This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AATAGG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 102.50 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 33,361 (1.0%) Reads that were too short: 10,016 (0.3%) Reads written (passing filters): 23,345 (0.7%) Total basepairs processed: 961,105,341 bp Total written (filtered): 5,304,392 bp (0.6%) === Adapter 1 === Sequence: AATAGG; Type: regular 5'; Length: 6; Trimmed: 33361 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 44 779.6 0 44 7 141 779.6 0 141 8 9077 779.6 0 9077 9 142 779.6 0 142 10 49 779.6 0 49 11 2 779.6 0 2 12 1 779.6 0 1 13 2 779.6 0 2 14 2 779.6 0 2 15 3 779.6 0 3 16 2 779.6 0 2 17 3 779.6 0 3 18 6 779.6 0 6 19 6 779.6 0 6 20 6 779.6 0 6 21 2 779.6 0 2 22 5 779.6 0 5 23 1 779.6 0 1 24 2 779.6 0 2 25 8 779.6 0 8 26 3 779.6 0 3 27 3 779.6 0 3 28 11 779.6 0 11 29 28 779.6 0 28 30 37 779.6 0 37 31 130 779.6 0 130 32 351 779.6 0 351 33 5 779.6 0 5 34 7 779.6 0 7 35 23 779.6 0 23 36 29 779.6 0 29 37 9 779.6 0 9 38 7 779.6 0 7 39 9 779.6 0 9 40 3 779.6 0 3 41 3 779.6 0 3 42 8 779.6 0 8 43 13 779.6 0 13 44 16 779.6 0 16 45 138 779.6 0 138 46 273 779.6 0 273 47 176 779.6 0 176 48 2 779.6 0 2 49 5 779.6 0 5 50 4 779.6 0 4 51 6 779.6 0 6 52 7 779.6 0 7 53 3 779.6 0 3 54 4 779.6 0 4 55 7 779.6 0 7 56 5 779.6 0 5 57 4 779.6 0 4 58 4 779.6 0 4 59 5 779.6 0 5 60 6 779.6 0 6 61 5 779.6 0 5 62 1 779.6 0 1 63 8 779.6 0 8 64 2 779.6 0 2 65 2 779.6 0 2 66 12 779.6 0 12 67 12 779.6 0 12 68 52 779.6 0 52 69 31 779.6 0 31 70 420 779.6 0 420 71 31 779.6 0 31 72 9 779.6 0 9 73 8 779.6 0 8 75 2 779.6 0 2 76 20 779.6 0 20 77 83 779.6 0 83 78 48 779.6 0 48 79 26 779.6 0 26 80 25 779.6 0 25 81 17 779.6 0 17 82 7 779.6 0 7 83 18 779.6 0 18 84 20 779.6 0 20 85 4 779.6 0 4 86 4 779.6 0 4 87 11 779.6 0 11 88 15 779.6 0 15 89 17 779.6 0 17 90 64 779.6 0 64 91 50 779.6 0 50 92 138 779.6 0 138 93 348 779.6 0 348 94 168 779.6 0 168 95 315 779.6 0 315 96 207 779.6 0 207 97 1203 779.6 0 1203 98 92 779.6 0 92 99 53 779.6 0 53 100 149 779.6 0 149 101 31 779.6 0 31 102 11 779.6 0 11 103 14 779.6 0 14 104 18 779.6 0 18 105 9 779.6 0 9 106 29 779.6 0 29 107 28 779.6 0 28 108 7 779.6 0 7 109 43 779.6 0 43 110 82 779.6 0 82 111 27 779.6 0 27 112 35 779.6 0 35 113 168 779.6 0 168 114 727 779.6 0 727 115 1204 779.6 0 1204 116 228 779.6 0 228 117 51 779.6 0 51 118 91 779.6 0 91 119 76 779.6 0 76 120 32 779.6 0 32 121 35 779.6 0 35 122 46 779.6 0 46 123 40 779.6 0 40 124 35 779.6 0 35 125 62 779.6 0 62 126 30 779.6 0 30 127 20 779.6 0 20 128 137 779.6 0 137 129 94 779.6 0 94 130 102 779.6 0 102 131 152 779.6 0 152 132 63 779.6 0 63 133 73 779.6 0 73 134 109 779.6 0 109 135 302 779.6 0 302 136 166 779.6 0 166 137 241 779.6 0 241 138 135 779.6 0 135 139 207 779.6 0 207 140 247 779.6 0 247 141 33 779.6 0 33 142 51 779.6 0 51 143 77 779.6 0 77 144 120 779.6 0 120 145 61 779.6 0 61 146 38 779.6 0 38 147 24 779.6 0 24 148 13 779.6 0 13 149 15 779.6 0 15 150 32 779.6 0 32 151 32 779.6 0 32 152 43 779.6 0 43 153 32 779.6 0 32 154 53 779.6 0 53 155 72 779.6 0 72 156 34 779.6 0 34 157 65 779.6 0 65 158 34 779.6 0 34 159 29 779.6 0 29 160 46 779.6 0 46 161 42 779.6 0 42 162 68 779.6 0 68 163 79 779.6 0 79 164 102 779.6 0 102 165 69 779.6 0 69 166 49 779.6 0 49 167 92 779.6 0 92 168 130 779.6 0 130 169 121 779.6 0 121 170 56 779.6 0 56 171 81 779.6 0 81 172 24 779.6 0 24 173 43 779.6 0 43 174 38 779.6 0 38 175 24 779.6 0 24 176 19 779.6 0 19 177 41 779.6 0 41 178 92 779.6 0 92 179 93 779.6 0 93 180 93 779.6 0 93 181 67 779.6 0 67 182 53 779.6 0 53 183 70 779.6 0 70 184 84 779.6 0 84 185 57 779.6 0 57 186 28 779.6 0 28 