This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GCGAGA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 98.62 s (31 us/read; 1.94 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 52,571 (1.6%) Reads that were too short: 11,400 (0.4%) Reads written (passing filters): 41,171 (1.3%) Total basepairs processed: 961,105,341 bp Total written (filtered): 9,384,958 bp (1.0%) === Adapter 1 === Sequence: GCGAGA; Type: regular 5'; Length: 6; Trimmed: 52571 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 475 779.6 0 475 7 13687 779.6 0 13687 8 5557 779.6 0 5557 9 13 779.6 0 13 10 12 779.6 0 12 11 53 779.6 0 53 12 11 779.6 0 11 13 11 779.6 0 11 14 10 779.6 0 10 15 5 779.6 0 5 16 8 779.6 0 8 17 9 779.6 0 9 18 8 779.6 0 8 19 5 779.6 0 5 20 6 779.6 0 6 21 7 779.6 0 7 22 9 779.6 0 9 23 8 779.6 0 8 24 9 779.6 0 9 25 12 779.6 0 12 26 7 779.6 0 7 27 9 779.6 0 9 28 16 779.6 0 16 29 12 779.6 0 12 30 56 779.6 0 56 31 90 779.6 0 90 32 29 779.6 0 29 33 16 779.6 0 16 34 28 779.6 0 28 35 20 779.6 0 20 36 27 779.6 0 27 37 47 779.6 0 47 38 25 779.6 0 25 39 30 779.6 0 30 40 23 779.6 0 23 41 22 779.6 0 22 42 19 779.6 0 19 43 32 779.6 0 32 44 25 779.6 0 25 45 26 779.6 0 26 46 23 779.6 0 23 47 42 779.6 0 42 48 38 779.6 0 38 49 53 779.6 0 53 50 824 779.6 0 824 51 1055 779.6 0 1055 52 1733 779.6 0 1733 53 24 779.6 0 24 54 10 779.6 0 10 55 12 779.6 0 12 56 53 779.6 0 53 57 17 779.6 0 17 58 14 779.6 0 14 59 15 779.6 0 15 60 29 779.6 0 29 61 25 779.6 0 25 62 11 779.6 0 11 63 26 779.6 0 26 64 20 779.6 0 20 65 56 779.6 0 56 66 17 779.6 0 17 67 15 779.6 0 15 68 22 779.6 0 22 69 35 779.6 0 35 70 35 779.6 0 35 71 16 779.6 0 16 72 20 779.6 0 20 73 36 779.6 0 36 74 192 779.6 0 192 75 1317 779.6 0 1317 76 490 779.6 0 490 77 13 779.6 0 13 78 19 779.6 0 19 79 11 779.6 0 11 80 18 779.6 0 18 81 15 779.6 0 15 82 14 779.6 0 14 83 8 779.6 0 8 84 10 779.6 0 10 85 11 779.6 0 11 86 12 779.6 0 12 87 11 779.6 0 11 88 11 779.6 0 11 89 7 779.6 0 7 90 13 779.6 0 13 91 9 779.6 0 9 92 13 779.6 0 13 93 11 779.6 0 11 94 7 779.6 0 7 95 10 779.6 0 10 96 17 779.6 0 17 97 5 779.6 0 5 98 13 779.6 0 13 99 12 779.6 0 12 100 12 779.6 0 12 101 14 779.6 0 14 102 20 779.6 0 20 103 17 779.6 0 17 104 17 779.6 0 17 105 26 779.6 0 26 106 17 779.6 0 17 107 8 779.6 0 8 108 4 779.6 0 4 109 13 779.6 0 13 110 14 779.6 0 14 111 20 779.6 0 20 112 22 779.6 0 22 113 13 779.6 0 13 114 506 779.6 0 506 115 85 779.6 0 85 116 56 779.6 0 56 117 20 779.6 0 20 118 33 779.6 0 33 119 36 779.6 0 36 120 24 779.6 0 24 121 29 779.6 0 