This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTGAGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 95.78 s (30 us/read; 2.00 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 154,216 (4.8%) Reads that were too short: 30,448 (1.0%) Reads written (passing filters): 123,768 (3.9%) Total basepairs processed: 961,105,341 bp Total written (filtered): 34,795,226 bp (3.6%) === Adapter 1 === Sequence: TTGAGT; Type: regular 5'; Length: 6; Trimmed: 154216 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 504 779.6 0 504 7 861 779.6 0 861 8 4016 779.6 0 4016 9 106641 779.6 0 106641 10 173 779.6 0 173 11 37 779.6 0 37 12 38 779.6 0 38 13 22 779.6 0 22 14 32 779.6 0 32 15 49 779.6 0 49 16 34 779.6 0 34 17 39 779.6 0 39 18 34 779.6 0 34 19 39 779.6 0 39 20 26 779.6 0 26 21 46 779.6 0 46 22 28 779.6 0 28 23 20 779.6 0 20 24 33 779.6 0 33 25 37 779.6 0 37 26 30 779.6 0 30 27 22 779.6 0 22 28 23 779.6 0 23 29 36 779.6 0 36 30 49 779.6 0 49 31 21 779.6 0 21 32 32 779.6 0 32 33 30 779.6 0 30 34 29 779.6 0 29 35 31 779.6 0 31 36 31 779.6 0 31 37 17 779.6 0 17 38 37 779.6 0 37 39 30 779.6 0 30 40 30 779.6 0 30 41 34 779.6 0 34 42 23 779.6 0 23 43 18 779.6 0 18 44 32 779.6 0 32 45 86 779.6 0 86 46 39 779.6 0 39 47 35 779.6 0 35 48 56 779.6 0 56 49 30 779.6 0 30 50 31 779.6 0 31 51 41 779.6 0 41 52 32 779.6 0 32 53 30 779.6 0 30 54 36 779.6 0 36 55 36 779.6 0 36 56 26 779.6 0 26 57 29 779.6 0 29 58 38 779.6 0 38 59 149 779.6 0 149 60 40 779.6 0 40 61 75 779.6 0 75 62 29 779.6 0 29 63 31 779.6 0 31 64 28 779.6 0 28 65 21 779.6 0 21 66 24 779.6 0 24 67 30 779.6 0 30 68 24 779.6 0 24 69 28 779.6 0 28 70 49 779.6 0 49 71 51 779.6 0 51 72 172 779.6 0 172 73 34 779.6 0 34 74 37 779.6 0 37 75 32 779.6 0 32 76 40 779.6 0 40 77 134 779.6 0 134 78 209 779.6 0 209 79 34 779.6 0 34 80 46 779.6 0 46 81 32 779.6 0 32 82 57 779.6 0 57 83 47 779.6 0 47 84 37 779.6 0 37 85 57 779.6 0 57 86 32 779.6 0 32 87 32 779.6 0 32 88 35 779.6 0 35 89 39 779.6 0 39 90 32 779.6 0 32 91 45 779.6 0 45 92 45 779.6 0 45 93 45 779.6 0 45 94 47 779.6 0 47 95 55 779.6 0 55 96 35 779.6 0 35 97 39 779.6 0 39 98 79 779.6 0 79 99 43 779.6 0 43 100 43 779.6 0 43 101 26 779.6 0 26 102 35 779.6 0 35 103 39 779.6 0 39 104 32 779.6 0 32 105 33 779.6 0 33 106 37 779.6 0 37 107 41 779.6 0 41 108 27 779.6 0 27 109 28 779.6 0 28 110 42 779.6 0 42 111 33 779.6 0 33 112 72 779.6 0 72 113 145 779.6 0 145 114 85 779.6 0 85 115 39 779.6 0 39 116 86 779.6 0 86 117 50 779.6 0 50 118 101 779.6 0 101 119 30 779.6 0 30 120 26 779.6 0 26 121 26 