This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g TTCTCG -e 0 -O 6 - Trimming 1 adapter with at most 0.0% errors in single-end mode ... This is cutadapt 1.11 with Python 2.7.12 Command line parameters: --discard-untrimmed --minimum-length 100 -g ggactacnngggtatctaat -e 0.2 -O 20 - Trimming 1 adapter with at most 20.0% errors in single-end mode ... Finished in 100.32 s (31 us/read; 1.91 M reads/minute). === Summary === Total reads processed: 3,193,041 Reads with adapters: 33,496 (1.0%) Reads that were too short: 3,040 (0.1%) Reads written (passing filters): 30,456 (1.0%) Total basepairs processed: 961,105,341 bp Total written (filtered): 7,254,692 bp (0.8%) === Adapter 1 === Sequence: TTCTCG; Type: regular 5'; Length: 6; Trimmed: 33496 times. No. of allowed errors: 0-6 bp: 0 Overview of removed sequences length count expect max.err error counts 6 143 779.6 0 143 7 155 779.6 0 155 8 285 779.6 0 285 9 13286 779.6 0 13286 10 34 779.6 0 34 11 37 779.6 0 37 12 15 779.6 0 15 13 16 779.6 0 16 14 16 779.6 0 16 15 13 779.6 0 13 16 8 779.6 0 8 17 22 779.6 0 22 18 19 779.6 0 19 19 24 779.6 0 24 20 12 779.6 0 12 21 13 779.6 0 13 22 14 779.6 0 14 23 16 779.6 0 16 24 21 779.6 0 21 25 14 779.6 0 14 26 19 779.6 0 19 27 23 779.6 0 23 28 12 779.6 0 12 29 20 779.6 0 20 30 20 779.6 0 20 31 13 779.6 0 13 32 17 779.6 0 17 33 15 779.6 0 15 34 14 779.6 0 14 35 12 779.6 0 12 36 14 779.6 0 14 37 26 779.6 0 26 38 27 779.6 0 27 39 1006 779.6 0 1006 40 244 779.6 0 244 41 25 779.6 0 25 42 18 779.6 0 18 43 23 779.6 0 23 44 10 779.6 0 10 45 18 779.6 0 18 46 16 779.6 0 16 47 21 779.6 0 21 48 152 779.6 0 152 49 22 779.6 0 22 50 14 779.6 0 14 51 17 779.6 0 17 52 71 779.6 0 71 53 26 779.6 0 26 54 107 779.6 0 107 55 10 779.6 0 10 56 15 779.6 0 15 57 20 779.6 0 20 58 15 779.6 0 15 59 6 779.6 0 6 60 17 779.6 0 17 61 13 779.6 0 13 62 12 779.6 0 12 63 16 779.6 0 16 64 9 779.6 0 9 65 12 779.6 0 12 66 10 779.6 0 10 67 9 779.6 0 9 68 12 779.6 0 12 69 8 779.6 0 8 70 19 779.6 0 19 71 22 779.6 0 22 72 24 779.6 0 24 73 18 779.6 0 18 74 21 779.6 0 21 75 52 779.6 0 52 76 100 779.6 0 100 77 21 779.6 0 21 78 21 779.6 0 21 79 21 779.6 0 21 80 20 779.6 0 20 81 17 779.6 0 17 82 24 779.6 0 24 83 39 779.6 0 39 84 43 779.6 0 43 85 29 779.6 0 29 86 22 779.6 0 22 87 47 779.6 0 47 88 27 779.6 0 27 89 29 779.6 0 29 90 703 779.6 0 703 91 482 779.6 0 482 92 559 779.6 0 559 93 46 779.6 0 46 94 93 779.6 0 93 95 47 779.6 0 47 96 69 779.6 0 69 97 106 779.6 0 106 98 193 779.6 0 193 99 32 779.6 0 32 100 43 779.6 0 43 101 73 779.6 0 73 102 51 779.6 0 51 103 61 779.6 0 61 104 85 779.6 0 85 105 157 779.6 0 157 106 155 779.6 0 155 107 71 779.6 0 71 108 68 779.6 0 68 109 71 779.6 