This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGAAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 102.00 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 164,794 (5.2%) Reads that were too short: 62,194 (1.9%) Reads written (passing filters): 102,600 (3.2%) Total basepairs processed: 961,105,341 bp Total written (filtered): 29,148,182 bp (3.0%) === Adapter 1 === Sequence: TGGAAC; Type: regular 5'; Length: 6; Trimmed: 164794 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 9492 779.6 0 9492 7 1426 779.6 0 1426 8 64742 779.6 0 64742 9 19647 779.6 0 19647 10 55 779.6 0 55 11 18 779.6 0 18 12 16 779.6 0 16 13 13 779.6 0 13 14 24 779.6 0 24 15 8 779.6 0 8 16 8 779.6 0 8 17 26 779.6 0 26 18 16 779.6 0 16 19 18 779.6 0 18 20 15 779.6 0 15 21 22 779.6 0 22 22 16 779.6 0 16 23 28 779.6 0 28 24 21 779.6 0 21 25 18 779.6 0 18 26 7 779.6 0 7 27 7 779.6 0 7 28 14 779.6 0 14 29 9 779.6 0 9 30 14 779.6 0 14 31 13 779.6 0 13 32 18 779.6 0 18 33 12 779.6 0 12 34 13 779.6 0 13 35 9 779.6 0 9 36 8 779.6 0 8 37 13 779.6 0 13 38 15 779.6 0 15 39 10 779.6 0 10 40 12 779.6 0 12 41 7 779.6 0 7 42 15 779.6 0 15 43 15 779.6 0 15 44 12 779.6 0 12 45 9 779.6 0 9 46 12 779.6 0 12 47 8 779.6 0 8 48 7 779.6 0 7 49 8 779.6 0 8 50 12 779.6 0 12 51 63 779.6 0 63 52 27 779.6 0 27 53 74 779.6 0 74 54 11 779.6 0 11 55 12 779.6 0 12 56 11 779.6 0 11 57 9 779.6 0 9 58 11 779.6 0 11 59 14 779.6 0 14 60 10 779.6 0 10 61 13 779.6 0 13 62 25 779.6 0 25 63 19 779.6 0 19 64 14 779.6 0 14 65 12 779.6 0 12 66 12 779.6 0 12 67 13 779.6 0 13 68 18 779.6 0 18 69 13 779.6 0 13 70 9 779.6 0 9 71 12 779.6 0 12 72 12 779.6 0 12 73 17 779.6 0 17 74 38 779.6 0 38 75 14 779.6 0 14 76 12 779.6 0 12 77 4 779.6 0 4 78 11 779.6 0 11 79 15 779.6 0 15 80 16 779.6 0 16 81 10 779.6 0 10 82 36 779.6 0 36 83 28 779.6 0 28 84 31 779.6 0 31 85 32 779.6 0 32 86 59 779.6 0 59 87 140 779.6 0 140 88 17 779.6 0 17 89 22 779.6 0 22 90 18 779.6 0 18 91 23 779.6 0 23 92 14 779.6 0 14 93 29 779.6 0 29 94 23 779.6 0 23 95 35 779.6 0 35 96 64 779.6 0 64 97 56 779.6 0 56 98 13 779.6 0 13 99 15 779.6 0 15 100 21 779.6 0 21 101 23 779.6 0 23 102 42 779.6 0 42 103 36 779.6 0 36 104 18 779.6 0 18 105 16 779.6 0 16 106 10 779.6 0 10 107 11 779.6 0 11 108 17 779.6 0 17 109 11 779.6 0 11 110 9 779.6 0 9 111 15 779.6 0 15 112 23 779.6 0 23 113 22 779.6 0 22 114 20 779.6 0 20 115 20 779.6 0 20 116 56 779.6 0 56 117 93 779.6 0 93 118 83 779.6 0 83 119 78 779.6 0 78 120 32 779.6 0 32 121 27 779.6 0 27 122 26 779.6 0 26 123 36 779.6 0 36 124 26 779.6 0 26 125 61 779.6 0 61 126 43 779.6 0 43 127 26 779.6 0 26 128 28 779.6 0 28 129 