This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTATAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 104.96 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 101,209 (3.2%) Reads that were too short: 39,631 (1.2%) Reads written (passing filters): 61,578 (1.9%) Total basepairs processed: 961,105,341 bp Total written (filtered): 12,282,331 bp (1.3%) === Adapter 1 === Sequence: CTATAA; Type: regular 5'; Length: 6; Trimmed: 101209 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 472 779.6 0 472 7 18456 779.6 0 18456 8 25 779.6 0 25 9 6 779.6 0 6 10 7 779.6 0 7 11 4 779.6 0 4 12 3 779.6 0 3 13 3 779.6 0 3 14 2 779.6 0 2 15 5 779.6 0 5 16 13 779.6 0 13 17 11 779.6 0 11 18 15 779.6 0 15 19 4 779.6 0 4 20 5 779.6 0 5 21 3 779.6 0 3 22 4 779.6 0 4 23 5 779.6 0 5 24 3 779.6 0 3 25 3 779.6 0 3 26 61 779.6 0 61 27 26 779.6 0 26 28 46 779.6 0 46 29 5 779.6 0 5 30 5 779.6 0 5 31 2 779.6 0 2 32 6 779.6 0 6 33 2 779.6 0 2 34 3 779.6 0 3 35 2 779.6 0 2 36 3 779.6 0 3 37 9 779.6 0 9 38 4 779.6 0 4 39 2 779.6 0 2 40 8 779.6 0 8 41 6 779.6 0 6 42 11 779.6 0 11 43 1 779.6 0 1 44 3 779.6 0 3 45 4 779.6 0 4 47 1 779.6 0 1 48 3 779.6 0 3 49 4 779.6 0 4 50 3 779.6 0 3 51 6 779.6 0 6 52 3 779.6 0 3 53 6 779.6 0 6 54 4 779.6 0 4 55 4 779.6 0 4 56 4 779.6 0 4 57 2 779.6 0 2 58 3 779.6 0 3 59 3 779.6 0 3 60 3 779.6 0 3 61 5 779.6 0 5 62 3 779.6 0 3 63 3 779.6 0 3 64 9 779.6 0 9 65 6 779.6 0 6 66 7 779.6 0 7 67 1 779.6 0 1 68 6 779.6 0 6 69 13 779.6 0 13 71 13 779.6 0 13 72 7 779.6 0 7 73 2 779.6 0 2 74 5 779.6 0 5 75 5 779.6 0 5 76 2 779.6 0 2 77 5 779.6 0 5 78 11 779.6 0 11 79 34 779.6 0 34 80 64 779.6 0 64 81 90 779.6 0 90 82 143 779.6 0 143 83 116 779.6 0 116 84 112 779.6 0 112 85 218 779.6 0 218 86 106 779.6 0 106 87 27 779.6 0 27 88 73 779.6 0 73 89 165 779.6 0 165 90 25 779.6 0 25 91 125 779.6 0 125 92 79 779.6 0 79 93 62 779.6 0 62 94 72 779.6 0 72 95 423 779.6 0 423 96 644 779.6 0 644 97 295 779.6 0 295 98 161 779.6 0 161 99 206 779.6 0 206 100 107 779.6 0 107 101 192 779.6 0 192 102 327 779.6 0 327 103 173 779.6 0 173 104 102 779.6 0 102 105 486 779.6 0 486 106 511 779.6 0 511 107 446 779.6 0 446 108 1523 779.6 0 1523 109 845 779.6 0 845 110 438 779.6 0 438 111 321 779.6 0 321 112 780 779.6 0 780 113 374 779.6 0 374 114 1253 779.6 0 1253 115 796 779.6 0 796 116 228 779.6 0 228 117 194 779.6 0 194 118 108 779.6 0 108 119 77 779.6 0 77 120 104 779.6 0 104 121 167 779.6 0 167 122 439 779.6 0 439 123 309 779.6 0 309 124 423 779.6 0 423 125 157 779.6 0 157 126 273 779.6 0 273 127 277 779.6 0 277 128 196 779.6 0 196 129 447 779.6 0 447 130 301 779.6 0 301 131 354 779.6 0 354 132 418 779.6 0 418 133 248 779.6 0 248 134 259 779.6 0 259 135 291 779.6 0 291 136 454 779.6 0 454 137 615 779.6 0 615 138 458 779.6 0 458 139 314 779.6 0 314 140 297 779.6 0 297 141 712 779.6 0 712 142 426 779.6 0 426 143 268 779.6 0 268 144 168 779.6 0 168 145 168 779.6 0 168 146 316 779.6 0 316 147 334 779.6 0 334 148 1133 779.6 0 1133 149 417 779.6 0 417 150 622 779.6 0 622 151 203 779.6 0 203 152 225 779.6 0 225 153 416 779.6 0 416 154 222 779.6 0 222 155 493 779.6 0 493 156 432 779.6 0 432 157 447 779.6 0 447 158 442 779.6 0 442 159 520 779.6 0 520 160 261 779.6 0 261 161 300 779.6 0 300 162 217 779.6 0 217 163 641 779.6 0 641 164 392 779.6 0 392 165 535 779.6 0 535 166 365 779.6 0 365 167 354 779.6 0 354 168 335 779.6 0 335 169 406 779.6 0 406 170 296 779.6 0 296 171 140 779.6 0 140 172 252 779.6 0 252 173 150 779.6 0 150 174 342 779.6 0 342 175 386 779.6 0 386 176 602 779.6 0 602 177 246 779.6 0 246 178 180 779.6 0 180 179 145 779.6 0 145 180 153 779.6 0 153 181 304 779.6 0 304 182 390 779.6 0 390 183 370 779.6 0 370 184 278 779.6 0 278 185 367 779.6 0 