This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TCACTC -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 97.42 s (31 us/read; 1.97 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 84,330 (2.6%) Reads that were too short: 27,184 (0.9%) Reads written (passing filters): 57,146 (1.8%) Total basepairs processed: 961,105,341 bp Total written (filtered): 10,857,957 bp (1.1%) === Adapter 1 === Sequence: TCACTC; Type: regular 5'; Length: 6; Trimmed: 84330 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 1126 779.6 0 1126 7 9524 779.6 0 9524 8 3832 779.6 0 3832 9 21 779.6 0 21 10 11 779.6 0 11 11 25 779.6 0 25 12 23 779.6 0 23 13 28 779.6 0 28 14 23 779.6 0 23 15 26 779.6 0 26 16 20 779.6 0 20 17 7 779.6 0 7 18 15 779.6 0 15 19 15 779.6 0 15 20 26 779.6 0 26 21 18 779.6 0 18 22 17 779.6 0 17 23 22 779.6 0 22 24 16 779.6 0 16 25 33 779.6 0 33 26 11 779.6 0 11 27 31 779.6 0 31 28 26 779.6 0 26 29 23 779.6 0 23 30 23 779.6 0 23 31 37 779.6 0 37 32 438 779.6 0 438 33 45 779.6 0 45 34 22 779.6 0 22 35 26 779.6 0 26 36 27 779.6 0 27 37 16 779.6 0 16 38 91 779.6 0 91 39 29 779.6 0 29 40 51 779.6 0 51 41 27 779.6 0 27 42 93 779.6 0 93 43 15 779.6 0 15 44 12 779.6 0 12 45 30 779.6 0 30 46 21 779.6 0 21 47 13 779.6 0 13 48 17 779.6 0 17 49 22 779.6 0 22 50 16 779.6 0 16 51 15 779.6 0 15 52 22 779.6 0 22 53 105 779.6 0 105 54 128 779.6 0 128 55 83 779.6 0 83 56 18 779.6 0 18 57 48 779.6 0 48 58 19 779.6 0 19 59 16 779.6 0 16 60 49 779.6 0 49 61 14 779.6 0 14 62 23 779.6 0 23 63 17 779.6 0 17 64 15 779.6 0 15 65 16 779.6 0 16 66 114 779.6 0 114 67 32 779.6 0 32 68 35 779.6 0 35 69 35 779.6 0 35 70 32 779.6 0 32 71 21 779.6 0 21 72 32 779.6 0 32 73 22 779.6 0 22 74 21 779.6 0 21 75 20 779.6 0 20 76 34 779.6 0 34 77 20 779.6 0 20 78 44 779.6 0 44 79 48 779.6 0 48 80 36 779.6 0 36 81 42 779.6 0 42 82 74 779.6 0 74 83 41 779.6 0 41 84 24 779.6 0 24 85 35 779.6 0 35 86 50 779.6 0 50 87 60 779.6 0 60 88 59 779.6 0 59 89 89 779.6 0 89 90 64 779.6 0 64 91 212 779.6 0 212 92 135 779.6 0 135 93 142 779.6 0 142 94 90 779.6 0 90 95 82 779.6 0 82 96 38 779.6 0 38 97 38 779.6 0 38 98 58 779.6 0 58 99 52 779.6 0 52 100 26 779.6 0 26 101 86 779.6 0 86 102 111 779.6 0 111 103 230 779.6 0 230 104 269 779.6 0 269 105 353 779.6 0 353 106 29 779.6 0 29 107 51 779.6 0 51 108 165 779.6 0 165 109 150 779.6 0 150 110 34 779.6 0 34 111 154 779.6 0 154 112 147 779.6 0 147 113 320 779.6 0 320 114 137 779.6 0 137 115 108 779.6 0 108 116 68 779.6 0 68 117 101 779.6 0 101 118 53 779.6 0 53 119 81 779.6 0 81 120 132 779.6 0 132 121 154 779.6 0 154 122 110 779.6 0 110 