This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTCGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 99.98 s (31 us/read; 1.92 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 281,782 (8.8%) Reads that were too short: 3,587 (0.1%) Reads written (passing filters): 278,195 (8.7%) Total basepairs processed: 961,105,341 bp Total written (filtered): 80,493,098 bp (8.4%) === Adapter 1 === Sequence: GGTCGA; Type: regular 5'; Length: 6; Trimmed: 281782 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 19059 779.6 0 19059 7 3519 779.6 0 3519 8 40281 779.6 0 40281 9 206837 779.6 0 206837 10 412 779.6 0 412 11 40 779.6 0 40 12 15 779.6 0 15 13 11 779.6 0 11 14 17 779.6 0 17 15 8 779.6 0 8 16 11 779.6 0 11 17 8 779.6 0 8 18 7 779.6 0 7 19 16 779.6 0 16 20 12 779.6 0 12 21 14 779.6 0 14 22 10 779.6 0 10 23 7 779.6 0 7 24 9 779.6 0 9 25 12 779.6 0 12 26 15 779.6 0 15 27 3 779.6 0 3 28 16 779.6 0 16 29 14 779.6 0 14 30 17 779.6 0 17 31 10 779.6 0 10 32 8 779.6 0 8 33 5 779.6 0 5 34 37 779.6 0 37 35 79 779.6 0 79 36 11 779.6 0 11 37 13 779.6 0 13 38 51 779.6 0 51 39 22 779.6 0 22 40 22 779.6 0 22 41 19 779.6 0 19 42 12 779.6 0 12 43 19 779.6 0 19 44 52 779.6 0 52 45 7 779.6 0 7 46 19 779.6 0 19 47 260 779.6 0 260 48 196 779.6 0 196 49 448 779.6 0 448 50 625 779.6 0 625 51 10 779.6 0 10 52 6 779.6 0 6 53 10 779.6 0 10 54 7 779.6 0 7 55 6 779.6 0 6 56 2 779.6 0 2 57 6 779.6 0 6 58 10 779.6 0 10 59 9 779.6 0 9 60 8 779.6 0 8 61 10 779.6 0 10 62 18 779.6 0 18 63 107 779.6 0 107 64 28 779.6 0 28 65 3 779.6 0 3 66 8 779.6 0 8 67 7 779.6 0 7 68 17 779.6 0 17 69 3 779.6 0 3 70 9 779.6 0 9 71 9 779.6 0 9 72 9 779.6 0 9 73 4 779.6 0 4 74 10 779.6 0 10 75 9 779.6 0 9 76 4 779.6 0 4 77 11 779.6 0 11 78 16 779.6 0 16 79 7 779.6 0 7 80 14 779.6 0 14 81 27 779.6 0 27 82 30 779.6 0 30 83 581 779.6 0 581 84 26 779.6 0 26 85 45 779.6 0 45 86 14 779.6 0 14 87 5 779.6 0 5 88 12 779.6 0 12 89 28 779.6 0 28 90 11 779.6 0 11 91 13 779.6 0 13 92 9 779.6 0 9 93 6 779.6 0 6 94 9 779.6 0 9 95 25 779.6 0 25 96 151 779.6 0 151 97 13 779.6 0 13 98 14 779.6 0 14 99 5 779.6 0 5 100 5 779.6 0 5 101 3 779.6 0 3 102 88 779.6 0 88 103 5 779.6 0 5 104 3 779.6 0 3 105 35 779.6 0 35 106 31 779.6 0 31 107 8 779.6 0 8 108 3 779.6 0 3 109 8 779.6 0 8 110 4 779.6 0 4 111 11 779.6 0 11 112 9 779.6 0 9 113 11 779.6 0 11 114 13 779.6 0 13 115 7 779.6 0 7 116 4 779.6 0 4 117 5 779.6 0 5 118 41 779.6 0 41 119 47 779.6 0 47 120 43 779.6 0 43 121 7 779.6 0 7 122 11 779.6 0 11 123 4 779.6 0 4 124 6 779.6 0 6 125 4 779.6 0 4 126 10 779.6 0 10 127 16 779.6 0 16 128 11 779.6 0 11 129 10 779.6 0 10 130 11 779.6 0 11 131 10 779.6 0 10 