This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GTACAC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 102.32 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 122,509 (3.8%) Reads that were too short: 15,978 (0.5%) Reads written (passing filters): 106,531 (3.3%) Total basepairs processed: 961,105,341 bp Total written (filtered): 29,107,238 bp (3.0%) === Adapter 1 === Sequence: GTACAC; Type: regular 5'; Length: 6; Trimmed: 122509 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3549 779.6 0 3549 7 78184 779.6 0 78184 8 271 779.6 0 271 9 7884 779.6 0 7884 10 19 779.6 0 19 11 5 779.6 0 5 12 1 779.6 0 1 13 2 779.6 0 2 14 2 779.6 0 2 15 621 779.6 0 621 16 84 779.6 0 84 18 5 779.6 0 5 19 2 779.6 0 2 20 5 779.6 0 5 21 9 779.6 0 9 22 6 779.6 0 6 23 9 779.6 0 9 25 1 779.6 0 1 27 3 779.6 0 3 28 6 779.6 0 6 29 3 779.6 0 3 30 2 779.6 0 2 31 4 779.6 0 4 32 3 779.6 0 3 33 15 779.6 0 15 34 7 779.6 0 7 35 1 779.6 0 1 36 4 779.6 0 4 37 1 779.6 0 1 38 3 779.6 0 3 39 4 779.6 0 4 40 4 779.6 0 4 41 30 779.6 0 30 42 10 779.6 0 10 43 16 779.6 0 16 44 2 779.6 0 2 45 3 779.6 0 3 46 3 779.6 0 3 47 2 779.6 0 2 48 1 779.6 0 1 49 2 779.6 0 2 50 2 779.6 0 2 51 9 779.6 0 9 52 7 779.6 0 7 53 4 779.6 0 4 54 5 779.6 0 5 55 4 779.6 0 4 56 12 779.6 0 12 57 12 779.6 0 12 58 9 779.6 0 9 59 4 779.6 0 4 60 4 779.6 0 4 61 9 779.6 0 9 62 3 779.6 0 3 63 6 779.6 0 6 64 9 779.6 0 9 65 14 779.6 0 14 66 10 779.6 0 10 67 17 779.6 0 17 68 20 779.6 0 20 69 223 779.6 0 223 70 454 779.6 0 454 71 456 779.6 0 456 72 4 779.6 0 4 73 3 779.6 0 3 74 8 779.6 0 8 75 6 779.6 0 6 76 2 779.6 0 2 77 9 779.6 0 9 78 5 779.6 0 5 79 7 779.6 0 7 80 84 779.6 0 84 81 21 779.6 0 21 82 39 779.6 0 39 83 16 779.6 0 16 84 30 779.6 0 30 85 21 779.6 0 21 86 19 779.6 0 19 87 11 779.6 0 11 88 9 779.6 0 9 89 30 779.6 0 30 90 9 779.6 0 9 91 24 779.6 0 24 92 15 779.6 0 15 93 9 779.6 0 9 94 10 779.6 0 10 95 7 779.6 0 7 96 11 779.6 0 11 97 27 779.6 0 27 98 16 779.6 0 16 99 70 779.6 0 70 100 98 779.6 0 98 101 145 779.6 0 145 102 472 779.6 0 472 103 235 779.6 0 235 104 69 779.6 0 69 105 23 779.6 0 23 106 15 779.6 0 15 107 8 779.6 0 8 108 29 779.6 0 29 109 17 779.6 0 17 110 6 779.6 0 6 111 22 779.6 0 22 112 21 779.6 0 21 113 23 779.6 0 23 114 17 779.6 0 17 115 22 779.6 0 22 116 20 779.6 0 20 117 29 779.6 0 29 118 54 779.6 0 54 119 45 779.6 0 45 120 67 779.6 0 67 121 58 779.6 0 58 122 62 779.6 0 62 123 151 779.6 0 151 124 124 779.6 0 124 125 56 779.6 0 56 126 76 779.6 0 76 127 25 779.6 0 25 128 27 779.6 0 27 129 22 779.6 0 22 130 51 779.6 0 51 131 44 779.6 0 44 132 117 779.6 0 117 133 143 779.6 0 143 134 177 779.6 0 177 135 111 779.6 0 111 136 60 779.6 0 60 137 58 779.6 0 58 138 69 779.6 0 69 139 82 779.6 0 82 140 68 779.6 0 68 141 73 779.6 0 73 142 122 779.6 0 122 143 1933 779.6 0 1933 144 537 779.6 0 537 145 56 779.6 0 56 146 61 779.6 0 61 147 63 779.6 0 63 148 147 779.6 0 147 149 101 779.6 0 101 150 87 779.6 0 87 151 54 779.6 0 54 152 54 779.6 0 54 153 56 779.6 0 56 154 40 779.6 0 40 155 40 779.6 0 40 156 80 779.6 0 80 157 95 779.6 0 95 158 232 779.6 0 232 159 413 779.6 0 413 160 433 779.6 0 433 161 319 779.6 0 319 162 214 779.6 0 214 163 87 779.6 0 87 164 66 779.6 0 66 165 54 779.6 0 54 166 77 779.6 0 77 167 84 779.6 0 84 168 141 779.6 0 141 169 106 779.6 0 106 170 195 779.6 0 195 171 62 779.6 0 62 172 111 779.6 0 111 173 28 779.6 0 28 174 54 779.6 0 54 175 100 779.6 0 100 176 1648 779.6 0 1648 177 433 779.6 0 433 178 211 779.6 0 211 179 63 779.6 0 63 180 39 779.6 0 39 181 60 779.6 0 60 182 89 779.6 0 89 183 111 779.6 0 111 184 134 779.6 0 134 185 101 779.6 0 101 186 87 779.6 0 87 187 125 779.6 0 125 188 255 779.6 0 255 189 267 779.6 0 267 190 94 779.6 0 94 191 