This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AACAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 94.66 s (30 us/read; 2.02 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 363,654 (11.4%) Reads that were too short: 166,231 (5.2%) Reads written (passing filters): 197,423 (6.2%) Total basepairs processed: 961,105,341 bp Total written (filtered): 33,057,704 bp (3.4%) === Adapter 1 === Sequence: AACAAC; Type: regular 5'; Length: 6; Trimmed: 363654 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 33 779.6 0 33 7 781 779.6 0 781 8 27 779.6 0 27 9 18 779.6 0 18 10 29 779.6 0 29 11 14 779.6 0 14 12 17 779.6 0 17 13 8 779.6 0 8 14 12 779.6 0 12 15 18 779.6 0 18 16 13 779.6 0 13 17 10 779.6 0 10 18 8 779.6 0 8 19 18 779.6 0 18 20 6 779.6 0 6 21 15 779.6 0 15 22 12 779.6 0 12 23 15 779.6 0 15 24 13 779.6 0 13 25 14 779.6 0 14 26 12 779.6 0 12 27 16 779.6 0 16 28 5 779.6 0 5 29 8 779.6 0 8 30 16 779.6 0 16 31 10 779.6 0 10 32 9 779.6 0 9 33 14 779.6 0 14 34 9 779.6 0 9 35 19 779.6 0 19 36 16 779.6 0 16 37 14 779.6 0 14 38 17 779.6 0 17 39 18 779.6 0 18 40 11 779.6 0 11 41 19 779.6 0 19 42 11 779.6 0 11 43 27 779.6 0 27 44 21 779.6 0 21 45 11 779.6 0 11 46 12 779.6 0 12 47 16 779.6 0 16 48 6 779.6 0 6 49 13 779.6 0 13 50 21 779.6 0 21 51 9 779.6 0 9 52 7 779.6 0 7 53 19 779.6 0 19 54 18 779.6 0 18 55 15 779.6 0 15 56 13 779.6 0 13 57 16 779.6 0 16 58 11 779.6 0 11 59 17 779.6 0 17 60 13 779.6 0 13 61 14 779.6 0 14 62 7 779.6 0 7 63 10 779.6 0 10 64 11 779.6 0 11 65 43 779.6 0 43 66 82 779.6 0 82 67 115 779.6 0 115 68 16 779.6 0 16 69 36 779.6 0 36 70 55 779.6 0 55 71 59 779.6 0 59 72 52 779.6 0 52 73 106 779.6 0 106 74 73 779.6 0 73 75 81 779.6 0 81 76 558 779.6 0 558 77 1596 779.6 0 1596 78 1557 779.6 0 1557 79 2492 779.6 0 2492 80 2531 779.6 0 2531 81 1681 779.6 0 1681 82 1857 779.6 0 1857 83 3384 779.6 0 3384 84 1824 779.6 0 1824 85 1198 779.6 0 1198 86 1931 779.6 0 1931 87 4955 779.6 0 4955 88 583 779.6 0 583 89 882 779.6 0 882 90 5543 779.6 0 5543 91 2002 779.6 0 2002 92 1413 779.6 0 1413 93 1537 779.6 0 1537 94 2196 779.6 0 2196 95 1064 779.6 0 1064 96 721 779.6 0 721 97 937 779.6 0 937 98 637 779.6 0 637 99 551 779.6 0 551 100 1408 779.6 0 1408 101 1239 779.6 0 1239 102 805 779.6 0 805 103 1776 779.6 0 1776 104 2034 779.6 0 2034 105 858 779.6 0 858 106 1387 779.6 0 1387 107 2318 779.6 0 2318 108 809 779.6 0 809 109 1051 779.6 0 1051 110 1892 779.6 0 1892 111 1727 779.6 0 1727 112 1627 779.6 0 1627 113 1726 779.6 0 1726 114 1257 779.6 0 1257 115 1457 779.6 0 1457 116 3616 779.6 0 3616 117 1091 779.6 0 1091 118 1265 779.6 0 1265 119 2132 779.6 0 2132 120 1619 779.6 0 1619 121 1434 779.6 0 1434 122 2091 779.6 0 2091 123 1391 779.6 0 1391 124 1862 779.6 0 