This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ATAATT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 95.94 s (30 us/read; 2.00 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 485,131 (15.2%) Reads that were too short: 428,517 (13.4%) Reads written (passing filters): 56,614 (1.8%) Total basepairs processed: 961,105,341 bp Total written (filtered): 9,901,608 bp (1.0%) === Adapter 1 === Sequence: ATAATT; Type: regular 5'; Length: 6; Trimmed: 485131 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 52 779.6 0 52 7 2215 779.6 0 2215 8 30 779.6 0 30 9 26 779.6 0 26 10 29 779.6 0 29 11 39 779.6 0 39 12 19 779.6 0 19 13 23 779.6 0 23 14 32 779.6 0 32 15 24 779.6 0 24 16 16 779.6 0 16 17 34 779.6 0 34 18 101 779.6 0 101 19 33 779.6 0 33 20 14 779.6 0 14 21 22 779.6 0 22 22 22 779.6 0 22 23 26 779.6 0 26 24 23 779.6 0 23 25 33 779.6 0 33 26 36 779.6 0 36 27 34 779.6 0 34 28 35 779.6 0 35 29 55 779.6 0 55 30 89 779.6 0 89 31 1042 779.6 0 1042 32 38 779.6 0 38 33 17 779.6 0 17 34 133 779.6 0 133 35 191 779.6 0 191 36 141 779.6 0 141 37 117 779.6 0 117 38 71 779.6 0 71 39 56 779.6 0 56 40 44 779.6 0 44 41 13 779.6 0 13 42 32 779.6 0 32 43 45 779.6 0 45 44 36 779.6 0 36 45 39 779.6 0 39 46 24 779.6 0 24 47 20 779.6 0 20 48 27 779.6 0 27 49 25 779.6 0 25 50 25 779.6 0 25 51 26 779.6 0 26 52 23 779.6 0 23 53 28 779.6 0 28 54 38 779.6 0 38 55 22 779.6 0 22 56 577 779.6 0 577 57 59 779.6 0 59 58 23 779.6 0 23 59 28 779.6 0 28 60 26 779.6 0 26 61 21 779.6 0 21 62 29 779.6 0 29 63 16 779.6 0 16 64 24 779.6 0 24 65 39 779.6 0 39 66 36 779.6 0 36 67 44 779.6 0 44 68 42 779.6 0 42 69 107 779.6 0 107 70 73 779.6 0 73 71 196 779.6 0 196 72 101 779.6 0 101 73 194 779.6 0 194 74 107 779.6 0 107 75 618 779.6 0 618 76 226 779.6 0 226 77 523 779.6 0 523 78 1451 779.6 0 1451 79 269 779.6 0 269 80 235 779.6 0 235 81 438 779.6 0 438 82 331 779.6 0 331 83 133 779.6 0 133 84 235 779.6 0 235 85 119 779.6 0 119 86 194 779.6 0 194 87 146 779.6 0 146 88 343 779.6 0 343 89 52 779.6 0 52 90 202 779.6 0 202 91 439 779.6 0 439 92 202 779.6 0 202 93 327 779.6 0 327 94 259 779.6 0 259 95 125 779.6 0 125 96 283 779.6 0 283 97 601 779.6 0 601 98 1068 779.6 0 1068 99 711 779.6 0 711 100 227 779.6 0 227 101 292 779.6 0 292 102 117 779.6 0 117 103 108 779.6 0 108 104 265 779.6 0 265 105 99 779.6 0 99 106 63 779.6 0 63 107 440 779.6 0 440 108 671 779.6 0 671 109 127 779.6 0 127 110 293 779.6 0 293 111 547 779.6 0 547 112 415 779.6 0 415 113 266 779.6 0 266 114 277 779.6 0 277 115 284 779.6 0 284 116 354 779.6 0 354 117 254 779.6 0 254 118 102 779.6 0 102 119 507 779.6 0 507 120 282 779.6 0 282 121 275 779.6 0 275 122 135 779.6 0 135 123 184 779.6 0 184 124 269 779.6 0 269 125 368 779.6 0 368 126 160 779.6 0 160 127 148 779.6 0 148 128 255 779.6 0 255 129 464 779.6 0 464 130 323 779.6 0 323 131 208 779.6 0 208 132 379 779.6 0 379 133 346 779.6 0 346 134 515 779.6 0 515 135 226 779.6 0 226 136 224 779.6 0 224 137 225 779.6 0 225 138 232 779.6 0 232 139 1001 779.6 0 1001 140 371 779.6 0 371 141 399 779.6 0 399 142 169 779.6 0 169 143 300 779.6 0 300 144 168 779.6 0 168 145 220 779.6 0 220 146 209 779.6 0 209 147 224 779.6 0 224 148 182 779.6 0 182 149 165 779.6 0 165 150 194 779.6 0 194 151 115 779.6 0 115 152 175 779.6 0 175 153 262 779.6 0 262 154 300 779.6 0 300 155 644 779.6 0 644 156 315 779.6 0 315 157 392 779.6 0 392 158 431 779.6 0 431 159 3771 779.6 0 3771 160 6151 779.6 0 6151 161 4407 779.6 0 4407 162 142 779.6 0 142 163 190 779.6 0 190 164 167 779.6 0 167 165 212 779.6 0 212 166 175 779.6 0 175 167 226 779.6 0 226 168 161 779.6 0 161 169 78 779.6 0 78 170 81 779.6 0 81 171 131 779.6 0 131 172 235 779.6 0 235 173 93 779.6 0 93 174 149 779.6 0 149 175 182 779.6 0 182 176 159 779.6 0 159 177 140 779.6 0 140 178 201 779.6 0 201 179 203 779.6 0 203 180 761 779.6 0 761 181 341 779.6 0 341 182 146 779.6 0 146 183 172 779.6 0 172 184 185 779.6 0 185 185 213 779.6 