This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CGTGCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 98.55 s (31 us/read; 1.94 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 176,972 (5.5%) Reads that were too short: 89,024 (2.8%) Reads written (passing filters): 87,948 (2.8%) Total basepairs processed: 961,105,341 bp Total written (filtered): 23,995,209 bp (2.5%) === Adapter 1 === Sequence: CGTGCG; Type: regular 5'; Length: 6; Trimmed: 176972 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 2035 779.6 0 2035 7 1613 779.6 0 1613 8 59809 779.6 0 59809 9 63 779.6 0 63 10 48 779.6 0 48 11 15 779.6 0 15 12 11 779.6 0 11 13 18 779.6 0 18 14 16 779.6 0 16 15 18 779.6 0 18 16 26 779.6 0 26 17 23 779.6 0 23 18 15 779.6 0 15 19 45 779.6 0 45 20 82 779.6 0 82 21 26 779.6 0 26 22 5 779.6 0 5 23 7 779.6 0 7 24 16 779.6 0 16 25 14 779.6 0 14 26 8 779.6 0 8 27 9 779.6 0 9 28 23 779.6 0 23 29 16 779.6 0 16 30 34 779.6 0 34 31 12 779.6 0 12 32 16 779.6 0 16 33 23 779.6 0 23 34 14 779.6 0 14 35 14 779.6 0 14 36 11 779.6 0 11 37 14 779.6 0 14 38 10 779.6 0 10 39 18 779.6 0 18 40 17 779.6 0 17 41 6 779.6 0 6 42 18 779.6 0 18 43 18 779.6 0 18 44 11 779.6 0 11 45 7 779.6 0 7 46 20 779.6 0 20 47 15 779.6 0 15 48 29 779.6 0 29 49 24 779.6 0 24 50 16 779.6 0 16 51 42 779.6 0 42 52 17 779.6 0 17 53 21 779.6 0 21 54 32 779.6 0 32 55 38 779.6 0 38 56 39 779.6 0 39 57 28 779.6 0 28 58 47 779.6 0 47 59 32 779.6 0 32 60 69 779.6 0 69 61 163 779.6 0 163 62 278 779.6 0 278 63 59 779.6 0 59 64 88 779.6 0 88 65 159 779.6 0 159 66 46 779.6 0 46 67 41 779.6 0 41 68 71 779.6 0 71 69 51 779.6 0 51 70 130 779.6 0 130 71 116 779.6 0 116 72 143 779.6 0 143 73 165 779.6 0 165 74 301 779.6 0 301 75 4527 779.6 0 4527 76 5662 779.6 0 5662 77 9066 779.6 0 9066 78 50 779.6 0 50 79 10 779.6 0 10 80 14 779.6 0 14 81 16 779.6 0 16 82 10 779.6 0 10 83 12 779.6 0 12 84 15 779.6 0 15 85 6 779.6 0 6 86 7 779.6 0 7 87 9 779.6 0 9 88 7 779.6 0 7 89 7 779.6 0 7 90 4 779.6 0 4 91 5 779.6 0 5 92 8 779.6 0 8 93 11 779.6 0 11 94 6 779.6 0 6 95 12 779.6 0 12 96 5 779.6 0 5 97 8 779.6 0 8 98 10 779.6 0 10 99 1 779.6 0 1 100 7 779.6 0 7 101 9 779.6 0 9 102 3 779.6 0 3 103 5 779.6 0 5 104 8 779.6 0 8 105 5 779.6 0 5 106 4 779.6 0 4 107 6 779.6 0 6 108 4 779.6 0 4 109 8 779.6 0 8 110 3 779.6 0 3 111 7 779.6 0 7 112 8 779.6 0 8 113 9 779.6 0 9 114 4 779.6 0 4 115 11 779.6 0 11 116 6 779.6 0 6 117 12 779.6 0 12 118 8 779.6 0 8 119 9 779.6 0 9 120 6 779.6 0 6 121 17 779.6 0 17 122 6 779.6 0 6 123 9 779.6 0 9 124 15 779.6 0 15 125 41 