123 209 779.6 0 209 124 211 779.6 0 211 125 1439 779.6 0 1439 126 671 779.6 0 671 127 1123 779.6 0 1123 128 94 779.6 0 94 129 98 779.6 0 98 130 251 779.6 0 251 131 98 779.6 0 98 132 110 779.6 0 110 133 146 779.6 0 146 134 217 779.6 0 217 135 205 779.6 0 205 136 118 779.6 0 118 137 128 779.6 0 128 138 231 779.6 0 231 139 355 779.6 0 355 140 168 779.6 0 168 141 105 779.6 0 105 142 85 779.6 0 85 143 180 779.6 0 180 144 236 779.6 0 236 145 235 779.6 0 235 146 252 779.6 0 252 147 160 779.6 0 160 148 100 779.6 0 100 149 109 779.6 0 109 150 443 779.6 0 443 151 384 779.6 0 384 152 936 779.6 0 936 153 169 779.6 0 169 154 208 779.6 0 208 155 223 779.6 0 223 156 535 779.6 0 535 157 415 779.6 0 415 158 467 779.6 0 467 159 664 779.6 0 664 160 4983 779.6 0 4983 161 3588 779.6 0 3588 162 6456 779.6 0 6456 163 280 779.6 0 280 164 446 779.6 0 446 165 247 779.6 0 247 166 327 779.6 0 327 167 135 779.6 0 135 168 167 779.6 0 167 169 169 779.6 0 169 170 169 779.6 0 169 171 232 779.6 0 232 172 238 779.6 0 238 173 114 779.6 0 114 174 86 779.6 0 86 175 145 779.6 0 145 176 151 779.6 0 151 177 145 779.6 0 145 178 196 779.6 0 196 179 167 779.6 0 167 180 387 779.6 0 387 181 828 779.6 0 828 182 1121 779.6 0 1121 183 331 779.6 0 331 184 188 779.6 0 188 185 188 779.6 0 188 186 201 779.6 0 201 187 155 779.6 0 155 188 318 779.6 0 318 189 270 779.6 0 270 190 183 779.6 0 183 191 162 779.6 0 162 192 97 779.6 0 97 193 67 779.6 0 67 194 48 779.6 0 48 195 70 779.6 0 70 196 133 779.6 0 133 197 150 779.6 0 150 198 68 779.6 0 68 199 54 779.6 0 54 200 77 779.6 0 77 201 253 779.6 0 253 202 187 779.6 0 187 203 97 779.6 0 97 204 84 779.6 0 84 205 137 779.6 0 137 206 102 779.6 0 102 207 193 779.6 0 193 208 243 779.6 0 243 209 230 779.6 0 230 210 132 779.6 0 132 211 227 779.6 0 227 212 830 779.6 0 830 213 977 779.6 0 977 214 874 779.6 0 874 215 192 779.6 0 192 216 282 779.6 0 282 217 318 779.6 0 318 218 288 779.6 0 288 219 121 779.6 0 121 220 117 779.6 0 117 221 292 779.6 0 292 222 267 779.6 0 267 223 146 779.6 0 146 224 192 779.6 0 192 225 531 779.6 0 531 226 270 779.6 0 270 227 212 779.6 0 212 228 200 779.6 0 200 229 190 779.6 0 190 230 104 779.6 0 104 231 66 779.6 0 66 232 126 779.6 0 126 233 167 779.6 0 167 234 135 779.6 0 135 235 70 779.6 0 70 236 120 779.6 0 120 237 200 779.6 0 200 238 114 779.6 0 114 239 203 779.6 0 203 240 233 779.6 0 233 241 264 779.6 0 264 242 248 779.6 0 248 243 250 779.6 0 250 244 217 779.6 0 217 245 282 779.6 0 282 246 399 779.6 0 399 247 284 779.6 0 284 248 167 779.6 0 167 249 157 779.6 0 157 250 180 779.6 0 180 251 268 779.6 0 268 252 228 779.6 0 228 253 