This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g GAACTA -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 100.29 s (31 us/read; 1.91 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 165,311 (5.2%) Reads that were too short: 42,609 (1.3%) Reads written (passing filters): 122,702 (3.8%) Total basepairs processed: 961,105,341 bp Total written (filtered): 31,152,067 bp (3.2%) === Adapter 1 === Sequence: GAACTA; Type: regular 5'; Length: 6; Trimmed: 165311 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 763 779.6 0 763 7 2757 779.6 0 2757 8 84510 779.6 0 84510 9 107 779.6 0 107 10 36 779.6 0 36 11 148 779.6 0 148 12 42 779.6 0 42 13 174 779.6 0 174 14 115 779.6 0 115 15 122 779.6 0 122 16 35 779.6 0 35 17 10 779.6 0 10 18 6 779.6 0 6 19 5 779.6 0 5 20 4 779.6 0 4 21 4 779.6 0 4 22 2 779.6 0 2 23 7 779.6 0 7 24 9 779.6 0 9 25 27 779.6 0 27 26 43 779.6 0 43 27 34 779.6 0 34 28 5 779.6 0 5 29 3 779.6 0 3 30 4 779.6 0 4 31 3 779.6 0 3 32 4 779.6 0 4 33 5 779.6 0 5 34 4 779.6 0 4 35 6 779.6 0 6 36 4 779.6 0 4 37 2 779.6 0 2 38 18 779.6 0 18 39 3 779.6 0 3 40 2 779.6 0 2 41 8 779.6 0 8 42 4 779.6 0 4 43 6 779.6 0 6 44 5 779.6 0 5 45 4 779.6 0 4 46 4 779.6 0 4 47 8 779.6 0 8 48 11 779.6 0 11 49 8 779.6 0 8 50 12 779.6 0 12 51 5 779.6 0 5 52 3 779.6 0 3 53 4 779.6 0 4 54 5 779.6 0 5 55 7 779.6 0 7 56 10 779.6 0 10 57 11 779.6 0 11 58 11 779.6 0 11 59 24 779.6 0 24 60 19 779.6 0 19 61 10 779.6 0 10 62 2 779.6 0 2 63 2 779.6 0 2 64 6 779.6 0 6 65 7 779.6 0 7 66 7 779.6 0 7 67 5 779.6 0 5 68 1 779.6 0 1 69 2 779.6 0 2 70 9 779.6 0 9 71 15 779.6 0 15 72 2 779.6 0 2 73 7 779.6 0 7 74 15 779.6 0 15 75 34 779.6 0 34 76 113 779.6 0 113 77 14 779.6 0 14 78 104 779.6 0 104 79 134 779.6 0 134 80 140 779.6 0 140 81 118 779.6 0 118 82 575 779.6 0 575 83 216 779.6 0 216 84 573 779.6 0 573 85 242 779.6 0 242 86 73 779.6 0 73 87 80 779.6 0 80 88 445 779.6 0 445 89 816 779.6 0 816 90 80 779.6 0 80 91 91 779.6 0 91 92 165 779.6 0 165 93 92 779.6 0 92 94 164 779.6 0 164 95 115 779.6 0 115 96 81 779.6 0 81 97 100 779.6 0 100 98 234 779.6 0 234 99 236 779.6 0 236 100 87 779.6 0 87 101 50 779.6 0 50 102 210 779.6 0 210 103 70 779.6 0 70 104 128 779.6 0 128 105 299 779.6 0 299 106 104 779.6 0 104 107 43 779.6 0 43 108 75 779.6 0 75 109 160 779.6 0 160 110 308 779.6 0 308 111 275 779.6 0 275 112 452 779.6 0 452 113 129 779.6 0 129 114 121 779.6 0 121 115 110 779.6 0 110 116 74 779.6 0 74 117 88 779.6 0 88 118 256 779.6 0 256 119 151 779.6 0 151 120 197 779.6 0 197 121 238 779.6 0 238 122 173 779.6 0 173 123 200 779.6 0 200 124 182 779.6 0 182 125 164 779.6 0 164 126 149 779.6 0 149 127 139 779.6 0 139 128 122 779.6 0 122 129 190 779.6 0 190 130 178 779.6 0 178 131 121 779.6 0 121 132 179 779.6 0 179 133 221 779.6 0 221 134 174 779.6 0 174 135 197 779.6 0 197 