779.6 0 41 126 10 779.6 0 10 127 62 779.6 0 62 128 16 779.6 0 16 129 11 779.6 0 11 130 12 779.6 0 12 131 13 779.6 0 13 132 11 779.6 0 11 133 7 779.6 0 7 134 23 779.6 0 23 135 35 779.6 0 35 136 29 779.6 0 29 137 44 779.6 0 44 138 36 779.6 0 36 139 9 779.6 0 9 140 21 779.6 0 21 141 11 779.6 0 11 142 8 779.6 0 8 143 14 779.6 0 14 144 10 779.6 0 10 145 50 779.6 0 50 146 155 779.6 0 155 147 11 779.6 0 11 148 5 779.6 0 5 149 3 779.6 0 3 150 5 779.6 0 5 151 3 779.6 0 3 152 3 779.6 0 3 153 5 779.6 0 5 154 2 779.6 0 2 155 4 779.6 0 4 156 18 779.6 0 18 157 10 779.6 0 10 158 7 779.6 0 7 159 5 779.6 0 5 160 7 779.6 0 7 161 13 779.6 0 13 162 12 779.6 0 12 163 21 779.6 0 21 164 8 779.6 0 8 165 21 779.6 0 21 166 28 779.6 0 28 167 23 779.6 0 23 168 34 779.6 0 34 169 11 779.6 0 11 170 10 779.6 0 10 171 10 779.6 0 10 172 19 779.6 0 19 173 4 779.6 0 4 174 2 779.6 0 2 175 12 779.6 0 12 176 8 779.6 0 8 177 8 779.6 0 8 178 6 779.6 0 6 179 6 779.6 0 6 180 13 779.6 0 13 181 15 779.6 0 15 182 19 779.6 0 19 183 21 779.6 0 21 184 18 779.6 0 18 185 17 779.6 0 17 186 16 779.6 0 16 187 31 779.6 0 31 188 34 779.6 0 34 189 43 779.6 0 43 190 14 779.6 0 14 191 19 779.6 0 19 192 23 779.6 0 23 193 200 779.6 0 200 194 63 779.6 0 63 195 46 779.6 0 46 196 19 779.6 0 19 197 49 779.6 0 49 198 65 779.6 0 65 199 54 779.6 0 54 200 19 779.6 0 19 201 17 779.6 0 17 202 13 779.6 0 13 203 38 779.6 0 38 204 145 779.6 0 145 205 125 779.6 0 125 206 13 779.6 0 13 207 14 779.6 0 14 208 14 779.6 0 14 209 16 779.6 0 16 210 36 779.6 0 36 211 55 779.6 0 55 212 57 779.6 0 57 213 31 779.6 0 31 214 38 779.6 0 38 215 55 779.6 0 55 216 38 779.6 0 38 217 41 779.6 0 41 218 65 779.6 0 65 219 108 779.6 0 108 220 44 779.6 0 44 221 68 779.6 0 68 222 71 779.6 0 71 223 444 779.6 0 444 224 583 779.6 0 583 225 943 779.6 0 943 226 70 779.6 0 70 227 64 779.6 0 64 228 42 779.6 0 42 229 87 779.6 0 87 230 70 779.6 0 70 231 101 779.6 0 101 232 110 779.6 0 110 233 161 779.6 0 161 234 341 779.6 0 341 235 700 779.6 0 700 236 562 779.6 0 562 237 461 779.6 0 461 238 4597 779.6 0 4597 239 2866 779.6 0 2866 240 9021 779.6 0 9021 241 307 779.6 0 307 242 916 779.6 0 916 243 383 779.6 0 383 244 375 779.6 0 375 245 520 779.6 0 520 246 575 779.6 0 575 247 844 779.6 0 844 248 6778 779.6 0 6778 249 24807 779.6 0 24807 250 21862 779.6 0 21862 251 3846 779.6 0 3846 252 57 779.6 0 57 253 57 779.6 0 57 254 58 779.6 0 58 255 16 779.6 0 16 256 17 779.6 0 17 257 17 779.6 0 17 258 6 779.6 0 6 259 16 779.6 0 16 260 138 779.6 0 138 261 6 779.6 0 6 262 37 779.6 0 37 263 7 779.6 0 7 264 29 779.6 0 