This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TAACAT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 101.52 s (32 us/read; 1.89 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 200,197 (6.3%) Reads that were too short: 102,680 (3.2%) Reads written (passing filters): 97,517 (3.1%) Total basepairs processed: 961,105,341 bp Total written (filtered): 20,089,130 bp (2.1%) === Adapter 1 === Sequence: TAACAT; Type: regular 5'; Length: 6; Trimmed: 200197 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 614 779.6 0 614 7 20903 779.6 0 20903 8 181 779.6 0 181 9 11094 779.6 0 11094 10 43 779.6 0 43 11 10 779.6 0 10 12 8 779.6 0 8 13 12 779.6 0 12 14 4 779.6 0 4 15 3 779.6 0 3 16 1 779.6 0 1 17 6 779.6 0 6 18 6 779.6 0 6 19 2 779.6 0 2 20 5 779.6 0 5 22 1 779.6 0 1 23 2 779.6 0 2 24 1 779.6 0 1 25 6 779.6 0 6 26 4 779.6 0 4 27 1 779.6 0 1 28 7 779.6 0 7 29 2 779.6 0 2 30 8 779.6 0 8 31 7 779.6 0 7 32 6 779.6 0 6 33 5 779.6 0 5 34 4 779.6 0 4 35 4 779.6 0 4 36 5 779.6 0 5 37 4 779.6 0 4 38 3 779.6 0 3 39 3 779.6 0 3 40 1 779.6 0 1 41 5 779.6 0 5 42 2 779.6 0 2 43 2 779.6 0 2 44 6 779.6 0 6 45 2 779.6 0 2 46 2 779.6 0 2 47 1 779.6 0 1 48 9 779.6 0 9 49 3 779.6 0 3 51 2 779.6 0 2 52 5 779.6 0 5 54 34 779.6 0 34 55 1926 779.6 0 1926 56 23 779.6 0 23 57 3 779.6 0 3 58 2 779.6 0 2 59 6 779.6 0 6 60 2 779.6 0 2 61 20 779.6 0 20 62 11 779.6 0 11 63 7 779.6 0 7 64 3 779.6 0 3 65 8 779.6 0 8 66 27 779.6 0 27 67 5 779.6 0 5 68 5 779.6 0 5 69 8 779.6 0 8 70 12 779.6 0 12 71 24 779.6 0 24 72 27 779.6 0 27 73 24 779.6 0 24 74 73 779.6 0 73 75 90 779.6 0 90 76 310 779.6 0 310 77 110 779.6 0 110 78 97 779.6 0 97 79 202 779.6 0 202 80 228 779.6 0 228 81 413 779.6 0 413 82 552 779.6 0 552 83 336 779.6 0 336 84 265 779.6 0 265 85 209 779.6 0 209 86 561 779.6 0 561 87 386 779.6 0 386 88 131 779.6 0 131 89 1068 779.6 0 1068 90 138 779.6 0 138 91 104 779.6 0 104 92 466 779.6 0 466 93 268 779.6 0 268 94 125 779.6 0 125 95 269 779.6 0 269 96 154 779.6 0 154 97 388 779.6 0 388 98 620 779.6 0 620 99 351 779.6 0 351 100 168 779.6 0 168 101 185 779.6 0 185 102 383 779.6 0 383 103 1031 779.6 0 1031 104 380 779.6 0 380 105 160 779.6 0 160 106 237 779.6 0 237 107 163 779.6 0 163 108 429 779.6 0 429 109 1111 779.6 0 1111 110 731 779.6 0 731 111 318 779.6 0 318 112 409 779.6 0 409 113 460 779.6 0 460 114 320 779.6 0 320 115 791 779.6 0 791 116 198 779.6 0 198 117 288 779.6 0 288 118 729 779.6 0 729 119 292 779.6 0 292 120 332 779.6 0 332 121 403 779.6 0 403 122 313 779.6 0 313 123 402 779.6 0 402 124 385 779.6 0 385 125 618 779.6 0 618 126 372 779.6 0 372 127 520 779.6 0 520 128 372 779.6 0 372 129 396 779.6 0 396 130 437 779.6 0 437 131 257 779.6 0 257 132 516 779.6 0 516 133 797 779.6 0 797 