This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGCAA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 97.37 s (30 us/read; 1.97 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 227,272 (7.1%) Reads that were too short: 71,832 (2.2%) Reads written (passing filters): 155,440 (4.9%) Total basepairs processed: 961,105,341 bp Total written (filtered): 44,385,951 bp (4.6%) === Adapter 1 === Sequence: TTGCAA; Type: regular 5'; Length: 6; Trimmed: 227272 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1404 779.6 0 1404 7 3108 779.6 0 3108 8 141158 779.6 0 141158 9 182 779.6 0 182 10 18 779.6 0 18 11 12 779.6 0 12 12 15 779.6 0 15 13 20 779.6 0 20 14 5 779.6 0 5 15 5 779.6 0 5 16 10 779.6 0 10 17 14 779.6 0 14 18 9 779.6 0 9 19 10 779.6 0 10 20 8 779.6 0 8 21 9 779.6 0 9 22 17 779.6 0 17 23 80 779.6 0 80 24 9 779.6 0 9 25 9 779.6 0 9 26 14 779.6 0 14 27 6 779.6 0 6 28 12 779.6 0 12 29 10 779.6 0 10 30 9 779.6 0 9 31 42 779.6 0 42 32 13 779.6 0 13 33 13 779.6 0 13 34 14 779.6 0 14 35 8 779.6 0 8 36 14 779.6 0 14 37 10 779.6 0 10 38 22 779.6 0 22 39 22 779.6 0 22 40 30 779.6 0 30 41 90 779.6 0 90 42 24 779.6 0 24 43 27 779.6 0 27 44 10 779.6 0 10 45 15 779.6 0 15 46 11 779.6 0 11 47 11 779.6 0 11 48 3 779.6 0 3 49 12 779.6 0 12 50 17 779.6 0 17 51 19 779.6 0 19 52 58 779.6 0 58 53 10 779.6 0 10 54 14 779.6 0 14 55 13 779.6 0 13 56 8 779.6 0 8 57 18 779.6 0 18 58 14 779.6 0 14 59 14 779.6 0 14 60 14 779.6 0 14 61 30 779.6 0 30 62 115 779.6 0 115 63 15 779.6 0 15 64 19 779.6 0 19 65 13 779.6 0 13 66 17 779.6 0 17 67 6 779.6 0 6 68 17 779.6 0 17 69 14 779.6 0 14 70 18 779.6 0 18 71 15 779.6 0 15 72 29 779.6 0 29 73 10 779.6 0 10 74 18 779.6 0 18 75 11 779.6 0 11 76 18 779.6 0 18 77 18 779.6 0 18 78 18 779.6 0 18 79 23 779.6 0 23 80 30 779.6 0 30 81 43 779.6 0 43 82 72 779.6 0 72 83 36 779.6 0 36 84 42 779.6 0 42 85 122 779.6 0 122 86 220 779.6 0 220 87 32 779.6 0 32 88 15 779.6 0 15 89 33 779.6 0 33 90 22 779.6 0 22 91 38 779.6 0 38 92 42 779.6 0 42 93 27 779.6 0 27 94 32 779.6 0 32 95 53 779.6 0 53 96 54 779.6 0 54 97 35 779.6 0 35 98 183 779.6 0 183 99 170 779.6 0 170 100 238 779.6 0 238 101 62 779.6 0 62 102 60 779.6 0 60 103 59 779.6 0 59 104 42 779.6 0 42 105 33 779.6 0 33 106 34 779.6 0 34 107 31 779.6 0 31 108 19 779.6 0 19 109 18 779.6 0 18 110 41 779.6 0 41 111 132 779.6 0 132 112 64 779.6 0 64 113 236 779.6 0 236 114 41 779.6 0 41 115 60 779.6 0 60 116 108 779.6 0 108 117 30 779.6 0 30 118 37 779.6 0 37 119 48 779.6 0 48 120 45 779.6 0 45 121 58 779.6 0 58 122 29 779.6 0 29 123 86 779.6 0 86 124 56 779.6 0 56 125 76 779.6 0 76 126 35 779.6 0 35 127 39 779.6 0 39 128 27 779.6 0 27 129 29 779.6 0 29 130 17 779.6 0 17 131 39 779.6 0 39 132 81 779.6 0 81 133 73 779.6 0 73 134 35 779.6 0 35 135 53 779.6 0 53 136 40 779.6 0 40 137 50 779.6 0 50 138 95 779.6 0 95 139 108 779.6 0 108 140 85 779.6 0 85 141 83 779.6 0 83 142 48 779.6 0 48 143 161 779.6 0 161 144 162 779.6 0 162 145 124 779.6 0 124 146 66 779.6 0 66 147 61 779.6 0 61 148 81 779.6 0 81 149 63 779.6 0 63 150 41 779.6 0 41 151 39 779.6 0 39 152 32 779.6 0 32 153 46 779.6 0 46 154 40 779.6 0 40 155 37 779.6 0 37 156 32 779.6 0 32 157 29 779.6 0 29 158 61 779.6 0 61 159 57 779.6 0 57 160 79 779.6 0 79 161 53 779.6 0 53 162 45 779.6 0 45 163 45 779.6 0 45 164 58 779.6 0 58 165 32 779.6 0 32 166 44 779.6 0 44 167 66 779.6 0 66 168 52 779.6 0 52 169 118 779.6 0 118 170 254 779.6 0 254 171 97 779.6 0 97 172 62 779.6 0 62 173 135 779.6 0 135 174 27 779.6 0 27 175 46 779.6 0 46 176 40 779.6 0 40 177 36 779.6 0 36 178 53 779.6 0 53 179 49 779.6 0 49 180 77 779.6 0 77 181 48 779.6 0 48 182 48 779.6 0 48 183 79 779.6 0 79 184 129 779.6 0 129 185 119 779.6 0 119 186 66 779.6 0 66 187 226 779.6 0 226 188 176 779.6 0 176 189 96 779.6 0 96 190 