This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AACCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 91.77 s (29 us/read; 2.09 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 109,625 (3.4%) Reads that were too short: 89,622 (2.8%) Reads written (passing filters): 20,003 (0.6%) Total basepairs processed: 961,105,341 bp Total written (filtered): 3,552,882 bp (0.4%) === Adapter 1 === Sequence: AACCGA; Type: regular 5'; Length: 6; Trimmed: 109625 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 217 779.6 0 217 7 88 779.6 0 88 8 2923 779.6 0 2923 9 23 779.6 0 23 10 5 779.6 0 5 11 9 779.6 0 9 12 25 779.6 0 25 13 3 779.6 0 3 14 11 779.6 0 11 15 3 779.6 0 3 16 7 779.6 0 7 17 6 779.6 0 6 18 8 779.6 0 8 19 5 779.6 0 5 20 9 779.6 0 9 21 7 779.6 0 7 22 8 779.6 0 8 23 6 779.6 0 6 24 8 779.6 0 8 25 10 779.6 0 10 26 5 779.6 0 5 27 5 779.6 0 5 28 5 779.6 0 5 29 5 779.6 0 5 30 6 779.6 0 6 31 9 779.6 0 9 32 5 779.6 0 5 33 4 779.6 0 4 34 1 779.6 0 1 35 8 779.6 0 8 36 8 779.6 0 8 37 7 779.6 0 7 38 10 779.6 0 10 39 21 779.6 0 21 40 3 779.6 0 3 41 4 779.6 0 4 42 21 779.6 0 21 43 11 779.6 0 11 44 9 779.6 0 9 45 7 779.6 0 7 46 8 779.6 0 8 47 5 779.6 0 5 48 7 779.6 0 7 49 9 779.6 0 9 50 8 779.6 0 8 51 11 779.6 0 11 52 9 779.6 0 9 53 9 779.6 0 9 54 3 779.6 0 3 55 8 779.6 0 8 56 10 779.6 0 10 57 5 779.6 0 5 58 7 779.6 0 7 59 6 779.6 0 6 60 7 779.6 0 7 61 4 779.6 0 4 62 11 779.6 0 11 63 4 779.6 0 4 64 7 779.6 0 7 65 7 779.6 0 7 66 6 779.6 0 6 67 7 779.6 0 7 68 14 779.6 0 14 69 7 779.6 0 7 70 13 779.6 0 13 71 21 779.6 0 21 72 25 779.6 0 25 73 7 779.6 0 7 74 5 779.6 0 5 75 9 779.6 0 9 76 22 779.6 0 22 77 34 779.6 0 34 78 130 779.6 0 130 79 56 779.6 0 56 80 95 779.6 0 95 81 93 779.6 0 93 82 73 779.6 0 73 83 114 779.6 0 114 84 83 779.6 0 83 85 205 779.6 0 205 86 55 779.6 0 55 87 17 779.6 0 17 88 51 779.6 0 51 89 44 779.6 0 44 90 37 779.6 0 37 91 16 779.6 0 16 92 32 779.6 0 32 93 107 779.6 0 107 94 284 779.6 0 284 95 483 779.6 0 483 96 77 779.6 0 77 97 22 779.6 0 22 98 56 779.6 0 56 99 87 779.6 0 87 100 119 779.6 0 119 101 72 779.6 0 72 102 67 779.6 0 67 103 30 779.6 0 30 104 41 779.6 0 41 105 182 779.6 0 182 106 34 779.6 0 34 107 21 779.6 0 21 108 37 779.6 0 37 109 44 779.6 0 44 110 62 779.6 0 62 111 52 779.6 0 52 112 40 779.6 0 40 113 44 779.6 0 44 114 68 779.6 0 68 115 146 779.6 0 146 116 67 779.6 0 67 117 84 779.6 0 84 118 125 779.6 0 125 119 136 779.6 0 136 120 120 779.6 0 120 121 83 779.6 0 83 122 65 779.6 0 65 123 103 779.6 0 103 124 91 779.6 0 91 125 171 779.6 0 171 126 646 779.6 0 646 127 218 779.6 0 218 128 168 779.6 0 168 129 61 779.6 0 61 130 57 779.6 0 57 131 109 779.6 0 109 132 76 779.6 0 76 133 108 779.6 0 108 134 141 779.6 0 141 135 156 779.6 0 156 136 165 779.6 0 165 137 118 779.6 0 118 138 150 779.6 0 150 139 115 779.6 0 115 140 123 779.6 0 123 141 171 779.6 0 171 142 66 779.6 0 66 143 60 779.6 0 60 144 140 779.6 0 140 145 210 779.6 0 210 146 60 779.6 0 60 147 68 779.6 0 68 148 40 779.6 0 40 149 57 779.6 0 57 150 69 779.6 0 69 151 46 779.6 0 46 152 80 779.6 0 80 153 171 779.6 0 171 154 177 779.6 0 177 155 216 779.6 0 216 156 153 779.6 0 153 157 115 779.6 0 115 158 185 779.6 0 185 159 101 779.6 0 101 160 146 779.6 0 146 161 53 779.6 0 53 162 73 779.6 0 73 163 64 779.6 0 64 164 131 779.6 0 131 165 162 779.6 0 162 166 105 779.6 0 105 167 71 779.6 0 71 168 89 779.6 0 89 169 186 779.6 0 186 170 65 779.6 0 65 171 77 779.6 0 77 172 136 779.6 0 136 173 320 779.6 0 320 174 367 779.6 0 367 175 214 779.6 0 214 176 92 779.6 0 92 177 94 779.6 0 94 178 144 779.6 0 144 179 175 779.6 0 175 180 248 779.6 