This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g AAGGTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 98.70 s (31 us/read; 1.94 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 507,810 (15.9%) Reads that were too short: 10,035 (0.3%) Reads written (passing filters): 497,775 (15.6%) Total basepairs processed: 961,105,341 bp Total written (filtered): 113,512,683 bp (11.8%) === Adapter 1 === Sequence: AAGGTC; Type: regular 5'; Length: 6; Trimmed: 507810 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2235 779.6 0 2235 7 10733 779.6 0 10733 8 2399 779.6 0 2399 9 107892 779.6 0 107892 10 176 779.6 0 176 11 21 779.6 0 21 12 50 779.6 0 50 13 15 779.6 0 15 14 10 779.6 0 10 15 28 779.6 0 28 16 24 779.6 0 24 17 32 779.6 0 32 18 29 779.6 0 29 19 22 779.6 0 22 20 27 779.6 0 27 21 28 779.6 0 28 22 14 779.6 0 14 23 18 779.6 0 18 24 21 779.6 0 21 25 11 779.6 0 11 26 16 779.6 0 16 27 17 779.6 0 17 28 13 779.6 0 13 29 16 779.6 0 16 30 15 779.6 0 15 31 24 779.6 0 24 32 22 779.6 0 22 33 23 779.6 0 23 34 15 779.6 0 15 35 41 779.6 0 41 36 31 779.6 0 31 37 25 779.6 0 25 38 24 779.6 0 24 39 17 779.6 0 17 40 24 779.6 0 24 41 17 779.6 0 17 42 23 779.6 0 23 43 27 779.6 0 27 44 29 779.6 0 29 45 19 779.6 0 19 46 26 779.6 0 26 47 19 779.6 0 19 48 18 779.6 0 18 49 25 779.6 0 25 50 26 779.6 0 26 51 86 779.6 0 86 52 44 779.6 0 44 53 48 779.6 0 48 54 72 779.6 0 72 55 45 779.6 0 45 56 35 779.6 0 35 57 72 779.6 0 72 58 28 779.6 0 28 59 57 779.6 0 57 60 73 779.6 0 73 61 77 779.6 0 77 62 328 779.6 0 328 63 72 779.6 0 72 64 60 779.6 0 60 65 47 779.6 0 47 66 160 779.6 0 160 67 97 779.6 0 97 68 252 779.6 0 252 69 318 779.6 0 318 70 453 779.6 0 453 71 410 779.6 0 410 72 1005 779.6 0 1005 73 2422 779.6 0 2422 74 606 779.6 0 606 75 735 779.6 0 735 76 1553 779.6 0 1553 77 938 779.6 0 938 78 746 779.6 0 746 79 1823 779.6 0 1823 80 2380 779.6 0 2380 81 2330 779.6 0 2330 82 2212 779.6 0 2212 83 3627 779.6 0 3627 84 3186 779.6 0 3186 85 4321 779.6 0 4321 86 47855 779.6 0 47855 87 188990 779.6 0 188990 88 67562 779.6 0 67562 89 291 779.6 0 291 90 29 779.6 0 29 91 10 779.6 0 10 92 12 779.6 0 12 93 24 779.6 0 24 94 57 779.6 0 57 95 18 779.6 0 18 96 11 779.6 0 11 97 20 779.6 0 20 98 13 779.6 0 13 99 44 779.6 0 44 100 85 779.6 0 85 101 34 779.6 0 34 102 15 779.6 0 15 103 17 779.6 0 17 104 14 779.6 0 14 105 115 779.6 0 115 106 13 779.6 0 13 107 15 779.6 0 15 108 11 779.6 0 11 109 23 779.6 0 23 110 30 779.6 0 30 111 23 779.6 0 23 112 14 779.6 0 14 113 19 779.6 0 19 114 22 779.6 0 22 115 20 779.6 0 20 116 27 779.6 0 27 117 35 779.6 0 35 118 37 779.6 0 37 119 48 779.6 0 48 