This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CCGTCC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 98.23 s (31 us/read; 1.95 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 72,536 (2.3%) Reads that were too short: 8,320 (0.3%) Reads written (passing filters): 64,216 (2.0%) Total basepairs processed: 961,105,341 bp Total written (filtered): 17,540,497 bp (1.8%) === Adapter 1 === Sequence: CCGTCC; Type: regular 5'; Length: 6; Trimmed: 72536 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 519 779.6 0 519 7 807 779.6 0 807 8 1699 779.6 0 1699 9 50542 779.6 0 50542 10 105 779.6 0 105 11 13 779.6 0 13 12 20 779.6 0 20 13 9 779.6 0 9 14 10 779.6 0 10 15 11 779.6 0 11 16 10 779.6 0 10 17 8 779.6 0 8 18 12 779.6 0 12 19 12 779.6 0 12 20 8 779.6 0 8 21 15 779.6 0 15 22 7 779.6 0 7 23 11 779.6 0 11 24 11 779.6 0 11 25 7 779.6 0 7 26 5 779.6 0 5 27 19 779.6 0 19 28 18 779.6 0 18 29 14 779.6 0 14 30 6 779.6 0 6 31 5 779.6 0 5 32 6 779.6 0 6 33 7 779.6 0 7 34 7 779.6 0 7 35 11 779.6 0 11 36 18 779.6 0 18 37 13 779.6 0 13 38 10 779.6 0 10 39 7 779.6 0 7 40 17 779.6 0 17 41 8 779.6 0 8 42 12 779.6 0 12 43 18 779.6 0 18 44 5 779.6 0 5 45 10 779.6 0 10 46 12 779.6 0 12 47 12 779.6 0 12 48 22 779.6 0 22 49 18 779.6 0 18 50 12 779.6 0 12 51 8 779.6 0 8 52 22 779.6 0 22 53 188 779.6 0 188 54 24 779.6 0 24 55 24 779.6 0 24 56 13 779.6 0 13 57 9 779.6 0 9 58 19 779.6 0 19 59 20 779.6 0 20 60 13 779.6 0 13 61 16 779.6 0 16 62 20 779.6 0 20 63 59 779.6 0 59 64 75 779.6 0 75 65 134 779.6 0 134 66 14 779.6 0 14 67 21 779.6 0 21 68 24 779.6 0 24 69 34 779.6 0 34 70 32 779.6 0 32 71 49 779.6 0 49 72 75 779.6 0 75 73 882 779.6 0 882 74 761 779.6 0 761 75 843 779.6 0 843 76 14 779.6 0 14 77 18 779.6 0 18 78 27 779.6 0 27 79 20 779.6 0 20 80 13 779.6 0 13 81 12 779.6 0 12 82 12 779.6 0 12 83 17 779.6 0 17 84 23 779.6 0 23 85 26 779.6 0 26 86 61 779.6 0 61 87 31 779.6 0 31 88 60 779.6 0 60 89 25 779.6 0 25 90 12 779.6 0 12 91 19 779.6 0 19 92 15 779.6 0 15 93 9 779.6 0 9 94 18 779.6 0 18 95 9 779.6 0 9 96 12 779.6 0 12 97 10 779.6 0 10 98 15 779.6 0 15 99 12 779.6 0 12 100 4 779.6 0 4 101 4 779.6 0 4 102 15 779.6 0 15 103 8 779.6 0 8 104 12 779.6 0 12 105 23 779.6 0 23 106 78 779.6 0 78 107 17 779.6 0 17 108 22 779.6 0 22 109 28 779.6 0 28 110 38 779.6 0 38 111 12 779.6 0 12 112 10 779.6 0 10 113 10 779.6 0 10 114 6 779.6 0 6 115 17 779.6 0 17 116 12 779.6 0 12 117 15 779.6 0 15 118 10 779.6 0 10 119 10 779.6 0 10 120 7 779.6 0 7 121 23 779.6 0 23 122 13 779.6 0 13 123 25 779.6 0 25 124 14 779.6 0 14 125 22 779.6 0 22 126 16 779.6 0 16 127 16 779.6 0 16 128 27 779.6 0 27 129 40 779.6 0 40 130 33 779.6 0 33 131 32 779.6 0 32 132 29 779.6 0 29 133 9 779.6 0 9 134 14 779.6 0 14 135 119 779.6 0 119 136 88 779.6 0 88 137 105 779.6 0 105 138 113 779.6 0 113 139 24 779.6 0 24 140 21 779.6 0 21 141 27 779.6 0 27 142 41 779.6 0 41 143 23 779.6 0 23 144 11 779.6 0 11 145 33 779.6 0 33 146 121 779.6 0 121 147 173 779.6 0 173 148 130 779.6 0 130 149 24 779.6 0 24 150 25 779.6 0 25 151 43 779.6 0 43 152 11 779.6 0 11 153 26 779.6 0 26 154 22 779.6 0 22 155 79 779.6 0 79 156 91 779.6 0 91 157 111 779.6 0 111 158 150 779.6 0 150 159 197 779.6 0 197 160 324 779.6 0 324 161 46 779.6 0 46 162 73 779.6 0 73 163 80 779.6 0 80 164 27 779.6 0 27 165 35 779.6 0 35 166 62 779.6 0 62 167 86 779.6 0 86 168 59 779.6 0 59 169 55 779.6 0 55 170 57 779.6 0 57 171 216 779.6 0 216 172 54 779.6 0 54 173 55 779.6 0 55 174 219 779.6 0 219 175 110 779.6 0 110 176 101 779.6 0 101 177 39 779.6 0 39 178 12 779.6 0 12 179 22 779.6 0 22 180 183 779.6 0 183 181 112 779.6 0 112 182 78 779.6 0 78 183 65 779.6 0 65 184 44 779.6 0 44 185 66 779.6 0 66 186 111 779.6 0 111 187 372 