This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g CTTGGT -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... Finished in 102.19 s (32 us/read; 1.87 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 60,537 (1.9%) Reads that were too short: 5,249 (0.2%) Reads written (passing filters): 55,288 (1.7%) Total basepairs processed: 961,105,341 bp Total written (filtered): 12,269,414 bp (1.3%) === Adapter 1 === Sequence: CTTGGT; Type: regular 5'; Length: 6; Trimmed: 60537 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 6047 779.6 0 6047 7 11571 779.6 0 11571 8 10777 779.6 0 10777 9 29 779.6 0 29 10 15 779.6 0 15 11 13 779.6 0 13 12 17 779.6 0 17 13 8 779.6 0 8 14 10 779.6 0 10 15 13 779.6 0 13 16 12 779.6 0 12 17 15 779.6 0 15 18 17 779.6 0 17 19 79 779.6 0 79 20 77 779.6 0 77 21 151 779.6 0 151 22 24 779.6 0 24 23 13 779.6 0 13 24 14 779.6 0 14 25 12 779.6 0 12 26 11 779.6 0 11 27 16 779.6 0 16 28 13 779.6 0 13 29 14 779.6 0 14 30 17 779.6 0 17 31 16 779.6 0 16 32 13 779.6 0 13 33 11 779.6 0 11 34 25 779.6 0 25 35 23 779.6 0 23 36 11 779.6 0 11 37 125 779.6 0 125 38 123 779.6 0 123 39 54 779.6 0 54 40 24 779.6 0 24 41 10 779.6 0 10 42 8 779.6 0 8 43 9 779.6 0 9 44 12 779.6 0 12 45 20 779.6 0 20 46 21 779.6 0 21 47 12 779.6 0 12 48 13 779.6 0 13 49 19 779.6 0 19 50 10 779.6 0 10 51 14 779.6 0 14 52 13 779.6 0 13 53 6 779.6 0 6 54 10 779.6 0 10 55 12 779.6 0 12 56 21 779.6 0 21 57 17 779.6 0 17 58 14 779.6 0 14 59 11 779.6 0 11 60 15 779.6 0 15 61 18 779.6 0 18 62 10 779.6 0 10 63 17 779.6 0 17 64 19 779.6 0 19 65 14 779.6 0 14 66 11 779.6 0 11 67 8 779.6 0 8 68 7 779.6 0 7 69 14 779.6 0 14 70 8 779.6 0 8 71 15 779.6 0 15 72 31 779.6 0 31 73 26 779.6 0 26 74 11 779.6 0 11 75 13 779.6 0 13 76 71 779.6 0 71 77 107 779.6 0 107 78 139 779.6 0 139 79 16 779.6 0 16 80 31 779.6 0 31 81 19 779.6 0 19 82 8 779.6 0 8 83 10 779.6 0 10 84 16 779.6 0 16 85 7 779.6 0 7 86 13 779.6 0 13 87 12 779.6 0 12 88 12 779.6 0 12 89 10 779.6 0 10 90 15 779.6 0 15 91 21 779.6 0 21 92 96 779.6 0 96 93 88 779.6 0 88 94 26 779.6 0 26 95 31 779.6 0 31 96 45 779.6 0 45 97 144 779.6 0 144 98 196 779.6 0 196 99 12 779.6 0 12 100 11 779.6 0 11 101 17 779.6 0 17 102 7 779.6 0 7 103 11 779.6 0 11 104 14 779.6 0 14 105 25 779.6 0 25 106 16 779.6 0 16 107 19 779.6 0 19 108 19 779.6 0 19 109 15 779.6 0 15 110 11 779.6 0 11 111 8 779.6 0 8 112 10 779.6 0 10 113 6 779.6 0 6 114 13 779.6 0 13 115 13 779.6 0 13 116 19 779.6 0 19 117 30 779.6 0 30 118 13 