This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATGAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 103.49 s (32 us/read; 1.85 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 202,321 (6.3%) Reads that were too short: 52,196 (1.6%) Reads written (passing filters): 150,125 (4.7%) Total basepairs processed: 961,105,341 bp Total written (filtered): 33,087,606 bp (3.4%) === Adapter 1 === Sequence: AATGAA; Type: regular 5'; Length: 6; Trimmed: 202321 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 382 779.6 0 382 7 1028 779.6 0 1028 8 62427 779.6 0 62427 9 137 779.6 0 137 10 15 779.6 0 15 11 13 779.6 0 13 12 8 779.6 0 8 13 14 779.6 0 14 14 13 779.6 0 13 15 11 779.6 0 11 16 4 779.6 0 4 17 10 779.6 0 10 18 8 779.6 0 8 19 10 779.6 0 10 20 11 779.6 0 11 21 10 779.6 0 10 22 12 779.6 0 12 23 17 779.6 0 17 24 5 779.6 0 5 25 15 779.6 0 15 26 14 779.6 0 14 27 14 779.6 0 14 28 13 779.6 0 13 29 8 779.6 0 8 30 14 779.6 0 14 31 13 779.6 0 13 32 15 779.6 0 15 33 8 779.6 0 8 34 14 779.6 0 14 35 16 779.6 0 16 36 13 779.6 0 13 37 19 779.6 0 19 38 17 779.6 0 17 39 11 779.6 0 11 40 6 779.6 0 6 41 15 779.6 0 15 42 10 779.6 0 10 43 11 779.6 0 11 44 13 779.6 0 13 45 25 779.6 0 25 46 20 779.6 0 20 47 39 779.6 0 39 48 12 779.6 0 12 49 17 779.6 0 17 50 13 779.6 0 13 51 11 779.6 0 11 52 19 779.6 0 19 53 15 779.6 0 15 54 17 779.6 0 17 55 20 779.6 0 20 56 18 779.6 0 18 57 10 779.6 0 10 58 8 779.6 0 8 59 22 779.6 0 22 60 17 779.6 0 17 61 14 779.6 0 14 62 19 779.6 0 19 63 29 779.6 0 29 64 32 779.6 0 32 65 54 779.6 0 54 66 98 779.6 0 98 67 29 779.6 0 29 68 45 779.6 0 45 69 161 779.6 0 161 70 40 779.6 0 40 71 51 779.6 0 51 72 46 779.6 0 46 73 105 779.6 0 105 74 34 779.6 0 34 75 58 779.6 0 58 76 90 779.6 0 90 77 469 779.6 0 469 78 296 779.6 0 296 79 2333 779.6 0 2333 80 1290 779.6 0 1290 81 5559 779.6 0 5559 82 625 779.6 0 625 83 642 779.6 0 642 84 951 779.6 0 951 85 366 779.6 0 366 86 757 779.6 0 757 87 77 779.6 0 77 88 47 779.6 0 47 89 324 779.6 0 324 90 567 779.6 0 567 91 46 779.6 0 46 92 56 779.6 0 56 93 348 779.6 0 348 94 1273 779.6 0 1273 95 1055 779.6 0 1055 96 1229 779.6 0 1229 97 162 779.6 0 162 98 66 779.6 0 66 99 505 779.6 0 505 100 2872 779.6 0 2872 101 1262 779.6 0 1262 102 352 779.6 0 352 103 246 779.6 0 246 104 96 779.6 0 96 105 242 779.6 0 242 106 939 779.6 0 939 107 173 779.6 0 173 108 109 779.6 0 109 109 224 779.6 0 224 110 445 779.6 0 445 111 348 779.6 0 348 112 380 779.6 0 380 113 253 779.6 0 253 114 253 779.6 0 253 115 632 779.6 0 632 116 503 779.6 0 503 117 464 779.6 0 464 118 1095 779.6 0 1095 119 849 779.6 0 849 120 571 779.6 0 571 121 504 779.6 0 504 122 425 779.6 0 425 123 370 779.6 0 370 124 569 779.6 0 569 125 894 779.6 0 894 126 1220 779.6 0 1220 127 1441 779.6 0 1441 128 322 779.6 0 322 129 355 779.6 0 355 130 685 779.6 0 685 131 959 779.6 0 959 132 702 779.6 0 702 133 461 779.6 0 461 134 467 779.6 0 467 135 511 779.6 0 511 136 900 779.6 0 900 137 1093 779.6 0 1093 138 820 779.6 0 820 139 733 779.6 0 733 140 617 779.6 0 617 141 1347 779.6 0 1347 142 436 779.6 0 436 143 717 779.6 0 717 144 522 779.6 0 522 145 1003 779.6 0 1003 146 617 779.6 0 617 147 463 779.6 0 463 148 376 779.6 0 376 149 439 779.6 0 439 150 410 779.6 0 410 151 333 779.6 0 333 152 492 779.6 0 492 153 470 779.6 0 470 154 656 779.6 0 656 155 378 779.6 0 378 156 405 779.6 0 405 157 880 779.6 0 880 158 1200 779.6 0 1200 159 1150 779.6 0 1150 160 981 779.6 0 981 161 426 779.6 0 426 162 446 779.6 0 446 163 444 779.6 0 444 164 545 779.6 0 545 165 614 779.6 0 614 166 1021 779.6 0 1021 167 393 779.6 0 393 168 434 779.6 0 434 169 731 779.6 0 731 170 780 779.6 0 780 171 297 779.6 0 297 172 267 779.6 0 267 173 598 779.6 0 598 174 787 779.6 0 787 175 467 779.6 0 467 176 302 779.6 0 302 177 222 779.6 0 222 178 310 779.6 0 310 179 687 779.6 0 687 180 748 779.6 0 748 181 655 779.6 0 655 182 1257 779.6 0 1257 183 1670 779.6 0 1670 184 1877 779.6 0 1877 185 752 779.6 0 752 186 682 779.6 0 682 187 473 779.6 0 473 188 212 779.6 0 212 189 204 779.6 0 204 190 383 779.6 0 383 191 780 779.6 0 780 