This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 104.73 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 135,160 (4.2%) Reads that were too short: 32,270 (1.0%) Reads written (passing filters): 102,890 (3.2%) Total basepairs processed: 961,105,341 bp Total written (filtered): 26,548,499 bp (2.8%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 135160 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1959 779.6 0 1959 7 64891 779.6 0 64891 8 407 779.6 0 407 9 8817 779.6 0 8817 10 35 779.6 0 35 11 7 779.6 0 7 12 5 779.6 0 5 13 7 779.6 0 7 14 5 779.6 0 5 15 2 779.6 0 2 16 11 779.6 0 11 17 7 779.6 0 7 18 4 779.6 0 4 20 3 779.6 0 3 21 8 779.6 0 8 22 16 779.6 0 16 23 16 779.6 0 16 24 1 779.6 0 1 25 4 779.6 0 4 26 3 779.6 0 3 27 2 779.6 0 2 28 6 779.6 0 6 29 8 779.6 0 8 30 8 779.6 0 8 31 5 779.6 0 5 32 3 779.6 0 3 33 4 779.6 0 4 34 5 779.6 0 5 36 3 779.6 0 3 37 5 779.6 0 5 38 8 779.6 0 8 39 2 779.6 0 2 40 11 779.6 0 11 41 108 779.6 0 108 42 30 779.6 0 30 43 7 779.6 0 7 44 1 779.6 0 1 45 5 779.6 0 5 46 5 779.6 0 5 47 4 779.6 0 4 48 8 779.6 0 8 49 3 779.6 0 3 50 4 779.6 0 4 51 8 779.6 0 8 52 5 779.6 0 5 53 24 779.6 0 24 54 14 779.6 0 14 55 6 779.6 0 6 56 10 779.6 0 10 57 6 779.6 0 6 58 5 779.6 0 5 59 2 779.6 0 2 60 9 779.6 0 9 61 4 779.6 0 4 63 7 779.6 0 7 64 4 779.6 0 4 65 8 779.6 0 8 66 13 779.6 0 13 67 23 779.6 0 23 68 24 779.6 0 24 69 223 779.6 0 223 70 481 779.6 0 481 71 456 779.6 0 456 72 2 779.6 0 2 73 10 779.6 0 10 74 3 779.6 0 3 75 18 779.6 0 18 76 26 779.6 0 26 77 9 779.6 0 9 78 29 779.6 0 29 79 22 779.6 0 22 80 107 779.6 0 107 81 44 779.6 0 44 82 52 779.6 0 52 83 32 779.6 0 32 84 71 779.6 0 71 85 76 779.6 0 76 86 102 779.6 0 102 87 101 779.6 0 101 88 130 779.6 0 130 89 180 779.6 0 180 90 42 779.6 0 42 91 107 779.6 0 107 92 52 779.6 0 52 93 30 779.6 0 30 94 58 779.6 0 58 95 42 779.6 0 42 96 51 779.6 0 51 97 72 779.6 0 72 98 37 779.6 0 37 99 193 779.6 0 193 100 620 779.6 0 620 101 479 779.6 0 479 102 790 779.6 0 790 103 258 779.6 0 258 104 84 779.6 0 84 105 41 779.6 0 41 106 22 779.6 0 22 107 34 779.6 0 34 108 35 779.6 0 35 109 29 779.6 0 29 110 15 779.6 0 15 111 32 779.6 0 32 112 60 779.6 0 60 113 60 779.6 0 60 114 40 779.6 0 40 115 57 779.6 0 57 116 64 779.6 0 64 117 105 779.6 0 105 118 103 779.6 0 103 119 157 779.6 0 157 120 113 779.6 0 113 121 147 779.6 0 147 122 109 779.6 0 109 123 196 779.6 0 196 124 176 779.6 0 176 125 126 779.6 0 126 126 144 779.6 0 144 127 75 779.6 0 75 128 79 779.6 0 79 129 44 779.6 0 44 130 103 779.6 0 103 131 137 779.6 0 137 132 230 779.6 0 230 133 294 779.6 0 294 134 277 779.6 0 277 135 178 779.6 0 178 136 112 779.6 0 112 137 151 779.6 0 151 138 143 779.6 0 143 139 143 779.6 0 143 140 140 779.6 0 140 141 114 779.6 0 114 142 202 779.6 0 202 143 1955 779.6 0 1955 144 570 779.6 0 570 145 150 779.6 0 150 146 118 779.6 0 118 147 134 779.6 0 134 148 261 779.6 0 261 149 178 779.6 0 178 150 194 779.6 0 194 151 171 779.6 0 171 152 150 779.6 0 150 153 123 779.6 0 123 154 115 779.6 0 115 155 129 779.6 0 129 156 190 779.6 0 190 157 239 779.6 0 239 158 578 779.6 0 578 159 673 779.6 0 673 160 619 779.6 0 619 161 390 779.6 0 390 162 362 779.6 0 362 163 227 779.6 0 227 164 200 779.6 0 200 165 145 779.6 0 145 166 145 779.6 0 145 167 158 779.6 0 158 168 242 779.6 0 242 169 251 779.6 0 251 170 374 779.6 0 374 171 182 779.6 0 182 172 249 779.6 0 249 173 96 779.6 0 96 174 108 779.6 0 108 175 144 779.6 0 144 176 1419 779.6 0 1419 177 384 779.6 0 384 178 376 779.6 0 376 179 153 779.6 0 153 180 125 779.6 0 125 181 184 779.6 0 184 182 206 779.6 0 206 183 236 779.6 0 236 184 282 779.6 0 282 185 189 779.6 0 189 186 198 779.6 0 198 187 283 779.6 0 283 188 524 779.6 0 524 189 558 779.6 0 558 190 175 779.6 0 175 191 93 779.6 0 93 192 81 779.6 0 81 193 93 779.6 0 93 194 216 779.6 0 216 195 154 779.6 0 154 196 258 779.6 0 258 197 197 779.6 0 197 198 109 779.6 0 109 199 155 779.6 0 155 200 220 779.6 0 220 201 201 779.6 0 201 202 154 779.6 0 