This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTAACA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 104.78 s (33 us/read; 1.83 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 111,230 (3.5%) Reads that were too short: 32,971 (1.0%) Reads written (passing filters): 78,259 (2.5%) Total basepairs processed: 961,105,341 bp Total written (filtered): 17,040,144 bp (1.8%) === Adapter 1 === Sequence: GTAACA; Type: regular 5'; Length: 6; Trimmed: 111230 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 9157 779.6 0 9157 7 274 779.6 0 274 8 14806 779.6 0 14806 9 31 779.6 0 31 10 8 779.6 0 8 11 6 779.6 0 6 12 10 779.6 0 10 13 8 779.6 0 8 14 11 779.6 0 11 15 9 779.6 0 9 16 11 779.6 0 11 17 12 779.6 0 12 18 14 779.6 0 14 19 7 779.6 0 7 20 5 779.6 0 5 21 5 779.6 0 5 22 6 779.6 0 6 23 5 779.6 0 5 24 11 779.6 0 11 25 13 779.6 0 13 26 5 779.6 0 5 27 4 779.6 0 4 28 11 779.6 0 11 29 7 779.6 0 7 30 15 779.6 0 15 31 12 779.6 0 12 32 15 779.6 0 15 33 100 779.6 0 100 34 20 779.6 0 20 35 7 779.6 0 7 36 7 779.6 0 7 37 14 779.6 0 14 38 13 779.6 0 13 39 12 779.6 0 12 40 7 779.6 0 7 41 12 779.6 0 12 42 48 779.6 0 48 43 12 779.6 0 12 44 9 779.6 0 9 45 11 779.6 0 11 46 6 779.6 0 6 47 4 779.6 0 4 48 8 779.6 0 8 49 12 779.6 0 12 50 8 779.6 0 8 51 41 779.6 0 41 52 79 779.6 0 79 53 25 779.6 0 25 54 42 779.6 0 42 55 18 779.6 0 18 56 18 779.6 0 18 57 36 779.6 0 36 58 16 779.6 0 16 59 25 779.6 0 25 60 19 779.6 0 19 61 11 779.6 0 11 62 31 779.6 0 31 63 37 779.6 0 37 64 74 779.6 0 74 65 97 779.6 0 97 66 137 779.6 0 137 67 314 779.6 0 314 68 579 779.6 0 579 69 9418 779.6 0 9418 70 3970 779.6 0 3970 71 6711 779.6 0 6711 72 37 779.6 0 37 73 6 779.6 0 6 74 12 779.6 0 12 75 7 779.6 0 7 76 9 779.6 0 9 77 8 779.6 0 8 78 11 779.6 0 11 79 13 779.6 0 13 80 101 779.6 0 101 81 313 779.6 0 313 82 335 779.6 0 335 83 73 779.6 0 73 84 199 779.6 0 199 85 161 779.6 0 161 86 83 779.6 0 83 87 40 779.6 0 40 88 134 779.6 0 134 89 17 779.6 0 17 90 21 779.6 0 21 91 50 779.6 0 50 92 107 779.6 0 107 93 21 779.6 0 21 94 24 779.6 0 24 95 64 779.6 0 64 96 150 779.6 0 150 97 137 779.6 0 137 98 192 779.6 0 192 99 55 779.6 0 55 100 73 779.6 0 73 101 392 779.6 0 392 102 1450 779.6 0 1450 103 229 779.6 0 229 104 284 779.6 0 284 105 110 779.6 0 110 106 35 779.6 0 35 107 114 779.6 0 114 108 328 779.6 0 328 109 84 779.6 0 84 110 20 779.6 0 20 111 49 779.6 0 49 112 103 779.6 0 103 113 67 779.6 0 67 114 127 779.6 0 127 115 59 779.6 0 59 116 56 779.6 0 56 117 88 779.6 0 88 118 115 779.6 0 115 119 94 779.6 0 94 120 204 779.6 0 204 121 169 779.6 0 169 122 154 779.6 0 154 123 225 779.6 0 225 124 200 779.6 0 200 125 115 779.6 0 115 126 150 779.6 0 150 127 146 779.6 0 146 128 131 779.6 0 131 129 187 779.6 0 187 130 128 779.6 0 128 131 164 779.6 0 164 132 297 779.6 0 297 133 620 779.6 0 620 134 529 779.6 0 529 135 161 779.6 0 161 136 279 779.6 0 279 137 474 779.6 0 474 138 611 779.6 0 611 139 310 779.6 0 310 140 291 779.6 0 291 141 147 779.6 0 147 142 192 779.6 0 192 143 245 779.6 0 245 144 125 779.6 0 125 145 99 779.6 0 99 146 103 779.6 0 103 147 259 779.6 0 259 148 460 779.6 0 460 149 289 779.6 0 289 150 279 779.6 0 279 151 133 779.6 0 133 152 227 779.6 0 227 153 144 779.6 0 144 154 166 779.6 0 166 155 124 779.6 0 124 156 269 779.6 0 269 157 255 779.6 0 255 158 539 779.6 0 539 159 371 779.6 0 371 160 331 779.6 0 331 161 260 779.6 0 260 162 319 779.6 0 319 163 205 779.6 0 205 164 168 779.6 0 168 165 143 779.6 0 143 166 185 779.6 0 185 167 208 779.6 0 208 168 402 779.6 0 402 169 338 779.6 0 338 170 206 779.6 0 206 171 276 779.6 0 276 172 322 779.6 0 322 173 141 779.6 0 141 174 130 779.6 0 130 175 404 779.6 0 404 176 1720 779.6 0 1720 177 1436 779.6 0 1436 178 1417 779.6 0 1417 179 280 779.6 0 280 180 299 779.6 0 299 181 191 779.6 0 191 182 266 779.6 0 266 183 319 779.6 0 319 184 