38 779.6 0 38 192 33 779.6 0 33 193 38 779.6 0 38 194 93 779.6 0 93 195 81 779.6 0 81 196 127 779.6 0 127 197 71 779.6 0 71 198 38 779.6 0 38 199 91 779.6 0 91 200 101 779.6 0 101 201 107 779.6 0 107 202 52 779.6 0 52 203 35 779.6 0 35 204 89 779.6 0 89 205 85 779.6 0 85 206 138 779.6 0 138 207 95 779.6 0 95 208 109 779.6 0 109 209 72 779.6 0 72 210 93 779.6 0 93 211 119 779.6 0 119 212 119 779.6 0 119 213 115 779.6 0 115 214 161 779.6 0 161 215 171 779.6 0 171 216 191 779.6 0 191 217 119 779.6 0 119 218 107 779.6 0 107 219 98 779.6 0 98 220 146 779.6 0 146 221 115 779.6 0 115 222 128 779.6 0 128 223 110 779.6 0 110 224 150 779.6 0 150 225 250 779.6 0 250 226 191 779.6 0 191 227 124 779.6 0 124 228 124 779.6 0 124 229 124 779.6 0 124 230 134 779.6 0 134 231 124 779.6 0 124 232 97 779.6 0 97 233 87 779.6 0 87 234 70 779.6 0 70 235 82 779.6 0 82 236 97 779.6 0 97 237 135 779.6 0 135 238 468 779.6 0 468 239 504 779.6 0 504 240 960 779.6 0 960 241 172 779.6 0 172 242 79 779.6 0 79 243 87 779.6 0 87 244 102 779.6 0 102 245 149 779.6 0 149 246 180 779.6 0 180 247 175 779.6 0 175 248 207 779.6 0 207 249 265 779.6 0 265 250 110 779.6 0 110 251 109 779.6 0 109 252 57 779.6 0 57 253 154 779.6 0 154 254 260 779.6 0 260 255 371 779.6 0 371 256 168 779.6 0 168 257 115 779.6 0 115 258 105 779.6 0 105 259 167 779.6 0 167 260 82 779.6 0 82 261 66 779.6 0 66 262 165 779.6 0 165 263 152 779.6 0 152 264 271 779.6 0 271 265 270 779.6 0 270 266 130 779.6 0 130 267 169 779.6 0 169 268 148 779.6 0 148 269 192 779.6 0 192 270 178 779.6 0 178 271 95 779.6 0 95 272 102 779.6 0 102 273 135 779.6 0 135 274 151 779.6 0 151 275 294 779.6 0 294 276 402 779.6 0 402 277 220 779.6 0 220 278 211 779.6 0 211 279 98 779.6 0 98 280 229 779.6 0 229 281 181 779.6 0 181 282 348 779.6 0 348 283 233 779.6 0 233 284 171 779.6 0 171 285 166 779.6 0 166 286 121 779.6 0 121 287 158 779.6 0 158 288 149 779.6 0 149 289 169 779.6 0 169 290 100 779.6 0 100 291 78 779.6 0 78 292 75 779.6 0 75 293 110 779.6 0 110 294 143 779.6 0 143 295 170 779.6 0 170 296 114 779.6 0 114 297 72 779.6 0 72 298 84 779.6 0 84 299 110 779.6 0 110 300 107 779.6 0 107 301 139 779.6 0 139 Finished in 5.51 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 106,531 Reads with adapters: 8,334 (7.8%) Reads that were too short: 16 (0.0%) Reads written (passing filters): 8,318 (7.8%) Total basepairs processed: 29,107,238 bp Total written (filtered): 2,279,063 bp (7.8%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 8334 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 2 0.0 3 0 0 2 16 84 0.0 3 0 81 2 1 17 7944 0.0 3 7735 205 4 18 18 0.0 3 0 17 1 19 8 0.0 3 1 4 3 20 208 0.0 3 200 3 0 5 23 1 0.0 3 0 0 0 1 37 2 0.0 3 0 0 0 2 41 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 10 0.0 3 0 0 0 10 52 22 0.0 3 0 0 0 22 55 1 0.0 3 0 0 0 1 69 2 0.0 3 0 0 0 2 71 1 0.0 3 0 0 0 1 72 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 91 2 0.0 3 0 0 0 2 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 136 1 0.0 3 0 0 0 1 141 1 0.0 3 0 0 0 1 164 6 0.0 3 0 0 0 6 192 3 0.0 3 0 0 0 3 217 1 0.0 3 0 0 0 1 226 2 0.0 3 0 0 0 2 281 4 0.0 3 0 0 0 4 282 2 0.0 3 0 0 0 2 283 2 0.0 3 0 0 0 2 284 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.38 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 8,318 Reads with adapters: 615 (7.4%) Reads written (passing filters): 8,318 (100.0%) Total basepairs processed: 2,279,063 bp Total written (filtered): 2,277,215 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 615 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 71.4% C: 4.4% G: 22.6% T: 1.6% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 612 130.0 0 612 4 3 32.5 0 3