120 44 779.6 0 44 121 27 779.6 0 27 122 50 779.6 0 50 123 273 779.6 0 273 124 564 779.6 0 564 125 1044 779.6 0 1044 126 642 779.6 0 642 127 356 779.6 0 356 128 58 779.6 0 58 129 61 779.6 0 61 130 84 779.6 0 84 131 154 779.6 0 154 132 52 779.6 0 52 133 52 779.6 0 52 134 54 779.6 0 54 135 63 779.6 0 63 136 44 779.6 0 44 137 84 779.6 0 84 138 68 779.6 0 68 139 63 779.6 0 63 140 61 779.6 0 61 141 66 779.6 0 66 142 97 779.6 0 97 143 187 779.6 0 187 144 2940 779.6 0 2940 145 2927 779.6 0 2927 146 4195 779.6 0 4195 147 60 779.6 0 60 148 24 779.6 0 24 149 24 779.6 0 24 150 14 779.6 0 14 151 17 779.6 0 17 152 14 779.6 0 14 153 17 779.6 0 17 154 14 779.6 0 14 155 38 779.6 0 38 156 35 779.6 0 35 157 30 779.6 0 30 158 36 779.6 0 36 159 37 779.6 0 37 160 28 779.6 0 28 161 40 779.6 0 40 162 23 779.6 0 23 163 27 779.6 0 27 164 66 779.6 0 66 165 103 779.6 0 103 166 1330 779.6 0 1330 167 549 779.6 0 549 168 1478 779.6 0 1478 169 35 779.6 0 35 170 56 779.6 0 56 171 87 779.6 0 87 172 204 779.6 0 204 173 57 779.6 0 57 174 70 779.6 0 70 175 81 779.6 0 81 176 43 779.6 0 43 177 45 779.6 0 45 178 101 779.6 0 101 179 98 779.6 0 98 180 105 779.6 0 105 181 91 779.6 0 91 182 97 779.6 0 97 183 175 779.6 0 175 184 300 779.6 0 300 185 5457 779.6 0 5457 186 3498 779.6 0 3498 187 7657 779.6 0 7657 188 52 779.6 0 52 189 13 779.6 0 13 190 27 779.6 0 27 191 51 779.6 0 51 192 51 779.6 0 51 193 78 779.6 0 78 194 28 779.6 0 28 195 43 779.6 0 43 196 47 779.6 0 47 197 50 779.6 0 50 198 69 779.6 0 69 199 35 779.6 0 35 200 23 779.6 0 23 201 29 779.6 0 29 202 42 779.6 0 42 203 63 779.6 0 63 204 82 779.6 0 82 205 140 779.6 0 140 206 1492 779.6 0 1492 207 1101 779.6 0 1101 208 2735 779.6 0 2735 209 59 779.6 0 59 210 152 779.6 0 152 211 361 779.6 0 361 212 172 779.6 0 172 213 193 779.6 0 193 214 34 779.6 0 34 215 17 779.6 0 17 216 22 779.6 0 22 217 22 779.6 0 22 218 15 779.6 0 15 219 18 779.6 0 18 220 21 779.6 0 21 221 11 779.6 0 11 222 24 779.6 0 24 223 51 779.6 0 51 224 25 779.6 0 25 225 28 779.6 0 28 226 39 779.6 0 39 227 40 779.6 0 40 228 66 779.6 0 66 229 10 779.6 0 10 230 7 779.6 0 7 231 13 779.6 0 13 232 12 779.6 0 12 233 11 779.6 0 11 234 26 779.6 0 26 235 19 779.6 0 19 236 17 779.6 0 17 237 13 779.6 0 13 238 11 779.6 0 11 239 10 779.6 0 10 240 15 779.6 0 15 241 146 779.6 0 146 242 337 779.6 0 337 243 410 779.6 0 410 244 25 779.6 0 25 245 13 779.6 0 13 246 21 779.6 0 21 247 12 779.6 0 12 248 13 779.6 0 13 249 14 779.6 0 14 250 12 779.6 0 12 251 22 779.6 0 22 252 50 779.6 0 50 253 39 779.6 0 39 254 16 779.6 0 16 255 32 779.6 0 32 256 20 779.6 0 20 257 15 779.6 0 15 258 13 779.6 0 13 