779.6 0 13 119 15 779.6 0 15 120 18 779.6 0 18 121 19 779.6 0 19 122 16 779.6 0 16 123 20 779.6 0 20 124 11 779.6 0 11 125 12 779.6 0 12 126 17 779.6 0 17 127 18 779.6 0 18 128 25 779.6 0 25 129 36 779.6 0 36 130 178 779.6 0 178 131 158 779.6 0 158 132 765 779.6 0 765 133 99 779.6 0 99 134 723 779.6 0 723 135 66 779.6 0 66 136 336 779.6 0 336 137 32 779.6 0 32 138 30 779.6 0 30 139 13 779.6 0 13 140 19 779.6 0 19 141 17 779.6 0 17 142 36 779.6 0 36 143 34 779.6 0 34 144 25 779.6 0 25 145 33 779.6 0 33 146 11 779.6 0 11 147 18 779.6 0 18 148 11 779.6 0 11 149 24 779.6 0 24 150 10 779.6 0 10 151 33 779.6 0 33 152 42 779.6 0 42 153 16 779.6 0 16 154 23 779.6 0 23 155 15 779.6 0 15 156 25 779.6 0 25 157 56 779.6 0 56 158 49 779.6 0 49 159 38 779.6 0 38 160 30 779.6 0 30 161 33 779.6 0 33 162 42 779.6 0 42 163 40 779.6 0 40 164 82 779.6 0 82 165 111 779.6 0 111 166 117 779.6 0 117 167 243 779.6 0 243 168 333 779.6 0 333 169 3172 779.6 0 3172 170 2813 779.6 0 2813 171 13251 779.6 0 13251 172 67 779.6 0 67 173 14 779.6 0 14 174 9 779.6 0 9 175 12 779.6 0 12 176 11 779.6 0 11 177 16 779.6 0 16 178 60 779.6 0 60 179 16 779.6 0 16 180 10 779.6 0 10 181 13 779.6 0 13 182 12 779.6 0 12 183 9 779.6 0 9 184 21 779.6 0 21 185 15 779.6 0 15 186 16 779.6 0 16 187 10 779.6 0 10 188 6 779.6 0 6 189 2 779.6 0 2 190 8 779.6 0 8 191 19 779.6 0 19 192 83 779.6 0 83 193 14 779.6 0 14 194 32 779.6 0 32 195 5 779.6 0 5 196 11 779.6 0 11 197 11 779.6 0 11 198 21 779.6 0 21 199 15 779.6 0 15 200 10 779.6 0 10 201 10 779.6 0 10 202 14 779.6 0 14 203 37 779.6 0 37 204 70 779.6 0 70 205 61 779.6 0 61 206 18 779.6 0 18 207 8 779.6 0 8 208 10 779.6 0 10 209 16 779.6 0 16 210 17 779.6 0 17 211 15 779.6 0 15 212 12 779.6 0 12 213 13 779.6 0 13 214 16 779.6 0 16 215 11 779.6 0 11 216 14 779.6 0 14 217 19 779.6 0 19 218 18 779.6 0 18 219 24 779.6 0 24 220 36 779.6 0 36 221 279 779.6 0 279 222 251 779.6 0 251 223 655 779.6 0 655 224 954 779.6 0 954 225 29 779.6 0 29 226 11 779.6 0 11 227 15 779.6 0 15 228 13 779.6 0 13 229 10 779.6 0 10 230 26 779.6 0 26 231 30 779.6 0 30 232 26 779.6 0 26 233 16 779.6 0 16 234 10 779.6 0 10 235 19 779.6 0 19 236 38 779.6 0 38 237 36 779.6 0 36 238 82 779.6 0 82 239 30 779.6 0 30 240 22 779.6 0 22 241 7 779.6 0 7 242 11 779.6 0 11 243 9 779.6 0 9 244 9 779.6 0 9 245 16 779.6 0 16 246 15 779.6 0 15 247 8 779.6 0 8 248 6 779.6 0 6 249 9 779.6 0 9 250 11 779.6 0 11 251 7 779.6 0 7 252 8 779.6 0 8 253 9 779.6 0 9 254 22 779.6 0 22 255 40 