1862 125 1876 779.6 0 1876 126 1762 779.6 0 1762 127 1422 779.6 0 1422 128 2057 779.6 0 2057 129 1302 779.6 0 1302 130 1236 779.6 0 1236 131 2324 779.6 0 2324 132 1701 779.6 0 1701 133 1251 779.6 0 1251 134 1188 779.6 0 1188 135 1218 779.6 0 1218 136 1121 779.6 0 1121 137 1165 779.6 0 1165 138 1192 779.6 0 1192 139 962 779.6 0 962 140 1012 779.6 0 1012 141 1359 779.6 0 1359 142 1939 779.6 0 1939 143 1024 779.6 0 1024 144 1239 779.6 0 1239 145 1802 779.6 0 1802 146 1647 779.6 0 1647 147 1402 779.6 0 1402 148 1211 779.6 0 1211 149 1749 779.6 0 1749 150 2148 779.6 0 2148 151 2575 779.6 0 2575 152 2086 779.6 0 2086 153 2113 779.6 0 2113 154 3013 779.6 0 3013 155 1748 779.6 0 1748 156 1342 779.6 0 1342 157 1596 779.6 0 1596 158 1370 779.6 0 1370 159 1408 779.6 0 1408 160 1286 779.6 0 1286 161 1463 779.6 0 1463 162 1746 779.6 0 1746 163 1400 779.6 0 1400 164 1143 779.6 0 1143 165 1037 779.6 0 1037 166 1017 779.6 0 1017 167 1026 779.6 0 1026 168 1030 779.6 0 1030 169 1088 779.6 0 1088 170 1915 779.6 0 1915 171 1725 779.6 0 1725 172 1009 779.6 0 1009 173 1744 779.6 0 1744 174 1415 779.6 0 1415 175 1262 779.6 0 1262 176 1433 779.6 0 1433 177 1574 779.6 0 1574 178 1954 779.6 0 1954 179 1851 779.6 0 1851 180 1572 779.6 0 1572 181 1361 779.6 0 1361 182 1670 779.6 0 1670 183 1391 779.6 0 1391 184 940 779.6 0 940 185 837 779.6 0 837 186 947 779.6 0 947 187 1358 779.6 0 1358 188 1511 779.6 0 1511 189 1408 779.6 0 1408 190 1449 779.6 0 1449 191 1744 779.6 0 1744 192 1268 779.6 0 1268 193 841 779.6 0 841 194 1530 779.6 0 1530 195 1565 779.6 0 1565 196 767 779.6 0 767 197 1065 779.6 0 1065 198 1347 779.6 0 1347 199 1120 779.6 0 1120 200 1240 779.6 0 1240 201 982 779.6 0 982 202 871 779.6 0 871 203 1361 779.6 0 1361 204 1346 779.6 0 1346 205 786 779.6 0 786 206 809 779.6 0 809 207 999 779.6 0 999 208 789 779.6 0 789 209 931 779.6 0 931 210 970 779.6 0 970 211 986 779.6 0 986 212 1060 779.6 0 1060 213 1418 779.6 0 1418 214 1444 779.6 0 1444 215 1554 779.6 0 1554 216 1013 779.6 0 1013 217 1098 779.6 0 1098 218 1204 779.6 0 1204 219 1189 779.6 0 1189 220 1173 779.6 0 1173 221 1111 779.6 0 1111 222 730 779.6 0 730 223 1323 779.6 0 1323 224 1594 779.6 0 1594 225 1407 779.6 0 1407 226 2236 779.6 0 2236 227 2543 779.6 0 2543 228 3355 779.6 0 3355 229 1276 779.6 0 1276 230 2049 779.6 0 2049 231 3976 779.6 0 3976 232 4458 779.6 0 4458 233 1129 779.6 0 1129 234 1051 779.6 0 1051 235 1525 779.6 0 1525 236 901 779.6 0 901 237 699 779.6 0 699 238 770 779.6 0 770 239 943 779.6 0 943 240 1016 779.6 0 1016 241 1257 779.6 0 1257 242 1078 779.6 0 1078 243 1261 779.6 0 1261 244 1192 779.6 0 1192 245 961 779.6 0 961 246 698 779.6 0 698 247 922 779.6 0 922 248 1001 779.6 0 1001 249 1187 779.6 0 1187 250 1191 779.6 