This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CACGTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 96.16 s (30 us/read; 1.99 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 201,406 (6.3%) Reads that were too short: 157,998 (4.9%) Reads written (passing filters): 43,408 (1.4%) Total basepairs processed: 961,105,341 bp Total written (filtered): 8,497,708 bp (0.9%) === Adapter 1 === Sequence: CACGTA; Type: regular 5'; Length: 6; Trimmed: 201406 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 252 779.6 0 252 7 124 779.6 0 124 8 273 779.6 0 273 9 11404 779.6 0 11404 10 99 779.6 0 99 11 57 779.6 0 57 12 32 779.6 0 32 13 44 779.6 0 44 14 26 779.6 0 26 15 31 779.6 0 31 16 39 779.6 0 39 17 45 779.6 0 45 18 98 779.6 0 98 19 31 779.6 0 31 20 38 779.6 0 38 21 49 779.6 0 49 22 25 779.6 0 25 23 21 779.6 0 21 24 30 779.6 0 30 25 19 779.6 0 19 26 37 779.6 0 37 27 30 779.6 0 30 28 28 779.6 0 28 29 23 779.6 0 23 30 35 779.6 0 35 31 22 779.6 0 22 32 42 779.6 0 42 33 32 779.6 0 32 34 31 779.6 0 31 35 19 779.6 0 19 36 29 779.6 0 29 37 56 779.6 0 56 38 76 779.6 0 76 39 43 779.6 0 43 40 228 779.6 0 228 41 184 779.6 0 184 42 41 779.6 0 41 43 30 779.6 0 30 44 192 779.6 0 192 45 275 779.6 0 275 46 23 779.6 0 23 47 61 779.6 0 61 48 94 779.6 0 94 49 29 779.6 0 29 50 70 779.6 0 70 51 46 779.6 0 46 52 29 779.6 0 29 53 36 779.6 0 36 54 75 779.6 0 75 55 67 779.6 0 67 56 26 779.6 0 26 57 86 779.6 0 86 58 42 779.6 0 42 59 20 779.6 0 20 60 30 779.6 0 30 61 25 779.6 0 25 62 24 779.6 0 24 63 19 779.6 0 19 64 33 779.6 0 33 65 103 779.6 0 103 66 37 779.6 0 37 67 38 779.6 0 38 68 35 779.6 0 35 69 24 779.6 0 24 70 26 779.6 0 26 71 26 779.6 0 26 72 46 779.6 0 46 73 114 779.6 0 114 74 74 779.6 0 74 75 153 779.6 0 153 76 56 779.6 0 56 77 60 779.6 0 60 78 75 779.6 0 75 79 86 779.6 0 86 80 40 779.6 0 40 81 210 779.6 0 210 82 259 779.6 0 259 83 72 779.6 0 72 84 75 779.6 0 75 85 138 779.6 0 138 86 87 779.6 0 87 87 40 779.6 0 40 88 86 779.6 0 86 89 101 779.6 0 101 90 59 779.6 0 59 91 56 779.6 0 56 92 72 779.6 0 72 93 89 779.6 0 89 94 123 779.6 0 123 95 129 779.6 0 129 96 152 779.6 0 152 97 97 779.6 0 97 98 108 779.6 0 108 99 297 779.6 0 297 100 47 779.6 0 47 101 36 779.6 0 36 102 43 779.6 0 43 103 41 779.6 0 41 104 40 779.6 0 40 105 70 779.6 0 70 106 95 779.6 0 95 107 63 779.6 0 63 108 41 779.6 0 41 109 63 779.6 0 63 110 37 779.6 0 37 111 48 779.6 0 48 112 52 779.6 0 52 113 67 779.6 0 67 114 89 779.6 0 89 115 92 779.6 0 92 116 128 779.6 0 128 117 91 779.6 0 91 118 110 779.6 0 110 119 147 779.6 0 147 120 152 779.6 0 152 121 126 779.6 0 126 122 121 779.6 0 121 123 90 779.6 0 90 124 99 779.6 0 99 125 131 779.6 0 131 126 164 779.6 0 164 127 173 779.6 0 173 128 123 779.6 0 123 129 139 779.6 0 139 130 141 779.6 0 141 131 102 779.6 0 102 132 81 779.6 0 81 133 103 779.6 0 103 134 191 779.6 0 191 135 238 779.6 0 238 136 257 779.6 0 257 137 141 779.6 0 141 138 137 779.6 0 137 139 111 779.6 0 111 140 150 779.6 0 150 141 115 779.6 0 115 142 64 779.6 0 64 143 84 779.6 0 84 144 143 779.6 0 143 145 777 779.6 0 777 146 1380 779.6 0 1380 147 2227 779.6 0 2227 148 297 779.6 0 297 149 81 779.6 0 81 150 51 779.6 0 51 151 82 779.6 0 82 152 117 779.6 0 117 153 178 779.6 0 178 154 151 779.6 0 151 155 175 779.6 0 175 156 184 779.6 0 184 157 228 779.6 0 228 158 163 779.6 0 163 159 188 779.6 0 188 160 135 779.6 0 135 161 162 779.6 0 162 162 169 779.6 0 169 163 130 779.6 0 130 164 206 779.6 0 206 165 353 779.6 0 353 166 270 779.6 0 270 167 235 779.6 0 235 168 112 779.6 0 112 169 159 779.6 0 159 170 97 779.6 0 97 171 73 779.6 0 73 172 153 779.6 0 153 173 2004 779.6 0 2004 174 561 779.6 0 561 175 630 779.6 0 630 176 449 779.6 0 449 177 84 779.6 0 84 178 117 779.6 0 117 179 205 779.6 0 205 180 266 779.6 0 266 181 810 779.6 0 810 182 826 779.6 0 826 183 579 779.6 0 579 184 438 779.6 0 438 185 264 779.6 0 264 186 921 779.6 0 921 187 419 779.6 0 419 188 767 779.6 0 767 189 162 779.6 0 162 190 208 779.6 0 208 191 260 779.6 0 260 192 