This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCCAGC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 101.63 s (32 us/read; 1.89 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 407,327 (12.8%) Reads that were too short: 4,870 (0.2%) Reads written (passing filters): 402,457 (12.6%) Total basepairs processed: 961,105,341 bp Total written (filtered): 96,553,445 bp (10.0%) === Adapter 1 === Sequence: TCCAGC; Type: regular 5'; Length: 6; Trimmed: 407327 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1129 779.6 0 1129 7 2413 779.6 0 2413 8 83489 779.6 0 83489 9 10027 779.6 0 10027 10 29 779.6 0 29 11 35 779.6 0 35 12 27 779.6 0 27 13 14 779.6 0 14 14 14 779.6 0 14 15 17 779.6 0 17 16 9 779.6 0 9 17 17 779.6 0 17 18 34 779.6 0 34 19 32 779.6 0 32 20 42 779.6 0 42 21 26 779.6 0 26 22 38 779.6 0 38 23 54 779.6 0 54 24 53 779.6 0 53 25 20 779.6 0 20 26 18 779.6 0 18 27 19 779.6 0 19 28 18 779.6 0 18 29 516 779.6 0 516 30 195 779.6 0 195 31 26 779.6 0 26 32 26 779.6 0 26 33 37 779.6 0 37 34 23 779.6 0 23 35 44 779.6 0 44 36 101 779.6 0 101 37 71 779.6 0 71 38 245 779.6 0 245 39 101 779.6 0 101 40 69 779.6 0 69 41 25 779.6 0 25 42 88 779.6 0 88 43 78 779.6 0 78 44 157 779.6 0 157 45 235 779.6 0 235 46 328 779.6 0 328 47 437 779.6 0 437 48 1012 779.6 0 1012 49 2209 779.6 0 2209 50 494 779.6 0 494 51 657 779.6 0 657 52 1487 779.6 0 1487 53 680 779.6 0 680 54 605 779.6 0 605 55 946 779.6 0 946 56 892 779.6 0 892 57 1077 779.6 0 1077 58 1076 779.6 0 1076 59 1450 779.6 0 1450 60 1719 779.6 0 1719 61 3737 779.6 0 3737 62 63137 779.6 0 63137 63 60885 779.6 0 60885 64 100966 779.6 0 100966 65 1639 779.6 0 1639 66 31 779.6 0 31 67 22 779.6 0 22 68 20 779.6 0 20 69 29 779.6 0 29 70 60 779.6 0 60 71 137 779.6 0 137 72 111 779.6 0 111 73 86 779.6 0 86 74 147 779.6 0 147 75 143 779.6 0 143 76 2425 779.6 0 2425 77 1996 779.6 0 1996 78 5222 779.6 0 5222 79 32 779.6 0 32 80 13 779.6 0 13 81 14 779.6 0 14 82 12 779.6 0 12 83 32 779.6 0 32 84 18 779.6 0 18 85 23 779.6 0 23 86 10 779.6 0 10 87 22 779.6 0 22 88 15 779.6 0 15 89 11 779.6 0 11 90 6 779.6 0 6 91 6 779.6 0 6 92 18 779.6 0 18 93 11 779.6 0 11 94 12 779.6 0 12 95 16 779.6 0 16 96 5 779.6 0 5 97 10 779.6 0 10 98 16 779.6 0 16 99 5 779.6 0 5 100 10 779.6 0 10 101 24 779.6 0 24 102 17 779.6 0 17 103 33 779.6 0 33 104 57 779.6 0 57 105 7 779.6 0 7 106 42 779.6 0 42 107 66 779.6 0 66 108 43 779.6 0 43 109 44 779.6 0 44 110 19 779.6 0 19 111 14 779.6 0 14 112 20 779.6 0 20 113 15 779.6 0 15 114 21 779.6 0 21 115 10 779.6 0 10 116 18 779.6 0 18 117 30 779.6 0 30 118 13 779.6 0 13 119 18 779.6 0 18 120 22 779.6 0 22 121 9 779.6 0 9 122 14 779.6 0 14 123 18 779.6 0 18 124 21 