This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TGCGTG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 102.76 s (32 us/read; 1.86 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 55,373 (1.7%) Reads that were too short: 12,298 (0.4%) Reads written (passing filters): 43,075 (1.3%) Total basepairs processed: 961,105,341 bp Total written (filtered): 11,696,641 bp (1.2%) === Adapter 1 === Sequence: TGCGTG; Type: regular 5'; Length: 6; Trimmed: 55373 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 5047 779.6 0 5047 7 18747 779.6 0 18747 8 9249 779.6 0 9249 9 31 779.6 0 31 10 200 779.6 0 200 11 11 779.6 0 11 12 10 779.6 0 10 13 18 779.6 0 18 14 20 779.6 0 20 15 13 779.6 0 13 16 14 779.6 0 14 17 77 779.6 0 77 18 61 779.6 0 61 19 38 779.6 0 38 20 13 779.6 0 13 21 10 779.6 0 10 22 12 779.6 0 12 23 10 779.6 0 10 24 13 779.6 0 13 25 12 779.6 0 12 26 10 779.6 0 10 27 10 779.6 0 10 28 11 779.6 0 11 29 24 779.6 0 24 30 15 779.6 0 15 31 11 779.6 0 11 32 17 779.6 0 17 33 8 779.6 0 8 34 9 779.6 0 9 35 14 779.6 0 14 36 15 779.6 0 15 37 8 779.6 0 8 38 17 779.6 0 17 39 10 779.6 0 10 40 14 779.6 0 14 41 14 779.6 0 14 42 31 779.6 0 31 43 14 779.6 0 14 44 7 779.6 0 7 45 9 779.6 0 9 46 18 779.6 0 18 47 11 779.6 0 11 48 43 779.6 0 43 49 64 779.6 0 64 50 15 779.6 0 15 51 23 779.6 0 23 52 12 779.6 0 12 53 63 779.6 0 63 54 19 779.6 0 19 55 17 779.6 0 17 56 28 779.6 0 28 57 33 779.6 0 33 58 31 779.6 0 31 59 16 779.6 0 16 60 59 779.6 0 59 61 34 779.6 0 34 62 32 779.6 0 32 63 36 779.6 0 36 64 41 779.6 0 41 65 19 779.6 0 19 66 21 779.6 0 21 67 59 779.6 0 59 68 19 779.6 0 19 69 14 779.6 0 14 70 32 779.6 0 32 71 22 779.6 0 22 72 53 779.6 0 53 73 451 779.6 0 451 74 690 779.6 0 690 75 436 779.6 0 436 76 35 779.6 0 35 77 358 779.6 0 358 78 815 779.6 0 815 79 683 779.6 0 683 80 9 779.6 0 9 81 10 779.6 0 10 82 14 779.6 0 14 83 8 779.6 0 8 84 8 779.6 0 8 85 11 779.6 0 11 86 16 779.6 0 16 87 9 779.6 0 9 88 12 779.6 0 12 89 15 779.6 0 15 90 6 779.6 0 6 91 16 779.6 0 16 92 12 779.6 0 12 93 10 779.6 0 10 94 12 779.6 0 12 95 9 779.6 0 9 96 11 779.6 0 11 97 17 779.6 0 17 98 10 779.6 0 10 99 10 779.6 0 10 100 6 779.6 0 6 101 10 779.6 0 10 102 8 779.6 0 8 103 8 779.6 0 8 104 17 779.6 0 17 105 24 779.6 0 24 106 37 779.6 0 37 107 25 779.6 0 25 108 279 779.6 0 279 109 8 779.6 0 8 110 12 779.6 0 12 111 5 779.6 0 5 112 12 779.6 0 12 113 30 779.6 0 30 114 22 779.6 0 22 115 27 779.6 0 27 116 15 779.6 0 15 117 15 779.6 0 15 118 15 779.6 0 15 119 10 779.6 0 10 120 21 779.6 0 21 121 20 779.6 0 20 122 17 779.6 0 17 123 16 779.6 0 16 124 12 779.6 0 12 125 22 779.6 0 22 126 21 779.6 0 21 127 