0 71 110 167 779.6 0 167 111 3209 779.6 0 3209 112 481 779.6 0 481 113 2703 779.6 0 2703 114 50 779.6 0 50 115 35 779.6 0 35 116 18 779.6 0 18 117 21 779.6 0 21 118 18 779.6 0 18 119 20 779.6 0 20 120 16 779.6 0 16 121 18 779.6 0 18 122 19 779.6 0 19 123 19 779.6 0 19 124 28 779.6 0 28 125 37 779.6 0 37 126 37 779.6 0 37 127 21 779.6 0 21 128 21 779.6 0 21 129 34 779.6 0 34 130 14 779.6 0 14 131 22 779.6 0 22 132 31 779.6 0 31 133 20 779.6 0 20 134 25 779.6 0 25 135 28 779.6 0 28 136 16 779.6 0 16 137 20 779.6 0 20 138 13 779.6 0 13 139 27 779.6 0 27 140 19 779.6 0 19 141 22 779.6 0 22 142 19 779.6 0 19 143 72 779.6 0 72 144 52 779.6 0 52 145 57 779.6 0 57 146 17 779.6 0 17 147 12 779.6 0 12 148 18 779.6 0 18 149 21 779.6 0 21 150 20 779.6 0 20 151 10 779.6 0 10 152 16 779.6 0 16 153 38 779.6 0 38 154 29 779.6 0 29 155 21 779.6 0 21 156 30 779.6 0 30 157 18 779.6 0 18 158 28 779.6 0 28 159 52 779.6 0 52 160 54 779.6 0 54 161 658 779.6 0 658 162 178 779.6 0 178 163 382 779.6 0 382 164 74 779.6 0 74 165 68 779.6 0 68 166 13 779.6 0 13 167 20 779.6 0 20 168 28 779.6 0 28 169 25 779.6 0 25 170 29 779.6 0 29 171 37 779.6 0 37 172 40 779.6 0 40 173 29 779.6 0 29 174 17 779.6 0 17 175 11 779.6 0 11 176 24 779.6 0 24 177 46 779.6 0 46 178 22 779.6 0 22 179 40 779.6 0 40 180 30 779.6 0 30 181 8 779.6 0 8 182 16 779.6 0 16 183 18 779.6 0 18 184 14 779.6 0 14 185 22 779.6 0 22 186 17 779.6 0 17 187 18 779.6 0 18 188 32 779.6 0 32 189 44 779.6 0 44 190 103 779.6 0 103 191 70 779.6 0 70 192 17 779.6 0 17 193 22 779.6 0 22 194 17 779.6 0 17 195 24 779.6 0 24 196 36 779.6 0 36 197 37 779.6 0 37 198 31 779.6 0 31 199 36 779.6 0 36 200 42 779.6 0 42 201 98 779.6 0 98 202 43 779.6 0 43 203 27 779.6 0 27 204 33 779.6 0 33 205 40 779.6 0 40 206 19 779.6 0 19 207 15 779.6 0 15 208 18 779.6 0 18 209 17 779.6 0 17 210 28 779.6 0 28 211 73 779.6 0 73 212 26 779.6 0 26 213 50 779.6 0 50 214 28 779.6 0 28 215 35 779.6 0 35 216 39 779.6 0 39 217 45 779.6 0 45 218 16 779.6 0 16 219 14 779.6 0 14 220 15 779.6 0 15 221 9 779.6 0 9 222 17 779.6 0 17 223 56 779.6 0 56 224 82 779.6 0 82 225 138 779.6 0 138 226 54 779.6 0 54 227 18 779.6 0 18 228 22 779.6 0 22 229 25 779.6 0 25 230 23 779.6 0 23 231 25 779.6 0 25 232 13 779.6 0 13 233 17 779.6 0 17 234 26 779.6 0 26 235 28 779.6 0 28 236 19 779.6 0 19 237 15 779.6 0 15 238 23 779.6 0 23 239 14 779.6 0 14 240 9 779.6 0 9 241 16 779.6 0 16 242 73 779.6 0 73 243 61 779.6 0 61 244 153 779.6 0 153 245 286 779.6 0 286 246 60 779.6 0 60 247 48 779.6 0 48 248 14 779.6 0 14 249 13 779.6 0 13 250 19 779.6 0 19 251 11 779.6 0 11 252 18 779.6 0 18 253 31 779.6 0 31 254 53 779.6 0 53 255 52 779.6 0 52 256 37 779.6 