779.6 0 21 125 19 779.6 0 19 126 35 779.6 0 35 127 57 779.6 0 57 128 45 779.6 0 45 129 24 779.6 0 24 130 14 779.6 0 14 131 15 779.6 0 15 132 21 779.6 0 21 133 22 779.6 0 22 134 43 779.6 0 43 135 46 779.6 0 46 136 34 779.6 0 34 137 67 779.6 0 67 138 27 779.6 0 27 139 43 779.6 0 43 140 69 779.6 0 69 141 78 779.6 0 78 142 212 779.6 0 212 143 239 779.6 0 239 144 3774 779.6 0 3774 145 1168 779.6 0 1168 146 3530 779.6 0 3530 147 142 779.6 0 142 148 48 779.6 0 48 149 141 779.6 0 141 150 264 779.6 0 264 151 301 779.6 0 301 152 73 779.6 0 73 153 45 779.6 0 45 154 57 779.6 0 57 155 49 779.6 0 49 156 176 779.6 0 176 157 184 779.6 0 184 158 343 779.6 0 343 159 146 779.6 0 146 160 152 779.6 0 152 161 188 779.6 0 188 162 374 779.6 0 374 163 559 779.6 0 559 164 6233 779.6 0 6233 165 4693 779.6 0 4693 166 16774 779.6 0 16774 167 125 779.6 0 125 168 82 779.6 0 82 169 362 779.6 0 362 170 361 779.6 0 361 171 462 779.6 0 462 172 564 779.6 0 564 173 507 779.6 0 507 174 267 779.6 0 267 175 27 779.6 0 27 176 29 779.6 0 29 177 31 779.6 0 31 178 25 779.6 0 25 179 115 779.6 0 115 180 106 779.6 0 106 181 74 779.6 0 74 182 124 779.6 0 124 183 80 779.6 0 80 184 84 779.6 0 84 185 84 779.6 0 84 186 112 779.6 0 112 187 60 779.6 0 60 188 105 779.6 0 105 189 326 779.6 0 326 190 698 779.6 0 698 191 395 779.6 0 395 192 171 779.6 0 171 193 16 779.6 0 16 194 16 779.6 0 16 195 11 779.6 0 11 196 24 779.6 0 24 197 24 779.6 0 24 198 17 779.6 0 17 199 13 779.6 0 13 200 18 779.6 0 18 201 33 779.6 0 33 202 25 779.6 0 25 203 14 779.6 0 14 204 13 779.6 0 13 205 34 779.6 0 34 206 13 779.6 0 13 207 37 779.6 0 37 208 44 779.6 0 44 209 24 779.6 0 24 210 22 779.6 0 22 211 15 779.6 0 15 212 41 779.6 0 41 213 30 779.6 0 30 214 36 779.6 0 36 215 25 779.6 0 25 216 75 779.6 0 75 217 107 779.6 0 107 218 104 779.6 0 104 219 72 779.6 0 72 220 23 779.6 0 23 221 37 779.6 0 37 222 19 779.6 0 19 223 18 779.6 0 18 224 17 779.6 0 17 225 61 779.6 0 61 226 74 779.6 0 74 227 113 779.6 0 113 228 93 779.6 0 93 229 97 779.6 0 97 230 170 779.6 0 170 231 183 779.6 0 183 232 64 779.6 0 64 233 24 779.6 0 24 234 9 779.6 0 9 235 19 779.6 0 19 236 14 779.6 0 14 237 14 779.6 0 14 238 20 779.6 0 20 239 28 779.6 0 28 240 17 779.6 0 17 241 25 779.6 0 25 242 5 779.6 0 5 243 28 779.6 0 28 244 20 779.6 0 20 245 32 779.6 0 32 246 39 779.6 0 39 247 19 779.6 0 19 248 19 779.6 0 19 249 20 779.6 0 20 250 15 779.6 0 15 251 10 779.6 0 10 252 10 779.6 0 10 253 17 779.6 0 17 254 35 779.6 0 35 255 51 779.6 0 51 256 64 779.6 0 64 257 21 779.6 0 21 258 24 779.6 0 24 259 39 779.6 0 39 260 185 779.6 0 185 261 142 779.6 0 142 262 52 779.6 0 52 263 30 779.6 0 30 264 21 779.6 0 21 265 37 779.6 0 37 266 64 779.6 0 64 267 58 