187 29 779.6 0 29 188 28 779.6 0 28 189 78 779.6 0 78 190 207 779.6 0 207 191 178 779.6 0 178 192 55 779.6 0 55 193 29 779.6 0 29 194 21 779.6 0 21 195 64 779.6 0 64 196 51 779.6 0 51 197 70 779.6 0 70 198 63 779.6 0 63 199 23 779.6 0 23 200 25 779.6 0 25 201 53 779.6 0 53 202 175 779.6 0 175 203 413 779.6 0 413 204 616 779.6 0 616 205 100 779.6 0 100 206 30 779.6 0 30 207 44 779.6 0 44 208 55 779.6 0 55 209 171 779.6 0 171 210 279 779.6 0 279 211 157 779.6 0 157 212 126 779.6 0 126 213 48 779.6 0 48 214 77 779.6 0 77 215 131 779.6 0 131 216 97 779.6 0 97 217 81 779.6 0 81 218 57 779.6 0 57 219 22 779.6 0 22 220 36 779.6 0 36 221 93 779.6 0 93 222 50 779.6 0 50 223 104 779.6 0 104 224 172 779.6 0 172 225 396 779.6 0 396 226 419 779.6 0 419 227 468 779.6 0 468 228 106 779.6 0 106 229 39 779.6 0 39 230 21 779.6 0 21 231 36 779.6 0 36 232 85 779.6 0 85 233 85 779.6 0 85 234 146 779.6 0 146 235 328 779.6 0 328 236 219 779.6 0 219 237 144 779.6 0 144 238 76 779.6 0 76 239 21 779.6 0 21 240 32 779.6 0 32 241 58 779.6 0 58 242 69 779.6 0 69 243 65 779.6 0 65 244 68 779.6 0 68 245 37 779.6 0 37 246 24 779.6 0 24 247 12 779.6 0 12 248 14 779.6 0 14 249 34 779.6 0 34 250 65 779.6 0 65 251 74 779.6 0 74 252 252 779.6 0 252 253 115 779.6 0 115 254 55 779.6 0 55 255 35 779.6 0 35 256 13 779.6 0 13 257 23 779.6 0 23 258 24 779.6 0 24 259 33 779.6 0 33 260 50 779.6 0 50 261 82 779.6 0 82 262 64 779.6 0 64 263 144 779.6 0 144 264 63 779.6 0 63 265 59 779.6 0 59 266 17 779.6 0 17 267 16 779.6 0 16 268 17 779.6 0 17 269 32 779.6 0 32 270 173 779.6 0 173 271 114 779.6 0 114 272 65 779.6 0 65 273 31 779.6 0 31 274 31 779.6 0 31 275 27 779.6 0 27 276 33 779.6 0 33 277 33 779.6 0 33 278 43 779.6 0 43 279 33 779.6 0 33 280 18 779.6 0 18 281 29 779.6 0 29 282 29 779.6 0 29 283 59 779.6 0 59 284 49 779.6 0 49 285 38 779.6 0 38 286 31 779.6 0 31 287 10 779.6 0 10 288 34 779.6 0 34 289 79 779.6 0 79 290 133 779.6 0 133 291 175 779.6 0 175 292 54 779.6 0 54 293 39 779.6 0 39 294 48 779.6 0 48 295 88 779.6 0 88 296 335 779.6 0 335 297 292 779.6 0 292 298 141 779.6 0 141 299 197 779.6 0 197 300 134 779.6 0 134 301 52 779.6 0 52 Finished in 1.06 s (45 us/read; 1.32 M reads/minute). === Summary === Total reads processed: 23,345 Reads with adapters: 9,244 (39.6%) Reads that were too short: 6 (0.0%) Reads written (passing filters): 9,238 (39.6%) Total basepairs processed: 5,304,392 bp Total written (filtered): 2,544,553 bp (48.0%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 9244 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 16 65 0.0 3 0 63 2 17 9071 0.0 3 8945 123 3 18 31 0.0 3 6 19 5 1 19 26 0.0 3 5 0 21 20 12 0.0 3 0 0 0 12 22 1 0.0 3 0 1 28 1 0.0 3 0 0 0 1 52 1 0.0 3 0 0 0 1 56 2 0.0 3 0 0 0 2 61 1 0.0 3 0 0 1 64 1 0.0 3 0 0 0 1 78 2 0.0 3 0 0 0 2 81 7 0.0 3 0 0 0 7 88 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 99 4 0.0 3 0 0 0 4 100 1 0.0 3 0 0 0 1 113 3 0.0 3 0 0 0 3 146 3 0.0 3 0 0 0 3 149 2 0.0 3 0 0 0 2 152 1 0.0 3 0 0 0 1 163 1 0.0 3 0 0 0 1 164 1 0.0 3 0 0 0 1 176 1 0.0 3 0 0 0 1 179 1 0.0 3 0 0 0 1 190 1 0.0 3 0 0 0 1 242 1 0.0 3 0 0 0 1 243 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.38 s (41 us/read; 1.46 M reads/minute). === Summary === Total reads processed: 9,238 Reads with adapters: 92 (1.0%) Reads written (passing filters): 9,238 (100.0%) Total basepairs processed: 2,544,553 bp Total written (filtered): 2,544,276 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 92 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 10.9% C: 71.7% G: 15.2% T: 2.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 91 144.3 0 91 4 1 36.1 0 1