15 779.6 0 15 130 27 779.6 0 27 131 21 779.6 0 21 132 39 779.6 0 39 133 42 779.6 0 42 134 34 779.6 0 34 135 35 779.6 0 35 136 46 779.6 0 46 137 94 779.6 0 94 138 280 779.6 0 280 139 408 779.6 0 408 140 280 779.6 0 280 141 28 779.6 0 28 142 60 779.6 0 60 143 48 779.6 0 48 144 42 779.6 0 42 145 22 779.6 0 22 146 48 779.6 0 48 147 24 779.6 0 24 148 30 779.6 0 30 149 26 779.6 0 26 150 27 779.6 0 27 151 25 779.6 0 25 152 28 779.6 0 28 153 20 779.6 0 20 154 17 779.6 0 17 155 33 779.6 0 33 156 37 779.6 0 37 157 30 779.6 0 30 158 41 779.6 0 41 159 33 779.6 0 33 160 46 779.6 0 46 161 37 779.6 0 37 162 23 779.6 0 23 163 16 779.6 0 16 164 29 779.6 0 29 165 26 779.6 0 26 166 33 779.6 0 33 167 33 779.6 0 33 168 29 779.6 0 29 169 43 779.6 0 43 170 60 779.6 0 60 171 53 779.6 0 53 172 32 779.6 0 32 173 21 779.6 0 21 174 39 779.6 0 39 175 43 779.6 0 43 176 30 779.6 0 30 177 27 779.6 0 27 178 26 779.6 0 26 179 57 779.6 0 57 180 106 779.6 0 106 181 36 779.6 0 36 182 52 779.6 0 52 183 43 779.6 0 43 184 28 779.6 0 28 185 30 779.6 0 30 186 122 779.6 0 122 187 454 779.6 0 454 188 471 779.6 0 471 189 109 779.6 0 109 190 11 779.6 0 11 191 15 779.6 0 15 192 21 779.6 0 21 193 24 779.6 0 24 194 45 779.6 0 45 195 83 779.6 0 83 196 27 779.6 0 27 197 35 779.6 0 35 198 27 779.6 0 27 199 59 779.6 0 59 200 71 779.6 0 71 201 45 779.6 0 45 202 15 779.6 0 15 203 15 779.6 0 15 204 27 779.6 0 27 205 43 779.6 0 43 206 50 779.6 0 50 207 114 779.6 0 114 208 33 779.6 0 33 209 23 779.6 0 23 210 22 779.6 0 22 211 28 779.6 0 28 212 42 779.6 0 42 213 35 779.6 0 35 214 99 779.6 0 99 215 209 779.6 0 209 216 131 779.6 0 131 217 28 779.6 0 28 218 44 779.6 0 44 219 47 779.6 0 47 220 65 779.6 0 65 221 44 779.6 0 44 222 20 779.6 0 20 223 13 779.6 0 13 224 21 779.6 0 21 225 46 779.6 0 46 226 45 779.6 0 45 227 75 779.6 0 75 228 69 779.6 0 69 229 26 779.6 0 26 230 55 779.6 0 55 231 38 779.6 0 38 232 33 779.6 0 33 233 24 779.6 0 24 234 12 779.6 0 12 235 33 779.6 0 33 236 43 779.6 0 43 237 46 779.6 0 46 238 55 779.6 0 55 239 58 779.6 0 58 240 58 779.6 0 58 241 29 779.6 0 29 242 17 779.6 0 17 243 28 779.6 0 28 244 22 779.6 0 22 245 20 779.6 0 20 246 32 779.6 0 32 247 49 779.6 0 49 248 41 779.6 0 41 249 51 779.6 0 51 250 35 779.6 0 35 251 40 779.6 0 40 252 38 779.6 0 38 253 32 779.6 0 32 254 31 779.6 0 31 255 22 779.6 0 22 256 25 779.6 0 25 257 44 779.6 0 44 258 98 779.6 0 98 259 124 779.6 0 124 260 220 779.6 0 220 261 166 779.6 0 166 262 276 779.6 0 276 263 100 779.6 0 100 264 38 779.6 0 38 265 37 779.6 0 37 266 52 779.6 0 52 267 77 779.6 0 77 268 112 779.6 0 112 269 152 779.6 0 152 270 83 779.6 0 83 271 128 779.6 0 128 272 