136 180 779.6 0 180 137 201 779.6 0 201 138 346 779.6 0 346 139 367 779.6 0 367 140 508 779.6 0 508 141 203 779.6 0 203 142 174 779.6 0 174 143 137 779.6 0 137 144 269 779.6 0 269 145 195 779.6 0 195 146 194 779.6 0 194 147 241 779.6 0 241 148 183 779.6 0 183 149 146 779.6 0 146 150 113 779.6 0 113 151 136 779.6 0 136 152 195 779.6 0 195 153 203 779.6 0 203 154 160 779.6 0 160 155 176 779.6 0 176 156 213 779.6 0 213 157 303 779.6 0 303 158 211 779.6 0 211 159 271 779.6 0 271 160 719 779.6 0 719 161 416 779.6 0 416 162 448 779.6 0 448 163 217 779.6 0 217 164 185 779.6 0 185 165 174 779.6 0 174 166 145 779.6 0 145 167 140 779.6 0 140 168 193 779.6 0 193 169 329 779.6 0 329 170 126 779.6 0 126 171 137 779.6 0 137 172 236 779.6 0 236 173 328 779.6 0 328 174 116 779.6 0 116 175 142 779.6 0 142 176 131 779.6 0 131 177 214 779.6 0 214 178 259 779.6 0 259 179 316 779.6 0 316 180 285 779.6 0 285 181 349 779.6 0 349 182 407 779.6 0 407 183 329 779.6 0 329 184 293 779.6 0 293 185 350 779.6 0 350 186 301 779.6 0 301 187 2131 779.6 0 2131 188 1083 779.6 0 1083 189 3644 779.6 0 3644 190 359 779.6 0 359 191 285 779.6 0 285 192 101 779.6 0 101 193 152 779.6 0 152 194 132 779.6 0 132 195 76 779.6 0 76 196 261 779.6 0 261 197 538 779.6 0 538 198 228 779.6 0 228 199 133 779.6 0 133 200 255 779.6 0 255 201 383 779.6 0 383 202 544 779.6 0 544 203 758 779.6 0 758 204 266 779.6 0 266 205 265 779.6 0 265 206 280 779.6 0 280 207 228 779.6 0 228 208 228 779.6 0 228 209 619 779.6 0 619 210 440 779.6 0 440 211 274 779.6 0 274 212 303 779.6 0 303 213 274 779.6 0 274 214 216 779.6 0 216 215 185 779.6 0 185 216 258 779.6 0 258 217 279 779.6 0 279 218 243 779.6 0 243 219 251 779.6 0 251 220 230 779.6 0 230 221 325 779.6 0 325 222 481 779.6 0 481 223 365 779.6 0 365 224 216 779.6 0 216 225 186 779.6 0 186 226 223 779.6 0 223 227 317 779.6 0 317 228 224 779.6 0 224 229 378 779.6 0 378 230 624 779.6 0 624 231 879 779.6 0 879 232 602 779.6 0 602 233 431 779.6 0 431 234 304 779.6 0 304 235 170 779.6 0 170 236 146 779.6 0 146 237 294 779.6 0 294 238 225 779.6 0 225 239 953 779.6 0 953 240 558 779.6 0 558 241 425 779.6 0 425 242 317 779.6 0 317 243 285 779.6 0 285 244 247 779.6 0 247 245 290 779.6 0 290 246 285 779.6 0 285 247 155 779.6 0 155 248 140 779.6 0 140 249 142 779.6 0 142 250 235 779.6 0 235 251 369 779.6 0 369 252 289 779.6 0 289 253 332 779.6 0 332 254 440 779.6 0 440 255 474 779.6 0 474 256 178 779.6 0 178 257 148 779.6 0 148 258 177 779.6 0 177 259 197 779.6 0 197 260 224 779.6 0 224 261 220 779.6 0 220 262 196 779.6 0 196 263 245 779.6 0 245 264 332 779.6 0 332 265 424 779.6 0 424 266 271 779.6 0 271 267 187 779.6 0 187 268 171 779.6 0 171 269 207 779.6 0 207 270 372 779.6 0 372 271 302 779.6 0 302 272 356 779.6 0 356 273 376 779.6 0 376 274 334 779.6 0 334 275 732 779.6 0 