This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCGGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 96.15 s (30 us/read; 1.99 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 446,463 (14.0%) Reads that were too short: 33,242 (1.0%) Reads written (passing filters): 413,221 (12.9%) Total basepairs processed: 961,105,341 bp Total written (filtered): 85,108,968 bp (8.9%) === Adapter 1 === Sequence: TTCGGA; Type: regular 5'; Length: 6; Trimmed: 446463 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 183 779.6 0 183 7 81 779.6 0 81 8 22 779.6 0 22 9 61 779.6 0 61 10 27 779.6 0 27 11 16 779.6 0 16 12 31 779.6 0 31 13 10 779.6 0 10 14 15 779.6 0 15 15 11 779.6 0 11 16 11 779.6 0 11 17 16 779.6 0 16 18 8 779.6 0 8 19 14 779.6 0 14 20 10 779.6 0 10 21 17 779.6 0 17 22 13 779.6 0 13 23 22 779.6 0 22 24 18 779.6 0 18 25 14 779.6 0 14 26 21 779.6 0 21 27 19 779.6 0 19 28 15 779.6 0 15 29 13 779.6 0 13 30 14 779.6 0 14 31 13 779.6 0 13 32 12 779.6 0 12 33 10 779.6 0 10 34 10 779.6 0 10 35 13 779.6 0 13 36 9 779.6 0 9 37 9 779.6 0 9 38 22 779.6 0 22 39 26 779.6 0 26 40 29 779.6 0 29 41 46 779.6 0 46 42 23 779.6 0 23 43 15 779.6 0 15 44 11 779.6 0 11 45 40 779.6 0 40 46 15 779.6 0 15 47 21 779.6 0 21 48 14 779.6 0 14 49 37 779.6 0 37 50 23 779.6 0 23 51 20 779.6 0 20 52 41 779.6 0 41 53 33 779.6 0 33 54 137 779.6 0 137 55 14 779.6 0 14 56 18 779.6 0 18 57 66 779.6 0 66 58 35 779.6 0 35 59 37 779.6 0 37 60 54 779.6 0 54 61 30 779.6 0 30 62 32 779.6 0 32 63 76 779.6 0 76 64 28 779.6 0 28 65 77 779.6 0 77 66 92 779.6 0 92 67 77 779.6 0 77 68 329 779.6 0 329 69 79 779.6 0 79 70 67 779.6 0 67 71 52 779.6 0 52 72 154 779.6 0 154 73 107 779.6 0 107 74 239 779.6 0 239 75 342 779.6 0 342 76 485 779.6 0 485 77 453 779.6 0 453 78 1072 779.6 0 1072 79 2395 779.6 0 2395 80 543 779.6 0 543 81 768 779.6 0 768 82 1635 779.6 0 1635 83 949 779.6 0 949 84 746 779.6 0 746 85 1901 779.6 0 1901 86 2055 779.6 0 2055 87 1804 779.6 0 1804 88 1570 779.6 0 1570 89 2789 779.6 0 2789 90 2926 779.6 0 2926 91 4590 779.6 0 4590 92 57062 779.6 0 57062 93 158318 779.6 0 158318 94 154651 779.6 0 154651 95 1833 779.6 0 1833 96 96 779.6 0 96 97 14 779.6 0 14 98 15 779.6 0 15 99 7 779.6 0 7 100 8 779.6 0 8 101 5 779.6 0 5 102 7 779.6 0 7 103 10 779.6 0 10 104 8 779.6 0 8 105 14 779.6 0 14 106 9 779.6 0 9 107 75 779.6 0 75 108 23 779.6 0 23 109 23 779.6 0 23 110 75 779.6 0 75 111 37 779.6 0 37 112 135 779.6 0 135 113 11 779.6 0 11 114 47 779.6 0 47 115 18 779.6 0 18 116 31 779.6 0 31 117 6 779.6 0 6 118 10 779.6 0 10 119 3 779.6 0 3 120 5 779.6 0 5 121 4 779.6 0 4 122 4 779.6 0 4 123 6 779.6 0 6 124 1 779.6 0 1 125 14 779.6 0 14 126 7 779.6 0 7 127 9 779.6 0 9 128 7 779.6 0 7 129 6 779.6 0 6 130 15 779.6 0 15 131 10 779.6 0 10 132 6 779.6 0 6 133 7 779.6 0 7 134 7 779.6 0 7 135 11 779.6 0 11 136 15 779.6 0 15 137 19 779.6 0 19 138 11 779.6 0 11 139 74 779.6 0 74 140 45 779.6 0 45 141 151 779.6 0 151 142 4 779.6 0 4 143 13 779.6 0 13 144 9 779.6 0 9 145 10 779.6 0 10 146 4 779.6 0 4 147 7 779.6 0 7 148 13 779.6 0 13 149 5 779.6 0 5 150 10 779.6 0 10 151 15 779.6 0 15 152 8 779.6 0 8 153 4 779.6 0 4 154 7 779.6 0 7 155 10 779.6 0 10 156 10 779.6 0 10 157 33 779.6 0 33 158 17 779.6 0 17 159 13 779.6 0 13 160 2 779.6 0 2 161 8 779.6 0 8 162 5 779.6 0 5 163 9 779.6 0 9 164 4 779.6 0 4 165 12 779.6 0 12 166 8 779.6 0 8 167 11 779.6 0 11 168 4 779.6 0 4 169 15 779.6 0 15 170 80 779.6 0 80 171 29 779.6 0 29 172 16 779.6 0 16 173 20 779.6 0 20 174 14 779.6 0 14 175 26 779.6 0 26 176 34 779.6 0 34 177 80 779.6 0 80 178 288 779.6 0 288 179 421 779.6 0 421 180 542 779.6 0 542 181 19 779.6 0 19 182 9 779.6 0 9 183 21 779.6 0 21 184 27 779.6 0 27 185 50 779.6 0 50 186 55 779.6 0 55 187 131 