367 186 575 779.6 0 575 187 511 779.6 0 511 188 325 779.6 0 325 189 152 779.6 0 152 190 220 779.6 0 220 191 229 779.6 0 229 192 540 779.6 0 540 193 559 779.6 0 559 194 839 779.6 0 839 195 244 779.6 0 244 196 360 779.6 0 360 197 157 779.6 0 157 198 199 779.6 0 199 199 427 779.6 0 427 200 538 779.6 0 538 201 600 779.6 0 600 202 614 779.6 0 614 203 211 779.6 0 211 204 210 779.6 0 210 205 399 779.6 0 399 206 460 779.6 0 460 207 250 779.6 0 250 208 735 779.6 0 735 209 562 779.6 0 562 210 495 779.6 0 495 211 369 779.6 0 369 212 239 779.6 0 239 213 280 779.6 0 280 214 273 779.6 0 273 215 231 779.6 0 231 216 289 779.6 0 289 217 238 779.6 0 238 218 358 779.6 0 358 219 531 779.6 0 531 220 336 779.6 0 336 221 276 779.6 0 276 222 463 779.6 0 463 223 235 779.6 0 235 224 186 779.6 0 186 225 228 779.6 0 228 226 302 779.6 0 302 227 376 779.6 0 376 228 411 779.6 0 411 229 440 779.6 0 440 230 614 779.6 0 614 231 405 779.6 0 405 232 248 779.6 0 248 233 214 779.6 0 214 234 176 779.6 0 176 235 251 779.6 0 251 236 427 779.6 0 427 237 636 779.6 0 636 238 565 779.6 0 565 239 480 779.6 0 480 240 418 779.6 0 418 241 224 779.6 0 224 242 265 779.6 0 265 243 238 779.6 0 238 244 418 779.6 0 418 245 577 779.6 0 577 246 899 779.6 0 899 247 820 779.6 0 820 248 649 779.6 0 649 249 381 779.6 0 381 250 154 779.6 0 154 251 126 779.6 0 126 252 79 779.6 0 79 253 225 779.6 0 225 254 383 779.6 0 383 255 400 779.6 0 400 256 439 779.6 0 439 257 640 779.6 0 640 258 1046 779.6 0 1046 259 719 779.6 0 719 260 418 779.6 0 418 261 168 779.6 0 168 262 167 779.6 0 167 263 259 779.6 0 259 264 323 779.6 0 323 265 347 779.6 0 347 266 358 779.6 0 358 267 618 779.6 0 618 268 634 779.6 0 634 269 1117 779.6 0 1117 270 486 779.6 0 486 271 347 779.6 0 347 272 238 779.6 0 238 273 293 779.6 0 293 274 337 779.6 0 337 275 380 779.6 0 380 276 400 779.6 0 400 277 207 779.6 0 207 278 182 779.6 0 182 279 308 779.6 0 308 280 471 779.6 0 471 281 468 779.6 0 468 282 550 779.6 0 550 283 240 779.6 0 240 284 267 779.6 0 267 285 338 779.6 0 338 286 399 779.6 0 399 287 646 779.6 0 646 288 583 779.6 0 583 289 480 779.6 0 480 290 286 779.6 0 286 291 194 779.6 0 194 292 294 779.6 0 294 293 393 779.6 0 393 294 487 779.6 0 487 295 781 779.6 0 781 296 587 779.6 0 587 297 335 779.6 0 335 298 262 779.6 0 262 299 231 779.6 0 231 300 177 779.6 0 177 301 462 779.6 0 462 Finished in 2.55 s (41 us/read; 1.45 M reads/minute). === Summary === Total reads processed: 61,578 Reads with adapters: 18,915 (30.7%) Reads that were too short: 1 (0.0%) Reads written (passing filters): 18,914 (30.7%) Total basepairs processed: 12,282,331 bp Total written (filtered): 5,236,775 bp (42.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 18915 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 4 16 103 0.0 3 0 102 1 17 18623 0.0 3 18319 297 6 1 18 42 0.0 3 6 36 19 10 0.0 3 0 1 9 20 114 0.0 3 106 0 0 8 22 1 0.0 3 1 30 1 0.0 3 1 39 1 0.0 3 0 0 0 1 49 1 0.0 3 0 0 0 1 51 3 0.0 3 0 0 0 3 54 1 0.0 3 0 0 0 1 55 2 0.0 3 0 0 0 2 60 4 0.0 3 0 0 0 4 73 1 0.0 3 0 0 0 1 85 3 0.0 3 0 0 0 3 190 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.87 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 18,914 Reads with adapters: 1,573 (8.3%) Reads written (passing filters): 18,914 (100.0%) Total basepairs processed: 5,236,775 bp Total written (filtered): 5,232,035 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1573 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 10.4% C: 81.0% G: 6.4% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "C" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "C" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1552 295.5 0 1552 4 21 73.9 0 21 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.