This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 106.12 s (33 us/read; 1.81 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 450,985 (14.1%) Reads that were too short: 9,080 (0.3%) Reads written (passing filters): 441,905 (13.8%) Total basepairs processed: 961,105,341 bp Total written (filtered): 106,269,572 bp (11.1%) === Adapter 1 === Sequence: TCCAGC; Type: regular 5'; Length: 6; Trimmed: 450985 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1574 779.6 0 1574 7 2669 779.6 0 2669 8 107921 779.6 0 107921 9 9762 779.6 0 9762 10 35 779.6 0 35 11 43 779.6 0 43 12 24 779.6 0 24 13 22 779.6 0 22 14 17 779.6 0 17 15 23 779.6 0 23 16 16 779.6 0 16 17 27 779.6 0 27 18 35 779.6 0 35 19 28 779.6 0 28 20 42 779.6 0 42 21 32 779.6 0 32 22 45 779.6 0 45 23 47 779.6 0 47 24 70 779.6 0 70 25 26 779.6 0 26 26 20 779.6 0 20 27 19 779.6 0 19 28 26 779.6 0 26 29 17 779.6 0 17 30 51 779.6 0 51 31 33 779.6 0 33 32 29 779.6 0 29 33 41 779.6 0 41 34 44 779.6 0 44 35 54 779.6 0 54 36 109 779.6 0 109 37 74 779.6 0 74 38 246 779.6 0 246 39 89 779.6 0 89 40 67 779.6 0 67 41 37 779.6 0 37 42 100 779.6 0 100 43 93 779.6 0 93 44 180 779.6 0 180 45 247 779.6 0 247 46 302 779.6 0 302 47 475 779.6 0 475 48 1181 779.6 0 1181 49 2587 779.6 0 2587 50 517 779.6 0 517 51 714 779.6 0 714 52 1390 779.6 0 1390 53 583 779.6 0 583 54 517 779.6 0 517 55 899 779.6 0 899 56 803 779.6 0 803 57 1182 779.6 0 1182 58 1198 779.6 0 1198 59 1689 779.6 0 1689 60 1962 779.6 0 1962 61 3839 779.6 0 3839 62 63787 779.6 0 63787 63 62319 779.6 0 62319 64 104570 779.6 0 104570 65 1655 779.6 0 1655 66 28 779.6 0 28 67 31 779.6 0 31 68 18 779.6 0 18 69 28 779.6 0 28 70 58 779.6 0 58 71 71 779.6 0 71 72 101 779.6 0 101 73 90 779.6 0 90 74 120 779.6 0 120 75 153 779.6 0 153 76 1998 779.6 0 1998 77 1745 779.6 0 1745 78 4598 779.6 0 4598 79 31 779.6 0 31 80 35 779.6 0 35 81 27 779.6 0 27 82 34 779.6 0 34 83 37 779.6 0 37 84 31 779.6 0 31 85 29 779.6 0 29 86 21 779.6 0 21 87 58 779.6 0 58 88 19 779.6 0 19 89 14 779.6 0 14 90 15 779.6 0 15 91 27 779.6 0 27 92 28 779.6 0 28 93 37 779.6 0 37 94 24 779.6 0 24 95 36 779.6 0 36 96 11 779.6 0 11 97 21 779.6 0 21 98 8 779.6 0 8 99 17 779.6 0 17 100 8 779.6 0 8 101 29 779.6 0 29 102 34 779.6 0 34 103 45 779.6 0 45 104 60 779.6 0 60 105 17 779.6 0 17 106 33 779.6 0 33 107 154 779.6 0 154 108 69 779.6 0 69 109 100 779.6 0 100 110 27 779.6 0 27 111 16 779.6 0 16 112 34 779.6 0 34 113 24 779.6 0 24 114 12 779.6 0 12 115 22 779.6 0 22 116 38 779.6 0 38 117 31 779.6 0 31 118 19 779.6 0 19 119 20 779.6 0 20 120 39 779.6 0 39 121 25 779.6 0 25 122 34 779.6 0 34 123 38 779.6 0 38 124 35 779.6 0 35 125 32 779.6 0 32 126 43 779.6 0 43 127 46 779.6 0 46 128 62 779.6 0 62 129 49 779.6 0 49 130 29 779.6 0 29 131 29 779.6 0 29 132 43 779.6 0 43 133 34 779.6 0 34 134 34 779.6 0 34 135 37 779.6 0 37 136 28 779.6 0 28 137 79 779.6 0 79 138 52 779.6 0 52 139 46 779.6 0 46 140 67 779.6 0 67 141 99 779.6 0 99 142 246 779.6 0 246 143 261 779.6 0 261 144 3965 779.6 0 3965 145 1348 