29 122 21 779.6 0 21 123 31 779.6 0 31 124 51 779.6 0 51 125 328 779.6 0 328 126 197 779.6 0 197 127 711 779.6 0 711 128 14 779.6 0 14 129 26 779.6 0 26 130 100 779.6 0 100 131 35 779.6 0 35 132 46 779.6 0 46 133 24 779.6 0 24 134 31 779.6 0 31 135 222 779.6 0 222 136 193 779.6 0 193 137 98 779.6 0 98 138 36 779.6 0 36 139 24 779.6 0 24 140 31 779.6 0 31 141 17 779.6 0 17 142 30 779.6 0 30 143 14 779.6 0 14 144 65 779.6 0 65 145 25 779.6 0 25 146 136 779.6 0 136 147 30 779.6 0 30 148 21 779.6 0 21 149 22 779.6 0 22 150 40 779.6 0 40 151 27 779.6 0 27 152 8 779.6 0 8 153 13 779.6 0 13 154 24 779.6 0 24 155 16 779.6 0 16 156 21 779.6 0 21 157 55 779.6 0 55 158 25 779.6 0 25 159 33 779.6 0 33 160 32 779.6 0 32 161 29 779.6 0 29 162 45 779.6 0 45 163 43 779.6 0 43 164 916 779.6 0 916 165 670 779.6 0 670 166 376 779.6 0 376 167 28 779.6 0 28 168 16 779.6 0 16 169 40 779.6 0 40 170 39 779.6 0 39 171 27 779.6 0 27 172 13 779.6 0 13 173 25 779.6 0 25 174 14 779.6 0 14 175 30 779.6 0 30 176 32 779.6 0 32 177 16 779.6 0 16 178 17 779.6 0 17 179 17 779.6 0 17 180 19 779.6 0 19 181 34 779.6 0 34 182 35 779.6 0 35 183 36 779.6 0 36 184 21 779.6 0 21 185 46 779.6 0 46 186 58 779.6 0 58 187 48 779.6 0 48 188 17 779.6 0 17 189 18 779.6 0 18 190 24 779.6 0 24 191 100 779.6 0 100 192 154 779.6 0 154 193 1361 779.6 0 1361 194 66 779.6 0 66 195 96 779.6 0 96 196 329 779.6 0 329 197 677 779.6 0 677 198 2931 779.6 0 2931 199 1625 779.6 0 1625 200 117 779.6 0 117 201 68 779.6 0 68 202 20 779.6 0 20 203 15 779.6 0 15 204 50 779.6 0 50 205 53 779.6 0 53 206 26 779.6 0 26 207 40 779.6 0 40 208 26 779.6 0 26 209 36 779.6 0 36 210 28 779.6 0 28 211 29 779.6 0 29 212 29 779.6 0 29 213 56 779.6 0 56 214 38 779.6 0 38 215 59 779.6 0 59 216 72 779.6 0 72 217 135 779.6 0 135 218 501 779.6 0 501 219 343 779.6 0 343 220 38 779.6 0 38 221 64 779.6 0 64 222 28 779.6 0 28 223 19 779.6 0 19 224 20 779.6 0 20 225 31 779.6 0 31 226 21 779.6 0 21 227 33 779.6 0 33 228 36 779.6 0 36 229 20 779.6 0 20 230 52 779.6 0 52 231 29 779.6 0 29 232 20 779.6 0 20 233 24 779.6 0 24 234 21 779.6 0 21 235 16 779.6 0 16 236 40 779.6 0 40 237 93 779.6 0 93 238 95 779.6 0 95 239 386 779.6 0 386 240 517 779.6 0 517 241 96 779.6 0 96 242 28 779.6 0 28 243 14 779.6 0 14 244 19 779.6 0 19 245 24 779.6 0 24 246 134 779.6 0 134 247 1304 779.6 0 1304 248 4029 779.6 0 4029 249 527 779.6 0 527 250 232 779.6 0 232 251 30 779.6 0 30 252 14 779.6 0 14 253 15 779.6 0 15 254 25 779.6 0 25 255 35 779.6 0 35 256 34 779.6 0 34 