134 871 779.6 0 871 135 643 779.6 0 643 136 314 779.6 0 314 137 712 779.6 0 712 138 4898 779.6 0 4898 139 728 779.6 0 728 140 488 779.6 0 488 141 535 779.6 0 535 142 258 779.6 0 258 143 400 779.6 0 400 144 946 779.6 0 946 145 455 779.6 0 455 146 670 779.6 0 670 147 385 779.6 0 385 148 486 779.6 0 486 149 450 779.6 0 450 150 428 779.6 0 428 151 1069 779.6 0 1069 152 568 779.6 0 568 153 783 779.6 0 783 154 657 779.6 0 657 155 458 779.6 0 458 156 792 779.6 0 792 157 642 779.6 0 642 158 468 779.6 0 468 159 631 779.6 0 631 160 450 779.6 0 450 161 550 779.6 0 550 162 395 779.6 0 395 163 355 779.6 0 355 164 565 779.6 0 565 165 449 779.6 0 449 166 474 779.6 0 474 167 264 779.6 0 264 168 349 779.6 0 349 169 233 779.6 0 233 170 407 779.6 0 407 171 399 779.6 0 399 172 671 779.6 0 671 173 474 779.6 0 474 174 238 779.6 0 238 175 205 779.6 0 205 176 372 779.6 0 372 177 611 779.6 0 611 178 453 779.6 0 453 179 597 779.6 0 597 180 527 779.6 0 527 181 677 779.6 0 677 182 602 779.6 0 602 183 384 779.6 0 384 184 463 779.6 0 463 185 440 779.6 0 440 186 437 779.6 0 437 187 419 779.6 0 419 188 542 779.6 0 542 189 750 779.6 0 750 190 966 779.6 0 966 191 405 779.6 0 405 192 353 779.6 0 353 193 222 779.6 0 222 194 418 779.6 0 418 195 508 779.6 0 508 196 553 779.6 0 553 197 663 779.6 0 663 198 301 779.6 0 301 199 340 779.6 0 340 200 579 779.6 0 579 201 723 779.6 0 723 202 776 779.6 0 776 203 504 779.6 0 504 204 348 779.6 0 348 205 457 779.6 0 457 206 769 779.6 0 769 207 538 779.6 0 538 208 653 779.6 0 653 209 1290 779.6 0 1290 210 1023 779.6 0 1023 211 8559 779.6 0 8559 212 5475 779.6 0 5475 213 11732 779.6 0 11732 214 4682 779.6 0 4682 215 494 779.6 0 494 216 520 779.6 0 520 217 432 779.6 0 432 218 408 779.6 0 408 219 626 779.6 0 626 220 1001 779.6 0 1001 221 461 779.6 0 461 222 594 779.6 0 594 223 716 779.6 0 716 224 632 779.6 0 632 225 664 779.6 0 664 226 771 779.6 0 771 227 380 779.6 0 380 228 358 779.6 0 358 229 338 779.6 0 338 230 510 779.6 0 510 231 577 779.6 0 577 232 538 779.6 0 538 233 597 779.6 0 597 234 823 779.6 0 823 235 449 779.6 0 449 236 446 779.6 0 446 237 572 779.6 0 572 238 593 779.6 0 593 239 793 779.6 0 793 240 917 779.6 0 917 241 808 779.6 0 808 242 633 779.6 0 633 243 582 779.6 0 582 244 487 779.6 0 487 245 689 779.6 0 689 246 524 779.6 0 524 247 394 779.6 0 394 248 333 779.6 0 333 249 483 779.6 0 483 250 716 779.6 0 716 251 771 779.6 0 771 252 456 779.6 0 456 253 465 779.6 0 465 254 490 779.6 0 490 255 385 779.6 0 385 256 330 779.6 0 330 257 351 779.6 0 351 258 377 779.6 0 377 259 552 779.6 0 552 260 641 779.6 0 641 261 531 779.6 0 531 262 915 779.6 0 915 263 605 779.6 0 605 264 877 779.6 0 877 265 655 779.6 0 655 266 405 779.6 0 405 267 592 779.6 0 592 268 467 779.6 0 467 269 