This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGGCGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 101.64 s (32 us/read; 1.88 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 19,103 (0.6%) Reads that were too short: 4,943 (0.2%) Reads written (passing filters): 14,160 (0.4%) Total basepairs processed: 961,105,341 bp Total written (filtered): 3,173,838 bp (0.3%) === Adapter 1 === Sequence: TGGCGG; Type: regular 5'; Length: 6; Trimmed: 19103 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 73 779.6 0 73 7 82 779.6 0 82 8 140 779.6 0 140 9 4388 779.6 0 4388 10 34 779.6 0 34 11 15 779.6 0 15 12 22 779.6 0 22 13 22 779.6 0 22 14 12 779.6 0 12 15 12 779.6 0 12 16 17 779.6 0 17 17 34 779.6 0 34 18 41 779.6 0 41 19 52 779.6 0 52 20 16 779.6 0 16 21 14 779.6 0 14 22 6 779.6 0 6 23 10 779.6 0 10 24 6 779.6 0 6 25 11 779.6 0 11 26 9 779.6 0 9 27 14 779.6 0 14 28 13 779.6 0 13 29 14 779.6 0 14 30 18 779.6 0 18 31 14 779.6 0 14 32 18 779.6 0 18 33 17 779.6 0 17 34 15 779.6 0 15 35 13 779.6 0 13 36 10 779.6 0 10 37 13 779.6 0 13 38 24 779.6 0 24 39 16 779.6 0 16 40 20 779.6 0 20 41 17 779.6 0 17 42 11 779.6 0 11 43 17 779.6 0 17 44 19 779.6 0 19 45 22 779.6 0 22 46 25 779.6 0 25 47 176 779.6 0 176 48 75 779.6 0 75 49 88 779.6 0 88 50 18 779.6 0 18 51 102 779.6 0 102 52 80 779.6 0 80 53 21 779.6 0 21 54 11 779.6 0 11 55 20 779.6 0 20 56 18 779.6 0 18 57 18 779.6 0 18 58 27 779.6 0 27 59 79 779.6 0 79 60 295 779.6 0 295 61 191 779.6 0 191 62 17 779.6 0 17 63 14 779.6 0 14 64 8 779.6 0 8 65 15 779.6 0 15 66 10 779.6 0 10 67 11 779.6 0 11 68 15 779.6 0 15 69 12 779.6 0 12 70 20 779.6 0 20 71 18 779.6 0 18 72 27 779.6 0 27 73 14 779.6 0 14 74 20 779.6 0 20 75 13 779.6 0 13 76 14 779.6 0 14 77 19 779.6 0 19 78 21 779.6 0 21 79 29 779.6 0 29 80 13 779.6 0 13 81 12 779.6 0 12 82 22 779.6 0 22 83 15 779.6 0 15 84 116 779.6 0 116 85 93 779.6 0 93 86 123 779.6 0 123 87 9 779.6 0 9 88 13 779.6 0 13 89 14 779.6 0 14 90 15 779.6 0 15 91 9 779.6 0 9 92 2 779.6 0 2 93 13 779.6 0 13 94 13 779.6 0 13 95 10 779.6 0 10 96 15 779.6 0 15 97 13 779.6 0 13 98 17 779.6 0 17 99 30 779.6 0 30 100 13 779.6 0 13 101 14 779.6 0 14 102 26 779.6 0 26 103 12 779.6 0 12 104 10 779.6 0 10 105 17 779.6 0 17 106 26 779.6 0 26 107 20 779.6 0 20 108 24 779.6 0 24 109 25 779.6 0 25 110 23 779.6 0 23 111 24 779.6 0 24 112 310 779.6 0 310 113 804 779.6 0 804 114 1375 779.6 0 1375 115 19 779.6 0 19 116 12 779.6 0 12 117 18 779.6 0 18 118 7 779.6 0 7 119 12 779.6 0 12 120 6 779.6 0 6 121 9 779.6 0 9 122 8 779.6 0 8 123 19 779.6 0 19 124 17 779.6 0 17 125 10 779.6 0 10 126 29 779.6 0 29 127 37 779.6 0 37 128 54 779.6 0 54 129 60 779.6 0 60 130 19 779.6 0 19 131 51 779.6 0 51 132 114 779.6 0 114 133 87 779.6 0 87 134 119 779.6 0 119 135 80 779.6 0 80 136 24 779.6 0 24 137 23 779.6 0 23 138 18 779.6 0 18 139 41 779.6 0 41 140 44 779.6 0 44 141 871 779.6 0 871 142 581 779.6 0 581 143 341 779.6 0 341 144 14 779.6 0 14 145 25 779.6 0 25 146 10 779.6 0 10 147 30 779.6 0 30 148 29 779.6 0 29 149 25 779.6 0 25 150 20 779.6 0 20 151 23 779.6 0 23 152 84 779.6 0 84 153 282 779.6 0 282 154 199 779.6 0 199 155 44 779.6 0 44 156 12 779.6 0 12 157 14 779.6 0 14 158 11 779.6 0 11 159 18 779.6 0 18 160 7 779.6 0 7 161 10 779.6 0 10 162 18 779.6 0 18 163 9 779.6 0 9 164 7 779.6 0 7 165 6 779.6 0 6 166 14 779.6 0 14 167 13 779.6 0 13 168 11 779.6 0 11 169 5 779.6 0 5 170 10 779.6 0 10 171 11 779.6 0 11 172 8 779.6 0 8 173 15 779.6 0 15 174 10 779.6 0 10 175 