0 1191 251 1381 779.6 0 1381 252 1929 779.6 0 1929 253 1484 779.6 0 1484 254 1071 779.6 0 1071 255 822 779.6 0 822 256 763 779.6 0 763 257 742 779.6 0 742 258 776 779.6 0 776 259 831 779.6 0 831 260 1193 779.6 0 1193 261 1355 779.6 0 1355 262 1889 779.6 0 1889 263 8450 779.6 0 8450 264 11771 779.6 0 11771 265 10194 779.6 0 10194 266 16793 779.6 0 16793 267 965 779.6 0 965 268 1110 779.6 0 1110 269 1047 779.6 0 1047 270 1203 779.6 0 1203 271 1330 779.6 0 1330 272 1181 779.6 0 1181 273 1138 779.6 0 1138 274 1169 779.6 0 1169 275 1280 779.6 0 1280 276 1082 779.6 0 1082 277 1009 779.6 0 1009 278 1214 779.6 0 1214 279 1079 779.6 0 1079 280 1320 779.6 0 1320 281 1097 779.6 0 1097 282 686 779.6 0 686 283 1253 779.6 0 1253 284 1147 779.6 0 1147 285 890 779.6 0 890 286 712 779.6 0 712 287 753 779.6 0 753 288 1088 779.6 0 1088 289 1323 779.6 0 1323 290 1720 779.6 0 1720 291 2691 779.6 0 2691 292 1811 779.6 0 1811 293 1157 779.6 0 1157 294 890 779.6 0 890 295 651 779.6 0 651 296 838 779.6 0 838 297 1033 779.6 0 1033 298 957 779.6 0 957 299 838 779.6 0 838 300 950 779.6 0 950 301 334 779.6 0 334 Finished in 6.60 s (33 us/read; 1.79 M reads/minute). === Summary === Total reads processed: 197,423 Reads with adapters: 837 (0.4%) Reads that were too short: 28 (0.0%) Reads written (passing filters): 809 (0.4%) Total basepairs processed: 33,057,704 bp Total written (filtered): 219,580 bp (0.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 837 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 7 0.0 3 0 5 0 2 17 767 0.0 3 753 13 1 18 6 0.0 3 2 3 1 19 1 0.0 3 0 0 1 20 2 0.0 3 0 0 0 2 23 4 0.0 3 0 0 1 3 24 1 0.0 3 0 0 0 1 25 1 0.0 3 0 0 0 1 32 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 57 1 0.0 3 0 0 0 1 68 5 0.0 3 0 0 0 5 71 5 0.0 3 0 0 0 5 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 105 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 114 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 122 3 0.0 3 0 0 0 3 124 1 0.0 3 0 0 0 1 125 4 0.0 3 0 0 0 4 127 1 0.0 3 0 0 0 1 134 1 0.0 3 0 0 0 1 135 3 0.0 3 0 0 0 3 136 1 0.0 3 0 0 0 1 138 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 154 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 204 1 0.0 3 0 0 0 1 207 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.03 s (37 us/read; 1.62 M reads/minute). === Summary === Total reads processed: 809 Reads with adapters: 124 (15.3%) Reads written (passing filters): 809 (100.0%) Total basepairs processed: 219,580 bp Total written (filtered): 219,208 bp (99.8%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 124 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 9.7% C: 62.1% G: 26.6% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 124 12.6 0 124