327 779.6 0 327 185 265 779.6 0 265 186 315 779.6 0 315 187 378 779.6 0 378 188 845 779.6 0 845 189 941 779.6 0 941 190 124 779.6 0 124 191 90 779.6 0 90 192 91 779.6 0 91 193 239 779.6 0 239 194 224 779.6 0 224 195 289 779.6 0 289 196 407 779.6 0 407 197 138 779.6 0 138 198 118 779.6 0 118 199 171 779.6 0 171 200 296 779.6 0 296 201 321 779.6 0 321 202 233 779.6 0 233 203 321 779.6 0 321 204 244 779.6 0 244 205 240 779.6 0 240 206 230 779.6 0 230 207 230 779.6 0 230 208 308 779.6 0 308 209 207 779.6 0 207 210 196 779.6 0 196 211 169 779.6 0 169 212 227 779.6 0 227 213 345 779.6 0 345 214 294 779.6 0 294 215 375 779.6 0 375 216 276 779.6 0 276 217 281 779.6 0 281 218 288 779.6 0 288 219 413 779.6 0 413 220 234 779.6 0 234 221 354 779.6 0 354 222 368 779.6 0 368 223 187 779.6 0 187 224 257 779.6 0 257 225 320 779.6 0 320 226 292 779.6 0 292 227 277 779.6 0 277 228 247 779.6 0 247 229 478 779.6 0 478 230 312 779.6 0 312 231 239 779.6 0 239 232 232 779.6 0 232 233 301 779.6 0 301 234 96 779.6 0 96 235 97 779.6 0 97 236 231 779.6 0 231 237 303 779.6 0 303 238 358 779.6 0 358 239 357 779.6 0 357 240 325 779.6 0 325 241 203 779.6 0 203 242 188 779.6 0 188 243 202 779.6 0 202 244 328 779.6 0 328 245 320 779.6 0 320 246 343 779.6 0 343 247 230 779.6 0 230 248 308 779.6 0 308 249 310 779.6 0 310 250 213 779.6 0 213 251 138 779.6 0 138 252 121 779.6 0 121 253 276 779.6 0 276 254 297 779.6 0 297 255 320 779.6 0 320 256 353 779.6 0 353 257 260 779.6 0 260 258 320 779.6 0 320 259 404 779.6 0 404 260 251 779.6 0 251 261 214 779.6 0 214 262 146 779.6 0 146 263 205 779.6 0 205 264 274 779.6 0 274 265 328 779.6 0 328 266 2023 779.6 0 2023 267 2049 779.6 0 2049 268 373 779.6 0 373 269 797 779.6 0 797 270 645 779.6 0 645 271 507 779.6 0 507 272 198 779.6 0 198 273 188 779.6 0 188 274 285 779.6 0 285 275 367 779.6 0 367 276 578 779.6 0 578 277 526 779.6 0 526 278 346 779.6 0 346 279 194 779.6 0 194 280 189 779.6 0 189 281 473 779.6 0 473 282 443 779.6 0 443 283 318 779.6 0 318 284 272 779.6 0 272 285 258 779.6 0 258 286 282 779.6 0 282 287 333 779.6 0 333 288 420 779.6 0 420 289 399 779.6 0 399 290 286 779.6 0 286 291 189 779.6 0 189 292 183 779.6 0 183 293 190 779.6 0 190 294 386 779.6 0 386 295 460 779.6 0 460 296 328 779.6 0 328 297 140 779.6 0 140 298 224 779.6 0 224 299 206 779.6 0 206 300 195 779.6 0 195 301 427 779.6 0 427 Finished in 3.51 s (45 us/read; 1.34 M reads/minute). === Summary === Total reads processed: 78,259 Reads with adapters: 15,166 (19.4%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 15,154 (19.4%) Total basepairs processed: 17,040,144 bp Total written (filtered): 4,174,199 bp (24.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 15166 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 5 0.0 3 0 0 5 16 105 0.0 3 0 100 4 1 17 11045 0.0 3 10823 216 6 18 3918 0.0 3 3797 120 1 19 34 0.0 3 3 6 21 4 20 14 0.0 3 1 0 2 11 21 5 0.0 3 0 0 0 5 34 3 0.0 3 0 0 0 3 37 2 0.0 3 0 0 0 2 48 2 0.0 3 0 0 0 2 55 7 0.0 3 0 0 0 7 58 4 0.0 3 0 0 0 4 61 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 91 5 0.0 3 0 0 0 5 94 2 0.0 3 0 0 0 2 104 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 119 1 0.0 3 0 0 0 1 139 1 0.0 3 0 0 0 1 148 1 0.0 3 0 0 0 1 157 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 220 1 0.0 3 0 0 0 1 283 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.64 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 15,154 Reads with adapters: 530 (3.5%) Reads written (passing filters): 15,154 (100.0%) Total basepairs processed: 4,174,199 bp Total written (filtered): 4,172,600 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 530 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 35.5% C: 19.2% G: 43.2% T: 2.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 521 236.8 0 521 4 9 59.2 0 9