This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCGACG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 98.33 s (31 us/read; 1.95 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 162,254 (5.1%) Reads that were too short: 1,428 (0.0%) Reads written (passing filters): 160,826 (5.0%) Total basepairs processed: 961,105,341 bp Total written (filtered): 46,349,179 bp (4.8%) === Adapter 1 === Sequence: TCGACG; Type: regular 5'; Length: 6; Trimmed: 162254 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1244 779.6 0 1244 7 3521 779.6 0 3521 8 144493 779.6 0 144493 9 4118 779.6 0 4118 10 26 779.6 0 26 11 45 779.6 0 45 12 21 779.6 0 21 13 17 779.6 0 17 14 13 779.6 0 13 15 20 779.6 0 20 16 15 779.6 0 15 17 14 779.6 0 14 18 14 779.6 0 14 19 16 779.6 0 16 20 14 779.6 0 14 21 15 779.6 0 15 22 12 779.6 0 12 23 13 779.6 0 13 24 14 779.6 0 14 25 16 779.6 0 16 26 8 779.6 0 8 27 4 779.6 0 4 28 14 779.6 0 14 29 14 779.6 0 14 30 10 779.6 0 10 31 9 779.6 0 9 32 7 779.6 0 7 33 17 779.6 0 17 34 19 779.6 0 19 35 10 779.6 0 10 36 9 779.6 0 9 37 9 779.6 0 9 38 13 779.6 0 13 39 12 779.6 0 12 40 11 779.6 0 11 41 18 779.6 0 18 42 10 779.6 0 10 43 10 779.6 0 10 44 9 779.6 0 9 45 10 779.6 0 10 46 8 779.6 0 8 47 11 779.6 0 11 48 14 779.6 0 14 49 16 779.6 0 16 50 12 779.6 0 12 51 9 779.6 0 9 52 11 779.6 0 11 53 5 779.6 0 5 54 13 779.6 0 13 55 5 779.6 0 5 56 9 779.6 0 9 57 10 779.6 0 10 58 7 779.6 0 7 59 13 779.6 0 13 60 28 779.6 0 28 61 16 779.6 0 16 62 30 779.6 0 30 63 7 779.6 0 7 64 9 779.6 0 9 65 27 779.6 0 27 66 203 779.6 0 203 67 49 779.6 0 49 68 51 779.6 0 51 69 8 779.6 0 8 70 5 779.6 0 5 71 13 779.6 0 13 72 20 779.6 0 20 73 2 779.6 0 2 74 9 779.6 0 9 75 4 779.6 0 4 76 7 779.6 0 7 77 3 779.6 0 3 78 7 779.6 0 7 79 5 779.6 0 5 80 9 779.6 0 9 81 2 779.6 0 2 82 12 779.6 0 12 83 13 779.6 0 13 84 6 779.6 0 6 85 6 779.6 0 6 86 7 779.6 0 7 87 6 779.6 0 6 88 5 779.6 0 5 89 4 779.6 0 4 90 11 779.6 0 11 91 83 779.6 0 83 92 34 779.6 0 34 93 21 779.6 0 21 94 33 779.6 0 33 95 1 779.6 0 1 96 10 779.6 0 10 97 38 779.6 0 38 98 101 779.6 0 101 99 12 779.6 0 12 100 7 779.6 0 7 101 11 779.6 0 11 102 16 779.6 0 16 103 19 779.6 0 19 104 35 779.6 0 35 105 6 779.6 0 6 106 5 779.6 0 5 107 18 779.6 0 18 108 16 779.6 0 16 109 12 779.6 0 12 110 21 779.6 0 21 111 169 779.6 0 169 112 73 779.6 0 73 113 209 779.6 0 209 114 25 779.6 0 25 115 64 779.6 0 64 116 97 779.6 0 97 117 1255 779.6 0 1255 118 1092 779.6 0 1092 119 1409 779.6 0 1409 120 5 779.6 0 5 121 6 779.6 0 6 122 6 779.6 0 6 123 4 779.6 0 4 124 11 779.6 0 11 125 1 779.6 0 1 126 6 779.6 0 6 127 9 779.6 0 9 128 39 779.6 0 39 129 4 779.6 0 4 130 6 779.6 0 6 131 6 779.6 0 6 132 3 779.6 0 3 133 19 779.6 0 19 134 55 779.6 0 55 135 8 779.6 0 8 136 8 779.6 0 8 137 5 779.6 0 5 138 8 779.6 0 8 139 9 779.6 0 9 140 13 779.6 0 13 141 8 779.6 0 8 142 9 779.6 0 9 143 13 779.6 0 13 144 4 779.6 0 4 145 10 779.6 0 10 146 21 779.6 0 21 147 15 779.6 0 15 148 13 779.6 0 13 149 14 779.6 0 14 150 13 779.6 0 13 151 104 779.6 0 104 152 114 779.6 0 114 153 128 779.6 0 128 154 15 779.6 0 15 155 19 779.6 0 19 156 104 779.6 0 104 157 3 779.6 0 3 158 1 779.6 0 1 159 3 779.6 0 3 160 3 779.6 0 3 161 6 779.6 0 6 162 13 779.6 0 13 163 12 779.6 0 12 164 15 779.6 0 15 165 11 779.6 0 11 166 5 779.6 0 5 167 7 779.6 0 7 168 4 779.6 0 4 169 6 779.6 0 6 170 8 779.6 0 8 171 15 779.6 0 15 172 7 779.6 0 7 173 8 779.6 0 8 174 5 779.6 0 5 175 7 779.6 0 7 176 5 779.6 0 5 177 2 779.6 0 2 178 9 779.6 0 9 179 9 779.6 0 9 180 98 779.6 0 98 