This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ACTTCA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 99.33 s (31 us/read; 1.93 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 280,952 (8.8%) Reads that were too short: 62,809 (2.0%) Reads written (passing filters): 218,143 (6.8%) Total basepairs processed: 961,105,341 bp Total written (filtered): 60,125,148 bp (6.3%) === Adapter 1 === Sequence: ACTTCA; Type: regular 5'; Length: 6; Trimmed: 280952 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 3927 779.6 0 3927 7 132007 779.6 0 132007 8 1072 779.6 0 1072 9 49076 779.6 0 49076 10 72 779.6 0 72 11 8 779.6 0 8 12 7 779.6 0 7 13 3 779.6 0 3 14 11 779.6 0 11 15 15 779.6 0 15 16 27 779.6 0 27 17 36 779.6 0 36 18 11 779.6 0 11 19 3 779.6 0 3 20 7 779.6 0 7 21 9 779.6 0 9 22 9 779.6 0 9 23 10 779.6 0 10 24 13 779.6 0 13 25 9 779.6 0 9 26 23 779.6 0 23 27 1407 779.6 0 1407 28 110 779.6 0 110 29 11 779.6 0 11 30 6 779.6 0 6 31 12 779.6 0 12 32 7 779.6 0 7 33 12 779.6 0 12 34 7 779.6 0 7 35 4 779.6 0 4 36 4 779.6 0 4 37 8 779.6 0 8 38 12 779.6 0 12 39 7 779.6 0 7 40 21 779.6 0 21 41 15 779.6 0 15 42 11 779.6 0 11 43 9 779.6 0 9 44 4 779.6 0 4 45 14 779.6 0 14 46 9 779.6 0 9 47 7 779.6 0 7 48 11 779.6 0 11 49 5 779.6 0 5 50 7 779.6 0 7 51 8 779.6 0 8 52 6 779.6 0 6 53 16 779.6 0 16 54 20 779.6 0 20 55 40 779.6 0 40 56 34 779.6 0 34 57 601 779.6 0 601 58 559 779.6 0 559 59 924 779.6 0 924 60 18 779.6 0 18 61 5 779.6 0 5 62 9 779.6 0 9 63 20 779.6 0 20 64 9 779.6 0 9 65 10 779.6 0 10 66 4 779.6 0 4 67 9 779.6 0 9 68 15 779.6 0 15 69 6 779.6 0 6 70 10 779.6 0 10 71 2 779.6 0 2 72 8 779.6 0 8 73 12 779.6 0 12 74 4 779.6 0 4 75 6 779.6 0 6 76 17 779.6 0 17 77 13 779.6 0 13 78 15 779.6 0 15 79 37 779.6 0 37 80 82 779.6 0 82 81 61 779.6 0 61 82 119 779.6 0 119 83 74 779.6 0 74 84 206 779.6 0 206 85 124 779.6 0 124 86 90 779.6 0 90 87 23 779.6 0 23 88 313 779.6 0 313 89 452 779.6 0 452 90 1243 779.6 0 1243 91 112 779.6 0 112 92 46 779.6 0 46 93 52 779.6 0 52 94 128 779.6 0 128 95 157 779.6 0 157 96 84 779.6 0 84 97 13 779.6 0 13 98 29 779.6 0 29 99 61 779.6 0 61 100 269 779.6 0 269 101 403 779.6 0 403 102 108 779.6 0 108 103 46 779.6 0 46 104 55 779.6 0 55 105 303 779.6 0 303 106 373 779.6 0 373 107 97 779.6 0 97 108 43 779.6 0 43 109 49 779.6 0 49 110 81 779.6 0 81 111 66 779.6 0 66 112 74 779.6 0 74 113 34 779.6 0 34 114 48 779.6 0 48 115 99 779.6 0 99 116 75 779.6 0 75 117 178 779.6 0 178 118 63 779.6 0 63 119 71 779.6 0 71 120 101 779.6 0 101 121 185 779.6 0 185 122 481 779.6 0 481 123 799 779.6 0 799 124 540 779.6 0 540 125 77 779.6 0 77 126 106 779.6 0 106 127 129 779.6 0 129 128 24 779.6 0 24 129 87 779.6 0 87 130 117 779.6 0 117 131 169 779.6 0 169 132 211 779.6 0 211 133 82 779.6 0 82 134 195 779.6 0 195 135 136 779.6 0 136 136 200 779.6 0 200 137 230 779.6 0 230 138 141 779.6 0 141 139 134 779.6 0 134 140 231 779.6 0 231 141 881 779.6 0 881 142 296 779.6 0 296 143 235 779.6 0 235 144 71 779.6 0 71 145 138 779.6 0 138 146 149 779.6 0 149 147 517 779.6 0 517 148 429 779.6 0 429 149 370 779.6 0 370 150 192 779.6 0 192 151 2007 779.6 0 2007 152 640 779.6 0 640 153 1643 779.6 0 1643 154 278 779.6 0 278 155 304 779.6 0 304 156 226 779.6 0 226 157 858 779.6 0 858 158 709 779.6 0 709 159 632 779.6 0 632 160 183 779.6 0 183 161 189 779.6 0 189 162 215 779.6 0 215 163 224 779.6 0 224 164 133 779.6 0 133 165 137 779.6 0 137 166 160 779.6 0 160 167 116 779.6 0 116 168 164 779.6 0 164 169 303 779.6 0 303 170 133 779.6 0 133 171 129 779.6 0 129 172 55 779.6 0 55 173 66 779.6 0 66 174 182 779.6 0 182 175 123 779.6 0 123 176 101 779.6 0 101 177 74 779.6 0 74 178 46 779.6 0 46 179 85 779.6 0 85 180 153 779.6 0 153 181 168 779.6 0 168 182 352 779.6 0 352 183 506 779.6 0 506 184 439 779.6 0 439 185 262 779.6 0 262 186 230 779.6 0 230 187 233 779.6 0 233 188 71 779.6 0 71 189 65 779.6 0 65 190 71 779.6 0 71 191 131 779.6 0 131 192 176 779.6 0 