143 779.6 0 143 273 1531 779.6 0 1531 274 2550 779.6 0 2550 275 4445 779.6 0 4445 276 270 779.6 0 270 277 175 779.6 0 175 278 154 779.6 0 154 279 144 779.6 0 144 280 229 779.6 0 229 281 305 779.6 0 305 282 205 779.6 0 205 283 212 779.6 0 212 284 156 779.6 0 156 285 249 779.6 0 249 286 315 779.6 0 315 287 278 779.6 0 278 288 548 779.6 0 548 289 241 779.6 0 241 290 695 779.6 0 695 291 311 779.6 0 311 292 248 779.6 0 248 293 2203 779.6 0 2203 294 2601 779.6 0 2601 295 2447 779.6 0 2447 296 1087 779.6 0 1087 297 1724 779.6 0 1724 298 3695 779.6 0 3695 299 8052 779.6 0 8052 300 12458 779.6 0 12458 301 10420 779.6 0 10420 Finished in 6.20 s (60 us/read; 0.99 M reads/minute). === Summary === Total reads processed: 102,600 Reads with adapters: 29,197 (28.5%) Reads that were too short: 31 (0.0%) Reads written (passing filters): 29,166 (28.4%) Total basepairs processed: 29,148,182 bp Total written (filtered): 7,924,085 bp (27.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 29197 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 10 0.0 3 0 0 9 0 1 19 291 0.0 3 0 270 12 6 3 20 20151 0.0 4 19502 554 67 20 8 21 147 0.0 4 8 127 8 2 2 22 8332 0.0 4 8041 246 37 3 5 23 37 0.0 4 9 22 1 4 1 24 4 0.0 4 0 0 1 0 3 25 9 0.0 4 0 0 1 7 1 26 1 0.0 4 0 0 0 0 1 29 1 0.0 4 1 40 1 0.0 4 1 49 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 73 1 0.0 4 0 1 83 1 0.0 4 0 0 0 0 1 88 2 0.0 4 2 98 1 0.0 4 0 0 0 0 1 126 1 0.0 4 0 0 0 0 1 127 169 0.0 4 0 0 0 0 169 128 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 147 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 153 4 0.0 4 0 0 0 0 4 183 1 0.0 4 0 0 0 0 1 228 6 0.0 4 0 0 0 0 6 235 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 258 5 0.0 4 0 0 0 0 5 260 9 0.0 4 0 0 0 0 9 265 1 0.0 4 0 0 0 0 1 290 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 1.27 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 29,166 Reads with adapters: 5,496 (18.8%) Reads written (passing filters): 29,166 (100.0%) Total basepairs processed: 7,924,085 bp Total written (filtered): 7,245,476 bp (91.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 5496 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 1.2% C: 1.1% G: 53.8% T: 0.9% none/other: 43.0% Overview of removed sequences length count expect max.err error counts 3 3 455.7 0 3 6 1 7.1 0 1 9 98 0.1 0 9 89 10 355 0.0 1 11 344 11 2660 0.0 1 9 2651 12 1 0.0 1 0 1 13 6 0.0 1 0 6 151 1 0.0 1 0 1 208 2 0.0 1 2 209 3 0.0 1 2 1 211 1 0.0 1 1 260 1 0.0 1 1 264 1 0.0 1 0 1 270 1 0.0 1 1 271 6 0.0 1 6 272 2252 0.0 1 2135 117 273 69 0.0 1 65 4 274 20 0.0 1 20 275 14 0.0 1 14 276 1 0.0 1 1