This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCTGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 93.70 s (29 us/read; 2.04 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 60,750 (1.9%) Reads that were too short: 7,438 (0.2%) Reads written (passing filters): 53,312 (1.7%) Total basepairs processed: 961,105,341 bp Total written (filtered): 9,709,583 bp (1.0%) === Adapter 1 === Sequence: CCTGTC; Type: regular 5'; Length: 6; Trimmed: 60750 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 86 779.6 0 86 7 142 779.6 0 142 8 517 779.6 0 517 9 10912 779.6 0 10912 10 19 779.6 0 19 11 8 779.6 0 8 12 8 779.6 0 8 13 8 779.6 0 8 14 4 779.6 0 4 15 5 779.6 0 5 16 6 779.6 0 6 17 5 779.6 0 5 18 10 779.6 0 10 19 6 779.6 0 6 20 15 779.6 0 15 21 15 779.6 0 15 22 12 779.6 0 12 23 11 779.6 0 11 24 12 779.6 0 12 25 6 779.6 0 6 26 12 779.6 0 12 27 10 779.6 0 10 28 10 779.6 0 10 29 11 779.6 0 11 30 1023 779.6 0 1023 31 72 779.6 0 72 32 24 779.6 0 24 33 266 779.6 0 266 34 483 779.6 0 483 35 326 779.6 0 326 36 8 779.6 0 8 37 9 779.6 0 9 38 9 779.6 0 9 39 9 779.6 0 9 40 3 779.6 0 3 41 14 779.6 0 14 42 6 779.6 0 6 43 7 779.6 0 7 44 12 779.6 0 12 45 17 779.6 0 17 46 16 779.6 0 16 47 17 779.6 0 17 48 18 779.6 0 18 49 12 779.6 0 12 50 15 779.6 0 15 51 7 779.6 0 7 52 7 779.6 0 7 53 15 779.6 0 15 54 12 779.6 0 12 55 29 779.6 0 29 56 29 779.6 0 29 57 59 779.6 0 59 58 553 779.6 0 553 59 53 779.6 0 53 60 13 779.6 0 13 61 5 779.6 0 5 62 6 779.6 0 6 63 2 779.6 0 2 64 17 779.6 0 17 65 12 779.6 0 12 66 10 779.6 0 10 67 10 779.6 0 10 68 9 779.6 0 9 69 8 779.6 0 8 70 36 779.6 0 36 71 26 779.6 0 26 72 411 779.6 0 411 73 341 779.6 0 341 74 250 779.6 0 250 75 10 779.6 0 10 76 14 779.6 0 14 77 16 779.6 0 16 78 15 779.6 0 15 79 13 779.6 0 13 80 10 779.6 0 10 81 14 779.6 0 14 82 13 779.6 0 13 83 16 779.6 0 16 84 61 779.6 0 61 85 37 779.6 0 37 86 24 779.6 0 24 87 9 779.6 0 9 88 18 779.6 0 18 89 6 779.6 0 6 90 15 779.6 0 15 91 12 779.6 0 12 92 18 779.6 0 18 93 29 779.6 0 29 94 43 779.6 0 43 95 17 779.6 0 17 96 20 779.6 0 20 97 42 779.6 0 42 98 23 779.6 0 23 99 24 779.6 0 24 100 33 779.6 0 33 101 25 779.6 0 25 102 35 779.6 0 35 103 38 779.6 0 38 104 35 779.6 0 35 105 54 779.6 0 54 106 318 779.6 0 318 107 1586 779.6 0 1586 108 1705 779.6 0 1705 109 873 779.6 0 873 110 7 779.6 0 7 111 20 779.6 0 20 112 34 779.6 0 34 113 27 779.6 0 27 114 39 779.6 0 39 115 16 779.6 0 16 116 26 779.6 0 26 117 28 779.6 0 28 118 24 779.6 0 24 119 16 779.6 0 16 120 28 779.6 0 28 121 28 779.6 0 28 122 38 779.6 0 38 123 46 779.6 0 46 124 39 779.6 0 39 125 43 779.6 0 43 126 53 779.6 0 53 127 580 779.6 0 580 128 602 779.6 0 602 129 3513 779.6 0 3513 130 75 779.6 0 75 131 31 779.6 0 31 132 13 779.6 0 13 133 18 779.6 0 18 134 28 779.6 0 28 135 29 779.6 0 29 136 124 779.6 0 124 137 90 779.6 0 90 138 57 779.6 0 57 139 19 779.6 0 19 140 23 779.6 0 23 141 48 779.6 0 48 142 60 779.6 0 60 143 767 779.6 0 767 144 182 779.6 0 182 145 1700 779.6 0 1700 146 35 779.6 0 35 147 179 779.6 0 179 148 68 779.6 0 68 149 124 779.6 0 124 150 13 779.6 0 13 151 19 779.6 0 19 152 30 779.6 0 30 153 224 779.6 0 224 154 67 779.6 0 67 155 240 779.6 0 240 156 63 779.6 0 63 157 36 779.6 0 36 158 166 779.6 0 166 159 43 779.6 0 43 160 15 779.6 0 15 161 28 779.6 0 28 162 37 779.6 0 37 163 82 779.6 0 82 164 16 779.6 0 16 165 18 779.6 0 18 166 36 779.6 0 36 167 26 779.6 0 26 168 38 779.6 0 38 169 111 779.6 0 111 170 20 779.6 0 20 171 58 779.6 0 58 172 11 779.6 0 11 173 30 779.6 0 30 174 62 779.6 0 62 175 43 779.6 0 43 176 42 779.6 0 42 177 12 779.6 0 12 178 42 779.6 0 42 179 41 779.6 0 41 180 42 779.6 0 42 181 93 779.6 0 93 182 123 779.6 0 123 183 164 