192 847 779.6 0 847 193 732 779.6 0 732 194 535 779.6 0 535 195 260 779.6 0 260 196 218 779.6 0 218 197 811 779.6 0 811 198 1750 779.6 0 1750 199 845 779.6 0 845 200 263 779.6 0 263 201 144 779.6 0 144 202 252 779.6 0 252 203 665 779.6 0 665 204 902 779.6 0 902 205 784 779.6 0 784 206 717 779.6 0 717 207 456 779.6 0 456 208 288 779.6 0 288 209 387 779.6 0 387 210 520 779.6 0 520 211 671 779.6 0 671 212 568 779.6 0 568 213 486 779.6 0 486 214 448 779.6 0 448 215 418 779.6 0 418 216 359 779.6 0 359 217 556 779.6 0 556 218 630 779.6 0 630 219 693 779.6 0 693 220 394 779.6 0 394 221 204 779.6 0 204 222 305 779.6 0 305 223 636 779.6 0 636 224 565 779.6 0 565 225 383 779.6 0 383 226 482 779.6 0 482 227 539 779.6 0 539 228 510 779.6 0 510 229 472 779.6 0 472 230 538 779.6 0 538 231 413 779.6 0 413 232 208 779.6 0 208 233 226 779.6 0 226 234 638 779.6 0 638 235 901 779.6 0 901 236 558 779.6 0 558 237 640 779.6 0 640 238 631 779.6 0 631 239 667 779.6 0 667 240 329 779.6 0 329 241 291 779.6 0 291 242 383 779.6 0 383 243 404 779.6 0 404 244 479 779.6 0 479 245 422 779.6 0 422 246 617 779.6 0 617 247 593 779.6 0 593 248 453 779.6 0 453 249 224 779.6 0 224 250 224 779.6 0 224 251 924 779.6 0 924 252 911 779.6 0 911 253 706 779.6 0 706 254 988 779.6 0 988 255 1160 779.6 0 1160 256 1081 779.6 0 1081 257 505 779.6 0 505 258 359 779.6 0 359 259 263 779.6 0 263 260 245 779.6 0 245 261 317 779.6 0 317 262 433 779.6 0 433 263 452 779.6 0 452 264 418 779.6 0 418 265 382 779.6 0 382 266 485 779.6 0 485 267 597 779.6 0 597 268 475 779.6 0 475 269 232 779.6 0 232 270 239 779.6 0 239 271 533 779.6 0 533 272 608 779.6 0 608 273 756 779.6 0 756 274 711 779.6 0 711 275 465 779.6 0 465 276 263 779.6 0 263 277 292 779.6 0 292 278 539 779.6 0 539 279 692 779.6 0 692 280 538 779.6 0 538 281 240 779.6 0 240 282 188 779.6 0 188 283 289 779.6 0 289 284 421 779.6 0 421 285 441 779.6 0 441 286 623 779.6 0 623 287 470 779.6 0 470 288 408 779.6 0 408 289 353 779.6 0 353 290 338 779.6 0 338 291 476 779.6 0 476 292 761 779.6 0 761 293 956 779.6 0 956 294 612 779.6 0 612 295 281 779.6 0 281 296 522 779.6 0 522 297 651 779.6 0 651 298 472 779.6 0 472 299 470 779.6 0 470 300 1028 779.6 0 1028 301 1498 779.6 0 1498 Finished in 6.35 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 150,125 Reads with adapters: 63,860 (42.5%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 63,848 (42.5%) Total basepairs processed: 33,087,606 bp Total written (filtered): 17,609,064 bp (53.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 63860 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 2 0.0 2 0 0 0 2 15 22 0.0 3 0 0 19 3 16 434 0.0 3 0 430 4 17 62954 0.0 3 62129 795 16 14 18 157 0.0 3 15 134 5 3 19 130 0.0 3 3 0 115 12 20 66 0.0 3 0 0 0 66 21 13 0.0 3 0 0 0 13 25 5 0.0 3 1 0 0 4 28 5 0.0 3 0 0 0 5 41 1 0.0 3 0 0 0 1 42 2 0.0 3 0 0 0 2 43 1 0.0 3 0 0 0 1 44 6 0.0 3 0 0 0 6 46 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 51 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 53 1 0.0 3 0 0 0 1 58 1 0.0 3 0 0 1 60 1 0.0 3 0 0 0 1 63 3 0.0 3 0 0 0 3 65 18 0.0 3 0 0 0 18 67 3 0.0 3 0 0 0 3 74 1 0.0 3 0 0 0 1 75 3 0.0 3 0 0 0 3 76 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 106 2 0.0 3 0 0 0 2 109 2 0.0 3 0 0 0 2 112 3 0.0 3 0 0 0 3 115 4 0.0 3 0 0 0 4 117 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 170 1 0.0 3 0 0 0 1 173 1 0.0 3 0 0 0 1 187 1 0.0 3 0 0 0 1 202 1 0.0 3 0 0 0 1 206 1 0.0 3 0 0 0 1 214 4 0.0 3 0 0 0 4 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.91 s (46 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 63,848 Reads with adapters: 6,258 (9.8%) Reads written (passing filters): 63,848 (100.0%) Total basepairs processed: 17,609,064 bp Total written (filtered): 17,590,218 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 6258 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 14.1% C: 3.8% G: 81.4% T: 0.7% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 6186 997.6 0 6186 4 72 249.4 0 72 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.