779.6 0 372 188 358 779.6 0 358 189 310 779.6 0 310 190 9 779.6 0 9 191 13 779.6 0 13 192 31 779.6 0 31 193 21 779.6 0 21 194 140 779.6 0 140 195 21 779.6 0 21 196 13 779.6 0 13 197 12 779.6 0 12 198 6 779.6 0 6 199 6 779.6 0 6 200 15 779.6 0 15 201 22 779.6 0 22 202 25 779.6 0 25 203 39 779.6 0 39 204 64 779.6 0 64 205 38 779.6 0 38 206 17 779.6 0 17 207 50 779.6 0 50 208 23 779.6 0 23 209 23 779.6 0 23 210 10 779.6 0 10 211 17 779.6 0 17 212 14 779.6 0 14 213 17 779.6 0 17 214 34 779.6 0 34 215 13 779.6 0 13 216 44 779.6 0 44 217 29 779.6 0 29 218 24 779.6 0 24 219 13 779.6 0 13 220 19 779.6 0 19 221 29 779.6 0 29 222 29 779.6 0 29 223 17 779.6 0 17 224 21 779.6 0 21 225 30 779.6 0 30 226 40 779.6 0 40 227 49 779.6 0 49 228 138 779.6 0 138 229 58 779.6 0 58 230 12 779.6 0 12 231 30 779.6 0 30 232 38 779.6 0 38 233 43 779.6 0 43 234 16 779.6 0 16 235 15 779.6 0 15 236 15 779.6 0 15 237 41 779.6 0 41 238 42 779.6 0 42 239 20 779.6 0 20 240 15 779.6 0 15 241 23 779.6 0 23 242 40 779.6 0 40 243 20 779.6 0 20 244 25 779.6 0 25 245 24 779.6 0 24 246 38 779.6 0 38 247 139 779.6 0 139 248 89 779.6 0 89 249 46 779.6 0 46 250 60 779.6 0 60 251 446 779.6 0 446 252 566 779.6 0 566 253 3361 779.6 0 3361 254 177 779.6 0 177 255 62 779.6 0 62 256 35 779.6 0 35 257 53 779.6 0 53 258 30 779.6 0 30 259 23 779.6 0 23 260 38 779.6 0 38 261 31 779.6 0 31 262 44 779.6 0 44 263 93 779.6 0 93 264 55 779.6 0 55 265 28 779.6 0 28 266 19 779.6 0 19 267 26 779.6 0 26 268 43 779.6 0 43 269 25 779.6 0 25 270 13 779.6 0 13 271 15 779.6 0 15 272 19 779.6 0 19 273 23 779.6 0 23 274 19 779.6 0 19 275 13 779.6 0 13 276 18 779.6 0 18 277 20 779.6 0 20 278 44 779.6 0 44 279 40 779.6 0 40 280 36 779.6 0 36 281 86 779.6 0 86 282 72 779.6 0 72 283 149 779.6 0 149 284 18 779.6 0 18 285 71 779.6 0 71 286 24 779.6 0 24 287 44 779.6 0 44 288 39 779.6 0 39 289 11 779.6 0 11 290 25 779.6 0 25 291 26 779.6 0 26 292 39 779.6 0 39 293 56 779.6 0 56 294 32 779.6 0 32 295 82 779.6 0 82 296 45 779.6 0 45 297 46 779.6 0 46 298 22 779.6 0 22 299 154 779.6 0 154 300 103 779.6 0 103 301 46 779.6 0 46 Finished in 3.14 s (49 us/read; 1.23 M reads/minute). === Summary === Total reads processed: 64,216 Reads with adapters: 53,335 (83.1%) Reads that were too short: 2 (0.0%) Reads written (passing filters): 53,333 (83.1%) Total basepairs processed: 17,540,497 bp Total written (filtered): 14,511,150 bp (82.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 53335 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 3 0.0 3 0 0 0 3 18 43 0.0 3 0 0 34 7 2 19 724 0.0 3 0 687 21 9 7 20 52262 0.0 4 50443 1483 224 81 31 21 143 0.0 4 26 110 5 2 22 15 0.0 4 2 1 10 0 2 23 136 0.0 4 75 5 3 44 9 24 6 0.0 4 2 1 0 2 1 28 1 0.0 4 1 77 1 0.0 4 0 0 0 0 1 99 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.17 s (41 us/read; 1.47 M reads/minute). === Summary === Total reads processed: 53,333 Reads with adapters: 3,572 (6.7%) Reads written (passing filters): 53,333 (100.0%) Total basepairs processed: 14,511,150 bp Total written (filtered): 14,453,895 bp (99.6%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 3572 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 8.7% C: 2.2% G: 87.3% T: 1.7% none/other: 0.1% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 833.3 0 1 4 5 208.3 0 5 9 1598 0.2 0 109 1489 10 879 0.1 1 27 852 11 963 0.0 1 1 962 12 10 0.0 1 0 10 13 6 0.0 1 0 6 105 1 0.0 1 1 121 2 0.0 1 1 1 150 3 0.0 1 1 2 151 1 0.0 1 0 1 189 2 0.0 1 0 2 193 2 0.0 1 2 208 69 0.0 1 59 10 209 4 0.0 1 3 1 210 2 0.0 1 1 1 211 2 0.0 1 1 1 223 5 0.0 1 5 224 1 0.0 1 1 231 1 0.0 1 1 257 1 0.0 1 1 262 10 0.0 1 10 267 1 0.0 1 1 268 1 0.0 1 1 272 1 0.0 1 1 273 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.