779.6 0 1348 146 3887 779.6 0 3887 147 151 779.6 0 151 148 47 779.6 0 47 149 132 779.6 0 132 150 242 779.6 0 242 151 254 779.6 0 254 152 89 779.6 0 89 153 43 779.6 0 43 154 50 779.6 0 50 155 61 779.6 0 61 156 230 779.6 0 230 157 236 779.6 0 236 158 359 779.6 0 359 159 156 779.6 0 156 160 216 779.6 0 216 161 243 779.6 0 243 162 456 779.6 0 456 163 577 779.6 0 577 164 6435 779.6 0 6435 165 4812 779.6 0 4812 166 16810 779.6 0 16810 167 171 779.6 0 171 168 175 779.6 0 175 169 903 779.6 0 903 170 1259 779.6 0 1259 171 953 779.6 0 953 172 1361 779.6 0 1361 173 616 779.6 0 616 174 367 779.6 0 367 175 43 779.6 0 43 176 51 779.6 0 51 177 42 779.6 0 42 178 49 779.6 0 49 179 142 779.6 0 142 180 122 779.6 0 122 181 97 779.6 0 97 182 164 779.6 0 164 183 141 779.6 0 141 184 253 779.6 0 253 185 186 779.6 0 186 186 265 779.6 0 265 187 172 779.6 0 172 188 314 779.6 0 314 189 967 779.6 0 967 190 2290 779.6 0 2290 191 1165 779.6 0 1165 192 454 779.6 0 454 193 30 779.6 0 30 194 24 779.6 0 24 195 27 779.6 0 27 196 42 779.6 0 42 197 24 779.6 0 24 198 52 779.6 0 52 199 29 779.6 0 29 200 33 779.6 0 33 201 42 779.6 0 42 202 22 779.6 0 22 203 28 779.6 0 28 204 29 779.6 0 29 205 35 779.6 0 35 206 52 779.6 0 52 207 178 779.6 0 178 208 73 779.6 0 73 209 42 779.6 0 42 210 30 779.6 0 30 211 26 779.6 0 26 212 66 779.6 0 66 213 43 779.6 0 43 214 59 779.6 0 59 215 53 779.6 0 53 216 91 779.6 0 91 217 178 779.6 0 178 218 128 779.6 0 128 219 96 779.6 0 96 220 32 779.6 0 32 221 71 779.6 0 71 222 20 779.6 0 20 223 28 779.6 0 28 224 62 779.6 0 62 225 108 779.6 0 108 226 146 779.6 0 146 227 154 779.6 0 154 228 169 779.6 0 169 229 118 779.6 0 118 230 162 779.6 0 162 231 207 779.6 0 207 232 72 779.6 0 72 233 43 779.6 0 43 234 14 779.6 0 14 235 25 779.6 0 25 236 24 779.6 0 24 237 28 779.6 0 28 238 21 779.6 0 21 239 26 779.6 0 26 240 50 779.6 0 50 241 38 779.6 0 38 242 15 779.6 0 15 243 21 779.6 0 21 244 58 779.6 0 58 245 146 779.6 0 146 246 101 779.6 0 101 247 59 779.6 0 59 248 64 779.6 0 64 249 39 779.6 0 39 250 37 779.6 0 37 251 40 779.6 0 40 252 31 779.6 0 31 253 39 779.6 0 39 254 75 779.6 0 75 255 106 779.6 0 106 256 99 779.6 0 99 257 67 779.6 0 67 258 33 779.6 0 33 259 82 779.6 0 82 260 215 779.6 0 215 261 253 779.6 0 253 262 492 779.6 0 492 263 272 779.6 0 272 264 50 779.6 0 50 265 39 779.6 0 39 266 74 779.6 0 74 267 102 779.6 0 102 268 104 779.6 0 104 269 78 779.6 0 78 270 53 779.6 0 53 271 32 779.6 0 32 272 58 779.6 0 58 273 33 779.6 0 33 274 37 779.6 0 37 275 45 779.6 0 45 276 49 779.6 0 49 277 61 779.6 0 61 278 59 779.6 0 59 279 100 779.6 0 100 280 173 779.6 0 173 281 81 779.6 0 81 282 130 779.6 0 130 283 41 779.6 0 41 284 60 779.6 0 60 285 58 779.6 0 58 286 105 779.6 0 105 287 488 779.6 0 488 288 552 779.6 0 552 289 312 779.6 0 312 290 92 779.6 0 92 291 50 779.6 0 50 292 53 779.6 0 53 293 62 779.6 0 62 294 139 779.6 0 139 295 66 779.6 0 66 296 138 779.6 0 138 297 142 779.6 0 142 298 89 779.6 0 89 299 46 779.6 0 46 300 22 779.6 0 22 301 16 779.6 0 16 Finished in 24.78 s (56 us/read; 1.07 M reads/minute). === Summary === Total reads processed: 441,905 Reads with adapters: 15,845 (3.6%) Reads that were too short: 194 (0.0%) Reads written (passing filters): 15,651 (3.5%) Total basepairs processed: 106,269,572 bp Total written (filtered): 3,681,465 bp (3.