259 88 779.6 0 88 260 64 779.6 0 64 261 56 779.6 0 56 262 57 779.6 0 57 263 62 779.6 0 62 264 40 779.6 0 40 265 21 779.6 0 21 266 21 779.6 0 21 267 15 779.6 0 15 268 13 779.6 0 13 269 9 779.6 0 9 270 8 779.6 0 8 271 19 779.6 0 19 272 19 779.6 0 19 273 38 779.6 0 38 274 21 779.6 0 21 275 69 779.6 0 69 276 34 779.6 0 34 277 21 779.6 0 21 278 39 779.6 0 39 279 35 779.6 0 35 280 15 779.6 0 15 281 12 779.6 0 12 282 16 779.6 0 16 283 8 779.6 0 8 284 18 779.6 0 18 285 18 779.6 0 18 286 13 779.6 0 13 287 8 779.6 0 8 288 11 779.6 0 11 289 11 779.6 0 11 290 15 779.6 0 15 291 49 779.6 0 49 292 37 779.6 0 37 293 9 779.6 0 9 294 11 779.6 0 11 295 10 779.6 0 10 296 27 779.6 0 27 297 36 779.6 0 36 298 37 779.6 0 37 299 86 779.6 0 86 300 125 779.6 0 125 301 315 779.6 0 315 Finished in 21.14 s (42 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 497,775 Reads with adapters: 113,341 (22.8%) Reads that were too short: 30 (0.0%) Reads written (passing filters): 113,311 (22.8%) Total basepairs processed: 113,512,683 bp Total written (filtered): 31,039,467 bp (27.3%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 113341 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 5 0.0 2 0 0 0 5 15 27 0.0 3 0 0 26 1 16 635 0.0 3 0 625 9 1 17 110173 0.0 3 107838 2278 52 5 18 295 0.0 3 34 243 15 3 19 1245 0.0 3 1190 21 26 8 20 158 0.0 3 0 5 0 153 21 3 0.0 3 0 0 0 3 22 2 0.0 3 1 0 0 1 27 1 0.0 3 1 28 1 0.0 3 1 33 2 0.0 3 2 37 1 0.0 3 1 58 1 0.0 3 0 1 62 2 0.0 3 0 0 0 2 66 3 0.0 3 0 0 0 3 68 2 0.0 3 0 0 0 2 69 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 73 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 85 1 0.0 3 0 0 0 1 104 2 0.0 3 0 0 0 2 105 397 0.0 3 0 0 0 397 106 2 0.0 3 0 0 0 2 108 345 0.0 3 0 0 3 342 109 1 0.0 3 0 0 0 1 118 2 0.0 3 0 0 0 2 131 1 0.0 3 0 0 0 1 132 1 0.0 3 0 0 0 1 153 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 159 1 0.0 3 0 1 166 3 0.0 3 0 0 0 3 168 1 0.0 3 0 0 0 1 182 1 0.0 3 0 0 0 1 205 1 0.0 3 0 0 0 1 206 10 0.0 3 0 0 0 10 207 7 0.0 3 0 0 0 7 208 1 0.0 3 0 0 0 1 282 1 0.0 3 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 4.82 s (43 us/read; 1.41 M reads/minute). === Summary === Total reads processed: 113,311 Reads with adapters: 5,561 (4.9%) Reads written (passing filters): 113,311 (100.0%) Total basepairs processed: 31,039,467 bp Total written (filtered): 31,022,768 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 5561 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 79.1% C: 2.2% G: 17.9% T: 0.8% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 5547 1770.5 0 5547 4 13 442.6 0 13 6 1 27.7 0 1