154 203 121 779.6 0 121 204 181 779.6 0 181 205 188 779.6 0 188 206 241 779.6 0 241 207 297 779.6 0 297 208 191 779.6 0 191 209 238 779.6 0 238 210 159 779.6 0 159 211 198 779.6 0 198 212 221 779.6 0 221 213 270 779.6 0 270 214 338 779.6 0 338 215 296 779.6 0 296 216 447 779.6 0 447 217 280 779.6 0 280 218 300 779.6 0 300 219 269 779.6 0 269 220 288 779.6 0 288 221 302 779.6 0 302 222 353 779.6 0 353 223 351 779.6 0 351 224 396 779.6 0 396 225 356 779.6 0 356 226 243 779.6 0 243 227 225 779.6 0 225 228 261 779.6 0 261 229 252 779.6 0 252 230 278 779.6 0 278 231 227 779.6 0 227 232 175 779.6 0 175 233 184 779.6 0 184 234 174 779.6 0 174 235 187 779.6 0 187 236 249 779.6 0 249 237 284 779.6 0 284 238 658 779.6 0 658 239 629 779.6 0 629 240 1222 779.6 0 1222 241 326 779.6 0 326 242 296 779.6 0 296 243 342 779.6 0 342 244 395 779.6 0 395 245 365 779.6 0 365 246 379 779.6 0 379 247 336 779.6 0 336 248 477 779.6 0 477 249 579 779.6 0 579 250 338 779.6 0 338 251 279 779.6 0 279 252 287 779.6 0 287 253 570 779.6 0 570 254 552 779.6 0 552 255 674 779.6 0 674 256 205 779.6 0 205 257 183 779.6 0 183 258 192 779.6 0 192 259 231 779.6 0 231 260 188 779.6 0 188 261 164 779.6 0 164 262 306 779.6 0 306 263 263 779.6 0 263 264 299 779.6 0 299 265 215 779.6 0 215 266 181 779.6 0 181 267 246 779.6 0 246 268 374 779.6 0 374 269 426 779.6 0 426 270 404 779.6 0 404 271 366 779.6 0 366 272 244 779.6 0 244 273 279 779.6 0 279 274 313 779.6 0 313 275 413 779.6 0 413 276 614 779.6 0 614 277 531 779.6 0 531 278 501 779.6 0 501 279 221 779.6 0 221 280 368 779.6 0 368 281 372 779.6 0 372 282 515 779.6 0 515 283 526 779.6 0 526 284 318 779.6 0 318 285 244 779.6 0 244 286 141 779.6 0 141 287 156 779.6 0 156 288 269 779.6 0 269 289 352 779.6 0 352 290 324 779.6 0 324 291 328 779.6 0 328 292 260 779.6 0 260 293 277 779.6 0 277 294 301 779.6 0 301 295 355 779.6 0 355 296 269 779.6 0 269 297 146 779.6 0 146 298 186 779.6 0 186 299 152 779.6 0 152 300 205 779.6 0 205 301 969 779.6 0 969 Finished in 5.12 s (50 us/read; 1.21 M reads/minute). === Summary === Total reads processed: 102,890 Reads with adapters: 66,822 (64.9%) Reads that were too short: 26 (0.0%) Reads written (passing filters): 66,796 (64.9%) Total basepairs processed: 26,548,499 bp Total written (filtered): 18,290,624 bp (68.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 66822 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 2 0.0 3 0 0 0 1 1 18 28 0.0 3 0 0 25 2 1 19 706 0.0 3 0 669 21 11 5 20 65541 0.0 4 64125 1243 127 33 13 21 183 0.0 4 23 154 4 0 2 22 23 0.0 4 5 0 17 1 23 193 0.0 4 142 3 0 39 9 24 2 0.0 4 1 0 0 0 1 38 1 0.0 4 1 41 1 0.0 4 0 0 0 0 1 55 2 0.0 4 0 0 0 0 2 60 1 0.0 4 0 0 0 0 1 63 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 1 89 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 100 2 0.0 4 0 0 0 0 2 113 1 0.0 4 0 0 0 0 1 117 1 0.0 4 0 0 0 0 1 119 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 5 0.0 4 0 0 0 0 5 127 104 0.0 4 0 0 0 0 104 130 5 0.0 4 0 0 0 0 5 132 3 0.0 4 0 0 0 0 3 134 1 0.0 4 0 0 0 0 1 153 1 0.0 4 0 0 0 0 1 180 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 202 1 0.0 4 0 0 0 0 1 208 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 282 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.82 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 66,796 Reads with adapters: 2,253 (3.4%) Reads written (passing filters): 66,796 (100.0%) Total basepairs processed: 18,290,624 bp Total written (filtered): 18,262,098 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2253 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 3.3% C: 3.4% G: 91.8% T: 1.5% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 14 1043.7 0 14 4 5 260.9 0 5 9 9 0.3 0 0 9 10 13 0.1 1 0 13 11 2082 0.0 1 9 2073 12 99 0.0 1 4 95 13 1 0.0 1 0 1 15 4 0.0 1 0 4 16 1 0.0 1 0 1 140 1 0.0 1 1 152 19 0.0 1 9 10 191 1 0.0 1 0 1 210 3 0.0 1 2 1 225 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.