32 779.6 0 32 191 39 779.6 0 39 192 27 779.6 0 27 193 54 779.6 0 54 194 97 779.6 0 97 195 54 779.6 0 54 196 38 779.6 0 38 197 35 779.6 0 35 198 71 779.6 0 71 199 142 779.6 0 142 200 79 779.6 0 79 201 37 779.6 0 37 202 25 779.6 0 25 203 28 779.6 0 28 204 70 779.6 0 70 205 76 779.6 0 76 206 106 779.6 0 106 207 76 779.6 0 76 208 47 779.6 0 47 209 72 779.6 0 72 210 80 779.6 0 80 211 172 779.6 0 172 212 133 779.6 0 133 213 125 779.6 0 125 214 618 779.6 0 618 215 2351 779.6 0 2351 216 1648 779.6 0 1648 217 109 779.6 0 109 218 86 779.6 0 86 219 124 779.6 0 124 220 77 779.6 0 77 221 59 779.6 0 59 222 81 779.6 0 81 223 1207 779.6 0 1207 224 330 779.6 0 330 225 1874 779.6 0 1874 226 54 779.6 0 54 227 64 779.6 0 64 228 76 779.6 0 76 229 80 779.6 0 80 230 232 779.6 0 232 231 59 779.6 0 59 232 19 779.6 0 19 233 21 779.6 0 21 234 40 779.6 0 40 235 99 779.6 0 99 236 74 779.6 0 74 237 61 779.6 0 61 238 161 779.6 0 161 239 154 779.6 0 154 240 127 779.6 0 127 241 158 779.6 0 158 242 39 779.6 0 39 243 54 779.6 0 54 244 42 779.6 0 42 245 105 779.6 0 105 246 107 779.6 0 107 247 111 779.6 0 111 248 54 779.6 0 54 249 34 779.6 0 34 250 39 779.6 0 39 251 18 779.6 0 18 252 29 779.6 0 29 253 90 779.6 0 90 254 100 779.6 0 100 255 129 779.6 0 129 256 261 779.6 0 261 257 150 779.6 0 150 258 134 779.6 0 134 259 274 779.6 0 274 260 166 779.6 0 166 261 163 779.6 0 163 262 79 779.6 0 79 263 241 779.6 0 241 264 717 779.6 0 717 265 1365 779.6 0 1365 266 101 779.6 0 101 267 258 779.6 0 258 268 372 779.6 0 372 269 494 779.6 0 494 270 468 779.6 0 468 271 424 779.6 0 424 272 3413 779.6 0 3413 273 11325 779.6 0 11325 274 22075 779.6 0 22075 275 199 779.6 0 199 276 65 779.6 0 65 277 116 779.6 0 116 278 322 779.6 0 322 279 263 779.6 0 263 280 772 779.6 0 772 281 106 779.6 0 106 282 77 779.6 0 77 283 102 779.6 0 102 284 266 779.6 0 266 285 460 779.6 0 460 286 465 779.6 0 465 287 79 779.6 0 79 288 54 779.6 0 54 289 63 779.6 0 63 290 81 779.6 0 81 291 61 779.6 0 61 292 94 779.6 0 94 293 177 779.6 0 177 294 142 779.6 0 142 295 206 779.6 0 206 296 179 779.6 0 179 297 141 779.6 0 141 298 208 779.6 0 208 299 725 779.6 0 725 300 1415 779.6 0 1415 301 10510 779.6 0 10510 Finished in 7.13 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 155,440 Reads with adapters: 145,545 (93.6%) Reads that were too short: 2 (0.0%) Reads written (passing filters): 145,543 (93.6%) Total basepairs processed: 44,385,951 bp Total written (filtered): 39,738,435 bp (89.5%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 145545 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 7 0.0 3 0 0 0 6 1 18 92 0.0 3 0 0 61 25 6 19 1680 0.0 3 0 1563 61 37 19 20 143041 0.0 4 137986 4441 474 93 47 21 449 0.0 4 110 330 7 2 22 73 0.0 4 5 2 30 3 33 23 182 0.0 4 3 0 0 155 24 24 10 0.0 4 0 0 0 2 8 31 1 0.0 4 1 33 1 0.0 4 1 36 4 0.0 4 0 0 0 0 4 37 1 0.0 4 1 38 1 0.0 4 1 39 1 0.0 4 0 0 0 0 1 108 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 5.96 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 145,543 Reads with adapters: 35,473 (24.4%) Reads written (passing filters): 145,543 (100.0%) Total basepairs processed: 39,738,435 bp Total written (filtered): 39,274,850 bp (98.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 35473 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.6% C: 1.6% G: 94.2% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 2274.1 0 1 4 8 568.5 0 8 6 1 35.5 0 1 8 1 2.2 0 1 9 3 0.6 0 0 3 10 92 0.1 1 1 91 11 35002 0.0 1 90 34912 12 16 0.0 1 0 16 14 3 0.0 1 0 3 53 1 0.0 1 1 73 2 0.0 1 2 76 1 0.0 1 1 93 1 0.0 1 1 151 14 0.0 1 12 2 190 2 0.0 1 0 2 195 1 0.0 1 1 208 1 0.0 1 1 209 167 0.0 1 112 55 210 11 0.0 1 10 1 211 21 0.0 1 12 9 229 2 0.0 1 2 261 2 0.0 1 2 262 117 0.0 1 111 6 263 2 0.0 1 2 264 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.