732 276 766 779.6 0 766 277 2381 779.6 0 2381 278 543 779.6 0 543 279 695 779.6 0 695 280 849 779.6 0 849 281 523 779.6 0 523 282 573 779.6 0 573 283 553 779.6 0 553 284 866 779.6 0 866 285 920 779.6 0 920 286 350 779.6 0 350 287 305 779.6 0 305 288 857 779.6 0 857 289 522 779.6 0 522 290 332 779.6 0 332 291 371 779.6 0 371 292 508 779.6 0 508 293 345 779.6 0 345 294 300 779.6 0 300 295 453 779.6 0 453 296 483 779.6 0 483 297 373 779.6 0 373 298 704 779.6 0 704 299 658 779.6 0 658 300 1867 779.6 0 1867 301 1396 779.6 0 1396 Finished in 5.69 s (46 us/read; 1.29 M reads/minute). === Summary === Total reads processed: 122,702 Reads with adapters: 87,725 (71.5%) Reads that were too short: 34 (0.0%) Reads written (passing filters): 87,691 (71.5%) Total basepairs processed: 31,152,067 bp Total written (filtered): 23,942,812 bp (76.9%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 87725 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 5 0.0 3 0 0 0 3 2 18 28 0.0 3 0 0 22 4 2 19 778 0.0 3 0 688 37 30 23 20 86460 0.0 4 83700 2412 262 61 25 21 302 0.0 4 79 216 4 2 1 22 24 0.0 4 0 1 13 0 10 23 86 0.0 4 1 0 1 69 15 24 2 0.0 4 1 0 0 0 1 25 1 0.0 4 0 0 1 26 1 0.0 4 1 30 1 0.0 4 1 38 1 0.0 4 0 0 0 0 1 41 1 0.0 4 0 0 0 0 1 49 1 0.0 4 0 0 0 0 1 50 1 0.0 4 0 0 0 0 1 66 1 0.0 4 0 0 0 0 1 70 1 0.0 4 0 0 0 0 1 82 1 0.0 4 0 0 0 0 1 85 1 0.0 4 0 0 0 0 1 93 1 0.0 4 0 0 0 0 1 107 1 0.0 4 0 0 0 0 1 122 1 0.0 4 0 0 0 0 1 128 1 0.0 4 0 0 0 1 133 1 0.0 4 0 0 0 0 1 135 1 0.0 4 0 0 0 0 1 149 1 0.0 4 0 0 0 0 1 150 1 0.0 4 0 0 0 0 1 152 1 0.0 4 0 0 0 0 1 154 1 0.0 4 0 0 0 0 1 160 1 0.0 4 0 0 0 0 1 174 2 0.0 4 0 0 0 0 2 183 1 0.0 4 0 0 0 0 1 184 13 0.0 4 0 0 0 1 12 186 2 0.0 4 0 0 0 0 2 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.68 s (42 us/read; 1.43 M reads/minute). === Summary === Total reads processed: 87,691 Reads with adapters: 19,163 (21.9%) Reads written (passing filters): 87,691 (100.0%) Total basepairs processed: 23,942,812 bp Total written (filtered): 23,721,459 bp (99.1%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 19163 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.1% C: 1.5% G: 94.8% T: 1.6% none/other: 0.0% WARNING: The adapter is preceded by "G" extremely often. The provided adapter sequence may be incomplete. To fix the problem, add "G" to the beginning of the adapter sequence. Overview of removed sequences length count expect max.err error counts 3 1 1370.2 0 1 4 3 342.5 0 3 9 7 0.3 0 0 7 10 349 0.1 1 16 333 11 18732 0.0 1 46 18686 12 7 0.0 1 0 7 13 5 0.0 1 0 5 14 1 0.0 1 0 1 15 1 0.0 1 0 1 73 2 0.0 1 2 151 2 0.0 1 0 2 152 1 0.0 1 0 1 190 1 0.0 1 0 1 209 45 0.0 1 25 20 210 2 0.0 1 1 1 211 2 0.0 1 1 1 258 1 0.0 1 1 260 1 0.0 1 1 WARNING: One or more of your adapter sequences may be incomplete. Please see the detailed output above.