779.6 0 26 122 29 779.6 0 29 123 28 779.6 0 28 124 30 779.6 0 30 125 33 779.6 0 33 126 33 779.6 0 33 127 29 779.6 0 29 128 46 779.6 0 46 129 72 779.6 0 72 130 83 779.6 0 83 131 99 779.6 0 99 132 67 779.6 0 67 133 80 779.6 0 80 134 124 779.6 0 124 135 51 779.6 0 51 136 50 779.6 0 50 137 46 779.6 0 46 138 94 779.6 0 94 139 57 779.6 0 57 140 87 779.6 0 87 141 51 779.6 0 51 142 143 779.6 0 143 143 135 779.6 0 135 144 170 779.6 0 170 145 1038 779.6 0 1038 146 70 779.6 0 70 147 45 779.6 0 45 148 43 779.6 0 43 149 37 779.6 0 37 150 24 779.6 0 24 151 57 779.6 0 57 152 40 779.6 0 40 153 33 779.6 0 33 154 44 779.6 0 44 155 48 779.6 0 48 156 56 779.6 0 56 157 51 779.6 0 51 158 66 779.6 0 66 159 65 779.6 0 65 160 48 779.6 0 48 161 34 779.6 0 34 162 35 779.6 0 35 163 37 779.6 0 37 164 51 779.6 0 51 165 34 779.6 0 34 166 37 779.6 0 37 167 46 779.6 0 46 168 38 779.6 0 38 169 40 779.6 0 40 170 36 779.6 0 36 171 56 779.6 0 56 172 48 779.6 0 48 173 31 779.6 0 31 174 33 779.6 0 33 175 29 779.6 0 29 176 30 779.6 0 30 177 36 779.6 0 36 178 38 779.6 0 38 179 26 779.6 0 26 180 31 779.6 0 31 181 33 779.6 0 33 182 50 779.6 0 50 183 59 779.6 0 59 184 34 779.6 0 34 185 44 779.6 0 44 186 29 779.6 0 29 187 41 779.6 0 41 188 46 779.6 0 46 189 74 779.6 0 74 190 139 779.6 0 139 191 117 779.6 0 117 192 364 779.6 0 364 193 71 779.6 0 71 194 146 779.6 0 146 195 188 779.6 0 188 196 76 779.6 0 76 197 351 779.6 0 351 198 373 779.6 0 373 199 257 779.6 0 257 200 44 779.6 0 44 201 49 779.6 0 49 202 26 779.6 0 26 203 36 779.6 0 36 204 57 779.6 0 57 205 60 779.6 0 60 206 88 779.6 0 88 207 46 779.6 0 46 208 38 779.6 0 38 209 36 779.6 0 36 210 46 779.6 0 46 211 56 779.6 0 56 212 74 779.6 0 74 213 65 779.6 0 65 214 62 779.6 0 62 215 81 779.6 0 81 216 45 779.6 0 45 217 39 779.6 0 39 218 39 779.6 0 39 219 44 779.6 0 44 220 62 779.6 0 62 221 53 779.6 0 53 222 45 779.6 0 45 223 165 779.6 0 165 224 205 779.6 0 205 225 437 779.6 0 437 226 37 779.6 0 37 227 42 779.6 0 42 228 37 779.6 0 37 229 31 779.6 0 31 230 38 779.6 0 38 231 205 779.6 0 205 232 320 779.6 0 320 233 229 779.6 0 229 234 37 779.6 0 37 235 28 779.6 0 28 236 36 779.6 0 36 237 83 779.6 0 83 238 82 779.6 0 82 239 103 779.6 0 103 240 77 779.6 0 77 241 49 779.6 0 49 242 38 779.6 0 38 243 34 779.6 0 34 244 30 779.6 0 30 245 47 779.6 0 47 246 57 779.6 0 57 247 70 779.6 0 70 248 58 779.6 0 58 249 50 779.6 0 50 250 39 779.6 0 39 251 43 779.6 0 43 252 29 779.6 0 29 253 30 779.6 0 30 254 65 779.6 0 65 255 87 779.6 0 87 256 69 779.6 0 69 257 106 779.6 0 106 258 84 779.6 0 84 259 46 779.6 