132 17 779.6 0 17 133 27 779.6 0 27 134 13 779.6 0 13 135 11 779.6 0 11 136 22 779.6 0 22 137 11 779.6 0 11 138 28 779.6 0 28 139 10 779.6 0 10 140 10 779.6 0 10 141 16 779.6 0 16 142 15 779.6 0 15 143 14 779.6 0 14 144 28 779.6 0 28 145 46 779.6 0 46 146 107 779.6 0 107 147 641 779.6 0 641 148 1396 779.6 0 1396 149 25 779.6 0 25 150 8 779.6 0 8 151 14 779.6 0 14 152 7 779.6 0 7 153 86 779.6 0 86 154 4 779.6 0 4 155 16 779.6 0 16 156 3 779.6 0 3 157 3 779.6 0 3 158 9 779.6 0 9 159 9 779.6 0 9 160 6 779.6 0 6 161 8 779.6 0 8 162 8 779.6 0 8 163 3 779.6 0 3 164 7 779.6 0 7 165 5 779.6 0 5 166 6 779.6 0 6 167 8 779.6 0 8 168 13 779.6 0 13 169 21 779.6 0 21 170 10 779.6 0 10 171 56 779.6 0 56 172 5 779.6 0 5 173 8 779.6 0 8 174 11 779.6 0 11 175 7 779.6 0 7 176 7 779.6 0 7 177 7 779.6 0 7 178 8 779.6 0 8 179 10 779.6 0 10 180 8 779.6 0 8 181 13 779.6 0 13 182 16 779.6 0 16 183 12 779.6 0 12 184 9 779.6 0 9 185 13 779.6 0 13 186 14 779.6 0 14 187 10 779.6 0 10 188 12 779.6 0 12 189 14 779.6 0 14 190 25 779.6 0 25 191 179 779.6 0 179 192 72 779.6 0 72 193 69 779.6 0 69 194 53 779.6 0 53 195 112 779.6 0 112 196 319 779.6 0 319 197 232 779.6 0 232 198 10 779.6 0 10 199 11 779.6 0 11 200 7 779.6 0 7 201 5 779.6 0 5 202 2 779.6 0 2 203 5 779.6 0 5 204 5 779.6 0 5 205 9 779.6 0 9 206 11 779.6 0 11 207 9 779.6 0 9 208 4 779.6 0 4 209 7 779.6 0 7 210 13 779.6 0 13 211 12 779.6 0 12 212 17 779.6 0 17 213 35 779.6 0 35 214 43 779.6 0 43 215 29 779.6 0 29 216 25 779.6 0 25 217 24 779.6 0 24 218 33 779.6 0 33 219 42 779.6 0 42 220 12 779.6 0 12 221 11 779.6 0 11 222 3 779.6 0 3 223 7 779.6 0 7 224 10 779.6 0 10 225 13 779.6 0 13 226 17 779.6 0 17 227 13 779.6 0 13 228 12 779.6 0 12 229 22 779.6 0 22 230 35 779.6 0 35 231 33 779.6 0 33 232 7 779.6 0 7 233 11 779.6 0 11 234 141 779.6 0 141 235 35 779.6 0 35 236 74 779.6 0 74 237 34 779.6 0 34 238 35 779.6 0 35 239 110 779.6 0 110 240 36 779.6 0 36 241 7 779.6 0 7 242 4 779.6 0 4 243 33 779.6 0 33 244 52 779.6 0 52 245 141 779.6 0 141 246 139 779.6 0 139 247 44 779.6 0 44 248 25 779.6 0 25 249 18 779.6 0 18 250 14 779.6 0 14 251 8 779.6 0 8 252 28 779.6 0 28 253 89 779.6 0 89 254 45 779.6 0 45 255 51 779.6 0 51 256 52 779.6 0 52 257 88 779.6 0 88 258 74 779.6 0 74 259 46 779.6 0 46 260 92 779.6 0 92 261 95 779.6 0 95 262 45 779.6 0 45 263 31 779.6 0 31 264 9 779.6 0 9 265 11 779.6 0 11 266 22 779.6 0 22 267 43 779.6 0 43 268 27 779.6 0 27 269 75 779.6 0 75 270 25 779.6 0 25 271 14 779.6 0 14 272 12 779.6 0 12 273 15 779.6 0 15 274 11 779.6 0 11 275 13 779.6 0 13 276 8 779.6 0 8 277 9 779.6 0 9 278 17 779.6 0 17 279 55 779.6 0 55 280 46 779.6 0 46 281 33 779.6 