0 37 257 9 779.6 0 9 258 9 779.6 0 9 259 10 779.6 0 10 260 17 779.6 0 17 261 16 779.6 0 16 262 12 779.6 0 12 263 17 779.6 0 17 264 27 779.6 0 27 265 26 779.6 0 26 266 36 779.6 0 36 267 12 779.6 0 12 268 9 779.6 0 9 269 23 779.6 0 23 270 14 779.6 0 14 271 11 779.6 0 11 272 5 779.6 0 5 273 7 779.6 0 7 274 5 779.6 0 5 275 8 779.6 0 8 276 10 779.6 0 10 277 12 779.6 0 12 278 6 779.6 0 6 279 17 779.6 0 17 280 9 779.6 0 9 281 7 779.6 0 7 282 15 779.6 0 15 283 16 779.6 0 16 284 14 779.6 0 14 285 5 779.6 0 5 286 8 779.6 0 8 287 34 779.6 0 34 288 23 779.6 0 23 289 15 779.6 0 15 290 12 779.6 0 12 291 21 779.6 0 21 292 12 779.6 0 12 293 11 779.6 0 11 294 11 779.6 0 11 295 25 779.6 0 25 296 88 779.6 0 88 297 160 779.6 0 160 298 38 779.6 0 38 299 23 779.6 0 23 300 9 779.6 0 9 301 7 779.6 0 7 Finished in 1.57 s (52 us/read; 1.16 M reads/minute). === Summary === Total reads processed: 30,456 Reads with adapters: 13,766 (45.2%) Reads that were too short: 4 (0.0%) Reads written (passing filters): 13,762 (45.2%) Total basepairs processed: 7,254,692 bp Total written (filtered): 3,743,405 bp (51.6%) === Adapter 1 === Sequence: GGACTACNNGGGTATCTAAT; Type: regular 5'; Length: 20; Trimmed: 13766 times. No. of allowed errors: 0-4 bp: 0; 5-9 bp: 1; 10-14 bp: 2; 15-19 bp: 3; 20 bp: 4 Overview of removed sequences length count expect max.err error counts 17 1 0.0 3 0 0 0 1 18 4 0.0 3 0 0 2 0 2 19 98 0.0 3 0 92 5 0 1 20 13534 0.0 4 13081 361 72 11 9 21 60 0.0 4 18 38 4 22 7 0.0 4 1 0 5 1 23 53 0.0 4 41 1 0 9 2 24 1 0.0 4 0 0 0 1 32 1 0.0 4 0 0 0 0 1 56 3 0.0 4 0 0 0 0 3 105 1 0.0 4 0 0 0 0 1 179 1 0.0 4 0 0 0 0 1 187 1 0.0 4 0 0 0 0 1 195 1 0.0 4 0 0 0 0 1 This is cutadapt 1.11 with Python 2.7.12 Command line parameters: -a CTGSTGCVYCCCRTAGG - Trimming 1 adapter with at most 10.0% errors in single-end mode ... Finished in 0.60 s (44 us/read; 1.38 M reads/minute). === Summary === Total reads processed: 13,762 Reads with adapters: 1,128 (8.2%) Reads written (passing filters): 13,762 (100.0%) Total basepairs processed: 3,743,405 bp Total written (filtered): 3,646,046 bp (97.4%) === Adapter 1 === Sequence: CTGSTGCVYCCCRTAGG; Type: regular 3'; Length: 17; Trimmed: 1128 times. No. of allowed errors: 0-9 bp: 0; 10-17 bp: 1 Bases preceding removed adapters: A: 32.7% C: 1.6% G: 63.7% T: 2.0% none/other: 0.0% Overview of removed sequences length count expect max.err error counts 4 2 53.8 0 2 5 1 13.4 0 1 8 1 0.2 0 1 9 157 0.1 0 11 146 10 285 0.0 1 5 280 11 323 0.0 1 2 321 12 3 0.0 1 0 3 13 4 0.0 1 0 4 122 3 0.0 1 3 150 1 0.0 1 1 208 1 0.0 1 1 228 16 0.0 1 16 232 1 0.0 1 1 254 1 0.0 1 1 257 313 0.0 1 294 19 258 12 0.0 1 12 259 1 0.0 1 1 260 3 0.0 1 2 1