176 193 121 779.6 0 121 194 75 779.6 0 75 195 80 779.6 0 80 196 143 779.6 0 143 197 109 779.6 0 109 198 228 779.6 0 228 199 92 779.6 0 92 200 60 779.6 0 60 201 94 779.6 0 94 202 85 779.6 0 85 203 165 779.6 0 165 204 267 779.6 0 267 205 268 779.6 0 268 206 148 779.6 0 148 207 166 779.6 0 166 208 302 779.6 0 302 209 552 779.6 0 552 210 597 779.6 0 597 211 829 779.6 0 829 212 882 779.6 0 882 213 7714 779.6 0 7714 214 7186 779.6 0 7186 215 21664 779.6 0 21664 216 627 779.6 0 627 217 243 779.6 0 243 218 211 779.6 0 211 219 202 779.6 0 202 220 113 779.6 0 113 221 494 779.6 0 494 222 1535 779.6 0 1535 223 1358 779.6 0 1358 224 333 779.6 0 333 225 199 779.6 0 199 226 339 779.6 0 339 227 199 779.6 0 199 228 164 779.6 0 164 229 173 779.6 0 173 230 128 779.6 0 128 231 92 779.6 0 92 232 42 779.6 0 42 233 102 779.6 0 102 234 155 779.6 0 155 235 239 779.6 0 239 236 244 779.6 0 244 237 100 779.6 0 100 238 86 779.6 0 86 239 182 779.6 0 182 240 42 779.6 0 42 241 47 779.6 0 47 242 109 779.6 0 109 243 261 779.6 0 261 244 535 779.6 0 535 245 337 779.6 0 337 246 182 779.6 0 182 247 116 779.6 0 116 248 74 779.6 0 74 249 25 779.6 0 25 250 24 779.6 0 24 251 67 779.6 0 67 252 264 779.6 0 264 253 456 779.6 0 456 254 250 779.6 0 250 255 218 779.6 0 218 256 480 779.6 0 480 257 1191 779.6 0 1191 258 718 779.6 0 718 259 248 779.6 0 248 260 53 779.6 0 53 261 94 779.6 0 94 262 163 779.6 0 163 263 252 779.6 0 252 264 376 779.6 0 376 265 395 779.6 0 395 266 244 779.6 0 244 267 248 779.6 0 248 268 132 779.6 0 132 269 69 779.6 0 69 270 55 779.6 0 55 271 166 779.6 0 166 272 410 779.6 0 410 273 428 779.6 0 428 274 294 779.6 0 294 275 247 779.6 0 247 276 123 779.6 0 123 277 97 779.6 0 97 278 155 779.6 0 155 279 502 779.6 0 502 280 266 779.6 0 266 281 201 779.6 0 201 282 124 779.6 0 124 283 204 779.6 0 204 284 489 779.6 0 489 285 329 779.6 0 329 286 333 779.6 0 333 287 184 779.6 0 184 288 110 779.6 0 110 289 38 779.6 0 38 290 52 779.6 0 52 291 275 779.6 0 275 292 315 779.6 0 315 293 350 779.6 0 350 294 245 779.6 0 245 295 91 779.6 0 91 296 78 779.6 0 78 297 56 779.6 0 56 298 53 779.6 0 53 299 117 779.6 0 117 300 179 779.6 0 179 301 188 779.6 0 188 Finished in 10.89 s (50 us/read; 1.20 M reads/minute). === Summary === Total reads processed: 218,143 Reads with adapters: 50,630 (23.2%) Reads that were too short: 77 (0.0%) Reads written (passing filters): 50,553 (23.2%) Total basepairs processed: 60,125,148 bp Total written (filtered): 13,899,175 bp (23.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 50630 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 1 0.0 2 0 0 0 1 15 14 0.0 3 0 0 14 16 388 0.0 3 0 378 9 1 17 49868 0.0 3 48814 1016 35 3 18 120 0.0 3 6 111 2 1 19 26 0.0 3 2 0 21 3 20 103 0.0 3 0 0 0 103 24 1 0.0 3 1 37 1 0.0 3 0 0 0 1 45 2 0.0 3 0 0 0 2 55 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 84 1 0.0 3 0 0 0 1 96 1 0.0 3 0 0 0 1 104 1 0.0 3 0 0 0 1 111 3 0.0 3 0 0 0 3 142 1 0.0 3 0 0 0 1 145 2 0.0 3 0 0 0 2 160 8 0.0 3 0 0 0 8 164 8 0.0 3 0 0 0 8 172 1 0.0 3 0 0 0 1 183 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 195 1 0.0 3 0 0 0 1 197 1 0.0 3 0 0 0 1 217 57 0.0 3 0 0 0 57 281 2 0.0 3 0 0 0 2 282 9 0.0 3 0 0 0 9 283 2 0.0 3 0 0 0 2 289 2 0.0 3 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 2.31 s (46 us/read; 1.31 M reads/minute). === Summary === Total reads processed: 50,553 Reads with adapters: 15,201 (30.1%) Reads written (passing filters): 50,553 (100.0%) Total basepairs processed: 13,899,175 bp Total written (filtered): 13,853,563 bp (99.7%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 15201 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 95.5% C: 2.2% G: 2.0% T: 0.3% none/other: 0.0% WARNING: The adapter is preceded by "A" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "A" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 15192 789.9 0 15192 4 9 197.5 0 9 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.