779.6 0 40 256 13 779.6 0 13 257 100 779.6 0 100 258 18 779.6 0 18 259 12 779.6 0 12 260 7 779.6 0 7 261 37 779.6 0 37 262 13 779.6 0 13 263 8 779.6 0 8 264 12 779.6 0 12 265 8 779.6 0 8 266 10 779.6 0 10 267 25 779.6 0 25 268 9 779.6 0 9 269 22 779.6 0 22 270 33 779.6 0 33 271 320 779.6 0 320 272 477 779.6 0 477 273 336 779.6 0 336 274 226 779.6 0 226 275 7 779.6 0 7 276 6 779.6 0 6 277 10 779.6 0 10 278 28 779.6 0 28 279 12 779.6 0 12 280 24 779.6 0 24 281 7 779.6 0 7 282 12 779.6 0 12 283 10 779.6 0 10 284 11 779.6 0 11 285 11 779.6 0 11 286 7 779.6 0 7 287 5 779.6 0 5 288 10 779.6 0 10 289 7 779.6 0 7 290 11 779.6 0 11 291 10 779.6 0 10 292 13 779.6 0 13 293 12 779.6 0 12 294 15 779.6 0 15 295 10 779.6 0 10 296 34 779.6 0 34 297 64 779.6 0 64 298 31 779.6 0 31 299 5 779.6 0 5 300 13 779.6 0 13 301 10 779.6 0 10 Finished in 2.55 s (46 us/read; 1.30 M reads/minute). === Summary === Total reads processed: 55,288 Reads with adapters: 14,673 (26.5%) Reads that were too short: 3 (0.0%) Reads written (passing filters): 14,670 (26.5%) Total basepairs processed: 12,269,414 bp Total written (filtered): 4,055,061 bp (33.1%) === Adapter 1 === Sequence: CCTAYGGGRBGCASCAG; Type: regular 5'; Length: 17; Trimmed: 14673 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-17 bp: 3 Overview of removed sequences length count expect max.err error counts 15 5 0.0 3 0 0 5 16 119 0.0 3 0 117 2 17 11012 0.0 3 10847 160 4 1 18 33 0.0 3 1 27 5 19 54 0.0 3 5 39 8 2 20 3425 0.0 3 3332 72 2 19 21 7 0.0 3 0 7 22 2 0.0 3 0 0 1 1 23 1 0.0 3 0 0 0 1 42 1 0.0 3 0 0 0 1 50 2 0.0 3 0 0 0 2 53 1 0.0 3 0 0 0 1 66 1 0.0 3 0 0 0 1 76 1 0.0 3 0 0 0 1 80 1 0.0 3 0 0 0 1 83 1 0.0 3 0 0 0 1 99 1 0.0 3 0 0 0 1 126 2 0.0 3 0 0 0 2 191 1 0.0 3 0 0 0 1 192 3 0.0 3 0 0 0 3 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a taatctatgggnncatcagg - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.62 s (42 us/read; 1.42 M reads/minute). === Summary === Total reads processed: 14,670 Reads with adapters: 1,107 (7.5%) Reads written (passing filters): 14,670 (100.0%) Total basepairs processed: 4,055,061 bp Total written (filtered): 4,051,733 bp (99.9%) === Adapter 1 === Sequence: TAATCTATGGGNNCATCAGG; Type: regular 3'; Length: 20; Trimmed: 1107 times. No. of allowed errors: 0-9 bp: 0; 10-19 bp: 1; 20 bp: 2 Bases preceding removed adapters: A: 12.6% C: 65.2% G: 21.3% T: 0.9% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 3 1101 229.2 0 1101 4 5 57.3 0 5 5 1 14.3 0 1