779.6 0 58 268 65 779.6 0 65 269 26 779.6 0 26 270 20 779.6 0 20 271 19 779.6 0 19 272 23 779.6 0 23 273 18 779.6 0 18 274 16 779.6 0 16 275 29 779.6 0 29 276 35 779.6 0 35 277 54 779.6 0 54 278 51 779.6 0 51 279 113 779.6 0 113 280 199 779.6 0 199 281 41 779.6 0 41 282 125 779.6 0 125 283 17 779.6 0 17 284 19 779.6 0 19 285 32 779.6 0 32 286 63 779.6 0 63 287 159 779.6 0 159 288 308 779.6 0 308 289 187 779.6 0 187 290 20 779.6 0 20 291 12 779.6 0 12 292 18 779.6 0 18 293 20 779.6 0 20 294 55 779.6 0 55 295 32 779.6 0 32 296 28 779.6 0 28 297 68 779.6 0 68 298 32 779.6 0 32 299 18 779.6 0 18 300 5 779.6 0 5 301 16 779.6 0 16 Finished in 17.82 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 402,457 Reads with adapters: 85,988 (21.4%) Reads that were too short: 73 (0.0%) Reads written (passing filters): 85,915 (21.3%) Total basepairs processed: 96,553,445 bp Total written (filtered): 23,685,531 bp (24.5%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 85988 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 14 4 0.0 2 0 0 0 4 15 23 0.0 3 0 0 20 3 16 591 0.0 3 0 573 17 1 17 84458 0.0 3 83099 1334 21 4 18 196 0.0 3 9 180 5 2 19 101 0.0 3 0 0 90 11 20 335 0.0 3 0 0 0 335 22 2 0.0 3 2 23 1 0.0 3 0 1 27 2 0.0 3 0 0 0 2 28 3 0.0 3 0 0 1 2 29 1 0.0 3 1 31 1 0.0 3 1 32 2 0.0 3 2 35 2 0.0 3 1 0 0 1 38 36 0.0 3 0 0 1 35 43 1 0.0 3 0 0 0 1 65 1 0.0 3 0 0 0 1 75 1 0.0 3 0 0 0 1 77 1 0.0 3 0 0 0 1 78 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 81 1 0.0 3 0 0 0 1 94 1 0.0 3 0 0 0 1 103 1 0.0 3 0 0 0 1 104 3 0.0 3 0 0 0 3 105 1 0.0 3 0 0 0 1 107 1 0.0 3 0 0 0 1 111 1 0.0 3 0 0 0 1 112 1 0.0 3 0 0 0 1 113 1 0.0 3 0 0 0 1 122 1 0.0 3 0 0 0 1 123 1 0.0 3 0 0 0 1 124 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 129 66 0.0 3 0 0 0 66 131 2 0.0 3 0 0 0 2 132 72 0.0 3 0 0 1 71 133 2 0.0 3 0 0 0 2 139 1 0.0 3 0 0 0 1 144 2 0.0 3 0 0 0 2 145 1 0.0 3 0 0 0 1 147 1 0.0 3 0 0 0 1 155 1 0.0 3 0 0 0 1 171 1 0.0 3 0 0 0 1 172 1 0.0 3 0 0 0 1 177 2 0.0 3 0 0 0 2 183 2 0.0 3 0 0 0 2 189 1 0.0 3 0 0 0 1 206 2 0.0 3 0 0 0 2 212 1 0.0 3 0 0 0 1 230 1 0.0 3 0 0 0 1 231 48 0.0 3 0 0 2 46 265 1 0.0 3 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 3.67 s (43 us/read; 1.40 M reads/minute). === Summary === Total reads processed: 85,915 Reads with adapters: 646 (0.8%) Reads written (passing filters): 85,915 (100.0%) Total basepairs processed: 23,685,531 bp Total written (filtered): 23,683,551 bp (100.0%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 646 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 49.2% C: 18.1% G: 26.8% T: 5.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 604 1342.4 0 604 4 42 335.6 0 42