This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATCCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 108.30 s (34 us/read; 1.77 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 1,475,735 (46.2%) Reads that were too short: 61,974 (1.9%) Reads written (passing filters): 1,413,761 (44.3%) Total basepairs processed: 961,105,341 bp Total written (filtered): 380,265,661 bp (39.6%) === Adapter 1 === Sequence: AATCCT; Type: regular 5'; Length: 6; Trimmed: 1475735 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 207 779.6 0 207 7 157 779.6 0 157 8 363 779.6 0 363 9 9525 779.6 0 9525 10 419 779.6 0 419 11 434 779.6 0 434 12 1300 779.6 0 1300 13 3761 779.6 0 3761 14 583 779.6 0 583 15 1061 779.6 0 1061 16 716 779.6 0 716 17 909 779.6 0 909 18 2091 779.6 0 2091 19 1386 779.6 0 1386 20 1512 779.6 0 1512 21 760 779.6 0 760 22 841 779.6 0 841 23 1490 779.6 0 1490 24 2015 779.6 0 2015 25 4304 779.6 0 4304 26 5529 779.6 0 5529 27 7794 779.6 0 7794 28 16332 779.6 0 16332 29 24475 779.6 0 24475 30 360308 779.6 0 360308 31 472826 779.6 0 472826 32 468759 779.6 0 468759 33 2322 779.6 0 2322 34 210 779.6 0 210 35 165 779.6 0 165 36 316 779.6 0 316 37 202 779.6 0 202 38 19 779.6 0 19 39 12 779.6 0 12 40 11 779.6 0 11 41 10 779.6 0 10 42 14 779.6 0 14 43 37 779.6 0 37 44 10 779.6 0 10 45 12 779.6 0 12 46 16 779.6 0 16 47 14 779.6 0 14 48 12 779.6 0 12 49 10 779.6 0 10 50 12 779.6 0 12 51 7 779.6 0 7 52 13 779.6 0 13 53 18 779.6 0 18 54 291 779.6 0 291 55 1091 779.6 0 1091 56 118 779.6 0 118 57 5 779.6 0 5 58 4 779.6 0 4 59 8 779.6 0 8 60 5 779.6 0 5 61 6 779.6 0 6 62 6 779.6 0 6 63 6 779.6 0 6 64 5 779.6 0 5 65 5 779.6 0 5 66 4 779.6 0 4 67 3 779.6 0 3 68 2 779.6 0 2 69 7 779.6 0 7 70 4 779.6 0 4 71 5 779.6 0 5 72 5 779.6 0 5 73 6 779.6 0 6 74 10 779.6 0 10 75 5 779.6 0 5 76 11 779.6 0 11 77 30 779.6 0 30 78 36 779.6 0 36 79 44 779.6 0 44 80 43 779.6 0 43 81 73 779.6 0 73 82 42 779.6 0 42 83 43 779.6 0 43 84 57 779.6 0 57 85 62 779.6 0 62 86 36 779.6 0 36 87 193 779.6 0 193 88 46 779.6 0 46 89 89 779.6 0 89 90 77 779.6 0 77 91 15 779.6 0 15 92 29 779.6 0 29 93 176 779.6 0 176 94 128 779.6 0 128 95 36 779.6 0 36 96 22 779.6 0 22 97 32 779.6 0 32 98 40 779.6 0 40 99 80 779.6 0 80 100 298 779.6 0 298 101 141 779.6 0 141 102 202 779.6 0 202 103 209 779.6 0 209 104 486 779.6 0 486 105 365 779.6 0 365 106 820 779.6 0 820 107 63 779.6 0 63 108 14 779.6 0 14 109 42 779.6 0 42 110 122 779.6 0 122 111 116 779.6 0 116 112 59 779.6 0 59 113 49 779.6 0 49 114 50 779.6 0 50 115 43 779.6 0 43 116 48 779.6 0 48 117 46 779.6 0 46 118 352 779.6 0 352 119 215 779.6 0 215 120 179 779.6 0 179 121 58 779.6 0 58 122 73 779.6 0 73 123 87 779.6 0 87 124 378 779.6 0 378 125 565 779.6 0 565 126 4522 779.6 