29 265 8 779.6 0 8 266 19 779.6 0 19 267 20 779.6 0 20 268 23 779.6 0 23 269 31 779.6 0 31 270 16 779.6 0 16 271 11 779.6 0 11 272 16 779.6 0 16 273 14 779.6 0 14 274 8 779.6 0 8 275 12 779.6 0 12 276 26 779.6 0 26 277 20 779.6 0 20 278 46 779.6 0 46 279 122 779.6 0 122 280 236 779.6 0 236 281 290 779.6 0 290 282 1257 779.6 0 1257 283 502 779.6 0 502 284 1107 779.6 0 1107 285 40 779.6 0 40 286 35 779.6 0 35 287 25 779.6 0 25 288 45 779.6 0 45 289 31 779.6 0 31 290 11 779.6 0 11 291 12 779.6 0 12 292 9 779.6 0 9 293 16 779.6 0 16 294 65 779.6 0 65 295 178 779.6 0 178 296 257 779.6 0 257 297 261 779.6 0 261 298 21 779.6 0 21 299 34 779.6 0 34 300 107 779.6 0 107 301 111 779.6 0 111 Finished in 4.39 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 87,948 Reads with adapters: 63,203 (71.9%) Reads that were too short: 20 (0.0%) Reads written (passing filters): 63,183 (71.8%) Total basepairs processed: 23,995,209 bp Total written (filtered): 17,252,023 bp (71.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 63203 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 0 0 0 2 3 18 50 0.0 3 0 0 42 7 1 19 944 0.0 3 0 875 34 25 10 20 61201 0.0 4 59017 1903 210 52 19 21 168 0.0 4 29 131 5 3 22 732 0.0 4 691 26 8 3 4 23 67 0.0 4 1 3 1 53 9 24 1 0.0 4 0 0 0 0 1 25 1 0.0 4 0 0 1 36 1 0.0 4 0 1 37 2 0.0 4 2 59 11 0.0 4 0 0 0 0 11 85 1 0.0 4 0 0 0 0 1 125 1 0.0 4 0 0 0 0 1 139 1 0.0 4 0 0 0 0 1 140 1 0.0 4 0 0 0 0 1 148 1 0.0 4 0 0 0 0 1 157 1 0.0 4 0 0 0 0 1 160 2 0.0 4 0 0 0 0 2 165 1 0.0 4 0 0 0 0 1 177 5 0.0 4 0 0 0 0 5 186 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 190 1 0.0 4 0 0 0 0 1 207 1 0.0 4 0 0 0 0 1 214 1 0.0 4 0 0 0 0 1 217 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.58 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 63,183 Reads with adapters: 14,098 (22.3%) Reads written (passing filters): 63,183 (100.0%) Total basepairs processed: 17,252,023 bp Total written (filtered): 16,999,642 bp (98.5%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 14098 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 4.1% C: 1.4% G: 92.7% T: 1.8% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 2 987.2 0 2 4 1 246.8 0 1 5 2 61.7 0 2 8 1 1.0 0 1 9 3 0.2 0 0 3 10 290 0.1 1 12 278 11 13380 0.0 1 28 13352 12 19 0.0 1 1 18 13 2 0.0 1 0 2 31 1 0.0 1 1 151 3 0.0 1 2 1 153 1 0.0 1 0 1 209 1 0.0 1 0 1 224 7 0.0 1 7 225 1 0.0 1 1 231 5 0.0 1 5 258 8 0.0 1 7 1 259 353 0.0 1 340 13 260 11 0.0 1 10 1 261 5 0.0 1 5 269 1 0.0 1 1 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.