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 15845 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 9 0.0 3 0 0 7 1 1 19 125 0.0 3 0 119 4 1 1 20 10033 0.0 4 9699 275 37 15 7 21 26 0.0 4 5 17 1 0 3 23 4 0.0 4 0 0 0 4 24 1 0.0 4 1 25 1 0.0 4 0 0 0 0 1 26 2 0.0 4 0 0 0 0 2 29 1 0.0 4 0 0 0 0 1 34 22 0.0 4 0 0 0 0 22 35 1 0.0 4 1 36 1 0.0 4 0 0 0 0 1 37 2 0.0 4 0 0 0 0 2 51 3 0.0 4 0 0 0 0 3 52 1 0.0 4 0 0 0 0 1 53 1 0.0 4 0 0 0 0 1 61 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 69 1 0.0 4 0 0 0 0 1 70 10 0.0 4 0 0 0 0 10 71 9 0.0 4 0 0 0 0 9 72 5364 0.0 4 0 0 0 0 5364 74 1 0.0 4 0 0 0 0 1 75 3 0.0 4 0 0 0 0 3 76 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 91 2 0.0 4 0 0 0 0 2 101 2 0.0 4 0 0 0 0 2 104 1 0.0 4 0 0 0 0 1 105 1 0.0 4 0 0 0 0 1 111 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 0 0 0 1 130 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 152 3 0.0 4 0 0 0 0 3 153 36 0.0 4 0 0 0 0 36 154 2 0.0 4 0 0 0 0 2 158 1 0.0 4 0 0 0 0 1 161 1 0.0 4 0 0 0 0 1 166 1 0.0 4 0 0 0 0 1 167 4 0.0 4 0 0 1 0 3 168 1 0.0 4 0 0 0 0 1 169 1 0.0 4 0 0 0 0 1 170 3 0.0 4 0 0 0 0 3 173 29 0.0 4 0 0 0 0 29 174 3 0.0 4 0 0 0 0 3 175 1 0.0 4 0 0 0 0 1 177 1 0.0 4 0 0 0 0 1 178 1 0.0 4 0 0 0 0 1 179 4 0.0 4 0 0 0 0 4 180 2 0.0 4 0 0 0 0 2 183 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 199 1 0.0 4 0 0 0 0 1 201 1 0.0 4 0 0 0 0 1 202 2 0.0 4 0 0 0 0 2 203 2 0.0 4 0 0 0 0 2 208 1 0.0 4 0 0 0 0 1 214 2 0.0 4 0 0 0 1 1 224 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 227 24 0.0 4 0 0 0 1 23 228 6 0.0 4 0 0 0 0 6 229 2 0.0 4 0 0 0 0 2 230 3 0.0 4 0 0 0 0 3 233 7 0.0 4 0 0 0 0 7 234 7 0.0 4 0 0 0 0 7 235 7 0.0 4 0 0 0 0 7 236 12 0.0 4 0 0 0 0 12 237 5 0.0 4 0 0 0 0 5 238 1 0.0 4 0 0 0 0 1 239 1 0.0 4 0 0 0 0 1 242 1 0.0 4 0 0 0 0 1 247 1 0.0 4 0 0 0 0 1 255 1 0.0 4 0 0 0 0 1 256 2 0.0 4 0 0 0 0 2 257 1 0.0 4 0 0 0 0 1 258 4 0.0 4 0 0 0 1 3 259 2 0.0 4 0 0 0 0 2 260 8 0.0 4 0 0 0 0 8 265 2 0.0 4 0 0 0 0 2 282 3 0.0 4 0 0 0 0 3 286 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.61 s (39 us/read; 1.54 M reads/minute). === Summary === Total reads processed: 15,651 Reads with adapters: 822 (5.3%) Reads written (passing filters): 15,651 (100.0%) Total basepairs processed: 3,681,465 bp Total written (filtered): 3,655,530 bp (99.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 822 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 18.9% C: 2.3% G: 77.3% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5 244.5 0 5 4 29 61.1 0 29 5 4 15.3 0 4 6 1 3.8 0 1 8 1 0.2 0 1 9 142 0.1 0 5 137 10 199 0.0 1 5 194 11 343 0.0 1 1 342 12 1 0.0 1 0 1 150 1 0.0 1 0 1 192 1 0.0 1 1 193 86 0.0 1 80 6 194 5 0.0 1 4 1 195 1 0.0 1 1 196 1 0.0 1 1 208 2 0.0 1 1 1