28 779.6 0 28 128 221 779.6 0 221 129 212 779.6 0 212 130 329 779.6 0 329 131 45 779.6 0 45 132 26 779.6 0 26 133 31 779.6 0 31 134 189 779.6 0 189 135 75 779.6 0 75 136 238 779.6 0 238 137 28 779.6 0 28 138 32 779.6 0 32 139 19 779.6 0 19 140 24 779.6 0 24 141 26 779.6 0 26 142 11 779.6 0 11 143 22 779.6 0 22 144 78 779.6 0 78 145 153 779.6 0 153 146 204 779.6 0 204 147 145 779.6 0 145 148 310 779.6 0 310 149 13 779.6 0 13 150 39 779.6 0 39 151 13 779.6 0 13 152 12 779.6 0 12 153 12 779.6 0 12 154 9 779.6 0 9 155 37 779.6 0 37 156 19 779.6 0 19 157 74 779.6 0 74 158 17 779.6 0 17 159 28 779.6 0 28 160 36 779.6 0 36 161 54 779.6 0 54 162 14 779.6 0 14 163 10 779.6 0 10 164 61 779.6 0 61 165 50 779.6 0 50 166 64 779.6 0 64 167 32 779.6 0 32 168 10 779.6 0 10 169 12 779.6 0 12 170 16 779.6 0 16 171 17 779.6 0 17 172 18 779.6 0 18 173 9 779.6 0 9 174 11 779.6 0 11 175 16 779.6 0 16 176 5 779.6 0 5 177 6 779.6 0 6 178 15 779.6 0 15 179 17 779.6 0 17 180 12 779.6 0 12 181 23 779.6 0 23 182 25 779.6 0 25 183 16 779.6 0 16 184 63 779.6 0 63 185 206 779.6 0 206 186 149 779.6 0 149 187 22 779.6 0 22 188 11 779.6 0 11 189 9 779.6 0 9 190 13 779.6 0 13 191 13 779.6 0 13 192 17 779.6 0 17 193 11 779.6 0 11 194 10 779.6 0 10 195 33 779.6 0 33 196 55 779.6 0 55 197 38 779.6 0 38 198 12 779.6 0 12 199 10 779.6 0 10 200 10 779.6 0 10 201 10 779.6 0 10 202 9 779.6 0 9 203 7 779.6 0 7 204 27 779.6 0 27 205 18 779.6 0 18 206 25 779.6 0 25 207 18 779.6 0 18 208 16 779.6 0 16 209 40 779.6 0 40 210 8 779.6 0 8 211 13 779.6 0 13 212 18 779.6 0 18 213 21 779.6 0 21 214 8 779.6 0 8 215 34 779.6 0 34 216 32 779.6 0 32 217 63 779.6 0 63 218 18 779.6 0 18 219 31 779.6 0 31 220 16 779.6 0 16 221 21 779.6 0 21 222 9 779.6 0 9 223 16 779.6 0 16 224 33 779.6 0 33 225 20 779.6 0 20 226 22 779.6 0 22 227 19 779.6 0 19 228 21 779.6 0 21 229 31 779.6 0 31 230 31 779.6 0 31 231 45 779.6 0 45 232 47 779.6 0 47 233 67 779.6 0 67 234 135 779.6 0 135 235 164 779.6 0 164 236 2447 779.6 0 2447 237 1277 779.6 0 1277 238 4832 779.6 0 4832 239 26 779.6 0 26 240 11 779.6 0 11 241 10 779.6 0 10 242 7 779.6 0 7 243 6 779.6 0 6 244 15 779.6 0 15 245 17 779.6 0 17 246 43 779.6 0 43 247 58 779.6 0 58 248 33 779.6 0 33 249 16 779.6 0 16 250 48 779.6 0 48 251 33 779.6 0 33 252 33 779.6 0 33 253 82 779.6 0 82 254 79 779.6 0 79 255 93 779.6 0 93 256 47 779.6 0 47 257 15 779.6 0 15 258 8 779.6 0 8 259 6 779.6 0 6 260 5 779.6 0 5 261 4 779.6 0 4 262 6 779.6 0 6 263 6 779.6 0 6 264 6 779.6 0 6 265 17 779.6 0 17 266 17 779.6 0 17 267 13 779.6 0 13 268 12 779.6 0 12 269 10 779.6 0 10 270 10 779.6 0 10 271 16 779.6 0 