779.6 0 164 184 252 779.6 0 252 185 349 779.6 0 349 186 3210 779.6 0 3210 187 3240 779.6 0 3240 188 13685 779.6 0 13685 189 61 779.6 0 61 190 16 779.6 0 16 191 15 779.6 0 15 192 9 779.6 0 9 193 10 779.6 0 10 194 10 779.6 0 10 195 63 779.6 0 63 196 9 779.6 0 9 197 10 779.6 0 10 198 10 779.6 0 10 199 9 779.6 0 9 200 13 779.6 0 13 201 16 779.6 0 16 202 13 779.6 0 13 203 20 779.6 0 20 204 52 779.6 0 52 205 36 779.6 0 36 206 30 779.6 0 30 207 12 779.6 0 12 208 47 779.6 0 47 209 14 779.6 0 14 210 18 779.6 0 18 211 13 779.6 0 13 212 14 779.6 0 14 213 35 779.6 0 35 214 33 779.6 0 33 215 42 779.6 0 42 216 72 779.6 0 72 217 298 779.6 0 298 218 757 779.6 0 757 219 4268 779.6 0 4268 220 35 779.6 0 35 221 6 779.6 0 6 222 13 779.6 0 13 223 14 779.6 0 14 224 8 779.6 0 8 225 16 779.6 0 16 226 37 779.6 0 37 227 25 779.6 0 25 228 57 779.6 0 57 229 17 779.6 0 17 230 12 779.6 0 12 231 2 779.6 0 2 232 7 779.6 0 7 233 7 779.6 0 7 234 7 779.6 0 7 235 6 779.6 0 6 236 6 779.6 0 6 237 11 779.6 0 11 238 7 779.6 0 7 239 7 779.6 0 7 240 3 779.6 0 3 241 13 779.6 0 13 242 12 779.6 0 12 243 11 779.6 0 11 244 14 779.6 0 14 245 14 779.6 0 14 246 13 779.6 0 13 247 16 779.6 0 16 248 4 779.6 0 4 249 12 779.6 0 12 250 46 779.6 0 46 251 29 779.6 0 29 252 55 779.6 0 55 253 35 779.6 0 35 254 24 779.6 0 24 255 19 779.6 0 19 256 15 779.6 0 15 257 30 779.6 0 30 258 21 779.6 0 21 259 27 779.6 0 27 260 17 779.6 0 17 261 16 779.6 0 16 262 48 779.6 0 48 263 24 779.6 0 24 264 31 779.6 0 31 265 11 779.6 0 11 266 24 779.6 0 24 267 31 779.6 0 31 268 22 779.6 0 22 269 23 779.6 0 23 270 12 779.6 0 12 271 12 779.6 0 12 272 23 779.6 0 23 273 32 779.6 0 32 274 19 779.6 0 19 275 14 779.6 0 14 276 10 779.6 0 10 277 21 779.6 0 21 278 79 779.6 0 79 279 42 779.6 0 42 280 56 779.6 0 56 281 22 779.6 0 22 282 24 779.6 0 24 283 14 779.6 0 14 284 12 779.6 0 12 285 30 779.6 0 30 286 27 779.6 0 27 287 20 779.6 0 20 288 9 779.6 0 9 289 14 779.6 0 14 290 15 779.6 0 15 291 9 779.6 0 9 292 11 779.6 0 11 293 9 779.6 0 9 294 8 779.6 0 8 295 15 779.6 0 15 296 16 779.6 0 16 297 30 779.6 0 30 298 31 779.6 0 31 299 37 779.6 0 37 300 25 779.6 0 25 301 6 779.6 0 6 Finished in 2.05 s (38 us/read; 1.56 M reads/minute). === Summary === Total reads processed: 53,312 Reads with adapters: 11,590 (21.7%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 11,578 (21.7%) Total basepairs processed: 9,709,583 bp Total written (filtered): 3,180,529 bp (32.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 11590 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 3 0.0 3 0 0 3 16 94 0.0 3 0 89 4 1 17 11418 0.0 3 11170 240 6 2 18 20 0.0 3 0 18 1 1 19 8 0.0 3 0 0 5 3 20 7 0.0 3 1 0 0 6 27 1 0.0 3 0 1 31 2 0.0 3 1 0 0 1 37 1 0.0 3 0 0 0 1 43 1 0.0 3 0 0 0 1 48 8 0.0 3 0 0 0 8 51 1 0.0 3 0 0 0 1 54 1 0.0 3 0 0 0 1 56 1 0.0 3 0 0 0 1 59 2 0.0 3 0 0 0 2 62 1 0.0 3 0 0 0 1 68 1 0.0 3 0 0 0 1 74 1 0.0 3 0 0 0 1 86 1 0.0 3 0 0 0 1 109 2 0.0 3 0 0 0 2 121 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 134 4 0.0 3 0 0 0 4 137 1 0.0 3 0 0 0 1 150 2 0.0 3 0 0 0 2 153 1 0.0 3 0 0 0 1 166 3 0.0 3 0 0 0 3 181 1 0.0 3 0 0 0 1 188 1 0.0 3 0 0 0 1 263 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.47 s (41 us/read; 1.48 M reads/minute). === Summary === Total reads processed: 11,578 Reads with adapters: 1,249 (10.8%) Reads written (passing filters): 11,578 (100.0%) Total basepairs processed: 3,180,529 bp Total written (filtered): 3,176,779 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1249 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 26.3% C: 11.8% G: 61.6% T: 0.2% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1246 180.9 0 1246 4 3 45.2 0 3