9 779.6 0 9 176 10 779.6 0 10 177 5 779.6 0 5 178 3 779.6 0 3 179 7 779.6 0 7 180 6 779.6 0 6 181 9 779.6 0 9 182 11 779.6 0 11 183 12 779.6 0 12 184 13 779.6 0 13 185 42 779.6 0 42 186 8 779.6 0 8 187 6 779.6 0 6 188 6 779.6 0 6 189 10 779.6 0 10 190 13 779.6 0 13 191 10 779.6 0 10 192 13 779.6 0 13 193 18 779.6 0 18 194 6 779.6 0 6 195 13 779.6 0 13 196 9 779.6 0 9 197 3 779.6 0 3 198 8 779.6 0 8 199 12 779.6 0 12 200 9 779.6 0 9 201 13 779.6 0 13 202 13 779.6 0 13 203 13 779.6 0 13 204 8 779.6 0 8 205 10 779.6 0 10 206 8 779.6 0 8 207 6 779.6 0 6 208 9 779.6 0 9 209 6 779.6 0 6 210 11 779.6 0 11 211 17 779.6 0 17 212 19 779.6 0 19 213 45 779.6 0 45 214 26 779.6 0 26 215 15 779.6 0 15 216 13 779.6 0 13 217 31 779.6 0 31 218 19 779.6 0 19 219 20 779.6 0 20 220 17 779.6 0 17 221 9 779.6 0 9 222 8 779.6 0 8 223 18 779.6 0 18 224 9 779.6 0 9 225 13 779.6 0 13 226 22 779.6 0 22 227 25 779.6 0 25 228 304 779.6 0 304 229 109 779.6 0 109 230 180 779.6 0 180 231 24 779.6 0 24 232 15 779.6 0 15 233 7 779.6 0 7 234 68 779.6 0 68 235 18 779.6 0 18 236 13 779.6 0 13 237 31 779.6 0 31 238 28 779.6 0 28 239 20 779.6 0 20 240 13 779.6 0 13 241 7 779.6 0 7 242 5 779.6 0 5 243 4 779.6 0 4 244 5 779.6 0 5 245 16 779.6 0 16 246 11 779.6 0 11 247 9 779.6 0 9 248 13 779.6 0 13 249 13 779.6 0 13 250 13 779.6 0 13 251 7 779.6 0 7 252 9 779.6 0 9 253 13 779.6 0 13 254 26 779.6 0 26 255 48 779.6 0 48 256 86 779.6 0 86 257 64 779.6 0 64 258 27 779.6 0 27 259 49 779.6 0 49 260 51 779.6 0 51 261 9 779.6 0 9 262 13 779.6 0 13 263 8 779.6 0 8 264 9 779.6 0 9 265 7 779.6 0 7 266 11 779.6 0 11 267 10 779.6 0 10 268 9 779.6 0 9 269 5 779.6 0 5 270 6 779.6 0 6 271 9 779.6 0 9 272 19 779.6 0 19 273 165 779.6 0 165 274 100 779.6 0 100 275 314 779.6 0 314 276 10 779.6 0 10 277 5 779.6 0 5 278 11 779.6 0 11 279 12 779.6 0 12 280 15 779.6 0 15 281 14 779.6 0 14 282 20 779.6 0 20 283 21 779.6 0 21 284 27 779.6 0 27 285 50 779.6 0 50 286 21 779.6 0 21 287 15 779.6 0 15 288 7 779.6 0 7 289 12 779.6 0 12 290 9 779.6 0 9 291 24 779.6 0 24 292 115 779.6 0 115 293 64 779.6 0 64 294 7 779.6 0 7 295 16 779.6 0 16 296 11 779.6 0 11 297 15 779.6 0 15 298 65 779.6 0 65 299 721 779.6 0 721 300 1222 779.6 0 1222 301 24 779.6 0 24 Finished in 0.65 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 14,160 Reads with adapters: 4,579 (32.3%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 4,575 (32.3%) Total basepairs processed: 3,173,838 bp Total written (filtered): 1,255,281 bp (39.6%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 4579 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 4 16 32 0.0 3 0 32 17 4485 0.0 3 4397 84 4 18 16 0.0 3 1 9 5 1 19 11 0.0 3 3 0 1 7 20 4 0.0 3 0 0 0 4 21 2 0.0 3 2 25 1 0.0 3 1 39 1 0.0 3 0 0 0 1 42 2 0.0 3 0 0 0 2 50 2 0.0 3 0 0 0 2 53 5 0.0 3 0 0 1 4 79 3 0.0 3 0 0 0 3 82 1 0.0 3 0 0 0 1 91 1 0.0 3 0 0 0 1 101 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 143 3 0.0 3 0 0 0 3 154 1 0.0 3 0 0 0 1 174 1 0.0 3 0 0 0 1 203 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.20 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 4,575 Reads with adapters: 364 (8.0%) Reads written (passing filters): 4,575 (100.0%) Total basepairs processed: 1,255,281 bp Total written (filtered): 1,254,188 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 364 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 62.9% C: 3.3% G: 32.7% T: 1.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 363 71.5 0 363 4 1 17.9 0 1