This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTTCT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 102.23 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 239,874 (7.5%) Reads that were too short: 4,667 (0.1%) Reads written (passing filters): 235,207 (7.4%) Total basepairs processed: 961,105,341 bp Total written (filtered): 67,696,475 bp (7.0%) === Adapter 1 === Sequence: GGTTCT; Type: regular 5'; Length: 6; Trimmed: 239874 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1344 779.6 0 1344 7 4481 779.6 0 4481 8 4499 779.6 0 4499 9 213109 779.6 0 213109 10 402 779.6 0 402 11 10 779.6 0 10 12 12 779.6 0 12 13 7 779.6 0 7 14 2 779.6 0 2 15 11 779.6 0 11 16 6 779.6 0 6 17 8 779.6 0 8 18 6 779.6 0 6 19 13 779.6 0 13 20 24 779.6 0 24 21 24 779.6 0 24 22 35 779.6 0 35 23 384 779.6 0 384 24 556 779.6 0 556 25 932 779.6 0 932 26 7 779.6 0 7 27 7 779.6 0 7 28 3 779.6 0 3 29 9 779.6 0 9 30 9 779.6 0 9 31 9 779.6 0 9 32 3 779.6 0 3 33 2 779.6 0 2 34 8 779.6 0 8 35 8 779.6 0 8 36 16 779.6 0 16 37 1 779.6 0 1 38 10 779.6 0 10 39 8 779.6 0 8 40 23 779.6 0 23 41 9 779.6 0 9 42 7 779.6 0 7 43 3 779.6 0 3 44 6 779.6 0 6 45 6 779.6 0 6 46 5 779.6 0 5 47 2 779.6 0 2 48 5 779.6 0 5 49 4 779.6 0 4 50 40 779.6 0 40 51 6 779.6 0 6 52 17 779.6 0 17 53 120 779.6 0 120 54 7 779.6 0 7 55 12 779.6 0 12 56 7 779.6 0 7 57 10 779.6 0 10 58 4 779.6 0 4 59 1 779.6 0 1 60 6 779.6 0 6 61 3 779.6 0 3 62 7 779.6 0 7 63 7 779.6 0 7 64 9 779.6 0 9 65 14 779.6 0 14 66 3 779.6 0 3 67 4 779.6 0 4 68 16 779.6 0 16 69 42 779.6 0 42 70 5 779.6 0 5 71 27 779.6 0 27 72 11 779.6 0 11 73 38 779.6 0 38 74 63 779.6 0 63 75 334 779.6 0 334 76 8 779.6 0 8 77 6 779.6 0 6 78 7 779.6 0 7 79 3 779.6 0 3 80 10 779.6 0 10 81 14 779.6 0 14 82 17 779.6 0 17 83 15 779.6 0 15 84 17 779.6 0 17 85 14 779.6 0 14 86 25 779.6 0 25 87 23 779.6 0 23 88 587 779.6 0 587 89 456 779.6 0 456 90 312 779.6 0 312 91 4 779.6 0 4 92 4 779.6 0 4 93 2 779.6 0 2 94 5 779.6 0 5 95 15 779.6 0 15 96 34 779.6 0 34 97 26 779.6 0 26 98 20 779.6 0 20 99 15 779.6 0 15 100 32 779.6 0 32 101 219 779.6 0 219 102 293 779.6 0 293 103 359 779.6 0 359 104 23 779.6 0 23 105 11 779.6 0 11 106 11 779.6 0 11 107 4 779.6 0 4 108 2 779.6 0 2 109 9 779.6 0 9 110 13 779.6 0 13 111 15 779.6 0 15 112 39 779.6 0 39 113 66 779.6 0 66 114 108 779.6 0 108 115 19 779.6 0 19 116 23 779.6 0 23 117 35 779.6 0 35 118 23 779.6 0 23 119 25 779.6 0 25 120 38 779.6 0 38 121 16 779.6 0 16 122 30 779.6 0 30 123 29 779.6 0 29 124 36 779.6 0 36 125 46 779.6 0 46 126 87 779.6 0 87 127 1357 779.6 0 1357 128 2190 779.6 0 2190 129 955 779.6 0 955 130 10 779.6 0 10 131 10 779.6 0 10 132 6 779.6 0 6 133 10 779.6 0 10 134 7 779.6 0 7 135 40 779.6 0 40 136 10 779.6 0 10 137 5 779.6 0 5 138 6 779.6 0 6 139 5 779.6 0 5 140 3 779.6 0 3 141 5 779.6 0 5 142 5 779.6 0 5 143 8 779.6 0 8 144 8 779.6 0 8 145 29 779.6 0 29 146 13 779.6 0 13 147 22 779.6 0 22 148 48 779.6 0 48 149 72 779.6 0 72 150 3 779.6 0 3 151 4 779.6 0 4 152 3 779.6 0 3 153 3 779.6 0 3 154 5 779.6 0 5 155 4 779.6 0 4 156 4 779.6 0 4 157 4 779.6 0 4 158 3 779.6 0 3 159 2 779.6 0 2 160 5 779.6 0 5 161 9 779.6 0 9 162 2 779.6 0 2 163 2 779.6 0 2 164 5 779.6 0 5 165 5 779.6 0 5 166 5 779.6 0 5 167 5 779.6 0 5 168 6 779.6 0 6 169 6 779.6 0 6 170 3 779.6 0 3 171 3 779.6 0 3 172 4 779.6 0 4 173 1 779.6 0 1 174 6 779.6 0 6 175 2 779.6 0 2 176 8 779.6 0 8 177 2 779.6 0 2 178 1 779.6 0 1 180 4 779.6 0 4 181 9 779.6 0 9 182 8 779.6 0 8 183 8 779.6 0 8 184 4 779.6 0 4 185 3 779.6 0 3 186 4 