251 779.6 0 251 193 481 779.6 0 481 194 196 779.6 0 196 195 59 779.6 0 59 196 81 779.6 0 81 197 183 779.6 0 183 198 202 779.6 0 202 199 155 779.6 0 155 200 128 779.6 0 128 201 125 779.6 0 125 202 183 779.6 0 183 203 276 779.6 0 276 204 197 779.6 0 197 205 246 779.6 0 246 206 310 779.6 0 310 207 501 779.6 0 501 208 318 779.6 0 318 209 205 779.6 0 205 210 263 779.6 0 263 211 185 779.6 0 185 212 377 779.6 0 377 213 272 779.6 0 272 214 225 779.6 0 225 215 315 779.6 0 315 216 243 779.6 0 243 217 327 779.6 0 327 218 356 779.6 0 356 219 402 779.6 0 402 220 234 779.6 0 234 221 328 779.6 0 328 222 306 779.6 0 306 223 479 779.6 0 479 224 413 779.6 0 413 225 4286 779.6 0 4286 226 4600 779.6 0 4600 227 12948 779.6 0 12948 228 358 779.6 0 358 229 675 779.6 0 675 230 1688 779.6 0 1688 231 2491 779.6 0 2491 232 2266 779.6 0 2266 233 2512 779.6 0 2512 234 691 779.6 0 691 235 311 779.6 0 311 236 156 779.6 0 156 237 243 779.6 0 243 238 198 779.6 0 198 239 210 779.6 0 210 240 263 779.6 0 263 241 261 779.6 0 261 242 294 779.6 0 294 243 441 779.6 0 441 244 336 779.6 0 336 245 309 779.6 0 309 246 853 779.6 0 853 247 1066 779.6 0 1066 248 1299 779.6 0 1299 249 502 779.6 0 502 250 5130 779.6 0 5130 251 23357 779.6 0 23357 252 11264 779.6 0 11264 253 899 779.6 0 899 254 905 779.6 0 905 255 572 779.6 0 572 256 516 779.6 0 516 257 241 779.6 0 241 258 215 779.6 0 215 259 143 779.6 0 143 260 109 779.6 0 109 261 70 779.6 0 70 262 88 779.6 0 88 263 154 779.6 0 154 264 241 779.6 0 241 265 806 779.6 0 806 266 441 779.6 0 441 267 359 779.6 0 359 268 270 779.6 0 270 269 179 779.6 0 179 270 270 779.6 0 270 271 1002 779.6 0 1002 272 1444 779.6 0 1444 273 1433 779.6 0 1433 274 396 779.6 0 396 275 944 779.6 0 944 276 341 779.6 0 341 277 290 779.6 0 290 278 522 779.6 0 522 279 983 779.6 0 983 280 1623 779.6 0 1623 281 2277 779.6 0 2277 282 1655 779.6 0 1655 283 611 779.6 0 611 284 739 779.6 0 739 285 1474 779.6 0 1474 286 1747 779.6 0 1747 287 970 779.6 0 970 288 498 779.6 0 498 289 463 779.6 0 463 290 821 779.6 0 821 291 820 779.6 0 820 292 1096 779.6 0 1096 293 585 779.6 0 585 294 571 779.6 0 571 295 1175 779.6 0 1175 296 1276 779.6 0 1276 297 1696 779.6 0 1696 298 2681 779.6 0 2681 299 3423 779.6 0 3423 300 5401 779.6 0 5401 301 26594 779.6 0 26594 Finished in 1.90 s (44 us/read; 1.37 M reads/minute). === Summary === Total reads processed: 43,408 Reads with adapters: 11,829 (27.3%) Reads that were too short: 22 (0.1%) Reads written (passing filters): 11,807 (27.2%) Total basepairs processed: 8,497,708 bp Total written (filtered): 3,211,109 bp (37.8%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 11829 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 0 1 18 7 0.0 3 0 0 7 19 120 0.0 3 0 115 4 0 1 20 11606 0.0 4 11236 315 33 14 8 21 39 0.0 4 7 28 4 22 9 0.0 4 2 1 3 2 1 23 17 0.0 4 0 0 0 15 2 24 2 0.0 4 1 0 0 0 1 36 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 1 59 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 71 4 0.0 4 0 0 0 0 4 87 2 0.0 4 0 0 0 0 2 88 1 0.0 4 0 0 0 0 1 89 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 97 1 0.0 4 0 0 0 0 1 100 1 0.0 4 0 0 0 0 1 118 1 0.0 4 0 0 0 0 1 119 2 0.0 4 0 0 0 0 2 130 2 0.0 4 0 0 0 0 2 149 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 188 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 229 1 0.0 4 0 0 0 0 1 240 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.50 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 11,807 Reads with adapters: 1,208 (10.2%) Reads written (passing filters): 11,807 (100.0%) Total basepairs processed: 3,211,109 bp Total written (filtered): 3,198,068 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1208 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.8% C: 1.3% G: 93.5% T: 2.3% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 1 46.1 0 1 9 301 0.0 0 17 284 10 176 0.0 1 4 172 11 726 0.0 1 2 724 12 1 0.0 1 0 1 150 2 0.0 1 1 1 270 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.