 19 175 12 779.6 0 12 176 8 779.6 0 8 177 10 779.6 0 10 178 18 779.6 0 18 179 16 779.6 0 16 180 28 779.6 0 28 181 18 779.6 0 18 182 21 779.6 0 21 183 48 779.6 0 48 184 71 779.6 0 71 185 100 779.6 0 100 186 69 779.6 0 69 187 5 779.6 0 5 188 8 779.6 0 8 189 17 779.6 0 17 190 21 779.6 0 21 191 12 779.6 0 12 192 59 779.6 0 59 193 32 779.6 0 32 194 36 779.6 0 36 195 9 779.6 0 9 196 17 779.6 0 17 197 9 779.6 0 9 198 8 779.6 0 8 199 17 779.6 0 17 200 42 779.6 0 42 201 66 779.6 0 66 202 60 779.6 0 60 203 19 779.6 0 19 204 44 779.6 0 44 205 24 779.6 0 24 206 21 779.6 0 21 207 22 779.6 0 22 208 16 779.6 0 16 209 33 779.6 0 33 210 19 779.6 0 19 211 15 779.6 0 15 212 34 779.6 0 34 213 41 779.6 0 41 214 66 779.6 0 66 215 91 779.6 0 91 216 94 779.6 0 94 217 191 779.6 0 191 218 314 779.6 0 314 219 4490 779.6 0 4490 220 3276 779.6 0 3276 221 5012 779.6 0 5012 222 143 779.6 0 143 223 8 779.6 0 8 224 12 779.6 0 12 225 14 779.6 0 14 226 13 779.6 0 13 227 17 779.6 0 17 228 15 779.6 0 15 229 15 779.6 0 15 230 12 779.6 0 12 231 12 779.6 0 12 232 20 779.6 0 20 233 32 779.6 0 32 234 26 779.6 0 26 235 11 779.6 0 11 236 16 779.6 0 16 237 19 779.6 0 19 238 19 779.6 0 19 239 22 779.6 0 22 240 30 779.6 0 30 241 19 779.6 0 19 242 41 779.6 0 41 243 13 779.6 0 13 244 13 779.6 0 13 245 18 779.6 0 18 246 13 779.6 0 13 247 14 779.6 0 14 248 77 779.6 0 77 249 1457 779.6 0 1457 250 2260 779.6 0 2260 251 1790 779.6 0 1790 252 16 779.6 0 16 253 20 779.6 0 20 254 44 779.6 0 44 255 23 779.6 0 23 256 17 779.6 0 17 257 23 779.6 0 23 258 16 779.6 0 16 259 28 779.6 0 28 260 22 779.6 0 22 261 30 779.6 0 30 262 11 779.6 0 11 263 13 779.6 0 13 264 15 779.6 0 15 265 14 779.6 0 14 266 7 779.6 0 7 267 8 779.6 0 8 268 18 779.6 0 18 269 11 779.6 0 11 270 20 779.6 0 20 271 24 779.6 0 24 272 21 779.6 0 21 273 40 779.6 0 40 274 30 779.6 0 30 275 12 779.6 0 12 276 14 779.6 0 14 277 23 779.6 0 23 278 28 779.6 0 28 279 56 779.6 0 56 280 60 779.6 0 60 281 70 779.6 0 70 282 36 779.6 0 36 283 21 779.6 0 21 284 16 779.6 0 16 285 28 779.6 0 28 286 12 779.6 0 12 287 11 779.6 0 11 288 7 779.6 0 7 289 12 779.6 0 12 290 20 779.6 0 20 291 25 779.6 0 25 292 15 779.6 0 15 293 7 779.6 0 7 294 10 779.6 0 10 295 7 779.6 0 7 296 4 779.6 0 4 297 11 779.6 0 11 298 13 779.6 0 13 299 20 779.6 0 20 300 24 779.6 0 24 301 14 779.6 0 14 Finished in 0.23 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 5,591 Reads with adapters: 28 (0.5%) Reads that were too short: 7 (0.1%) Reads written (passing filters): 21 (0.4%) Total basepairs processed: 1,099,472 bp Total written (filtered): 3,531 bp (0.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 28 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 5 18 1 0.0 3 0 0 1 28 1 0.0 3 0 0 0 1 39 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 109 1 0.0 3 0 0 0 1 116 7 0.0 3 0 0 0 7 122 1 0.0 3 0 0 0 1 125 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 186 1 0.0 3 0 0 0 1 223 4 0.0 3 0 0 0 4 229 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.01 s (476 us/read; 0.13 M reads/minute). === Summary === Total reads processed: 21 Reads with adapters: 1 (4.8%) Reads written (passing filters): 21 (100.0%) Total basepairs processed: 3,531 bp Total written (filtered): 3,528 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 100.0% C: 0.0% G: 0.0% T: 0.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1 0.3 0 1