257 779.6 0 257 254 510 779.6 0 510 255 1158 779.6 0 1158 256 1111 779.6 0 1111 257 513 779.6 0 513 258 246 779.6 0 246 259 119 779.6 0 119 260 141 779.6 0 141 261 64 779.6 0 64 262 113 779.6 0 113 263 194 779.6 0 194 264 201 779.6 0 201 265 224 779.6 0 224 266 468 779.6 0 468 267 523 779.6 0 523 268 421 779.6 0 421 269 280 779.6 0 280 270 323 779.6 0 323 271 669 779.6 0 669 272 404 779.6 0 404 273 485 779.6 0 485 274 410 779.6 0 410 275 577 779.6 0 577 276 236 779.6 0 236 277 281 779.6 0 281 278 290 779.6 0 290 279 153 779.6 0 153 280 237 779.6 0 237 281 197 779.6 0 197 282 508 779.6 0 508 283 298 779.6 0 298 284 261 779.6 0 261 285 108 779.6 0 108 286 181 779.6 0 181 287 395 779.6 0 395 288 382 779.6 0 382 289 193 779.6 0 193 290 171 779.6 0 171 291 182 779.6 0 182 292 262 779.6 0 262 293 138 779.6 0 138 294 84 779.6 0 84 295 113 779.6 0 113 296 117 779.6 0 117 297 93 779.6 0 93 298 202 779.6 0 202 299 128 779.6 0 128 300 114 779.6 0 114 301 36 779.6 0 36 Finished in 2.52 s (44 us/read; 1.36 M reads/minute). === Summary === Total reads processed: 57,146 Reads with adapters: 10,599 (18.5%) Reads that were too short: 12 (0.0%) Reads written (passing filters): 10,587 (18.5%) Total basepairs processed: 10,857,957 bp Total written (filtered): 2,893,922 bp (26.7%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 10599 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 18 20 0.0 3 0 0 7 2 11 19 155 0.0 3 0 148 2 3 2 20 9735 0.0 4 9535 177 16 6 1 21 617 0.0 4 586 31 22 3 0.0 4 0 1 1 0 1 23 5 0.0 4 0 0 0 3 2 28 1 0.0 4 0 0 0 0 1 35 2 0.0 4 0 0 0 0 2 36 1 0.0 4 0 0 0 0 1 44 1 0.0 4 0 0 0 0 1 53 1 0.0 4 1 62 1 0.0 4 0 0 0 0 1 72 1 0.0 4 0 0 0 0 1 75 1 0.0 4 0 0 0 0 1 78 1 0.0 4 0 0 0 0 1 80 1 0.0 4 0 0 0 0 1 115 1 0.0 4 0 0 0 0 1 121 1 0.0 4 0 0 0 0 1 127 1 0.0 4 0 0 0 0 1 128 45 0.0 4 0 0 0 0 45 161 1 0.0 4 0 0 0 1 170 1 0.0 4 0 0 0 1 187 1 0.0 4 0 0 0 0 1 204 1 0.0 4 0 0 0 0 1 252 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.42 s (40 us/read; 1.51 M reads/minute). === Summary === Total reads processed: 10,587 Reads with adapters: 475 (4.5%) Reads written (passing filters): 10,587 (100.0%) Total basepairs processed: 2,893,922 bp Total written (filtered): 2,811,749 bp (97.2%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 475 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 2.7% C: 1.1% G: 34.1% T: 62.1% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 2 165.4 0 2 4 1 41.4 0 1 9 1 0.0 0 0 1 11 157 0.0 1 1 156 12 13 0.0 1 0 13 152 3 0.0 1 3 210 7 0.0 1 3 4 264 1 0.0 1 1 269 221 0.0 1 215 6 270 67 0.0 1 64 3 271 2 0.0 1 2