0 33 282 29 779.6 0 29 283 16 779.6 0 16 284 42 779.6 0 42 285 44 779.6 0 44 286 33 779.6 0 33 287 63 779.6 0 63 288 83 779.6 0 83 289 110 779.6 0 110 290 19 779.6 0 19 291 20 779.6 0 20 292 72 779.6 0 72 293 119 779.6 0 119 294 50 779.6 0 50 295 39 779.6 0 39 296 39 779.6 0 39 297 34 779.6 0 34 298 25 779.6 0 25 299 15 779.6 0 15 300 42 779.6 0 42 301 31 779.6 0 31 Finished in 14.13 s (51 us/read; 1.18 M reads/minute). === Summary === Total reads processed: 278,195 Reads with adapters: 222,562 (80.0%) Reads that were too short: 17 (0.0%) Reads written (passing filters): 222,545 (80.0%) Total basepairs processed: 80,493,098 bp Total written (filtered): 60,542,964 bp (75.2%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 222562 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 10 0.0 3 0 0 0 6 4 18 143 0.0 3 0 0 119 16 8 19 3576 0.0 3 0 3412 115 22 27 20 217425 0.0 4 210745 5597 774 223 86 21 521 0.0 4 112 381 17 6 5 22 563 0.0 4 490 27 38 4 4 23 167 0.0 4 3 3 2 135 24 24 12 0.0 4 3 0 2 2 5 25 1 0.0 4 1 26 1 0.0 4 1 27 2 0.0 4 1 0 0 0 1 29 2 0.0 4 2 31 1 0.0 4 0 0 0 0 1 34 1 0.0 4 1 35 3 0.0 4 1 0 0 0 2 38 1 0.0 4 1 39 1 0.0 4 1 43 1 0.0 4 1 46 1 0.0 4 1 47 1 0.0 4 1 51 1 0.0 4 0 0 0 0 1 56 1 0.0 4 0 0 0 0 1 68 2 0.0 4 0 0 0 0 2 86 3 0.0 4 0 0 0 0 3 126 1 0.0 4 0 0 0 0 1 127 86 0.0 4 0 0 0 0 86 129 1 0.0 4 0 0 0 0 1 130 2 0.0 4 0 0 0 0 2 135 7 0.0 4 0 0 0 0 7 153 3 0.0 4 0 0 0 0 3 155 8 0.0 4 0 0 0 0 8 168 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 233 1 0.0 4 0 0 0 0 1 242 1 0.0 4 0 0 0 0 1 256 1 0.0 4 0 0 0 0 1 260 4 0.0 4 0 0 0 0 4 264 1 0.0 4 0 0 0 0 1 292 1 0.0 4 0 0 0 0 1 293 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 8.93 s (40 us/read; 1.50 M reads/minute). === Summary === Total reads processed: 222,545 Reads with adapters: 20,472 (9.2%) Reads written (passing filters): 222,545 (100.0%) Total basepairs processed: 60,542,964 bp Total written (filtered): 60,312,705 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 20472 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.5% C: 2.2% G: 93.3% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 18 3477.3 0 18 4 24 869.3 0 24 5 2 217.3 0 2 6 1 54.3 0 1 8 8 3.4 0 8 9 1437 0.8 0 116 1321 10 3744 0.2 1 114 3630 11 15107 0.1 1 32 15075 12 25 0.0 1 0 25 13 23 0.0 1 0 23 14 2 0.0 1 0 2 15 3 0.0 1 0 3 16 1 0.0 1 0 1 70 1 0.0 1 0 1 114 2 0.0 1 1 1 147 2 0.0 1 2 150 37 0.0 1 32 5 151 5 0.0 1 5 152 4 0.0 1 4 153 1 0.0 1 1 169 2 0.0 1 2 170 1 0.0 1 1 189 5 0.0 1 1 4 191 2 0.0 1 0 2 208 10 0.0 1 9 1 209 1 0.0 1 1 210 1 0.0 1 1 258 1 0.0 1 1 268 1 0.0 1 1 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.