0 4522 127 59 779.6 0 59 128 23 779.6 0 23 129 31 779.6 0 31 130 59 779.6 0 59 131 135 779.6 0 135 132 105 779.6 0 105 133 68 779.6 0 68 134 56 779.6 0 56 135 57 779.6 0 57 136 49 779.6 0 49 137 123 779.6 0 123 138 228 779.6 0 228 139 119 779.6 0 119 140 55 779.6 0 55 141 66 779.6 0 66 142 94 779.6 0 94 143 77 779.6 0 77 144 153 779.6 0 153 145 70 779.6 0 70 146 64 779.6 0 64 147 58 779.6 0 58 148 37 779.6 0 37 149 105 779.6 0 105 150 77 779.6 0 77 151 59 779.6 0 59 152 43 779.6 0 43 153 69 779.6 0 69 154 172 779.6 0 172 155 68 779.6 0 68 156 63 779.6 0 63 157 157 779.6 0 157 158 146 779.6 0 146 159 549 779.6 0 549 160 530 779.6 0 530 161 302 779.6 0 302 162 68 779.6 0 68 163 108 779.6 0 108 164 217 779.6 0 217 165 211 779.6 0 211 166 104 779.6 0 104 167 99 779.6 0 99 168 103 779.6 0 103 169 58 779.6 0 58 170 45 779.6 0 45 171 76 779.6 0 76 172 57 779.6 0 57 173 59 779.6 0 59 174 57 779.6 0 57 175 23 779.6 0 23 176 20 779.6 0 20 177 68 779.6 0 68 178 91 779.6 0 91 179 177 779.6 0 177 180 200 779.6 0 200 181 99 779.6 0 99 182 107 779.6 0 107 183 117 779.6 0 117 184 168 779.6 0 168 185 93 779.6 0 93 186 45 779.6 0 45 187 39 779.6 0 39 188 85 779.6 0 85 189 92 779.6 0 92 190 195 779.6 0 195 191 106 779.6 0 106 192 66 779.6 0 66 193 29 779.6 0 29 194 33 779.6 0 33 195 50 779.6 0 50 196 81 779.6 0 81 197 115 779.6 0 115 198 83 779.6 0 83 199 104 779.6 0 104 200 60 779.6 0 60 201 85 779.6 0 85 202 104 779.6 0 104 203 242 779.6 0 242 204 321 779.6 0 321 205 192 779.6 0 192 206 199 779.6 0 199 207 92 779.6 0 92 208 23 779.6 0 23 209 44 779.6 0 44 210 77 779.6 0 77 211 81 779.6 0 81 212 64 779.6 0 64 213 54 779.6 0 54 214 81 779.6 0 81 215 147 779.6 0 147 216 115 779.6 0 115 217 119 779.6 0 119 218 135 779.6 0 135 219 60 779.6 0 60 220 83 779.6 0 83 221 28 779.6 0 28 222 66 779.6 0 66 223 250 779.6 0 250 224 251 779.6 0 251 225 135 779.6 0 135 226 168 779.6 0 168 227 131 779.6 0 131 228 146 779.6 0 146 229 62 779.6 0 62 230 96 779.6 0 96 231 80 779.6 0 80 232 81 779.6 0 81 233 107 779.6 0 107 234 169 779.6 0 169 235 94 779.6 0 94 236 56 779.6 0 56 237 43 779.6 0 43 238 31 779.6 0 31 239 66 779.6 0 66 240 132 779.6 0 132 241 199 779.6 0 199 242 135 779.6 0 135 243 208 779.6 0 208 244 259 779.6 0 259 245 192 779.6 0 192 246 148 779.6 0 148 247 233 779.6 0 233 248 104 779.6 0 104 249 123 779.6 0 123 250 240 779.6 0 240 251 244 779.6 0 244 252 441 779.6 0 441 253 657 779.6 0 657 254 6354 779.6 0 6354 255 4679 779.6 0 4679 256 9007 779.6 0 9007 257 846 779.6 0 846 258 448 779.6 0 448 259 428 779.6 0 428 260 3806 779.6 0 3806 261 5679 779.6 0 5679 262 7718 779.6 0 7718 263 341 779.6 0 341 264 239 779.6 0 239 265 152 779.6 0 152 266 71 779.6 0 71 267 51 779.6 0 51 268 63 779.6 0 63 269 53 779.6 