779.6 0 131 188 216 779.6 0 216 189 77 779.6 0 77 190 355 779.6 0 355 191 244 779.6 0 244 192 338 779.6 0 338 193 71 779.6 0 71 194 107 779.6 0 107 195 102 779.6 0 102 196 129 779.6 0 129 197 133 779.6 0 133 198 128 779.6 0 128 199 172 779.6 0 172 200 381 779.6 0 381 201 5654 779.6 0 5654 202 5426 779.6 0 5426 203 12653 779.6 0 12653 204 497 779.6 0 497 205 794 779.6 0 794 206 701 779.6 0 701 207 637 779.6 0 637 208 266 779.6 0 266 209 89 779.6 0 89 210 40 779.6 0 40 211 29 779.6 0 29 212 35 779.6 0 35 213 34 779.6 0 34 214 23 779.6 0 23 215 79 779.6 0 79 216 105 779.6 0 105 217 181 779.6 0 181 218 957 779.6 0 957 219 38 779.6 0 38 220 30 779.6 0 30 221 81 779.6 0 81 222 50 779.6 0 50 223 302 779.6 0 302 224 101 779.6 0 101 225 439 779.6 0 439 226 550 779.6 0 550 227 1150 779.6 0 1150 228 503 779.6 0 503 229 227 779.6 0 227 230 6 779.6 0 6 231 6 779.6 0 6 232 9 779.6 0 9 233 4 779.6 0 4 234 4 779.6 0 4 235 8 779.6 0 8 236 20 779.6 0 20 237 19 779.6 0 19 238 14 779.6 0 14 239 4 779.6 0 4 240 5 779.6 0 5 241 7 779.6 0 7 242 4 779.6 0 4 243 7 779.6 0 7 244 8 779.6 0 8 245 9 779.6 0 9 246 14 779.6 0 14 247 20 779.6 0 20 248 28 779.6 0 28 249 14 779.6 0 14 250 16 779.6 0 16 251 20 779.6 0 20 252 9 779.6 0 9 253 11 779.6 0 11 254 17 779.6 0 17 255 19 779.6 0 19 256 24 779.6 0 24 257 97 779.6 0 97 258 54 779.6 0 54 259 129 779.6 0 129 260 176 779.6 0 176 261 280 779.6 0 280 262 249 779.6 0 249 263 2838 779.6 0 2838 264 23 779.6 0 23 265 10 779.6 0 10 266 34 779.6 0 34 267 15 779.6 0 15 268 8 779.6 0 8 269 17 779.6 0 17 270 39 779.6 0 39 271 298 779.6 0 298 272 181 779.6 0 181 273 45 779.6 0 45 274 14 779.6 0 14 275 3 779.6 0 3 276 6 779.6 0 6 277 15 779.6 0 15 278 24 779.6 0 24 279 89 779.6 0 89 280 22 779.6 0 22 281 37 779.6 0 37 282 40 779.6 0 40 283 66 779.6 0 66 284 100 779.6 0 100 285 1084 779.6 0 1084 286 445 779.6 0 445 287 297 779.6 0 297 288 9 779.6 0 9 289 2 779.6 0 2 290 1 779.6 0 1 291 8 779.6 0 8 292 40 779.6 0 40 293 14 779.6 0 14 294 30 779.6 0 30 295 28 779.6 0 28 296 2 779.6 0 2 297 10 779.6 0 10 298 7 779.6 0 7 299 6 779.6 0 6 300 2 779.6 0 2 301 5 779.6 0 5 Finished in 16.08 s (39 us/read; 1.54 M reads/minute). === Summary === Total reads processed: 413,221 Reads with adapters: 852 (0.2%) Reads that were too short: 38 (0.0%) Reads written (passing filters): 814 (0.2%) Total basepairs processed: 85,108,968 bp Total written (filtered): 91,180 bp (0.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 852 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 18 1 0.0 3 1 19 14 0.0 3 13 1 44 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 54 1 0.0 3 1 56 1 0.0 3 0 0 0 1 74 3 0.0 3 0 0 0 3 75 2 0.0 3 0 0 0 2 79 11 0.0 3 0 0 0 11 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 82 2 0.0 3 0 0 0 2 98 1 0.0 3 0 0 0 1 99 404 0.0 3 0 0 0 404 100 49 0.0 3 0 0 0 49 101 1 0.0 3 0 0 0 1 102 297 0.0 3 0 0 3 294 103 19 0.0 3 0 0 0 19 105 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 144 1 0.0 3 1 147 2 0.0 3 0 0 0 2 156 1 0.0 3 0 0 0 1 159 2 0.0 3 0 0 0 2 160 2 0.0 3 0 0 0 2 183 2 0.0 3 0 0 1 1 192 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 196 1 0.0 3 0 0 0 1 199 1 0.0 3 0 0 0 1 200 16 0.0 3 0 0 0 16 201 7 0.0 3 0 0 0 7 202 2 0.0 3 0 0 0 2 211 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.02 s (25 us/read; 2.44 M reads/minute). === Summary === Total reads processed: 814 Reads with adapters: 14 (1.7%) Reads written (passing filters): 814 (100.0%) Total basepairs processed: 91,180 bp Total written (filtered): 91,138 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 14 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 7.1% C: 0.0% G: 85.7% T: 7.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 14 12.7 0 14