779.6 0 4 187 7 779.6 0 7 188 5 779.6 0 5 189 8 779.6 0 8 190 4 779.6 0 4 191 3 779.6 0 3 192 4 779.6 0 4 193 23 779.6 0 23 194 15 779.6 0 15 195 20 779.6 0 20 196 19 779.6 0 19 197 2 779.6 0 2 198 3 779.6 0 3 199 4 779.6 0 4 200 7 779.6 0 7 201 6 779.6 0 6 202 3 779.6 0 3 203 7 779.6 0 7 204 3 779.6 0 3 205 7 779.6 0 7 206 12 779.6 0 12 207 13 779.6 0 13 208 10 779.6 0 10 209 9 779.6 0 9 210 4 779.6 0 4 211 6 779.6 0 6 212 7 779.6 0 7 213 6 779.6 0 6 214 9 779.6 0 9 215 14 779.6 0 14 216 3 779.6 0 3 217 7 779.6 0 7 218 2 779.6 0 2 219 4 779.6 0 4 220 4 779.6 0 4 221 3 779.6 0 3 222 11 779.6 0 11 223 5 779.6 0 5 224 11 779.6 0 11 225 12 779.6 0 12 226 7 779.6 0 7 227 11 779.6 0 11 228 9 779.6 0 9 229 8 779.6 0 8 230 3 779.6 0 3 231 6 779.6 0 6 232 6 779.6 0 6 233 9 779.6 0 9 234 4 779.6 0 4 235 6 779.6 0 6 236 33 779.6 0 33 237 9 779.6 0 9 238 6 779.6 0 6 239 3 779.6 0 3 240 9 779.6 0 9 241 6 779.6 0 6 242 12 779.6 0 12 243 14 779.6 0 14 244 6 779.6 0 6 245 15 779.6 0 15 246 12 779.6 0 12 247 26 779.6 0 26 248 8 779.6 0 8 249 16 779.6 0 16 250 19 779.6 0 19 251 16 779.6 0 16 252 107 779.6 0 107 253 64 779.6 0 64 254 67 779.6 0 67 255 68 779.6 0 68 256 22 779.6 0 22 257 31 779.6 0 31 258 49 779.6 0 49 259 142 779.6 0 142 260 808 779.6 0 808 261 1424 779.6 0 1424 262 848 779.6 0 848 263 9 779.6 0 9 264 6 779.6 0 6 265 8 779.6 0 8 266 9 779.6 0 9 267 7 779.6 0 7 268 4 779.6 0 4 269 10 779.6 0 10 270 2 779.6 0 2 272 5 779.6 0 5 273 4 779.6 0 4 274 5 779.6 0 5 275 8 779.6 0 8 276 7 779.6 0 7 277 11 779.6 0 11 278 8 779.6 0 8 279 168 779.6 0 168 280 136 779.6 0 136 281 62 779.6 0 62 282 6 779.6 0 6 283 5 779.6 0 5 284 3 779.6 0 3 285 4 779.6 0 4 286 4 779.6 0 4 287 3 779.6 0 3 288 5 779.6 0 5 289 4 779.6 0 4 290 3 779.6 0 3 291 4 779.6 0 4 292 8 779.6 0 8 293 7 779.6 0 7 294 20 779.6 0 20 295 12 779.6 0 12 296 2 779.6 0 2 297 5 779.6 0 5 298 5 779.6 0 5 299 5 779.6 0 5 300 7 779.6 0 7 301 5 779.6 0 5 Finished in 11.07 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 235,207 Reads with adapters: 219,838 (93.5%) Reads that were too short: 1 (0.0%) Reads written (passing filters): 219,837 (93.5%) Total basepairs processed: 67,696,475 bp Total written (filtered): 60,461,224 bp (89.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 219838 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 16 0.0 2 0 0 0 16 15 66 0.0 3 0 0 62 4 16 1729 0.0 3 0 1687 40 2 17 217231 0.0 3 212479 4634 104 14 18 475 0.0 3 22 429 19 5 19 127 0.0 3 1 0 99 27 20 154 0.0 3 0 0 0 154 21 1 0.0 3 0 0 0 1 23 1 0.0 3 1 25 1 0.0 3 1 26 1 0.0 3 1 30 1 0.0 3 1 31 1 0.0 3 1 32 1 0.0 3 1 33 1 0.0 3 1 36 2 0.0 3 2 40 1 0.0 3 0 0 1 50 1 0.0 3 0 0 0 1 63 1 0.0 3 0 0 0 1 64 10 0.0 3 0 0 0 10 67 5 0.0 3 0 0 0 5 73 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 100 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 106 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 138 1 0.0 3 1 139 1 0.0 3 0 0 0 1 166 1 0.0 3 0 0 0 1 175 1 0.0 3 0 0 0 1 182 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 9.73 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 219,837 Reads with adapters: 20,024 (9.1%) Reads written (passing filters): 219,837 (100.0%) Total basepairs processed: 60,461,224 bp Total written (filtered): 60,401,079 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 20024 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 36.0% C: 7.2% G: 56.2% T: 0.7% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 19952 3435.0 0 19952 4 71 858.7 0 71 5 1 214.7 0 1