826 779.6 0 826 270 1431 779.6 0 1431 271 1227 779.6 0 1227 272 1413 779.6 0 1413 273 775 779.6 0 775 274 1066 779.6 0 1066 275 634 779.6 0 634 276 958 779.6 0 958 277 887 779.6 0 887 278 1075 779.6 0 1075 279 851 779.6 0 851 280 556 779.6 0 556 281 393 779.6 0 393 282 558 779.6 0 558 283 628 779.6 0 628 284 622 779.6 0 622 285 1067 779.6 0 1067 286 807 779.6 0 807 287 753 779.6 0 753 288 787 779.6 0 787 289 669 779.6 0 669 290 966 779.6 0 966 291 828 779.6 0 828 292 1503 779.6 0 1503 293 2817 779.6 0 2817 294 3924 779.6 0 3924 295 1303 779.6 0 1303 296 1017 779.6 0 1017 297 1322 779.6 0 1322 298 1259 779.6 0 1259 299 662 779.6 0 662 300 552 779.6 0 552 301 1014 779.6 0 1014 Finished in 4.61 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 97,517 Reads with adapters: 21,583 (22.1%) Reads that were too short: 30 (0.0%) Reads written (passing filters): 21,553 (22.1%) Total basepairs processed: 20,089,130 bp Total written (filtered): 5,880,572 bp (29.3%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 21583 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 12 0.0 3 0 0 9 2 1 19 186 0.0 3 0 177 5 3 1 20 21050 0.0 4 20632 358 38 14 8 21 47 0.0 4 10 37 22 5 0.0 4 1 0 3 0 1 23 18 0.0 4 0 0 0 15 3 25 1 0.0 4 0 0 0 0 1 27 1 0.0 4 1 28 1 0.0 4 0 0 1 30 1 0.0 4 0 0 0 0 1 31 1 0.0 4 0 0 0 0 1 34 2 0.0 4 0 0 0 0 2 41 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 55 1 0.0 4 0 0 0 0 1 59 1 0.0 4 0 0 0 0 1 62 1 0.0 4 0 0 0 0 1 77 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 83 2 0.0 4 0 0 0 0 2 91 1 0.0 4 0 0 0 0 1 98 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 114 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 126 13 0.0 4 0 0 0 0 13 127 213 0.0 4 0 0 0 0 213 133 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 137 1 0.0 4 0 0 0 1 143 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 153 5 0.0 4 0 0 0 0 5 157 1 0.0 4 0 0 0 0 1 165 1 0.0 4 0 0 0 0 1 170 1 0.0 4 0 0 0 0 1 194 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.85 s (39 us/read; 1.52 M reads/minute). === Summary === Total reads processed: 21,553 Reads with adapters: 1,486 (6.9%) Reads written (passing filters): 21,553 (100.0%) Total basepairs processed: 5,880,572 bp Total written (filtered): 5,782,224 bp (98.3%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1486 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.7% C: 23.6% G: 69.1% T: 1.6% none/other: 0.1% Overview of removed sequences length count expect max.err error counts 3 7 336.8 0 7 4 1 84.2 0 1 9 1 0.1 0 1 11 1056 0.0 1 3 1053 12 31 0.0 1 0 31 98 3 0.0 1 3 152 38 0.0 1 30 8 153 1 0.0 1 1 210 8 0.0 1 7 1 224 1 0.0 1 1 225 276 0.0 1 266 10 226 10 0.0 1 10 261 49 0.0 1 45 4 262 3 0.0 1 3 264 1 0.0 1 0 1