0 248 181 206 779.6 0 206 182 142 779.6 0 142 183 131 779.6 0 131 184 88 779.6 0 88 185 137 779.6 0 137 186 88 779.6 0 88 187 43 779.6 0 43 188 35 779.6 0 35 189 130 779.6 0 130 190 394 779.6 0 394 191 463 779.6 0 463 192 159 779.6 0 159 193 121 779.6 0 121 194 60 779.6 0 60 195 63 779.6 0 63 196 182 779.6 0 182 197 1146 779.6 0 1146 198 408 779.6 0 408 199 85 779.6 0 85 200 73 779.6 0 73 201 70 779.6 0 70 202 374 779.6 0 374 203 297 779.6 0 297 204 173 779.6 0 173 205 151 779.6 0 151 206 93 779.6 0 93 207 104 779.6 0 104 208 41 779.6 0 41 209 82 779.6 0 82 210 110 779.6 0 110 211 100 779.6 0 100 212 102 779.6 0 102 213 90 779.6 0 90 214 159 779.6 0 159 215 89 779.6 0 89 216 305 779.6 0 305 217 495 779.6 0 495 218 329 779.6 0 329 219 198 779.6 0 198 220 61 779.6 0 61 221 68 779.6 0 68 222 82 779.6 0 82 223 159 779.6 0 159 224 199 779.6 0 199 225 153 779.6 0 153 226 203 779.6 0 203 227 284 779.6 0 284 228 362 779.6 0 362 229 3221 779.6 0 3221 230 14066 779.6 0 14066 231 4110 779.6 0 4110 232 58 779.6 0 58 233 62 779.6 0 62 234 105 779.6 0 105 235 155 779.6 0 155 236 63 779.6 0 63 237 61 779.6 0 61 238 54 779.6 0 54 239 49 779.6 0 49 240 34 779.6 0 34 241 38 779.6 0 38 242 39 779.6 0 39 243 54 779.6 0 54 244 139 779.6 0 139 245 170 779.6 0 170 246 193 779.6 0 193 247 97 779.6 0 97 248 29 779.6 0 29 249 23 779.6 0 23 250 39 779.6 0 39 251 132 779.6 0 132 252 194 779.6 0 194 253 153 779.6 0 153 254 134 779.6 0 134 255 158 779.6 0 158 256 154 779.6 0 154 257 100 779.6 0 100 258 76 779.6 0 76 259 123 779.6 0 123 260 204 779.6 0 204 261 407 779.6 0 407 262 260 779.6 0 260 263 154 779.6 0 154 264 167 779.6 0 167 265 131 779.6 0 131 266 292 779.6 0 292 267 618 779.6 0 618 268 702 779.6 0 702 269 502 779.6 0 502 270 89 779.6 0 89 271 302 779.6 0 302 272 154 779.6 0 154 273 118 779.6 0 118 274 121 779.6 0 121 275 86 779.6 0 86 276 95 779.6 0 95 277 106 779.6 0 106 278 198 779.6 0 198 279 275 779.6 0 275 280 119 779.6 0 119 281 52 779.6 0 52 282 60 779.6 0 60 283 180 779.6 0 180 284 274 779.6 0 274 285 253 779.6 0 253 286 476 779.6 0 476 287 596 779.6 0 596 288 381 779.6 0 381 289 443 779.6 0 443 290 247 779.6 0 247 291 195 779.6 0 195 292 838 779.6 0 838 293 8099 779.6 0 8099 294 28927 779.6 0 28927 295 13880 779.6 0 13880 296 125 779.6 0 125 297 35 779.6 0 35 298 54 779.6 0 54 299 102 779.6 0 102 300 166 779.6 0 166 301 193 779.6 0 193 Finished in 0.75 s (37 us/read; 1.60 M reads/minute). === Summary === Total reads processed: 20,003 Reads with adapters: 3,205 (16.0%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 3,201 (16.0%) Total basepairs processed: 3,552,882 bp Total written (filtered): 882,344 bp (24.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 3205 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 4 0.0 3 0 0 4 16 35 0.0 3 0 34 0 1 17 2939 0.0 3 2893 44 2 18 10 0.0 3 0 7 2 1 19 9 0.0 3 0 3 5 1 20 195 0.0 3 190 3 0 2 21 1 0.0 3 0 0 0 1 27 1 0.0 3 0 0 0 1 30 1 0.0 3 0 0 0 1 35 1 0.0 3 0 0 0 1 45 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 120 1 0.0 3 0 0 0 1 152 2 0.0 3 0 0 1 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.14 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 3,201 Reads with adapters: 188 (5.9%) Reads written (passing filters): 3,201 (100.0%) Total basepairs processed: 882,344 bp Total written (filtered): 881,765 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 188 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 50.0% C: 20.2% G: 17.0% T: 12.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 176 50.0 0 176 4 11 12.5 0 11 7 1 0.2 0 1