0 213 186 92 779.6 0 92 187 110 779.6 0 110 188 183 779.6 0 183 189 211 779.6 0 211 190 215 779.6 0 215 191 188 779.6 0 188 192 290 779.6 0 290 193 210 779.6 0 210 194 290 779.6 0 290 195 352 779.6 0 352 196 481 779.6 0 481 197 145 779.6 0 145 198 101 779.6 0 101 199 109 779.6 0 109 200 260 779.6 0 260 201 536 779.6 0 536 202 420 779.6 0 420 203 301 779.6 0 301 204 349 779.6 0 349 205 211 779.6 0 211 206 359 779.6 0 359 207 665 779.6 0 665 208 1297 779.6 0 1297 209 961 779.6 0 961 210 449 779.6 0 449 211 414 779.6 0 414 212 667 779.6 0 667 213 699 779.6 0 699 214 409 779.6 0 409 215 238 779.6 0 238 216 214 779.6 0 214 217 173 779.6 0 173 218 694 779.6 0 694 219 266 779.6 0 266 220 363 779.6 0 363 221 823 779.6 0 823 222 474 779.6 0 474 223 500 779.6 0 500 224 485 779.6 0 485 225 375 779.6 0 375 226 192 779.6 0 192 227 384 779.6 0 384 228 1225 779.6 0 1225 229 352 779.6 0 352 230 312 779.6 0 312 231 446 779.6 0 446 232 2307 779.6 0 2307 233 15030 779.6 0 15030 234 4490 779.6 0 4490 235 545 779.6 0 545 236 386 779.6 0 386 237 621 779.6 0 621 238 1504 779.6 0 1504 239 432 779.6 0 432 240 752 779.6 0 752 241 743 779.6 0 743 242 912 779.6 0 912 243 941 779.6 0 941 244 612 779.6 0 612 245 779 779.6 0 779 246 407 779.6 0 407 247 398 779.6 0 398 248 810 779.6 0 810 249 1264 779.6 0 1264 250 2436 779.6 0 2436 251 2296 779.6 0 2296 252 2680 779.6 0 2680 253 5274 779.6 0 5274 254 12896 779.6 0 12896 255 69362 779.6 0 69362 256 152790 779.6 0 152790 257 112662 779.6 0 112662 258 2578 779.6 0 2578 259 288 779.6 0 288 260 392 779.6 0 392 261 852 779.6 0 852 262 1085 779.6 0 1085 263 814 779.6 0 814 264 316 779.6 0 316 265 522 779.6 0 522 266 417 779.6 0 417 267 117 779.6 0 117 268 189 779.6 0 189 269 182 779.6 0 182 270 201 779.6 0 201 271 289 779.6 0 289 272 373 779.6 0 373 273 263 779.6 0 263 274 198 779.6 0 198 275 203 779.6 0 203 276 194 779.6 0 194 277 210 779.6 0 210 278 181 779.6 0 181 279 137 779.6 0 137 280 118 779.6 0 118 281 138 779.6 0 138 282 312 779.6 0 312 283 377 779.6 0 377 284 385 779.6 0 385 285 176 779.6 0 176 286 242 779.6 0 242 287 229 779.6 0 229 288 159 779.6 0 159 289 448 779.6 0 448 290 354 779.6 0 354 291 354 779.6 0 354 292 239 779.6 0 239 293 168 779.6 0 168 294 199 779.6 0 199 295 279 779.6 0 279 296 363 779.6 0 363 297 894 779.6 0 894 298 1798 779.6 0 1798 299 917 779.6 0 917 300 592 779.6 0 592 301 1729 779.6 0 1729 Finished in 2.42 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 56,614 Reads with adapters: 2,229 (3.9%) Reads that were too short: 19 (0.0%) Reads written (passing filters): 2,210 (3.9%) Total basepairs processed: 9,901,608 bp Total written (filtered): 604,747 bp (6.1%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 2229 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 19 17 0.0 3 0 16 0 1 20 2176 0.0 4 2140 27 7 1 1 21 10 0.0 4 3 7 23 1 0.0 4 0 0 0 1 24 1 0.0 4 1 28 1 0.0 4 0 0 0 0 1 32 1 0.0 4 0 0 0 0 1 36 1 0.0 4 0 0 0 0 1 39 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 76 1 0.0 4 0 0 0 0 1 79 1 0.0 4 0 0 0 0 1 81 1 0.0 4 0 0 0 1 82 1 0.0 4 0 0 0 0 1 92 1 0.0 4 0 0 0 1 115 2 0.0 4 0 0 0 0 2 118 1 0.0 4 0 0 0 0 1 124 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 0 0 0 1 136 1 0.0 4 0 0 0 0 1 146 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 151 1 0.0 4 0 0 0 0 1 158 1 0.0 4 0 0 0 0 1 176 2 0.0 4 0 0 0 0 2 204 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.09 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 2,210 Reads with adapters: 77 (3.5%) Reads written (passing filters): 2,210 (100.0%) Total basepairs processed: 604,747 bp Total written (filtered): 603,491 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 77 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.2% C: 3.9% G: 90.9% T: 0.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 34.5 0 2 11 70 0.0 1 0 70 12 2 0.0 1 0 2 152 3 0.0 1 3 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.