This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GGTAAG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 96.45 s (30 us/read; 1.99 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 77,110 (2.4%) Reads that were too short: 34,737 (1.1%) Reads written (passing filters): 42,373 (1.3%) Total basepairs processed: 961,105,341 bp Total written (filtered): 7,757,012 bp (0.8%) === Adapter 1 === Sequence: GGTAAG; Type: regular 5'; Length: 6; Trimmed: 77110 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3447 779.6 0 3447 7 142 779.6 0 142 8 257 779.6 0 257 9 8491 779.6 0 8491 10 9 779.6 0 9 11 8 779.6 0 8 12 1 779.6 0 1 13 5 779.6 0 5 14 10 779.6 0 10 15 10 779.6 0 10 16 11 779.6 0 11 17 14 779.6 0 14 18 11 779.6 0 11 19 10 779.6 0 10 20 9 779.6 0 9 21 39 779.6 0 39 22 20 779.6 0 20 23 14 779.6 0 14 24 15 779.6 0 15 25 9 779.6 0 9 26 339 779.6 0 339 27 47 779.6 0 47 28 18 779.6 0 18 29 15 779.6 0 15 30 75 779.6 0 75 31 5 779.6 0 5 32 10 779.6 0 10 33 18 779.6 0 18 34 21 779.6 0 21 35 15 779.6 0 15 36 8 779.6 0 8 37 15 779.6 0 15 38 10 779.6 0 10 39 7 779.6 0 7 40 8 779.6 0 8 41 8 779.6 0 8 42 14 779.6 0 14 43 9 779.6 0 9 44 11 779.6 0 11 45 10 779.6 0 10 46 6 779.6 0 6 47 14 779.6 0 14 48 4 779.6 0 4 49 12 779.6 0 12 50 9 779.6 0 9 51 15 779.6 0 15 52 16 779.6 0 16 53 12 779.6 0 12 54 12 779.6 0 12 55 13 779.6 0 13 56 13 779.6 0 13 57 10 779.6 0 10 58 4 779.6 0 4 59 12 779.6 0 12 60 8 779.6 0 8 61 9 779.6 0 9 62 10 779.6 0 10 63 15 779.6 0 15 64 27 779.6 0 27 65 12 779.6 0 12 66 12 779.6 0 12 67 9 779.6 0 9 68 12 779.6 0 12 69 122 779.6 0 122 70 16 779.6 0 16 71 14 779.6 0 14 72 7 779.6 0 7 73 13 779.6 0 13 74 15 779.6 0 15 75 15 779.6 0 15 76 21 779.6 0 21 77 20 779.6 0 20 78 22 779.6 0 22 79 107 779.6 0 107 80 164 779.6 0 164 81 672 779.6 0 672 82 46 779.6 0 46 83 52 779.6 0 52 84 52 779.6 0 52 85 27 779.6 0 27 86 36 779.6 0 36 87 199 779.6 0 199 88 10 779.6 0 10 89 16 779.6 0 16 90 31 779.6 0 31 91 13 779.6 0 13 92 15 779.6 0 15 93 11 779.6 0 11 94 19 779.6 0 19 95 26 779.6 0 26 96 61 779.6 0 61 97 122 779.6 0 122 98 22 779.6 0 22 99 17 779.6 0 17 100 12 779.6 0 12 101 30 779.6 0 30 102 11 779.6 0 11 103 17 779.6 0 17 104 17 779.6 0 17 105 8 779.6 0 8 106 15 779.6 0 15 107 42 779.6 0 42 108 79 779.6 0 79 109 26 779.6 0 26 110 16 779.6 0 16 111 19 779.6 0 19 112 31 779.6 0 31 113 41 779.6 0 41 114 51 779.6 0 51 115 101 779.6 0 101 116 28 779.6 0 28 117 105 779.6 0 105 118 168 779.6 0 168 119 135 779.6 0 135 120 21 779.6 0 21 121 24 779.6 0 24 122 21 779.6 0 21 123 29 779.6 0 29 124 26 779.6 0 26 125 23 779.6 0 23 126 16 779.6 0 16 127 33 779.6 0 33 128 95 779.6 0 95 129 45 779.6 0 45 130 104 779.6 0 104 131 165 779.6 0 165 132 199 779.6 0 199 133 220 779.6 0 220 134 29 779.6 0 29 135 51 779.6 0 51 136 39 779.6 0 39 137 109 779.6 0 109 138 46 779.6 0 46 139 28 779.6 0 28 140 35 779.6 0 35 141 33 779.6 0 33 142 40 779.6 0 40 143 28 779.6 0 28 144 16 779.6 0 16 145 35 779.6 0 35 146 61 779.6 0 61 147 83 779.6 0 83 148 28 779.6 0 28 149 33 779.6 0 33 150 33 779.6 0 33 151 26 779.6 0 26 152 27 779.6 0 27 153 23 779.6 0 23 154 53 779.6 0 53 155 46 779.6 0 46 156 174 779.6 0 174 157 82 779.6 0 82 158 94 779.6 0 94 159 30 779.6 0 30 160 57 779.6 0 57 161 85 779.6 0 85 162 92 779.6 0 92 163 114 779.6 0 114 164 168 779.6 0 168 165 234 779.6 0 234 166 92 779.6 0 92 167 133 779.6 0 133 168 141 779.6 0 141 169 108 779.6 0 108 170 98 779.6 0 98 171 124 779.6 0 124 172 96 779.6 0 96 173 188 779.6 0 188 174 110 779.6 0 110 175 247 779.6 0 247 176 344 779.6 0 344 177 1586 779.6 0 1586 178 3696 779.6 0 3696 179 2341 779.6 0 2341 180 7421 779.6 0 7421 181 1831 779.6 0 1831 182 1407 779.6 0 1407 183 417 779.6 0 417 184 153 779.6 0 153 185 75 779.6 0 75 186 58 779.6 0 58 187 61 