16 272 21 779.6 0 21 273 18 779.6 0 18 274 11 779.6 0 11 275 10 779.6 0 10 276 24 779.6 0 24 277 37 779.6 0 37 278 44 779.6 0 44 279 50 779.6 0 50 280 30 779.6 0 30 281 96 779.6 0 96 282 166 779.6 0 166 283 210 779.6 0 210 284 129 779.6 0 129 285 30 779.6 0 30 286 124 779.6 0 124 287 6 779.6 0 6 288 9 779.6 0 9 289 7 779.6 0 7 290 17 779.6 0 17 291 23 779.6 0 23 292 52 779.6 0 52 293 24 779.6 0 24 294 27 779.6 0 27 295 33 779.6 0 33 296 98 779.6 0 98 297 141 779.6 0 141 298 305 779.6 0 305 299 42 779.6 0 42 300 31 779.6 0 31 301 16 779.6 0 16 Finished in 2.56 s (59 us/read; 1.01 M reads/minute). === Summary === Total reads processed: 43,075 Reads with adapters: 14,155 (32.9%) Reads that were too short: 25 (0.1%) Reads written (passing filters): 14,130 (32.8%) Total basepairs processed: 11,696,641 bp Total written (filtered): 3,838,200 bp (32.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 14155 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 7 0.0 3 0 0 6 1 19 161 0.0 3 0 144 11 4 2 20 9324 0.0 4 8971 301 40 6 6 21 105 0.0 4 19 83 2 0 1 22 4315 0.0 4 4147 147 13 6 2 23 25 0.0 4 5 10 0 8 2 24 1 0.0 4 0 0 0 0 1 25 6 0.0 4 0 0 0 5 1 34 1 0.0 4 0 1 48 1 0.0 4 0 0 0 0 1 57 11 0.0 4 0 0 0 0 11 59 1 0.0 4 0 0 1 61 1 0.0 4 0 0 0 0 1 83 1 0.0 4 0 0 0 0 1 87 1 0.0 4 0 0 0 0 1 91 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 1 100 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 1 117 1 0.0 4 0 0 0 0 1 126 3 0.0 4 0 0 0 0 3 127 168 0.0 4 0 0 0 0 168 128 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 0 1 138 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 155 1 0.0 4 0 0 0 0 1 163 1 0.0 4 0 0 0 0 1 184 1 0.0 4 0 0 0 0 1 185 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 225 1 0.0 4 0 0 0 0 1 227 1 0.0 4 0 0 0 0 1 229 2 0.0 4 0 0 0 0 2 234 1 0.0 4 0 0 0 0 1 236 1 0.0 4 0 0 0 0 1 249 1 0.0 4 0 0 0 0 1 257 1 0.0 4 0 0 0 0 1 283 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.63 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 14,130 Reads with adapters: 2,794 (19.8%) Reads written (passing filters): 14,130 (100.0%) Total basepairs processed: 3,838,200 bp Total written (filtered): 3,788,872 bp (98.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 2794 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.0% C: 1.4% G: 93.1% T: 1.4% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 220.8 0 1 4 1 55.2 0 1 9 1 0.1 0 0 1 10 36 0.0 1 3 33 11 2673 0.0 1 5 2668 12 5 0.0 1 0 5 151 3 0.0 1 3 224 3 0.0 1 3 231 1 0.0 1 1 258 2 0.0 1 2 259 64 0.0 1 59 5 260 3 0.0 1 3 261 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.