0 46 260 33 779.6 0 33 261 60 779.6 0 60 262 52 779.6 0 52 263 171 779.6 0 171 264 66 779.6 0 66 265 137 779.6 0 137 266 587 779.6 0 587 267 432 779.6 0 432 268 70 779.6 0 70 269 70 779.6 0 70 270 49 779.6 0 49 271 46 779.6 0 46 272 42 779.6 0 42 273 46 779.6 0 46 274 63 779.6 0 63 275 65 779.6 0 65 276 44 779.6 0 44 277 33 779.6 0 33 278 51 779.6 0 51 279 54 779.6 0 54 280 69 779.6 0 69 281 127 779.6 0 127 282 135 779.6 0 135 283 163 779.6 0 163 284 145 779.6 0 145 285 294 779.6 0 294 286 266 779.6 0 266 287 155 779.6 0 155 288 169 779.6 0 169 289 272 779.6 0 272 290 336 779.6 0 336 291 353 779.6 0 353 292 383 779.6 0 383 293 510 779.6 0 510 294 343 779.6 0 343 295 442 779.6 0 442 296 378 779.6 0 378 297 1391 779.6 0 1391 298 2255 779.6 0 2255 299 4942 779.6 0 4942 300 4137 779.6 0 4137 301 6691 779.6 0 6691 Finished in 5.84 s (47 us/read; 1.27 M reads/minute). === Summary === Total reads processed: 123,768 Reads with adapters: 111,756 (90.3%) Reads that were too short: 2 (0.0%) Reads written (passing filters): 111,754 (90.3%) Total basepairs processed: 34,795,226 bp Total written (filtered): 30,404,427 bp (87.4%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 111756 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 3 0.0 3 0 0 0 0 3 17 8 0.0 3 0 0 0 5 3 18 100 0.0 3 0 0 70 25 5 19 1599 0.0 3 0 1532 44 14 9 20 109602 0.0 4 106074 2932 394 130 72 21 285 0.0 4 55 218 8 3 1 22 33 0.0 4 1 0 16 5 11 23 114 0.0 4 2 0 0 90 22 24 3 0.0 4 0 0 0 1 2 25 2 0.0 4 0 0 1 0 1 37 1 0.0 4 1 39 1 0.0 4 1 43 1 0.0 4 1 96 1 0.0 4 1 109 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.50 s (40 us/read; 1.49 M reads/minute). === Summary === Total reads processed: 111,754 Reads with adapters: 6,545 (5.9%) Reads written (passing filters): 111,754 (100.0%) Total basepairs processed: 30,404,427 bp Total written (filtered): 30,298,147 bp (99.7%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 6545 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.5% C: 1.9% G: 90.4% T: 2.2% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 1746.2 0 2 4 10 436.5 0 10 5 2 109.1 0 2 6 2 27.3 0 2 8 2 1.7 0 2 9 581 0.4 0 44 537 10 2417 0.1 1 71 2346 11 3321 0.0 1 8 3313 12 8 0.0 1 0 8 13 16 0.0 1 0 16 14 1 0.0 1 0 1 42 1 0.0 1 1 72 1 0.0 1 0 1 138 2 0.0 1 2 150 9 0.0 1 7 2 152 2 0.0 1 1 1 177 1 0.0 1 1 189 1 0.0 1 0 1 208 93 0.0 1 75 18 209 7 0.0 1 5 2 210 6 0.0 1 4 2 257 50 0.0 1 47 3 258 1 0.0 1 1 260 1 0.0 1 1 262 5 0.0 1 5 263 2 0.0 1 2 267 1 0.0 1 0 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.