779.6 0 61 188 97 779.6 0 97 189 50 779.6 0 50 190 32 779.6 0 32 191 24 779.6 0 24 192 30 779.6 0 30 193 56 779.6 0 56 194 135 779.6 0 135 195 488 779.6 0 488 196 227 779.6 0 227 197 363 779.6 0 363 198 337 779.6 0 337 199 90 779.6 0 90 200 72 779.6 0 72 201 196 779.6 0 196 202 355 779.6 0 355 203 303 779.6 0 303 204 119 779.6 0 119 205 16 779.6 0 16 206 39 779.6 0 39 207 32 779.6 0 32 208 36 779.6 0 36 209 29 779.6 0 29 210 19 779.6 0 19 211 23 779.6 0 23 212 27 779.6 0 27 213 41 779.6 0 41 214 51 779.6 0 51 215 97 779.6 0 97 216 144 779.6 0 144 217 107 779.6 0 107 218 37 779.6 0 37 219 58 779.6 0 58 220 101 779.6 0 101 221 115 779.6 0 115 222 110 779.6 0 110 223 177 779.6 0 177 224 359 779.6 0 359 225 197 779.6 0 197 226 44 779.6 0 44 227 52 779.6 0 52 228 56 779.6 0 56 229 79 779.6 0 79 230 68 779.6 0 68 231 70 779.6 0 70 232 90 779.6 0 90 233 104 779.6 0 104 234 1280 779.6 0 1280 235 1165 779.6 0 1165 236 3586 779.6 0 3586 237 72 779.6 0 72 238 67 779.6 0 67 239 807 779.6 0 807 240 367 779.6 0 367 241 573 779.6 0 573 242 79 779.6 0 79 243 98 779.6 0 98 244 33 779.6 0 33 245 50 779.6 0 50 246 65 779.6 0 65 247 55 779.6 0 55 248 85 779.6 0 85 249 75 779.6 0 75 250 47 779.6 0 47 251 44 779.6 0 44 252 51 779.6 0 51 253 93 779.6 0 93 254 50 779.6 0 50 255 80 779.6 0 80 256 80 779.6 0 80 257 109 779.6 0 109 258 306 779.6 0 306 259 530 779.6 0 530 260 1863 779.6 0 1863 261 1050 779.6 0 1050 262 902 779.6 0 902 263 389 779.6 0 389 264 597 779.6 0 597 265 723 779.6 0 723 266 625 779.6 0 625 267 526 779.6 0 526 268 48 779.6 0 48 269 77 779.6 0 77 270 42 779.6 0 42 271 18 779.6 0 18 272 15 779.6 0 15 273 20 779.6 0 20 274 66 779.6 0 66 275 157 779.6 0 157 276 74 779.6 0 74 277 59 779.6 0 59 278 74 779.6 0 74 279 46 779.6 0 46 280 62 779.6 0 62 281 98 779.6 0 98 282 60 779.6 0 60 283 45 779.6 0 45 284 40 779.6 0 40 285 75 779.6 0 75 286 126 779.6 0 126 287 187 779.6 0 187 288 310 779.6 0 310 289 241 779.6 0 241 290 380 779.6 0 380 291 2813 779.6 0 2813 292 2096 779.6 0 2096 293 4547 779.6 0 4547 294 898 779.6 0 898 295 228 779.6 0 228 296 311 779.6 0 311 297 241 779.6 0 241 298 235 779.6 0 235 299 190 779.6 0 190 300 737 779.6 0 737 301 444 779.6 0 444 Finished in 1.89 s (45 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 42,373 Reads with adapters: 8,830 (20.8%) Reads that were too short: 9 (0.0%) Reads written (passing filters): 8,821 (20.8%) Total basepairs processed: 7,757,012 bp Total written (filtered): 2,398,870 bp (30.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 8830 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 16 1 0.0 3 0 0 0 0 1 18 5 0.0 3 0 0 5 19 94 0.0 3 0 84 6 2 2 20 8685 0.0 4 8410 233 28 10 4 21 15 0.0 4 2 12 1 22 2 0.0 4 0 0 2 23 11 0.0 4 0 0 0 9 2 29 3 0.0 4 0 0 0 0 3 38 1 0.0 4 0 0 0 0 1 40 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 68 1 0.0 4 0 0 0 0 1 95 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 110 1 0.0 4 0 0 0 0 1 112 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 133 1 0.0 4 0 0 0 0 1 156 1 0.0 4 0 0 0 0 1 176 1 0.0 4 0 0 0 0 1 196 1 0.0 4 0 0 0 0 1 200 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.36 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 8,821 Reads with adapters: 450 (5.1%) Reads written (passing filters): 8,821 (100.0%) Total basepairs processed: 2,398,870 bp Total written (filtered): 2,393,176 bp (99.8%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 450 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 5.1% C: 3.8% G: 89.1% T: 2.0% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 4 2 34.5 0 2 8 1 0.1 0 1 9 70 0.0 0 2 68 10 183 0.0 1 8 175 11 186 0.0 1 0 186 18 2 0.0 1 1 1 19 1 0.0 1 0 1 223 4 0.0 1 4 225 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.