257 39 779.6 0 39 258 76 779.6 0 76 259 40 779.6 0 40 260 25 779.6 0 25 261 11 779.6 0 11 262 10 779.6 0 10 263 14 779.6 0 14 264 14 779.6 0 14 265 19 779.6 0 19 266 49 779.6 0 49 267 40 779.6 0 40 268 45 779.6 0 45 269 25 779.6 0 25 270 45 779.6 0 45 271 103 779.6 0 103 272 13 779.6 0 13 273 13 779.6 0 13 274 19 779.6 0 19 275 22 779.6 0 22 276 38 779.6 0 38 277 29 779.6 0 29 278 32 779.6 0 32 279 22 779.6 0 22 280 31 779.6 0 31 281 61 779.6 0 61 282 45 779.6 0 45 283 15 779.6 0 15 284 31 779.6 0 31 285 30 779.6 0 30 286 38 779.6 0 38 287 79 779.6 0 79 288 32 779.6 0 32 289 30 779.6 0 30 290 45 779.6 0 45 291 54 779.6 0 54 292 134 779.6 0 134 293 33 779.6 0 33 294 78 779.6 0 78 295 45 779.6 0 45 296 41 779.6 0 41 297 25 779.6 0 25 298 23 779.6 0 23 299 17 779.6 0 17 300 15 779.6 0 15 301 36 779.6 0 36 Finished in 1.83 s (44 us/read; 1.35 M reads/minute). === Summary === Total reads processed: 41,171 Reads with adapters: 14,043 (34.1%) Reads that were too short: 60 (0.1%) Reads written (passing filters): 13,983 (34.0%) Total basepairs processed: 9,384,958 bp Total written (filtered): 3,865,501 bp (41.2%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 14043 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 4 0.0 3 0 0 4 16 108 0.0 3 0 105 2 1 17 13764 0.0 3 13513 242 8 1 18 33 0.0 3 3 28 1 1 19 7 0.0 3 0 0 4 3 20 18 0.0 3 0 0 0 18 27 2 0.0 3 0 0 0 2 30 5 0.0 3 0 0 1 4 35 2 0.0 3 0 0 0 2 46 1 0.0 3 0 0 0 1 48 1 0.0 3 0 0 0 1 49 2 0.0 3 0 0 0 2 50 1 0.0 3 0 0 0 1 62 1 0.0 3 0 0 0 1 75 2 0.0 3 0 0 0 2 78 1 0.0 3 0 0 0 1 87 10 0.0 3 0 0 0 10 89 1 0.0 3 0 0 0 1 90 35 0.0 3 0 0 0 35 91 1 0.0 3 0 0 0 1 95 1 0.0 3 0 0 0 1 96 2 0.0 3 0 0 0 2 109 1 0.0 3 0 0 0 1 111 2 0.0 3 0 0 0 2 114 1 0.0 3 0 0 0 1 115 1 0.0 3 0 0 0 1 117 6 0.0 3 0 0 0 6 118 1 0.0 3 0 0 0 1 120 12 0.0 3 0 0 0 12 138 1 0.0 3 0 0 0 1 141 2 0.0 3 0 0 0 2 144 2 0.0 3 0 0 0 2 148 1 0.0 3 0 0 0 1 156 1 0.0 3 0 0 0 1 158 1 0.0 3 0 0 0 1 192 5 0.0 3 0 0 0 5 209 1 0.0 3 0 0 0 1 211 1 0.0 3 0 0 0 1 212 1 0.0 3 0 0 0 1 250 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.60 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 13,983 Reads with adapters: 598 (4.3%) Reads written (passing filters): 13,983 (100.0%) Total basepairs processed: 3,865,501 bp Total written (filtered): 3,863,707 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 598 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 5.2% C: 78.8% G: 14.7% T: 1.3% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 598 218.5 0 598