0 53 270 91 779.6 0 91 271 94 779.6 0 94 272 257 779.6 0 257 273 447 779.6 0 447 274 714 779.6 0 714 275 1294 779.6 0 1294 276 583 779.6 0 583 277 2144 779.6 0 2144 278 1942 779.6 0 1942 279 3867 779.6 0 3867 280 1522 779.6 0 1522 281 82 779.6 0 82 282 187 779.6 0 187 283 142 779.6 0 142 284 91 779.6 0 91 285 68 779.6 0 68 286 36 779.6 0 36 287 15 779.6 0 15 288 13 779.6 0 13 289 20 779.6 0 20 290 47 779.6 0 47 291 156 779.6 0 156 292 114 779.6 0 114 293 63 779.6 0 63 294 33 779.6 0 33 295 15 779.6 0 15 296 13 779.6 0 13 297 27 779.6 0 27 298 40 779.6 0 40 299 67 779.6 0 67 300 161 779.6 0 161 301 86 779.6 0 86 Finished in 72.94 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 1,413,761 Reads with adapters: 10,797 (0.8%) Reads that were too short: 111 (0.0%) Reads written (passing filters): 10,686 (0.8%) Total basepairs processed: 380,265,661 bp Total written (filtered): 2,804,335 bp (0.7%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 10797 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 73 0.0 3 0 68 5 17 9686 0.0 3 9477 202 7 18 26 0.0 3 0 20 1 5 19 6 0.0 3 1 0 5 20 105 0.0 3 91 1 0 13 25 2 0.0 3 0 0 1 1 32 1 0.0 3 0 0 0 1 44 1 0.0 3 0 0 0 1 67 2 0.0 3 0 0 1 1 68 5 0.0 3 0 0 0 5 70 4 0.0 3 0 0 0 4 71 2 0.0 3 0 0 0 2 73 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 87 1 0.0 3 0 0 0 1 88 1 0.0 3 0 0 0 1 92 1 0.0 3 1 96 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 112 1 0.0 3 1 130 1 0.0 3 0 0 0 1 136 2 0.0 3 0 0 0 2 137 6 0.0 3 0 0 0 6 139 1 0.0 3 0 0 0 1 140 4 0.0 3 0 0 0 4 141 26 0.0 3 0 0 0 26 156 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 159 1 0.0 3 0 0 0 1 168 13 0.0 3 0 0 0 13 169 710 0.0 3 0 0 0 710 170 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 3 0.0 3 0 0 0 3 179 1 0.0 3 0 0 0 1 180 4 0.0 3 0 0 0 4 181 2 0.0 3 0 0 0 2 182 1 0.0 3 0 0 0 1 183 3 0.0 3 0 0 0 3 184 1 0.0 3 0 0 0 1 191 1 0.0 3 0 0 0 1 194 1 0.0 3 0 0 0 1 198 4 0.0 3 0 0 0 4 199 1 0.0 3 0 0 0 1 221 1 0.0 3 0 0 0 1 233 1 0.0 3 0 0 0 1 234 1 0.0 3 0 0 0 1 247 1 0.0 3 0 0 0 1 256 3 0.0 3 0 0 0 3 257 6 0.0 3 0 0 1 5 258 14 0.0 3 0 0 0 14 259 28 0.0 3 0 0 0 28 260 12 0.0 3 0 0 0 12 261 1 0.0 3 0 0 0 1 264 1 0.0 3 0 0 0 1 265 10 0.0 3 0 0 0 10 266 3 0.0 3 0 0 0 3 267 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.47 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 10,686 Reads with adapters: 514 (4.8%) Reads written (passing filters): 10,686 (100.0%) Total basepairs processed: 2,804,335 bp Total written (filtered): 2,802,788 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 514 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 52.9% C: 8.8% G: 37.0% T: 1.4% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 509 167.0 0 509 4 5 41.7 0 5