181 39 779.6 0 39 182 45 779.6 0 45 183 4 779.6 0 4 184 40 779.6 0 40 185 8 779.6 0 8 187 5 779.6 0 5 188 6 779.6 0 6 189 4 779.6 0 4 190 7 779.6 0 7 191 15 779.6 0 15 192 10 779.6 0 10 193 3 779.6 0 3 194 4 779.6 0 4 195 7 779.6 0 7 196 5 779.6 0 5 197 3 779.6 0 3 198 2 779.6 0 2 199 4 779.6 0 4 200 6 779.6 0 6 201 9 779.6 0 9 202 3 779.6 0 3 203 5 779.6 0 5 204 5 779.6 0 5 205 3 779.6 0 3 206 5 779.6 0 5 207 3 779.6 0 3 208 2 779.6 0 2 209 2 779.6 0 2 210 29 779.6 0 29 211 9 779.6 0 9 212 14 779.6 0 14 213 6 779.6 0 6 214 19 779.6 0 19 215 15 779.6 0 15 216 15 779.6 0 15 217 19 779.6 0 19 218 60 779.6 0 60 219 124 779.6 0 124 220 86 779.6 0 86 221 20 779.6 0 20 222 3 779.6 0 3 223 4 779.6 0 4 224 9 779.6 0 9 225 12 779.6 0 12 226 19 779.6 0 19 227 11 779.6 0 11 228 17 779.6 0 17 229 15 779.6 0 15 230 13 779.6 0 13 231 14 779.6 0 14 232 55 779.6 0 55 233 73 779.6 0 73 234 10 779.6 0 10 235 9 779.6 0 9 236 28 779.6 0 28 237 10 779.6 0 10 238 14 779.6 0 14 239 20 779.6 0 20 240 8 779.6 0 8 241 5 779.6 0 5 242 5 779.6 0 5 243 4 779.6 0 4 244 2 779.6 0 2 245 7 779.6 0 7 246 9 779.6 0 9 247 9 779.6 0 9 248 23 779.6 0 23 249 24 779.6 0 24 250 32 779.6 0 32 251 3 779.6 0 3 252 6 779.6 0 6 253 20 779.6 0 20 254 37 779.6 0 37 255 38 779.6 0 38 256 64 779.6 0 64 257 15 779.6 0 15 258 6 779.6 0 6 259 5 779.6 0 5 260 6 779.6 0 6 261 8 779.6 0 8 262 5 779.6 0 5 263 4 779.6 0 4 264 16 779.6 0 16 265 9 779.6 0 9 266 6 779.6 0 6 267 9 779.6 0 9 268 6 779.6 0 6 269 9 779.6 0 9 270 5 779.6 0 5 271 7 779.6 0 7 272 8 779.6 0 8 273 3 779.6 0 3 274 3 779.6 0 3 275 6 779.6 0 6 276 9 779.6 0 9 277 12 779.6 0 12 278 13 779.6 0 13 279 7 779.6 0 7 280 28 779.6 0 28 281 5 779.6 0 5 282 12 779.6 0 12 283 8 779.6 0 8 284 7 779.6 0 7 285 12 779.6 0 12 286 12 779.6 0 12 287 6 779.6 0 6 288 8 779.6 0 8 289 5 779.6 0 5 290 7 779.6 0 7 291 6 779.6 0 6 292 6 779.6 0 6 293 10 779.6 0 10 294 10 779.6 0 10 295 2 779.6 0 2 296 4 779.6 0 4 297 9 779.6 0 9 298 5 779.6 0 5 299 5 779.6 0 5 300 8 779.6 0 8 Finished in 7.16 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 160,826 Reads with adapters: 148,460 (92.3%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 148,440 (92.3%) Total basepairs processed: 46,349,179 bp Total written (filtered): 40,970,912 bp (88.4%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 148460 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 10 0.0 2 0 0 0 10 15 39 0.0 3 0 0 31 8 16 1131 0.0 3 0 1101 27 3 17 145948 0.0 3 144079 1812 49 8 18 384 0.0 3 36 309 35 4 19 67 0.0 3 2 0 46 19 20 839 0.0 3 3 0 1 835 21 1 0.0 3 0 0 0 1 27 1 0.0 3 1 28 1 0.0 3 1 29 2 0.0 3 2 35 1 0.0 3 1 36 1 0.0 3 1 40 1 0.0 3 0 0 0 1 47 1 0.0 3 1 54 2 0.0 3 0 0 0 2 56 1 0.0 3 0 0 0 1 57 3 0.0 3 0 0 0 3 62 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 98 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 127 2 0.0 3 0 0 0 2 164 1 0.0 3 0 0 0 1 166 2 0.0 3 0 0 0 2 169 12 0.0 3 0 0 0 12 171 2 0.0 3 0 0 0 2 183 1 0.0 3 0 0 0 1 186 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 6.32 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 148,440 Reads with adapters: 565 (0.4%) Reads written (passing filters): 148,440 (100.0%) Total basepairs processed: 40,970,912 bp Total written (filtered): 40,969,202 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 565 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 26.4% C: 18.8% G: 50.1% T: 4.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 550 2319.4 0 550 4 15 579.8 0 15